FastQCFastQC Report
Mon 15 Aug 2016
SRR2921367_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921367_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4246818
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28750.043.5782931
GTATCAA51500.042.020551
TCAACGC62750.034.037624
CAACGCA63700.033.5288125
ATCAACG65550.032.2917753
GTACATG132050.031.949751
TATCAAC67600.031.593712
AACGCAG68450.031.1907256
TACATGG138600.029.825642
ACATGGG141500.028.0400983
CATGGGG123900.025.3598754
ACGCAGA86050.024.2087147
CGCAGAG90250.022.7693148
ATGGGGG84250.021.6324835
GAGTACA92800.021.5808531
AGTACAT96850.019.4547542
TACACTG53150.018.841885
GTATAGC10000.018.5083941
GCAGAGT103800.018.471129
GTAAGTA21950.018.1626662
Produced by FastQC (version 0.11.5)