##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921368_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4082474
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.439955526967225	33.0	31.0	34.0	29.0	34.0
2	31.563561947975664	34.0	31.0	34.0	29.0	34.0
3	31.646678950068022	34.0	31.0	34.0	28.0	34.0
4	35.12739627980484	37.0	35.0	37.0	33.0	37.0
5	34.86948330840564	37.0	35.0	37.0	32.0	37.0
6	34.83110119011168	37.0	35.0	37.0	32.0	37.0
7	34.69167617479009	37.0	35.0	37.0	32.0	37.0
8	34.65636449858591	37.0	35.0	37.0	32.0	37.0
9	36.287715243256905	39.0	37.0	39.0	32.0	39.0
10-11	36.13529345196075	39.0	37.0	39.0	32.0	39.0
12-13	36.061554954177296	39.0	37.0	39.0	32.0	39.0
14-15	37.325040159471925	40.0	38.0	41.0	32.0	41.0
16-17	37.30228251300559	40.0	38.0	41.0	31.5	41.0
18-19	37.24753948218654	40.0	38.0	41.0	31.0	41.0
20-21	37.20200863496008	40.0	38.0	41.0	31.0	41.0
22-23	37.09621494221396	40.0	38.0	41.0	31.0	41.0
24-25	37.03142567472567	40.0	37.5	41.0	31.0	41.0
26-27	36.90035784673705	40.0	37.0	41.0	30.0	41.0
28-29	36.74797414508947	40.0	37.0	41.0	30.0	41.0
30-31	36.62355424676312	40.0	37.0	41.0	30.0	41.0
32-33	36.59075122095082	40.0	37.0	41.0	30.0	41.0
34-35	36.744461446662	40.0	37.0	41.0	30.0	41.0
36-37	36.73185083358767	40.0	37.0	41.0	30.0	41.0
38-39	36.65302240749114	40.0	37.0	41.0	30.0	41.0
40-41	36.53601149695014	40.0	37.0	41.0	30.0	41.0
42-43	36.443902765822884	40.0	37.0	41.0	30.0	41.0
44-45	36.32046964659175	40.0	36.5	41.0	29.0	41.0
46-47	36.1835274394889	40.0	36.0	41.0	29.0	41.0
48-49	36.033550489237655	40.0	36.0	41.0	28.0	41.0
50-51	35.87564133905079	40.0	35.0	41.0	28.0	41.0
52-53	35.69631907025005	39.0	35.0	41.0	28.0	41.0
54-55	35.47942950769558	39.0	35.0	41.0	27.5	41.0
56-57	35.20627614529817	39.0	35.0	41.0	27.0	41.0
58-59	34.969170164953894	38.5	35.0	40.5	26.0	41.0
60-61	34.68371286626687	38.0	34.5	40.0	26.0	41.0
62-63	34.37234199164526	37.0	34.0	40.0	26.0	41.0
64-65	34.04036914870738	37.0	34.0	40.0	26.0	41.0
66-67	33.692914884454865	36.0	34.0	39.0	25.5	41.0
68-69	33.32612124412795	36.0	34.0	39.0	25.0	40.5
70-71	32.94484128986492	35.0	33.0	38.5	24.0	40.0
72-73	32.5536666002037	35.0	33.0	37.0	23.0	39.5
74-75	32.154694555311316	35.0	33.0	37.0	22.0	39.0
76-77	29.43503939033047	33.0	29.0	34.5	17.5	36.5
78-79	31.096449848792666	34.5	31.5	35.5	20.0	37.0
80-81	31.304266481550158	35.0	32.0	35.0	20.0	37.0
82-83	31.160367708404266	35.0	32.0	35.0	20.0	37.0
84-85	30.94895791130574	35.0	32.0	35.0	19.5	36.0
86-87	30.722277104520444	35.0	32.0	35.0	18.0	36.0
88-89	30.46337576185421	35.0	32.0	35.0	13.5	35.5
90-91	30.298875632765817	35.0	32.0	35.0	9.0	35.0
92-93	30.168170697474128	34.0	32.0	35.0	7.0	35.0
94-95	30.079593648361264	34.0	32.0	35.0	2.0	35.0
96-97	29.904532153787137	34.0	31.5	35.0	2.0	35.0
98-99	29.653101771131915	34.0	31.0	35.0	2.0	35.0
100-101	28.579290400869667	33.5	29.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	74416.0
3	25901.0
4	14327.0
5	9960.0
6	8911.0
7	8493.0
8	8847.0
9	9656.0
10	10474.0
11	11332.0
12	11942.0
13	12381.0
14	13173.0
15	14204.0
16	14756.0
17	15548.0
18	15085.0
19	14119.0
20	13456.0
21	13655.0
22	14647.0
23	16736.0
24	19654.0
25	23884.0
26	29192.0
27	35044.0
28	41976.0
29	50988.0
30	62866.0
31	78114.0
32	99902.0
33	132381.0
34	191110.0
35	291102.0
36	524291.0
37	1029606.0
38	1007932.0
39	112410.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.33610966791427	18.268884986942542	12.48896751654198	26.906037828601203
2	15.621384681521292	19.647809957488814	37.00285734931555	27.727948011674346
3	18.772758706089245	22.96870165784428	27.92097160644344	30.33756802962303
4	13.253095276180968	15.268543057794576	33.785426683052975	37.69293498297148
5	14.641748898975235	36.740746185462186	33.86456935996619	14.752935555596391
6	36.229101897204316	33.86839890378961	15.65668763784689	14.245811561159192
7	30.855193057144326	30.59679556738935	19.515366922475856	19.032644452990468
8	27.89459979752211	34.98198740404285	18.334194342033808	18.789218456401237
9	26.840961348672725	15.235348770218444	18.094914528522295	39.82877535258653
10-11	26.764050610751138	24.774170358111753	26.62429202776586	21.837487003371248
12-13	26.854330270355074	21.749043990462134	26.912224221423113	24.484401517759675
14-15	23.691776650840442	23.55334822379989	23.6698892327621	29.08498589259757
16-17	24.50131256419219	26.831680452040395	24.499352964763705	24.167654019003706
18-19	24.552831110246707	26.195626560639145	25.658830382254628	23.592711946859517
20-21	24.85269548673595	25.33108533379939	25.76924193335635	24.046977246108305
22-23	24.599702532588164	25.08177659374339	25.618964429325036	24.699556444343415
24-25	24.475933017799832	25.348447162672528	25.53848252914096	24.63713729038668
26-27	24.342796057615324	25.57114447396311	25.566882224619807	24.519177243801764
28-29	24.427935880511225	25.78097154199791	25.3798756599091	24.41121691758176
30-31	24.691528456577206	25.47182233979391	25.310658046902397	24.525991156726487
32-33	24.41696352866326	25.47972580167438	25.34326850264205	24.760042167020305
34-35	24.626109349587423	25.56493628504984	25.485967816337496	24.322986549025245
36-37	24.679285251677673	25.467048660067356	25.51289129746789	24.340774790787087
38-39	24.594340938671436	25.606414169490638	25.420778616038387	24.37846627579954
40-41	24.667743435675384	25.38846909976289	25.522009850146038	24.42177761441569
42-43	24.49397041686397	25.370891567941484	25.40079022606619	24.734347789128353
44-45	24.389187841190125	25.4399637051914	25.536200311283835	24.63464814233464
46-47	24.573264337199962	25.4106592387259	25.43918647047346	24.576889953600684
48-49	24.446289679372942	25.54879237077153	25.40350768026981	24.60141026958572
50-51	24.44358053955433	25.56300285664278	25.521591763540574	24.471824840262315
52-53	24.71346800501421	25.422083469333888	25.399915123404813	24.464533402247085
54-55	24.199592571739938	25.309579218194823	25.727050500356835	24.763777709708407
56-57	24.323448742898947	25.47277168027912	25.536903302472336	24.66687627434959
58-59	24.35655541325379	25.43187414688351	25.570332564773164	24.641237875089537
60-61	24.41706135131335	25.505038751524317	25.640702157337213	24.437197739825123
62-63	24.449838695135767	25.46327374395842	25.585293621891992	24.501593939013823
64-65	24.1699488909047	25.315382666425553	25.634404080886554	24.880264361783198
66-67	24.209016352267835	25.477221920414372	25.521316904857994	24.792444822459796
68-69	24.286452833239817	25.363137867921804	25.589311922222198	24.761097376616185
70-71	24.16118845659362	25.224438855382974	25.62308730285826	24.991285385165146
72-73	24.22767259431077	25.394143475048537	25.435562948963636	24.94262098167706
74-75	24.003978963435923	25.509651558937783	25.5764290518233	24.90994042580299
76-77	24.10394393934316	25.1787312155294	25.60069856078359	25.11662628434385
78-79	23.996445871542768	25.244729319415036	25.679889454349365	25.078935354692828
80-81	24.136677732151547	25.379810820647243	25.454953183073115	25.028558264128097
82-83	24.0859300648039	25.370566575073166	25.544933551530928	24.998569808592002
84-85	24.037968066237923	25.3475691325017	25.476502857700122	25.137959943560258
86-87	23.85962865099933	25.523995647685084	25.479060459890686	25.1373152414249
88-89	23.73798914505688	25.321262625450185	25.5568226764401	25.383925553052833
90-91	23.80376230969916	25.231837914641286	25.6074054462145	25.356994329445055
92-93	23.843575709244973	25.132521628657116	25.664213352102433	25.359689309995474
94-95	23.746347170411244	25.01419243484126	25.725326811815112	25.514133582932384
96-97	23.606154860712863	24.92998101225666	25.7776192539784	25.68624487305208
98-99	23.432626222493738	24.934561097619703	25.754206926554108	25.87860575333245
100-101	23.43515247150678	24.94075509406985	25.794954632897145	25.829137801526226
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	22.0
1	19.5
2	30.5
3	50.0
4	47.0
5	46.5
6	105.0
7	246.0
8	371.5
9	391.0
10	329.0
11	276.5
12	273.5
13	294.0
14	337.0
15	385.5
16	426.0
17	452.5
18	479.0
19	547.5
20	658.0
21	764.5
22	873.5
23	1010.5
24	1258.0
25	1696.0
26	2302.0
27	3239.0
28	4603.0
29	6947.5
30	10633.0
31	15523.5
32	23256.5
33	29974.0
34	35362.5
35	43255.0
36	54130.5
37	65733.0
38	76831.0
39	87904.5
40	97775.0
41	107158.5
42	117161.0
43	128522.5
44	139621.0
45	151838.5
46	161356.0
47	168885.5
48	177393.5
49	182682.5
50	185723.5
51	188614.0
52	191011.5
53	191578.5
54	190235.0
55	183494.5
56	171155.0
57	155801.5
58	140200.5
59	123774.5
60	104173.5
61	86259.0
62	70718.5
63	54077.5
64	38505.5
65	27558.5
66	20280.5
67	15352.0
68	11556.5
69	8442.0
70	6265.5
71	4454.0
72	2999.5
73	2151.0
74	1628.0
75	1141.5
76	762.0
77	466.5
78	249.0
79	126.0
80	74.0
81	52.5
82	42.0
83	26.5
84	16.0
85	11.0
86	9.0
87	6.0
88	5.0
89	3.5
90	1.5
91	1.5
92	1.0
93	0.5
94	0.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14393233122856386
2	4.654040662598219E-4
3	0.006001262959666124
4	0.00502146492543492
5	0.025695203447713325
6	0.0011267677393658844
7	9.79798034231204E-5
8	0.0012982323953563452
9	2.44949508557801E-5
10-11	0.09828599030881766
12-13	2.3270203312991095E-4
14-15	0.005388889188271621
16-17	1.7146465599046068E-4
18-19	2.939394102693612E-4
20-21	7.226010502455128E-4
22-23	2.4494950855780096E-4
24-25	0.0018616162650392876
26-27	0.003000631479833062
28-29	0.006932071092185767
30-31	0.015052147300876872
32-33	0.01496641497288164
34-35	0.011390152147937746
36-37	7.348485256734029E-4
38-39	3.0618688569725123E-4
40-41	0.00488674269572813
42-43	0.008597727750378814
44-45	0.008511995422383584
46-47	0.010030682375441951
48-49	0.011965783493048578
50-51	0.005744065975680433
52-53	0.0018738637404671775
54-55	9.185606570917537E-4
56-57	0.005989015484238234
58-59	0.009357071226907997
60-61	0.007642424667003391
62-63	0.018665152552104436
64-65	0.03429293119809214
66-67	0.03701187074308373
68-69	0.027042425744781228
70-71	0.04128623966741735
72-73	0.025976895382554797
74-75	0.024715405413482116
76-77	0.23340013922930067
78-79	0.06641805924544775
80-81	0.054354295948976034
82-83	0.020881945604552532
84-85	0.03202714824393248
86-87	0.07998826201954992
88-89	0.2618142822220056
90-91	0.31271479010031666
92-93	0.04918586131840644
94-95	0.004739772990593449
96-97	0.008989646964071297
98-99	9.797980342312038E-4
100-101	0.0013594697724957955
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4082474.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.00473425011257
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.78985507022857	38.894265375316714
2	12.968059473940322	13.488009733638423
3	4.625064939590876	7.215758192188093
4	2.2260744792665865	4.630656468608663
5	1.2798116422471555	3.3278132172631736
6	0.8283931221608145	2.5848218503556515
7	0.5690778666677346	2.0716320256573577
8	0.4134946723436425	1.7202944439254801
9	0.3217184413791771	1.5057793842556073
>10	1.8366214441076945	17.275149644412842
>50	0.10605721274964905	3.7311817966534933
>100	0.03416542159917432	2.78357565930221
>500	9.920732140743084E-4	0.35188543717767984
>1k	6.141405044752619E-4	0.41917677124463276
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.44949508557801E-5
2	0.0	0.0	0.0	0.0	2.44949508557801E-5
3	0.0	0.0	0.0	2.44949508557801E-5	2.44949508557801E-5
4	0.0	0.0	0.0	2.44949508557801E-5	2.44949508557801E-5
5	0.0	0.0	0.0	2.44949508557801E-5	4.89899017115602E-5
6	0.0	0.0	0.0	2.44949508557801E-5	4.89899017115602E-5
7	0.0	0.0	0.0	2.44949508557801E-5	4.89899017115602E-5
8	0.0	0.0	0.0	2.44949508557801E-5	4.89899017115602E-5
9	0.0	0.0	0.0	2.44949508557801E-5	7.34848525673403E-5
10-11	0.0	0.0	0.0	2.44949508557801E-5	7.34848525673403E-5
12-13	0.0	0.0	0.0	2.44949508557801E-5	1.8371213141835075E-4
14-15	0.0	0.0	0.0	2.44949508557801E-5	2.0820708227413083E-4
16-17	0.0	0.0	0.0	2.44949508557801E-5	2.694444594135811E-4
18-19	0.0	1.224747542789005E-5	0.0	2.44949508557801E-5	2.939394102693612E-4
20-21	0.0	2.44949508557801E-5	0.0	6.123737713945024E-5	2.939394102693612E-4
22-23	0.0	2.44949508557801E-5	0.0	1.1022727885101044E-4	3.306818365530313E-4
24-25	0.0	2.44949508557801E-5	0.0	1.959596068462408E-4	3.7967173826459154E-4
26-27	0.0	2.44949508557801E-5	0.0	4.041666891203716E-4	4.1641416454826167E-4
28-29	0.0	2.44949508557801E-5	0.0	0.002241288003303879	4.1641416454826167E-4
30-31	0.0	2.44949508557801E-5	0.0	0.009589773260037909	4.1641416454826167E-4
32-33	0.0	2.44949508557801E-5	0.0	0.023196718460423753	4.6540406625982184E-4
34-35	0.0	2.44949508557801E-5	0.0	0.041298487142845244	5.02146492543492E-4
36-37	0.0	2.44949508557801E-5	0.0	0.07093737767833916	5.14393967971382E-4
38-39	0.0	2.44949508557801E-5	0.0	0.12213182496691957	5.388889188271622E-4
40-41	0.0	2.44949508557801E-5	0.0	0.18112791410306595	5.388889188271622E-4
42-43	0.0	2.44949508557801E-5	0.0	0.24106705884715984	5.388889188271622E-4
44-45	0.0	2.44949508557801E-5	0.0	0.314123249774524	5.388889188271622E-4
46-47	0.0	2.44949508557801E-5	0.0	0.401006840459976	5.633838696829423E-4
48-49	0.0	2.44949508557801E-5	0.0	0.49732098722490337	5.756313451108323E-4
50-51	0.0	2.44949508557801E-5	0.0	0.6042781901366672	6.368687222502825E-4
52-53	0.0	2.44949508557801E-5	0.0	0.7122886759352294	6.858586239618427E-4
54-55	0.0	2.44949508557801E-5	0.0	0.83296305132623	7.103535748176228E-4
56-57	0.0	2.44949508557801E-5	0.0	0.9781078826221551	7.103535748176228E-4
58-59	0.0	2.44949508557801E-5	0.0	1.1339937498683397	7.103535748176228E-4
60-61	0.0	2.44949508557801E-5	0.0	1.3056666129410743	7.103535748176228E-4
62-63	0.0	2.44949508557801E-5	0.0	1.4844675067128414	7.838384273849632E-4
64-65	0.0	2.44949508557801E-5	0.0	1.6797412549351203	7.838384273849632E-4
66-67	0.0	2.44949508557801E-5	0.0	1.9040415199214986	7.838384273849632E-4
68-69	0.0	2.44949508557801E-5	0.0	2.1503137558255117	7.838384273849632E-4
70-71	0.0	2.44949508557801E-5	0.0	2.4171127605466687	7.838384273849632E-4
72-73	0.0	2.44949508557801E-5	0.0	2.7012909329000014	8.328283290965233E-4
74-75	1.224747542789005E-5	2.44949508557801E-5	0.0	2.9942627926105594	8.450758045244134E-4
76-77	2.44949508557801E-5	2.44949508557801E-5	0.0	3.3200456389924344	8.695707553801935E-4
78-79	2.44949508557801E-5	2.44949508557801E-5	0.0	3.690299068652979	8.818182308080835E-4
80-81	2.44949508557801E-5	2.44949508557801E-5	0.0	4.083736969298519	9.185606570917537E-4
82-83	2.44949508557801E-5	2.44949508557801E-5	0.0	4.496207446759	9.797980342312038E-4
84-85	2.44949508557801E-5	2.44949508557801E-5	0.0	4.925885627195666	9.797980342312038E-4
86-87	2.44949508557801E-5	2.44949508557801E-5	0.0	5.384761789052423	0.0010165404605148739
88-89	2.44949508557801E-5	2.44949508557801E-5	0.0	5.887030756350193	0.001028787935942764
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2710	0.0	40.510746	1
GTATCAA	4325	0.0	35.932644	1
GTACATG	10720	0.0	29.082827	1
TCAACGC	5275	0.0	28.880747	4
CAACGCA	5515	0.0	27.755209	5
ATCAACG	5560	0.0	27.40035	3
TACATGG	11390	0.0	26.750824	2
TATCAAC	5990	0.0	25.591843	2
AACGCAG	5990	0.0	25.55237	6
ACATGGG	11870	0.0	25.149296	3
CATGGGG	10045	0.0	23.15112	4
ATGGGGG	6070	0.0	20.205292	5
ACGCAGA	7615	0.0	19.537601	7
TATACCG	320	3.929017E-10	19.312012	5
GAGTACA	7855	0.0	18.937634	1
CGCAGAG	7910	0.0	18.388308	8
TCTACAC	2345	0.0	16.595749	3
AGTACAT	8500	0.0	16.527176	2
GTATAGG	1130	0.0	16.40264	1
TATACAG	1980	0.0	16.085838	5
>>END_MODULE