FastQCFastQC Report
Mon 15 Aug 2016
SRR2921367_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921367_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4246818
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28050.048.9666561
GTATCAA53850.042.2751581
TCAACGC67400.032.7442254
CAACGCA70150.031.3129465
TATCAAC72900.030.7946512
GTACATG133800.030.689731
ATCAACG72350.030.5039483
AACGCAG74700.029.592426
TACATGG138900.029.0099582
ACATGGG141000.027.7027173
ACGCAGA89300.023.9557177
CATGGGG125350.023.891684
CGCAGAG94250.022.2436148
ATGGGGG81700.022.1141955
GAGTACA97900.019.1756381
TACCGTA3054.5256456E-918.7037857
GTACCGT6400.018.569846
AGAGTAC88700.018.08832210-11
GCAGAGT109900.017.7783839
AGTACAT99600.017.5359822
Produced by FastQC (version 0.11.5)