##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921367_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4246818
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.4261548293334	33.0	31.0	34.0	29.0	34.0
2	31.549567699863758	34.0	31.0	34.0	29.0	34.0
3	31.62934154465767	34.0	31.0	34.0	28.0	34.0
4	35.120879915268326	37.0	35.0	37.0	33.0	37.0
5	34.86242664507874	37.0	35.0	37.0	32.0	37.0
6	34.81735925580046	37.0	35.0	37.0	32.0	37.0
7	34.678891113299414	37.0	35.0	37.0	32.0	37.0
8	34.640450096990264	37.0	35.0	37.0	32.0	37.0
9	36.26693774020926	39.0	37.0	39.0	32.0	39.0
10-11	36.11354807293367	39.0	37.0	39.0	32.0	39.0
12-13	36.0373821058496	39.0	37.0	39.0	31.0	39.0
14-15	37.28612127950856	40.0	38.0	41.0	31.0	41.0
16-17	37.25776122263775	40.0	38.0	41.0	31.0	41.0
18-19	37.209945187196624	40.0	38.0	41.0	31.0	41.0
20-21	37.16725122197372	40.0	38.0	41.0	31.0	41.0
22-23	37.06100744133607	40.0	38.0	41.0	31.0	41.0
24-25	36.99181693211247	40.0	37.0	41.0	31.0	41.0
26-27	36.85702318771372	40.0	37.0	41.0	30.0	41.0
28-29	36.703151277026706	40.0	37.0	41.0	30.0	41.0
30-31	36.572071372024894	40.0	37.0	41.0	30.0	41.0
32-33	36.53432205006195	40.0	37.0	41.0	30.0	41.0
34-35	36.67877125885781	40.0	37.0	41.0	30.0	41.0
36-37	36.66493902022644	40.0	37.0	41.0	30.0	41.0
38-39	36.58698536174614	40.0	37.0	41.0	30.0	41.0
40-41	36.4722254403179	40.0	37.0	41.0	30.0	41.0
42-43	36.37718899185225	40.0	36.5	41.0	30.0	41.0
44-45	36.25559878007488	40.0	36.0	41.0	29.0	41.0
46-47	36.11767692893832	40.0	36.0	41.0	28.5	41.0
48-49	35.96878710130738	40.0	35.5	41.0	28.0	41.0
50-51	35.812341381241204	39.5	35.0	41.0	28.0	41.0
52-53	35.63491018451933	39.0	35.0	41.0	28.0	41.0
54-55	35.42172351157973	39.0	35.0	41.0	27.0	41.0
56-57	35.152679723972156	39.0	35.0	41.0	26.0	41.0
58-59	34.92118487300374	38.5	35.0	40.5	26.0	41.0
60-61	34.63599746916397	38.0	34.5	40.0	26.0	41.0
62-63	34.32579451250324	37.0	34.0	40.0	26.0	41.0
64-65	34.00647131570037	37.0	34.0	40.0	26.0	41.0
66-67	33.66553841016969	36.0	34.0	39.0	25.0	41.0
68-69	33.29686037875888	36.0	34.0	39.0	24.0	40.5
70-71	32.91385397255074	35.0	33.0	38.5	23.5	40.0
72-73	32.51966766647405	35.0	33.0	37.0	22.5	39.5
74-75	32.11908857408064	35.0	33.0	37.0	21.5	39.0
76-77	29.37673994976945	33.0	29.0	34.5	16.5	36.5
78-79	31.043997058503567	34.5	31.5	35.5	19.5	37.0
80-81	31.25349544058634	35.0	32.0	35.5	20.0	37.0
82-83	31.102774948208282	35.0	32.0	35.0	19.5	37.0
84-85	30.887924559046326	35.0	32.0	35.0	18.5	36.0
86-87	30.654547122104127	35.0	32.0	35.0	17.5	36.0
88-89	30.396075249751696	35.0	32.0	35.0	10.5	35.5
90-91	30.222385560200603	35.0	32.0	35.0	7.5	35.0
92-93	30.084866128004542	34.0	31.0	35.0	4.5	35.0
94-95	29.994735823385888	34.0	31.0	35.0	2.0	35.0
96-97	29.815653508108895	34.0	31.0	35.0	2.0	35.0
98-99	29.559419782058	34.0	31.0	35.0	2.0	35.0
100-101	28.464637994846967	33.5	29.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	77508.0
3	27108.0
4	14935.0
5	10420.0
6	9229.0
7	9125.0
8	9439.0
9	9943.0
10	11152.0
11	11725.0
12	12739.0
13	13186.0
14	14030.0
15	14965.0
16	15961.0
17	16653.0
18	16234.0
19	15016.0
20	14661.0
21	14710.0
22	15758.0
23	17830.0
24	21149.0
25	25305.0
26	30743.0
27	37308.0
28	44741.0
29	54342.0
30	66026.0
31	82997.0
32	105532.0
33	140715.0
34	206045.0
35	306596.0
36	536398.0
37	1044185.0
38	1050477.0
39	121930.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.274663891535965	18.47256504566302	12.599253723775922	26.653517339025086
2	15.586528404196853	20.225948843388508	37.182072533638255	27.00545021877639
3	18.435708408707818	23.781829776191348	28.490095780739956	29.292366034360874
4	13.35566632569057	15.622851238346058	34.28394548903696	36.73753694692641
5	14.387019261909062	37.333848045698545	33.883422359887106	14.395710332505288
6	35.35127671267972	34.25846867946103	16.231621897698155	14.158632710161095
7	30.170137034556742	31.293803445973122	20.02737582921024	18.508683690259893
8	27.748637840380958	34.863374312915596	18.96052721740795	18.427460629295492
9	27.495456598328445	15.001537621814734	18.275329905825963	39.227675874030865
10-11	26.520329832990463	24.797460632927862	27.095786294922586	21.58642323915909
12-13	26.699986613467463	21.958281348180414	27.134619186115305	24.20711285223682
14-15	23.743476114398636	23.712251182208664	24.023711638928635	28.52056106446406
16-17	24.412929525641264	26.88847173888524	24.847208738235572	23.851389997237927
18-19	24.429565016319334	26.432259749274927	25.98006076571011	23.15811446869563
20-21	24.701262108282904	25.50516473292843	26.046056509127023	23.747516649661637
22-23	24.582133413079465	25.215550024606703	25.776535799812095	24.425780762501738
24-25	24.41231585347303	25.429304358105036	25.697203087346242	24.461176701075694
26-27	24.186974017689515	25.742524131107068	25.767932112886793	24.302569738316627
28-29	24.243115358390387	26.106869941391047	25.44832134336331	24.20169335685525
30-31	24.792325150529383	25.610448541877982	25.43365438260875	24.163571924983884
32-33	24.472673954303	25.644960996771925	25.400188451427123	24.482176597497944
34-35	24.602884998406278	25.74126387030919	25.62601182593362	24.02983930535092
36-37	24.5511517944215	25.748610154996022	25.726581734212445	23.973656316370036
38-39	24.574483703832865	25.825952011291292	25.54760200682324	24.051962278052606
40-41	24.682816692326423	25.548580298865115	25.5396908301657	24.228912178642766
42-43	24.4079303702378	25.611167890431087	25.3811056739025	24.599796065428617
44-45	24.215071392255542	25.63080055453131	25.6523478683113	24.501780184901854
46-47	24.388079662180246	25.513701997206343	25.520460653025097	24.577757687588317
48-49	24.1657925735922	25.70984050607752	25.663859363880043	24.460507556450242
50-51	24.39172601855853	25.561624512988736	25.7096853574595	24.33696411099324
52-53	24.616520870638876	25.423056513773435	25.65083104265657	24.30959157293112
54-55	23.86062030386127	25.322742077014464	26.050000076528683	24.766637542595575
56-57	24.081634911802336	25.437738620679752	25.69855089118414	24.782075576333774
58-59	24.227086837280126	25.473742241078444	25.794755183329322	24.504415738312105
60-61	24.42712471570531	25.501474870680944	25.832667290556827	24.238733123056917
62-63	24.320317753025446	25.464883368483527	25.86696152790878	24.347837350582243
64-65	23.956730856429612	25.266318853360865	25.913916795689246	24.863033494520277
66-67	24.11561573233269	25.494762406160266	25.61831167393358	24.771310187573466
68-69	23.99753208954855	25.423599470240106	25.77245856216306	24.806409878048292
70-71	23.941643891249008	25.21848853518219	25.806617269804953	25.033250303763843
72-73	23.90981645962195	25.583999873755857	25.515625480260635	24.990558186361557
74-75	23.828540991658905	25.583104841990924	25.62907985556026	24.959274310789915
76-77	23.77812641171964	25.1354188744762	25.85845535390174	25.22799935990242
78-79	23.654360346445504	25.184111763607437	25.940610856729833	25.220917033217226
80-81	23.84514213857222	25.421426951699683	25.590703345181897	25.142727564546195
82-83	23.825662922724373	25.455384753071737	25.68375424225795	25.035198081945943
84-85	23.69489939674083	25.45668422040689	25.616090585685015	25.232325797167267
86-87	23.467674040313405	25.66417906490761	25.613924285548485	25.254222609230503
88-89	23.404816638976353	25.29021710582618	25.761953804885103	25.543012450312364
90-91	23.49805338648392	25.152119779681424	25.860421598552126	25.48940523528253
92-93	23.633900878192065	25.068023280400098	25.801728820255633	25.49634702115221
94-95	23.36905400369589	25.054882044078713	25.851494398764192	25.7245695534612
96-97	23.11654997273007	24.95091177636964	25.999732481544523	25.93280576935576
98-99	22.923546421271745	24.87619973815332	25.963369721858548	26.236884118716386
100-101	22.917771677171356	25.019635582890416	25.916273238775634	26.146319501162594
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	54.0
1	44.5
2	67.0
3	103.0
4	91.0
5	104.5
6	175.5
7	330.5
8	523.0
9	556.5
10	443.5
11	332.0
12	349.5
13	409.5
14	415.5
15	442.5
16	488.0
17	554.5
18	614.5
19	676.5
20	759.5
21	861.5
22	974.0
23	1168.0
24	1477.5
25	1842.5
26	2441.5
27	3404.0
28	4976.5
29	7651.0
30	11647.0
31	17443.5
32	26210.5
33	33588.5
34	39721.0
35	48592.0
36	60797.5
37	73123.0
38	84553.5
39	95998.0
40	106286.5
41	116087.5
42	126302.0
43	138599.0
44	150293.5
45	161211.0
46	170544.5
47	179047.5
48	188240.5
49	194958.0
50	197423.0
51	198059.5
52	197740.5
53	195809.5
54	191408.5
55	181878.0
56	168699.0
57	152475.5
58	136702.5
59	119443.5
60	98848.5
61	81506.0
62	67300.0
63	51955.0
64	37496.5
65	27645.0
66	20990.0
67	16244.5
68	12650.0
69	9710.0
70	7355.0
71	5494.5
72	4025.5
73	3086.0
74	2377.5
75	1744.5
76	1212.5
77	746.0
78	441.0
79	276.5
80	178.0
81	107.5
82	71.5
83	45.5
84	31.0
85	24.0
86	18.5
87	18.0
88	12.0
89	3.5
90	2.0
91	4.0
92	3.5
93	2.5
94	2.0
95	1.0
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14827572078671608
2	3.7675266517190047E-4
3	0.005745478143871482
4	0.0043797497326233436
5	0.025454351940676523
6	0.0012244461618086764
7	1.412822494394627E-4
8	0.0016247458685538206
9	0.0
10-11	0.09856791602559846
12-13	2.9433801966554724E-4
14-15	0.004980199292741059
16-17	2.5901745730568155E-4
18-19	3.532056235986567E-4
20-21	8.241464550635323E-4
22-23	1.8837633258595024E-4
24-25	0.0015070106606876019
26-27	0.002849192030362497
28-29	0.006652039244441367
30-31	0.014999465482156289
32-33	0.014858183232716824
34-35	0.011125977143357686
36-37	7.41731809557179E-4
38-39	1.5305577022608455E-4
40-41	0.0051803491461136315
42-43	0.008429840883221273
44-45	0.008217917509062079
46-47	0.009689607607389815
48-49	0.012056085285500815
50-51	0.005957401518030676
52-53	0.0017542545972066615
54-55	0.0010478433500093482
56-57	0.006522530515788528
58-59	0.009795569294469411
60-61	0.008100182301195859
62-63	0.017730922304652563
64-65	0.03383709874075131
66-67	0.03645082035538137
68-69	0.026396233603606278
70-71	0.041066040503737154
72-73	0.02534839025359693
74-75	0.024335867465947448
76-77	0.23462272223580102
78-79	0.06735631242026383
80-81	0.05369902830778244
82-83	0.019967891254110724
84-85	0.032471370329503174
86-87	0.08101359653274522
88-89	0.2585582899950033
90-91	0.31345821742302116
92-93	0.05019051911336912
94-95	0.0049566522511678155
96-97	0.008994969880979123
98-99	0.0013657284112481391
100-101	0.0014952371399009799
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4246818.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.35986537745932
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.50667958556329	37.75293167858568
2	13.617731865259486	13.988097506921305
3	4.908892705558588	7.563602055296435
4	2.363054205958552	4.85464583590681
5	1.3746744073099544	3.5301546248638975
6	0.8750647011296204	2.6965923147950392
7	0.6197943616352349	2.228278848270581
8	0.437459502391958	1.7974288920753048
9	0.3362718983193679	1.5543791485114884
>10	1.826358201028488	16.84942141614297
>50	0.09789323151057427	3.4050182300168332
>100	0.03405839890435821	2.8294811953519914
>500	0.0013320251644032186	0.4457200822447015
>1k	7.349102661395655E-4	0.5042481710170347
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.709408314648756E-5
2	0.0	0.0	0.0	0.0	4.709408314648756E-5
3	0.0	0.0	0.0	0.0	4.709408314648756E-5
4	0.0	0.0	0.0	0.0	4.709408314648756E-5
5	0.0	0.0	0.0	0.0	4.709408314648756E-5
6	0.0	0.0	0.0	0.0	7.064112471973133E-5
7	0.0	0.0	0.0	0.0	7.064112471973133E-5
8	0.0	0.0	0.0	2.354704157324378E-5	1.1773520786621889E-4
9	0.0	0.0	0.0	9.418816629297512E-5	1.4128224943946266E-4
10-11	0.0	0.0	0.0	9.418816629297512E-5	1.5305577022608455E-4
12-13	0.0	0.0	0.0	1.1773520786621889E-4	2.9433801966554724E-4
14-15	0.0	1.177352078662189E-5	0.0	1.1773520786621889E-4	3.532056235986567E-4
16-17	0.0	2.354704157324378E-5	0.0	1.1773520786621889E-4	4.0029970674514424E-4
18-19	0.0	2.354704157324378E-5	0.0	1.1773520786621889E-4	4.0029970674514424E-4
20-21	0.0	2.354704157324378E-5	0.0	1.2950872865284078E-4	4.7094083146487556E-4
22-23	0.0	2.354704157324378E-5	0.0	1.5305577022608455E-4	5.533554769712288E-4
24-25	0.0	3.532056235986567E-5	0.0	2.9433801966554724E-4	6.122230809043383E-4
26-27	0.0	4.709408314648756E-5	0.0	5.29808435397985E-4	6.122230809043383E-4
28-29	0.0	4.709408314648756E-5	0.0	0.002778550905642766	6.122230809043383E-4
30-31	0.0	4.709408314648756E-5	0.0	0.012350423305166362	6.122230809043383E-4
32-33	0.0	4.709408314648756E-5	0.0	0.03303649932726102	6.593171640508259E-4
34-35	2.354704157324378E-5	4.709408314648756E-5	0.0	0.061493099068526125	6.828642056240696E-4
36-37	2.354704157324378E-5	4.709408314648756E-5	0.0	0.10538478456105253	6.828642056240696E-4
38-39	2.354704157324378E-5	4.709408314648756E-5	0.0	0.178886874831933	7.41731809557179E-4
40-41	2.354704157324378E-5	5.8867603933109445E-5	0.0	0.2632206042265056	7.770523719170447E-4
42-43	2.354704157324378E-5	7.064112471973133E-5	0.0	0.3527346827671918	7.770523719170447E-4
44-45	2.354704157324378E-5	7.064112471973133E-5	0.0	0.46027402163219616	7.888258927036666E-4
46-47	2.354704157324378E-5	7.064112471973133E-5	0.0	0.5845553070557767	8.47693496636776E-4
48-49	2.354704157324378E-5	7.064112471973133E-5	0.0	0.7177726947564035	9.183346213565074E-4
50-51	2.354704157324378E-5	7.064112471973133E-5	0.0	0.8618688156638687	9.418816629297511E-4
52-53	2.354704157324378E-5	7.064112471973133E-5	0.0	1.0132409724174665	0.0010831639123692139
54-55	4.709408314648756E-5	7.064112471973133E-5	0.0	1.1875832682257634	0.001165578557875567
56-57	4.709408314648756E-5	7.064112471973133E-5	0.0	1.3901584668803797	0.0012008991202354328
58-59	4.709408314648756E-5	7.064112471973133E-5	0.0	1.6065440054177034	0.0012362196825952984
60-61	4.709408314648756E-5	7.064112471973133E-5	0.0	1.8340790681399581	0.001271540244955164
62-63	4.709408314648756E-5	7.064112471973133E-5	0.0	2.07978067343597	0.0014128224943946268
64-65	4.709408314648756E-5	7.064112471973133E-5	0.0	2.3473103862703795	0.0014128224943946268
66-67	4.709408314648756E-5	7.064112471973133E-5	0.0	2.6569186623961754	0.0014128224943946268
68-69	4.709408314648756E-5	7.064112471973133E-5	0.0	2.9974559776284266	0.0014128224943946268
70-71	4.709408314648756E-5	7.064112471973133E-5	0.0	3.363223947906409	0.0014245960151812488
72-73	4.709408314648756E-5	7.064112471973133E-5	0.0	3.743850101417108	0.0015305577022608457
74-75	4.709408314648756E-5	7.064112471973133E-5	0.0	4.140311640385813	0.0015658782646207113
76-77	4.709408314648756E-5	7.064112471973133E-5	0.0	4.586445663553277	0.0016247458685538208
78-79	4.709408314648756E-5	7.064112471973133E-5	0.0	5.085466342094246	0.0016482929101270646
80-81	4.709408314648756E-5	7.064112471973133E-5	0.0	5.606067884237092	0.0016836134724869303
82-83	4.709408314648756E-5	7.064112471973133E-5	0.0	6.1485681750430565	0.001718934034846796
84-85	4.709408314648756E-5	7.064112471973133E-5	0.0	6.717735490430718	0.001718934034846796
86-87	4.709408314648756E-5	7.064112471973133E-5	0.0	7.32646183566143	0.0017660281179932833
88-89	4.709408314648756E-5	7.064112471973133E-5	0.0	7.984036518635835	0.001813122201139771
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2805	0.0	48.966656	1
GTATCAA	5385	0.0	42.275158	1
TCAACGC	6740	0.0	32.744225	4
CAACGCA	7015	0.0	31.312946	5
TATCAAC	7290	0.0	30.794651	2
GTACATG	13380	0.0	30.68973	1
ATCAACG	7235	0.0	30.503948	3
AACGCAG	7470	0.0	29.59242	6
TACATGG	13890	0.0	29.009958	2
ACATGGG	14100	0.0	27.702717	3
ACGCAGA	8930	0.0	23.955717	7
CATGGGG	12535	0.0	23.89168	4
CGCAGAG	9425	0.0	22.243614	8
ATGGGGG	8170	0.0	22.114195	5
GAGTACA	9790	0.0	19.175638	1
TACCGTA	305	4.5256456E-9	18.703785	7
GTACCGT	640	0.0	18.56984	6
AGAGTAC	8870	0.0	18.088322	10-11
GCAGAGT	10990	0.0	17.778383	9
AGTACAT	9960	0.0	17.535982	2
>>END_MODULE