FastQCFastQC Report
Mon 15 Aug 2016
SRR2921366_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921366_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2951131
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31800.056.939841
GTATCAA59200.042.6276321
TCAACGC74850.033.1624344
CAACGCA76500.032.5666125
ATCAACG76250.032.5535473
TATCAAC77500.032.0897332
AACGCAG81100.030.8324896
ACGCAGA95250.025.8029467
GTACATG89500.025.7004491
CGCAGAG96650.024.937328
TACATGG90850.023.9263822
ACATGGG94000.022.4177283
GCAGAGT106350.020.9642379
CATGGGG79850.020.5654264
GAGTACT46800.019.41387412-13
TAAGGTG14550.019.2793225
AGAGTAC90550.018.97857310-11
CAGAGTA98100.018.97170810-11
ATGGGGG56300.018.8321195
GTACTTT48950.018.36732314-15
Produced by FastQC (version 0.11.5)