##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921367_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4246818
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.477161959848527	31.0	31.0	34.0	26.0	34.0
2	30.51645914658928	33.0	31.0	34.0	26.0	34.0
3	30.570730368007293	33.0	31.0	34.0	26.0	34.0
4	34.03638041470108	37.0	35.0	37.0	30.0	37.0
5	34.03254271786547	37.0	35.0	37.0	30.0	37.0
6	34.07667010924415	37.0	35.0	37.0	30.0	37.0
7	33.98820222576056	37.0	35.0	37.0	30.0	37.0
8	33.950937148707574	37.0	35.0	37.0	30.0	37.0
9	35.51976656404866	39.0	37.0	39.0	30.0	39.0
10-11	35.41400055288453	39.0	37.0	39.0	30.0	39.0
12-13	35.2923077937411	39.0	35.0	39.0	29.0	39.0
14-15	36.43802760090025	40.0	37.0	41.0	29.0	41.0
16-17	36.384970935886585	40.0	37.0	41.0	29.0	41.0
18-19	36.31995296242975	40.0	37.0	41.0	28.0	41.0
20-21	36.25227900041867	40.0	37.0	41.0	27.0	41.0
22-23	36.14138562095197	40.0	36.0	41.0	27.0	41.0
24-25	36.04122251059499	40.0	36.0	41.0	27.0	41.0
26-27	35.896429279521755	40.0	36.0	41.0	27.0	41.0
28-29	35.75729557989064	40.0	36.0	41.0	27.0	41.0
30-31	35.61775710661488	40.0	36.0	41.0	26.0	41.0
32-33	35.44906939736998	39.5	35.0	41.0	25.0	41.0
34-35	35.292614140751965	39.0	35.0	41.0	25.0	41.0
36-37	35.130879519678025	39.0	35.0	41.0	24.5	41.0
38-39	35.37865455971977	39.5	35.0	41.0	25.0	41.0
40-41	35.508299154802486	40.0	35.0	41.0	25.0	41.0
42-43	35.35715681717465	40.0	35.0	41.0	24.5	41.0
44-45	35.25114297339796	40.0	35.0	41.0	24.0	41.0
46-47	35.11033861116723	40.0	35.0	41.0	24.0	41.0
48-49	34.972770554330324	39.5	35.0	41.0	23.0	41.0
50-51	33.24023198074417	37.0	32.5	39.5	19.5	40.0
52-53	33.395426999697186	37.5	33.5	39.5	20.5	40.0
54-55	34.263125238708135	39.0	34.0	40.5	21.5	41.0
56-57	34.27612320565656	39.0	34.0	41.0	21.0	41.0
58-59	34.093395219668	38.0	34.0	41.0	20.5	41.0
60-61	33.84118521208114	38.0	34.0	40.0	20.0	41.0
62-63	33.545023003104916	37.0	34.0	40.0	18.5	41.0
64-65	33.24716705542832	37.0	33.5	40.0	18.0	41.0
66-67	32.90541859811275	36.0	33.0	39.0	15.5	41.0
68-69	32.53945589380096	36.0	33.0	39.0	12.5	41.0
70-71	32.156030585723244	35.0	33.0	38.5	9.5	40.5
72-73	31.80265189136902	35.0	32.0	37.5	7.5	40.0
74-75	31.40882797426214	35.0	32.0	37.0	4.0	39.0
76-77	31.036774003500973	35.0	32.0	36.5	2.0	39.0
78-79	30.703122078695152	35.0	32.0	36.0	2.0	38.0
80-81	30.363779187146704	35.0	31.0	35.5	2.0	37.0
82-83	30.072501811944846	35.0	31.0	35.0	2.0	37.0
84-85	29.843115716284522	35.0	31.0	35.0	2.0	36.0
86-87	29.62639863540185	35.0	31.0	35.0	2.0	36.0
88-89	29.433281341465538	35.0	31.0	35.0	2.0	36.0
90-91	29.18596641061614	35.0	31.0	35.0	2.0	35.0
92-93	28.990331349259613	34.0	30.0	35.0	2.0	35.0
94-95	28.778609302305867	34.0	30.0	35.0	2.0	35.0
96-97	28.52686670349424	34.0	30.0	35.0	2.0	35.0
98-99	28.299923613397137	34.0	30.0	35.0	2.0	35.0
100-101	27.244634924312745	33.5	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	140810.0
3	24775.0
4	17222.0
5	13015.0
6	11852.0
7	11987.0
8	12606.0
9	13369.0
10	14299.0
11	15398.0
12	15385.0
13	15627.0
14	15806.0
15	17059.0
16	16816.0
17	17308.0
18	16786.0
19	16367.0
20	16867.0
21	17802.0
22	19560.0
23	22470.0
24	26776.0
25	31275.0
26	37459.0
27	45034.0
28	53170.0
29	63901.0
30	75737.0
31	92834.0
32	116252.0
33	151638.0
34	215530.0
35	308314.0
36	511814.0
37	926783.0
38	949545.0
39	157557.0
40	13.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.34644135198234	17.07430154568135	12.041072105881883	25.538184996454426
2	16.46500410895904	19.03767425379042	38.759099082458135	25.738222554792404
3	19.910958200581046	22.792071519911193	28.57853988034154	28.718430399166216
4	13.678220090305421	14.551282428845902	35.33663094580379	36.43386653504489
5	14.60192960708179	37.10818549369323	33.81059941076168	14.4792854884633
6	35.24624563661088	33.74487709502733	16.565688347448944	14.44318892091285
7	30.312402561129204	31.192072444122488	20.026908435957168	18.46861655879114
8	27.728173248033222	34.51460988952499	19.227004626441467	18.530212236000327
9	27.68087106760044	15.139043543959406	18.388001153083085	38.79208423535707
10-11	26.63636078871748	24.62269170177283	27.06528909678466	21.675658412725028
12-13	26.798798124029606	21.856251601621974	27.113505064893957	24.23144520945447
14-15	23.85906829589071	23.606854312237214	24.050528544538153	28.48354884733392
16-17	24.42291813902207	26.720057546548414	24.949026717384196	23.907997597045323
18-19	24.476367993107658	26.282192142817273	26.005664794966783	23.235775069108286
20-21	24.756465833733635	25.444175535613827	26.110346099443454	23.68901253120908
22-23	24.73094823256476	25.105090616804887	25.802208198622917	24.36175295200744
24-25	24.515123775530586	25.28832038037231	25.766082527071188	24.430473317025918
26-27	24.2201445679608	25.61157909823859	25.824225456016087	24.34405087778452
28-29	24.33408181270493	25.977486268064897	25.479657988733713	24.20877393049646
30-31	24.854739903621127	25.511376180817834	25.459707213107997	24.17417670245304
32-33	24.51702907368937	25.555238031654813	25.42133635801342	24.506396536642395
34-35	24.686396825365055	25.663905000212534	25.607539734450953	24.042158439971463
36-37	24.67385112922637	25.667279761945412	25.67461477075331	23.984254338074912
38-39	24.65900684311742	25.71535179491865	25.53077754434533	24.0948638176186
40-41	24.757786347118586	25.45712352095669	25.560098467879726	24.224991664045
42-43	24.41131541044755	25.527511982044395	25.448665977034494	24.61250663047356
44-45	24.23990330851291	25.554997647467903	25.718925905441775	24.48617313857741
46-47	24.437666829724186	25.378135579005377	25.567906694138337	24.616290897132096
48-49	24.306432935888154	25.575817397367288	25.660191388474107	24.457558278270447
50-51	24.463259156385895	25.520056249904897	25.713406451447007	24.3032781422622
52-53	24.657971180661473	25.33876134738747	25.724558956164213	24.278708515786843
54-55	23.962787594559455	25.199564074892393	26.12723195459849	24.710416375949666
56-57	24.13355477008792	25.391310974638664	25.770736514721072	24.70439774055235
58-59	24.348808894799756	25.411337420371705	25.813789077158155	24.426064607670387
60-61	24.521076051307478	25.391159417879145	25.924459153069623	24.163305377743754
62-63	24.45734123215704	25.319416096075038	25.96569206997768	24.257550601790236
64-65	24.113921116344077	25.122755450662886	26.044080481662153	24.719242951330884
66-67	24.24229341620595	25.326316243922655	25.76772712147286	24.663663218398533
68-69	24.156399410734945	25.2890679158765	25.946972042701784	24.607560630686773
70-71	24.125584146869596	25.07938406176554	25.97923913956216	24.815792651802706
72-73	24.09602061891419	25.49354367418775	25.711319868338816	24.699115838559244
74-75	24.097072331873157	25.519766654636122	25.845523104540703	24.537637908950018
76-77	24.081951439521372	25.065229067785154	25.951096539492095	24.90172295320137
78-79	23.967261461302247	25.099998572502315	26.06561227676325	24.867127689432188
80-81	24.224189549281267	25.341798332524156	25.70257153256639	24.73144058562818
82-83	24.267601012606615	25.403651061055477	25.806225546242796	24.52252238009511
84-85	24.187714686022208	25.418954802619815	25.70112874295155	24.69220176840643
86-87	23.927920308230618	25.677676324576666	25.741487887897428	24.652915479295284
88-89	23.90517307191063	25.27547589332985	25.916710216009065	24.90264081875045
90-91	24.132912715706944	25.171501267941267	25.935923775326376	24.759662241025417
92-93	24.35603274707954	25.075261620638482	25.884664022190094	24.684041610091885
94-95	24.183562419148092	25.048059754826212	25.94852667496359	24.819851151062107
96-97	24.042912344733764	24.91981195756536	26.056369774619085	24.98090592308179
98-99	23.858347741959008	24.921103113289732	25.977238757053524	25.243310387697736
100-101	24.120196683680064	24.937587850521442	26.015142240696182	24.927073225102315
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	44.0
1	44.0
2	79.5
3	127.5
4	126.0
5	136.5
6	225.0
7	404.5
8	603.5
9	680.0
10	618.5
11	573.0
12	609.5
13	674.5
14	767.0
15	869.5
16	970.5
17	1068.5
18	1175.5
19	1307.5
20	1401.0
21	1492.0
22	1639.0
23	1773.0
24	1913.0
25	2238.5
26	2723.5
27	3488.0
28	4894.0
29	7357.0
30	11192.5
31	16871.0
32	25634.5
33	33157.0
34	39269.5
35	48246.5
36	60747.5
37	73307.0
38	85052.5
39	96551.5
40	106522.5
41	116666.0
42	126640.5
43	138368.0
44	150251.5
45	160891.0
46	170044.0
47	178032.5
48	186628.0
49	193394.5
50	195846.0
51	196005.0
52	195725.5
53	193527.0
54	189100.0
55	180755.0
56	167671.0
57	151334.0
58	136490.5
59	119651.5
60	98877.0
61	81471.5
62	67634.0
63	52886.5
64	38601.5
65	28737.5
66	21937.5
67	17083.0
68	13286.0
69	10278.0
70	8027.5
71	6066.0
72	4482.5
73	3383.0
74	2632.0
75	2109.0
76	1580.0
77	1070.0
78	733.5
79	510.0
80	357.5
81	263.0
82	222.0
83	186.5
84	137.5
85	105.5
86	92.0
87	78.0
88	66.5
89	62.0
90	56.0
91	44.0
92	32.5
93	32.0
94	33.0
95	24.0
96	17.0
97	15.0
98	10.5
99	8.0
100	9.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04916622280493301
2	0.057313499189275356
3	0.04874237605661462
4	0.05493524799037773
5	0.04747083581165946
6	0.016294552768684693
7	0.013327625530455979
8	0.018602162842862587
9	0.01907310367432746
10-11	0.02088622587546723
12-13	0.026419780645179518
14-15	0.042655465809931105
16-17	0.02852724086598484
18-19	0.02225195428671537
20-21	0.02492454350527854
22-23	0.04497484940489562
24-25	0.04035962925653984
26-27	0.029940063360379465
28-29	0.02062720841816155
30-31	0.013080381593936919
32-33	0.009854436898402522
34-35	0.009618966482670084
36-37	0.0014363695359678704
38-39	0.00679332149388083
40-41	0.003626244402279542
42-43	0.20744943626027768
44-45	0.1829251924617443
46-47	0.18902387622921443
48-49	0.18725784811122115
50-51	0.18549181999322786
52-53	0.18995398437135755
54-55	0.18876485877190877
56-57	0.19866638975345774
58-59	0.195358030412417
60-61	0.1998790623944798
62-63	0.1973477554253561
64-65	0.19806594019334006
66-67	0.18892968806292146
68-69	0.18787007119212548
70-71	0.17194049756782606
72-73	0.17903993060215906
74-75	0.17914589228923866
76-77	0.17723858192180592
78-79	0.2032698363810269
80-81	0.21134647164064957
82-83	0.2082618091945546
84-85	0.20372900369170516
86-87	0.21996468885645679
88-89	0.21869314861150158
90-91	0.2175746641367725
92-93	0.22210746963962194
94-95	0.23323344678297964
96-97	0.2331628056582599
98-99	0.22330836875985738
100-101	0.23223269751611675
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4246818.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.08517210940891
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.28614535923734	38.69206666380445
2	13.12406080862264	13.671379319829171
3	4.815797747025353	7.524949634937577
4	2.305585425156529	4.8034725472889015
5	1.3526111243078123	3.5225491603336745
6	0.872258916624771	2.725905347782065
7	0.6108098081870864	2.226989378987761
8	0.4263810950513568	1.7766506175958523
9	0.31955193529285186	1.49795257928606
>10	1.7541330707921763	16.36649078424906
>50	0.09726560254068116	3.421880804414137
>100	0.0333124382140491	2.7993495965404547
>500	0.0014062335968033216	0.4740535098269153
>1k	6.804353504903857E-4	0.4963100551239891
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.709408314648756E-5
2	0.0	0.0	0.0	0.0	7.064112471973133E-5
3	0.0	0.0	0.0	0.0	7.064112471973133E-5
4	0.0	0.0	0.0	0.0	9.418816629297512E-5
5	0.0	0.0	0.0	2.354704157324378E-5	9.418816629297512E-5
6	0.0	0.0	0.0	2.354704157324378E-5	1.1773520786621889E-4
7	0.0	0.0	0.0	2.354704157324378E-5	1.1773520786621889E-4
8	0.0	0.0	0.0	2.354704157324378E-5	1.4128224943946266E-4
9	0.0	0.0	0.0	2.354704157324378E-5	1.6482929101270646E-4
10-11	0.0	0.0	0.0	2.354704157324378E-5	1.8837633258595024E-4
12-13	0.0	0.0	0.0	4.709408314648756E-5	2.3547041573243778E-4
14-15	0.0	0.0	0.0	4.709408314648756E-5	3.532056235986567E-4
16-17	0.0	0.0	0.0	4.709408314648756E-5	4.473937898916318E-4
18-19	0.0	0.0	0.0	7.064112471973133E-5	4.944878730381194E-4
20-21	0.0	0.0	0.0	8.241464550635323E-5	6.004495601177164E-4
22-23	0.0	0.0	0.0	1.05961687079597E-4	6.593171640508259E-4
24-25	0.0	0.0	0.0	2.7079097809230347E-4	7.299582887705571E-4
26-27	0.0	0.0	0.0	4.944878730381194E-4	7.888258927036666E-4
28-29	0.0	0.0	0.0	0.0026843627393497906	8.005994134902885E-4
30-31	0.0	0.0	0.0	0.012056085285500815	8.123729342769104E-4
32-33	0.0	0.0	0.0	0.03242427624635669	8.830140589966417E-4
34-35	0.0	0.0	0.0	0.060515896843236515	9.183346213565074E-4
36-37	0.0	0.0	0.0	0.10437226177340306	9.183346213565074E-4
38-39	0.0	0.0	0.0	0.17852189568754773	9.418816629297511E-4
40-41	0.0	0.0	0.0	0.2631381895809992	9.65428704502995E-4
42-43	0.0	0.0	0.0	0.3526169475593256	9.772022252896168E-4
44-45	0.0	0.0	0.0	0.4602387010698363	0.0010242963084361044
46-47	0.0	0.0	0.0	0.5843433836816176	0.0010831639123692139
48-49	0.0	0.0	0.0	0.7187969910648396	0.0011538050370889451
50-51	0.0	0.0	0.0	0.8638703141975945	0.001177352078662189
52-53	0.0	0.0	0.0	1.0156309971371507	0.0013186343281016517
54-55	0.0	0.0	0.0	1.1905855160263519	0.0014363695359678706
56-57	0.0	0.0	0.0	1.3937376171995126	0.0014599165775411142
58-59	0.0	0.0	0.0	1.6102762115070623	0.001483463619114358
60-61	0.0	0.0	0.0	1.8386471942051672	0.0015070106606876019
62-63	0.0	0.0	0.0	2.0859029042450135	0.0015776517854073331
64-65	0.0	0.0	0.0	2.3544686869086453	0.001589425306193955
66-67	0.0	0.0	0.0	2.6648186948439987	0.0016365193893404426
68-69	0.0	0.0	0.0	3.0061566094897403	0.0016718399517003082
70-71	0.0	0.0	0.0	3.3715478271025505	0.001730707555633418
72-73	0.0	0.0	0.0	3.751844322031224	0.0018955368466461243
74-75	0.0	0.0	0.0	4.1459629303633925	0.00193085740900599
76-77	0.0	0.0	0.0	4.589730475852744	0.00193085740900599
78-79	0.0	0.0	0.0	5.085843094759417	0.0019426309297926117
80-81	0.0	0.0	0.0	5.60144089056795	0.002048592616872209
82-83	0.0	0.0	0.0	6.140526860345793	0.0021663278247384277
84-85	0.0	0.0	0.0	6.704278356171609	0.0021663278247384277
86-87	0.0	0.0	0.0	7.307353411424742	0.0021898748663116713
88-89	0.0	0.0	0.0	7.959535821878875	0.0022134219078849154
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2875	0.0	43.578293	1
GTATCAA	5150	0.0	42.02055	1
TCAACGC	6275	0.0	34.03762	4
CAACGCA	6370	0.0	33.528812	5
ATCAACG	6555	0.0	32.291775	3
GTACATG	13205	0.0	31.94975	1
TATCAAC	6760	0.0	31.59371	2
AACGCAG	6845	0.0	31.190725	6
TACATGG	13860	0.0	29.82564	2
ACATGGG	14150	0.0	28.040098	3
CATGGGG	12390	0.0	25.359875	4
ACGCAGA	8605	0.0	24.208714	7
CGCAGAG	9025	0.0	22.769314	8
ATGGGGG	8425	0.0	21.632483	5
GAGTACA	9280	0.0	21.580853	1
AGTACAT	9685	0.0	19.454754	2
TACACTG	5315	0.0	18.84188	5
GTATAGC	1000	0.0	18.508394	1
GCAGAGT	10380	0.0	18.47112	9
GTAAGTA	2195	0.0	18.162666	2
>>END_MODULE