##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921366_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2951131
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.595370385116755	31.0	31.0	34.0	27.0	34.0
2	30.655865835843954	33.0	31.0	34.0	27.0	34.0
3	30.691701588306312	33.0	31.0	34.0	27.0	34.0
4	34.17259213501536	37.0	35.0	37.0	30.0	37.0
5	34.172696840635	37.0	35.0	37.0	30.0	37.0
6	34.220516134322736	37.0	35.0	37.0	30.0	37.0
7	34.149651777572736	37.0	35.0	37.0	30.0	37.0
8	34.1162649167387	37.0	35.0	37.0	30.0	37.0
9	35.69132410591058	39.0	37.0	39.0	30.0	39.0
10-11	35.59051478907578	39.0	37.0	39.0	30.0	39.0
12-13	35.487023449653705	39.0	37.0	39.0	30.0	39.0
14-15	36.636803313712605	40.0	37.0	41.0	30.0	41.0
16-17	36.583192172763596	40.0	37.0	41.0	30.0	41.0
18-19	36.54633986088723	40.0	37.0	41.0	29.5	41.0
20-21	36.49274396832943	40.0	37.0	41.0	29.0	41.0
22-23	36.38180243438872	40.0	37.0	41.0	29.0	41.0
24-25	36.28330104627683	40.0	36.5	41.0	28.0	41.0
26-27	36.13435154183261	40.0	36.0	41.0	27.5	41.0
28-29	35.99882011337348	40.0	36.0	41.0	27.0	41.0
30-31	35.84592991636088	40.0	36.0	41.0	27.0	41.0
32-33	35.66163684363724	40.0	35.5	41.0	26.5	41.0
34-35	35.500673809464914	39.5	35.0	41.0	25.5	41.0
36-37	35.33021983775034	39.0	35.0	41.0	25.0	41.0
38-39	35.5453715880454	39.5	35.0	41.0	26.0	41.0
40-41	35.662555643920925	40.0	35.5	41.0	26.0	41.0
42-43	35.49234191908119	40.0	35.0	41.0	25.0	41.0
44-45	35.37105028546682	40.0	35.0	41.0	24.5	41.0
46-47	35.2161981287852	40.0	35.0	41.0	24.0	41.0
48-49	35.06835396327713	40.0	35.0	41.0	23.5	41.0
50-51	33.363163648106436	37.0	32.5	39.5	20.0	40.0
52-53	33.52471611731231	37.5	33.5	39.5	21.0	40.0
54-55	34.39314825400838	39.0	34.0	40.5	22.0	41.0
56-57	34.41298759695859	39.0	34.0	41.0	22.0	41.0
58-59	34.24297870884078	38.5	34.0	41.0	21.0	41.0
60-61	34.00559412645525	38.0	34.0	40.5	20.0	41.0
62-63	33.72350752982501	37.5	34.0	40.0	18.5	41.0
64-65	33.44321295801508	37.0	34.0	40.0	18.0	41.0
66-67	33.11172411526293	36.5	33.5	39.5	15.5	41.0
68-69	32.753157179400034	36.0	33.0	39.0	12.5	41.0
70-71	32.375167859373235	35.5	33.0	39.0	9.5	41.0
72-73	32.02432965530842	35.0	33.0	38.5	7.5	40.0
74-75	31.624262697928355	35.0	32.0	37.0	4.0	39.5
76-77	31.24316829717149	35.0	32.0	37.0	2.0	39.0
78-79	30.8950232978475	35.0	32.0	36.0	2.0	39.0
80-81	30.53812318057043	35.0	31.5	36.0	2.0	37.5
82-83	30.22696196814035	35.0	31.0	35.5	2.0	37.0
84-85	29.983361972071044	35.0	31.0	35.0	2.0	37.0
86-87	29.747689106312123	35.0	31.0	35.0	2.0	36.0
88-89	29.533874470499615	35.0	31.0	35.0	2.0	36.0
90-91	29.272186155070717	35.0	31.0	35.0	2.0	36.0
92-93	29.072816659104593	35.0	31.0	35.0	2.0	35.0
94-95	28.86019800544266	34.0	30.0	35.0	2.0	35.0
96-97	28.60743643708124	34.0	30.0	35.0	2.0	35.0
98-99	28.375450971170036	34.0	30.0	35.0	2.0	35.0
100-101	27.3284864345229	33.5	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	89347.0
3	15780.0
4	10891.0
5	8307.0
6	7716.0
7	7972.0
8	8339.0
9	9124.0
10	9831.0
11	10528.0
12	10949.0
13	11176.0
14	12332.0
15	13488.0
16	13784.0
17	14717.0
18	13141.0
19	12068.0
20	12008.0
21	12336.0
22	13669.0
23	15736.0
24	18456.0
25	21407.0
26	25560.0
27	30324.0
28	36000.0
29	42548.0
30	51126.0
31	62418.0
32	77675.0
33	100979.0
34	144152.0
35	207699.0
36	337160.0
37	620764.0
38	699453.0
39	142150.0
40	21.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.091322747340705	18.798663071782087	13.84623833840665	24.263775842470558
2	15.977452594812917	20.036148266886904	39.301560695150634	24.68483844314954
3	18.859203059211197	24.160106855426505	30.076785387384824	26.903904697977477
4	13.641837477106803	16.436960556230986	36.733336362027494	33.187865604634716
5	14.259678560495601	37.368249229691706	34.31827250251119	14.053799707301495
6	32.74211335779371	35.32363684673943	17.51695066328388	14.417299132182976
7	28.129901379333717	32.37638526451351	21.429084623987528	18.06462873216525
8	26.51902393658665	34.29121254167873	20.473600479368905	18.716163042365718
9	26.885953302737775	15.619427921086906	20.072752858208524	37.4218659179668
10-11	25.391561283255427	25.479732899146452	28.06792485060139	21.060780966996738
12-13	25.8034880548273	23.19100582811221	27.780165437718512	23.225340679341976
14-15	23.354703197987845	24.879227749150342	25.20246227758134	26.563606775280473
16-17	23.254566540289325	27.717023894079617	26.192513519426807	22.835896046204248
18-19	23.216957517237592	27.162433054828313	27.520326946127472	22.100282481806623
20-21	23.734850756019576	26.286917821881346	27.489299163642595	22.488932258456487
22-23	23.82647003236652	25.96164325977204	27.08571661032118	23.126170097540264
24-25	23.591659228669844	26.211235267935685	26.965741371951935	23.23136413144254
26-27	23.21726882358614	26.463392912071775	27.082545615333526	23.236792649008564
28-29	23.27230590411951	26.78584410375188	26.887146958787632	23.054703033340974
30-31	23.93457217675564	26.21649044670819	26.726375517112018	23.122561859424156
32-33	23.542080107142084	26.405951780640684	26.57342906174767	23.47853905046956
34-35	23.894672614544284	26.49290129831796	26.643230533236707	22.969195553901045
36-37	23.8525031374038	26.320712727329408	26.766633238300535	23.060150896966253
38-39	23.718802642284757	26.443027641107165	26.634018213235077	23.204151503373
40-41	23.857162680786974	26.236203294350783	26.66829096297672	23.23834306188552
42-43	23.708504815071375	26.320867799464843	26.390920636214226	23.579706749249553
44-45	23.55752191359492	26.32990569176348	26.655112945825383	23.457459448816227
46-47	23.740498794509488	26.21946530643632	26.37190218305564	23.668133715998554
48-49	23.461474936909205	26.36892213746367	26.526990740119906	23.642612185507218
50-51	23.626074238841056	26.34949928265255	26.606936373012523	23.417490105493872
52-53	23.8312125811398	26.232249568106	26.504227782688357	23.43231006806585
54-55	23.194484076348786	26.131820702605342	26.843157981041305	23.83053724000457
56-57	23.381648302247598	26.24840926962897	26.56514626570528	23.804796162418153
58-59	23.48976551724138	26.148838196286473	26.704246153846157	23.657150132625997
60-61	23.562811528066387	26.21149614961496	26.87134145513317	23.354350867185484
62-63	23.616037391992712	26.12183532726397	26.860317676432345	23.401809604310973
64-65	23.244973311700427	25.968841891963702	26.84516474153153	23.941020054804344
66-67	23.300411188719547	26.064082156942263	26.70312812713834	23.932378527199855
68-69	23.312773463917022	26.044713642030132	26.845991776052237	23.796521118000612
70-71	23.273569649883637	25.900528582562345	26.73744531553378	24.08845645202024
72-73	23.22255269535893	26.16379755897978	26.620094905367058	23.99355484029423
74-75	23.22196103884862	26.277382174265867	26.670979049525243	23.82967773736027
76-77	23.239731377401988	25.908576933665508	26.68984735439058	24.16184433454193
78-79	23.186025266150924	26.014159516197573	26.71062462851261	24.08919058913889
80-81	23.431838070274274	26.127916251957238	26.518243988708356	23.922001689060128
82-83	23.349277406153128	26.176763919172174	26.70544474353258	23.768513931142117
84-85	23.39314646166164	26.21034701303058	26.517677982270833	23.87882854303695
86-87	23.13282075551315	26.454541024140198	26.497111506986602	23.915526713360048
88-89	23.086806903364913	26.13187968591924	26.666605537675323	24.11470787304052
90-91	23.242312363808573	25.963126922047014	26.773759349483605	24.02080136466081
92-93	23.43775391736386	25.86423812776863	26.73967090696459	23.95833704790292
94-95	23.29891430703497	25.856064377012565	26.772472862770712	24.072548453181753
96-97	23.171839115694375	25.678003478434537	26.903811146241154	24.246346259629934
98-99	22.897566908199067	25.666305593531725	26.845841356158772	24.59028614211044
100-101	23.169961474482044	25.716908346289806	26.88897738822972	24.224152790998428
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	157.0
1	126.0
2	124.0
3	154.0
4	146.5
5	160.5
6	253.5
7	488.0
8	737.5
9	863.5
10	773.0
11	615.5
12	644.5
13	728.5
14	796.5
15	900.0
16	1049.5
17	1120.5
18	1222.5
19	1410.0
20	1534.0
21	1741.0
22	1930.5
23	2118.0
24	2523.5
25	3102.5
26	4131.0
27	5788.0
28	8243.0
29	12180.0
30	17795.0
31	25133.5
32	34326.5
33	41823.5
34	48056.5
35	55288.5
36	63949.5
37	72468.5
38	78834.0
39	84794.0
40	89427.5
41	92856.5
42	96722.0
43	101449.0
44	106145.0
45	112255.0
46	116410.5
47	118695.5
48	121397.5
49	122362.5
50	122369.0
51	120383.5
52	117811.0
53	116243.0
54	113376.0
55	107624.5
56	99758.5
57	90117.5
58	80899.5
59	69798.0
60	56495.5
61	46125.0
62	37961.5
63	29330.0
64	21227.0
65	15664.5
66	11956.0
67	9297.5
68	7273.5
69	5572.0
70	4120.5
71	3031.0
72	2248.0
73	1679.5
74	1279.0
75	969.5
76	727.0
77	493.5
78	325.5
79	219.5
80	165.5
81	125.0
82	86.5
83	72.0
84	64.5
85	49.5
86	44.5
87	44.0
88	39.0
89	32.0
90	26.5
91	24.0
92	19.0
93	16.5
94	16.5
95	13.5
96	8.5
97	8.0
98	9.0
99	7.0
100	6.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04689727429924324
2	0.05462312584565036
3	0.04577905894384221
4	0.05303051609704889
5	0.0453385498644418
6	0.017247624724215902
7	0.014604570247813467
8	0.019822908573018275
9	0.02012787639721856
10-11	0.02151717426301984
12-13	0.026295003508824244
14-15	0.04271243804493938
16-17	0.028446720935126228
18-19	0.022889529471921105
20-21	0.026345831479524292
22-23	0.04484721281434135
24-25	0.04025575279443712
26-27	0.030140986625127788
28-29	0.019399342150517886
30-31	0.012469795478411497
32-33	0.009589543805408843
34-35	0.009301518638108576
36-37	0.0015078964641013902
38-39	0.0067770627600062475
40-41	0.0037104418611034213
42-43	0.21046168401199403
44-45	0.18538655179997093
46-47	0.1914350803132765
48-49	0.18889368177827415
50-51	0.1883006887867736
52-53	0.1922822131582773
54-55	0.19052017684067565
56-57	0.20138041991358568
58-59	0.19716169834548178
60-61	0.20232920869998655
62-63	0.19997417939088438
64-65	0.20082131223588515
66-67	0.19133342437187642
68-69	0.18931724820077456
70-71	0.17413662761836055
72-73	0.18143891274226728
74-75	0.18130337148706716
76-77	0.17918553937456522
78-79	0.20481977926428882
80-81	0.21340970631259676
82-83	0.21191875250539538
84-85	0.2066157008956905
86-87	0.22372778436470625
88-89	0.22154218162460426
90-91	0.22081364737790357
92-93	0.2251509675443076
94-95	0.23633312109831792
96-97	0.2368752861191184
98-99	0.22593032976170832
100-101	0.23646866235351802
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2951131.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.39494266336403
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.3305943486848	44.573206804519465
2	13.023174550446386	15.209750623365967
3	4.44650142902834	7.789595880020282
4	2.061290722729617	4.814758142652807
5	1.1533877267186603	3.367600508518197
6	0.7067317030658058	2.47617343673456
7	0.44419237839158693	1.815701192737601
8	0.3260056466968924	1.5229664837438353
9	0.2446880373633668	1.2859689521020392
>10	1.1921449941394535	12.066556942941013
>50	0.05063686862444691	2.0352355147163324
>100	0.01867457702910354	1.9483120242151173
>500	0.0012792463472219358	0.5322143540206947
>1k	6.977707345035669E-4	0.5619591397121476
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.388531380003124E-5	0.0
5	0.0	0.0	0.0	6.777062760006248E-5	0.0
6	0.0	0.0	0.0	6.777062760006248E-5	6.777062760006248E-5
7	0.0	0.0	0.0	6.777062760006248E-5	6.777062760006248E-5
8	0.0	0.0	0.0	6.777062760006248E-5	6.777062760006248E-5
9	0.0	0.0	0.0	6.777062760006248E-5	1.0165594140009373E-4
10-11	3.388531380003124E-5	0.0	0.0	6.777062760006248E-5	1.0165594140009373E-4
12-13	3.388531380003124E-5	0.0	0.0	1.3554125520012496E-4	3.0496782420028117E-4
14-15	5.082797070004686E-5	0.0	0.0	1.3554125520012496E-4	4.066237656003749E-4
16-17	6.777062760006248E-5	0.0	0.0	1.5248391210014058E-4	4.743943932004374E-4
18-19	6.777062760006248E-5	0.0	0.0	1.694265690001562E-4	5.082797070004686E-4
20-21	6.777062760006248E-5	3.388531380003124E-5	0.0	1.694265690001562E-4	5.591076777005155E-4
22-23	6.777062760006248E-5	3.388531380003124E-5	0.0	2.8802516730026557E-4	5.929929915005467E-4
24-25	6.777062760006248E-5	3.388531380003124E-5	0.0	4.066237656003749E-4	6.099356484005623E-4
26-27	6.777062760006248E-5	3.388531380003124E-5	0.0	8.47132845000781E-4	6.607636191006093E-4
28-29	6.777062760006248E-5	3.388531380003124E-5	0.0	0.0024058572798022182	6.777062760006248E-4
30-31	6.777062760006248E-5	3.388531380003124E-5	0.0	0.00913209206910842	6.777062760006248E-4
32-33	1.0165594140009373E-4	3.388531380003124E-5	0.0	0.0266846846175246	6.777062760006248E-4
34-35	1.0165594140009373E-4	3.388531380003124E-5	0.0	0.05201395668304795	7.11591589800656E-4
36-37	1.0165594140009373E-4	3.388531380003124E-5	0.0	0.0928288171551856	7.11591589800656E-4
38-39	1.0165594140009373E-4	3.388531380003124E-5	0.0	0.15592327145084378	7.11591589800656E-4
40-41	1.0165594140009373E-4	3.388531380003124E-5	0.0	0.22540510739780784	7.11591589800656E-4
42-43	1.0165594140009373E-4	3.388531380003124E-5	0.0	0.30112184108397766	7.11591589800656E-4
44-45	1.0165594140009373E-4	3.388531380003124E-5	0.0	0.40669153622797494	7.454769036006872E-4
46-47	1.0165594140009373E-4	6.777062760006248E-5	0.0	0.5317283441500903	7.793622174007185E-4
48-49	1.0165594140009373E-4	6.777062760006248E-5	0.0	0.6591879520089078	7.963048743007342E-4
50-51	1.0165594140009373E-4	6.777062760006248E-5	0.0	0.7868169864367254	8.132475312007499E-4
52-53	1.0165594140009373E-4	6.777062760006248E-5	0.0	0.9225784961765506	8.640755019007967E-4
54-55	1.0165594140009373E-4	8.47132845000781E-5	0.0	1.0873119492154024	8.810181588008122E-4
56-57	1.0165594140009373E-4	1.0165594140009373E-4	0.0	1.2806446071014808	8.979608157008279E-4
58-59	1.0165594140009373E-4	1.0165594140009373E-4	0.0	1.4821097402995664	9.318461295008591E-4
60-61	1.0165594140009373E-4	1.0165594140009373E-4	0.0	1.697518680126365	0.0010165594140009373
62-63	1.0165594140009373E-4	1.0165594140009373E-4	0.0	1.923906461624374	0.0010504447278009686
64-65	1.0165594140009373E-4	1.0165594140009373E-4	0.0	2.1816889863581115	0.0010504447278009686
66-67	1.0165594140009373E-4	1.0165594140009373E-4	0.0	2.487978337796594	0.0010504447278009686
68-69	1.0165594140009373E-4	1.3554125520012496E-4	0.0	2.823612370985903	0.0010504447278009686
70-71	1.0165594140009373E-4	1.3554125520012496E-4	0.0	3.1656676711403184	0.0011012726985010152
72-73	1.0165594140009373E-4	1.3554125520012496E-4	0.0	3.526326008570951	0.0011351580123010467
74-75	1.0165594140009373E-4	1.3554125520012496E-4	0.0	3.9208866024585154	0.0011521006692010623
76-77	1.0165594140009373E-4	1.3554125520012496E-4	0.0	4.373001401835432	0.0011521006692010623
78-79	1.0165594140009373E-4	1.3554125520012496E-4	0.0	4.876249139736595	0.0011521006692010623
80-81	1.0165594140009373E-4	1.5248391210014058E-4	0.0	5.399777237947079	0.0011521006692010623
82-83	1.0165594140009373E-4	1.694265690001562E-4	0.0	5.941213724500878	0.0012198712968011247
84-85	1.0165594140009373E-4	1.694265690001562E-4	0.0	6.513096165504005	0.0012368139537011404
86-87	1.0165594140009373E-4	1.694265690001562E-4	0.0	7.144108479088187	0.001253756610601156
88-89	1.0165594140009373E-4	1.694265690001562E-4	0.0	7.829083154898919	0.001253756610601156
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2995	0.0	57.037918	1
GTATCAA	5390	0.0	41.64188	1
TCAACGC	6605	0.0	32.76435	4
ATCAACG	6760	0.0	32.01201	3
CAACGCA	6800	0.0	31.751762	5
TATCAAC	7135	0.0	30.52855	2
AACGCAG	7210	0.0	29.872265	6
GTACATG	9325	0.0	25.49452	1
ACGCAGA	8615	0.0	24.839054	7
TACATGG	9760	0.0	23.97091	2
CGCAGAG	8835	0.0	23.685505	8
ACATGGG	9790	0.0	23.510067	3
CATGGGG	8350	0.0	21.370316	4
GCAGAGT	10135	0.0	19.570555	9
GTATTAG	780	0.0	18.250916	1
CAGAGTA	9435	0.0	18.155975	10-11
ATGGGGG	5720	0.0	18.085304	5
GAGTACT	4150	0.0	17.666008	12-13
AGAGTAC	8655	0.0	17.516935	10-11
GAGTACA	7195	0.0	16.48797	1
>>END_MODULE