FastQCFastQC Report
Mon 15 Aug 2016
SRR2921366_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921366_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2951131
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29950.057.0379181
GTATCAA53900.041.641881
TCAACGC66050.032.764354
ATCAACG67600.032.012013
CAACGCA68000.031.7517625
TATCAAC71350.030.528552
AACGCAG72100.029.8722656
GTACATG93250.025.494521
ACGCAGA86150.024.8390547
TACATGG97600.023.970912
CGCAGAG88350.023.6855058
ACATGGG97900.023.5100673
CATGGGG83500.021.3703164
GCAGAGT101350.019.5705559
GTATTAG7800.018.2509161
CAGAGTA94350.018.15597510-11
ATGGGGG57200.018.0853045
GAGTACT41500.017.66600812-13
AGAGTAC86550.017.51693510-11
GAGTACA71950.016.487971
Produced by FastQC (version 0.11.5)