##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921366_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2951131
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.42577777807898	33.0	31.0	34.0	29.0	34.0
2	31.55241871675639	34.0	31.0	34.0	29.0	34.0
3	31.62058275284967	34.0	31.0	34.0	28.0	34.0
4	35.11944776426394	37.0	35.0	37.0	33.0	37.0
5	34.858138456069895	37.0	35.0	37.0	32.0	37.0
6	34.81863224641671	37.0	35.0	37.0	32.0	37.0
7	34.689124271338684	37.0	35.0	37.0	32.0	37.0
8	34.65330071758929	37.0	35.0	37.0	32.0	37.0
9	36.2685763525916	39.0	37.0	39.0	32.0	39.0
10-11	36.12338286575553	39.0	37.0	39.0	32.0	39.0
12-13	36.05664082685587	39.0	37.0	39.0	31.5	39.0
14-15	37.285104761530405	40.0	38.0	41.0	31.0	41.0
16-17	37.26537012419984	40.0	38.0	41.0	31.0	41.0
18-19	37.23985160265674	40.0	38.0	41.0	31.0	41.0
20-21	37.205131185298114	40.0	38.0	41.0	31.0	41.0
22-23	37.097829442339226	40.0	38.0	41.0	31.0	41.0
24-25	37.02447400674521	40.0	37.5	41.0	31.0	41.0
26-27	36.88171653511823	40.0	37.0	41.0	30.0	41.0
28-29	36.7270185566144	40.0	37.0	41.0	30.0	41.0
30-31	36.58178474625491	40.0	37.0	41.0	30.0	41.0
32-33	36.5160628247272	40.0	36.5	41.0	30.0	41.0
34-35	36.63387782514568	40.0	37.0	41.0	30.0	41.0
36-37	36.6030359208046	40.0	37.0	41.0	30.0	41.0
38-39	36.51057577586356	40.0	37.0	41.0	30.0	41.0
40-41	36.38160640784838	40.0	36.5	41.0	29.5	41.0
42-43	36.27153572647232	40.0	36.0	41.0	29.0	41.0
44-45	36.125425133618265	40.0	36.0	41.0	28.5	41.0
46-47	35.96001770168793	40.0	36.0	41.0	28.0	41.0
48-49	35.798004561640944	40.0	35.5	41.0	27.5	41.0
50-51	35.646854036638835	40.0	35.0	41.0	27.0	41.0
52-53	35.47924829497572	39.0	35.0	41.0	26.5	41.0
54-55	35.277131547193264	39.0	35.0	41.0	26.0	41.0
56-57	35.024967546340704	39.0	35.0	41.0	26.0	41.0
58-59	34.806798817131465	38.5	35.0	40.5	26.0	41.0
60-61	34.53779635671883	38.0	34.5	40.0	25.0	41.0
62-63	34.24418756741059	37.5	34.0	40.0	24.0	41.0
64-65	33.93587695700394	37.0	34.0	40.0	23.5	41.0
66-67	33.609598828381394	36.5	34.0	39.5	23.0	41.0
68-69	33.25282086766057	36.0	34.0	39.0	22.0	41.0
70-71	32.87448557857987	35.5	33.0	39.0	22.0	40.0
72-73	32.481531317993	35.0	33.0	38.0	20.5	40.0
74-75	32.07256184154482	35.0	33.0	37.0	19.5	39.0
76-77	29.306714103846964	33.0	29.0	34.5	12.0	36.5
78-79	30.964103931679077	34.5	31.5	36.0	15.0	38.0
80-81	31.156062370663996	35.0	32.0	36.0	17.0	37.0
82-83	30.986105327076295	35.0	32.0	35.0	14.5	37.0
84-85	30.75090058692752	35.0	32.0	35.0	11.0	36.5
86-87	30.501199201255382	35.0	32.0	35.0	7.0	36.0
88-89	30.216785530699923	35.0	32.0	35.0	2.0	36.0
90-91	30.027594674719623	34.5	31.0	35.0	2.0	35.5
92-93	29.878921166156296	34.0	31.0	35.0	2.0	35.0
94-95	29.777767913386427	34.0	31.0	35.0	2.0	35.0
96-97	29.587915954933887	34.0	31.0	35.0	2.0	35.0
98-99	29.323809583512222	34.0	31.0	35.0	2.0	35.0
100-101	28.237594501904525	33.0	28.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	53512.0
3	18738.0
4	10249.0
5	7063.0
6	6353.0
7	6495.0
8	6583.0
9	7275.0
10	8018.0
11	8654.0
12	9423.0
13	10291.0
14	11359.0
15	12886.0
16	13762.0
17	15133.0
18	13520.0
19	11897.0
20	11186.0
21	10894.0
22	11390.0
23	12720.0
24	15124.0
25	17955.0
26	21767.0
27	26081.0
28	30714.0
29	37624.0
30	45314.0
31	56460.0
32	72464.0
33	94774.0
34	139248.0
35	205379.0
36	350870.0
37	687283.0
38	764671.0
39	107997.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.94477447938901	20.48071382565513	14.538964974518345	25.035546720437512
2	14.953881916018325	21.44185936186939	37.98774702485836	25.616511697253923
3	17.499214662613298	25.336119318418643	29.995448940192702	27.169217078775354
4	13.222134530400126	17.643259501638603	35.83474161730754	33.29986435065373
5	13.998243605956285	37.7067499829682	34.332995634774434	13.962010776301087
6	32.56502938406805	36.06160302179975	17.2802650140338	14.0931025800984
7	27.796441087232466	32.61219615834769	21.470753874961627	18.120608879458214
8	26.35537004130019	34.965517662032426	20.174844700712555	18.504267595954833
9	26.52820223839606	15.698760915730276	20.07487976643531	37.69815707943836
10-11	25.273264519512185	25.883264565302888	28.014092683442232	20.829378231742695
12-13	25.697154218437223	23.400011351612818	27.864261819019713	23.038572610930245
14-15	23.13224644833066	25.09117760032586	25.276726664422206	26.499849286921272
16-17	23.03728629544433	27.98700969748989	26.20153775281253	22.77416625425325
18-19	23.099756634934103	27.370606840381388	27.553842233564048	21.97579429112046
20-21	23.59979309469645	26.3660756627552	27.583228471606517	22.450902770941834
22-23	23.627318694381323	26.123250750053707	27.161584644755454	23.087845910809516
24-25	23.416948146300733	26.375415442252848	27.06887531039568	23.138761101050736
26-27	23.043988248926357	26.636845637458812	27.159947672853335	23.1592184407615
28-29	23.14768674440352	26.955170363597887	26.924197166426882	22.972945725571712
30-31	23.763151353011978	26.385635652894596	26.768664122971725	23.0825488711217
32-33	23.45164129462644	26.488745509946998	26.595008826751986	23.46460436867457
34-35	23.708538575328262	26.68135767261932	26.6962520142945	22.913851737757916
36-37	23.711365358367097	26.511347602324282	26.84154561608725	22.93574142322138
38-39	23.626430598500573	26.547011732813758	26.703917997373882	23.122639671311788
40-41	23.775454446103968	26.376469531757646	26.632790943355307	23.215285078783083
42-43	23.629155554772353	26.44620397244256	26.388017436983084	23.536623035802002
44-45	23.482861272866305	26.43159631777078	26.582720958576534	23.502821450786385
46-47	23.64309265567223	26.31943516041365	26.37040343046279	23.66706875345133
48-49	23.350626328131312	26.51818085850679	26.518604471078188	23.6125883422837
50-51	23.529838345799053	26.446584576982502	26.53869021245463	23.484886864763816
52-53	23.77380484432818	26.328818602003334	26.38517085270477	23.512205700963715
54-55	23.133538240082462	26.258747378306758	26.72897905644288	23.8787353251679
56-57	23.30415069020029	26.306449945864983	26.497506739343702	23.891892624591023
58-59	23.39897734221826	26.31589203143986	26.57890184169561	23.70622878464627
60-61	23.546438532103874	26.348067871049075	26.72578442436011	23.379709172486944
62-63	23.48164532926768	26.25155033577405	26.732372603874406	23.53443173108387
64-65	23.11258316308354	26.123707957453313	26.675913882925766	24.08779499653738
66-67	23.220543262841446	26.281839526903056	26.490601637249522	24.007015573005976
68-69	23.15904858310916	26.193029186200594	26.617068581309354	24.030853649380887
70-71	23.18427890072716	26.045566698888145	26.50751526621875	24.26263913416594
72-73	23.144672420776484	26.348846155801592	26.32437452442164	24.18210689900029
74-75	23.08621655634728	26.371835018826435	26.37891886890234	24.163029555923945
76-77	23.09879993926839	25.979509534489477	26.52009900475561	24.401591521486527
78-79	23.00617199807672	26.13591280942218	26.53879488363671	24.319120308864388
80-81	23.267501371840595	26.223964059047884	26.302146682603805	24.206387886507716
82-83	23.16540747266945	26.234634588019617	26.459749089857187	24.140208849453746
84-85	23.142683302996097	26.23813203984855	26.28902741197965	24.3301572451757
86-87	22.846224307046082	26.501458091773472	26.248568445191587	24.40374915598886
88-89	22.79209872868824	26.183022211304767	26.41089580278725	24.613983257219747
90-91	22.82053997147632	26.027643594418524	26.48774129421897	24.664075139886187
92-93	22.98831831069286	25.89877338363519	26.49974115652013	24.613167149151817
94-95	22.8273256741906	25.908366805230663	26.48913938169078	24.775168138887956
96-97	22.60594801334131	25.764326288771244	26.618305197784764	25.011420500102684
98-99	22.35194055088027	25.76257287549198	26.503585957076947	25.381900616550805
100-101	22.36843933311443	25.87461721783571	26.50597694208684	25.250966506963017
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	177.0
1	128.0
2	89.5
3	109.0
4	119.5
5	128.0
6	187.5
7	406.5
8	616.5
9	688.0
10	597.5
11	465.5
12	448.5
13	481.5
14	544.5
15	595.5
16	653.5
17	703.5
18	810.5
19	949.0
20	1049.5
21	1228.0
22	1488.0
23	1816.0
24	2211.5
25	2888.0
26	4075.5
27	5954.0
28	8886.0
29	13225.0
30	19019.0
31	26481.5
32	35863.5
33	43175.5
34	48860.5
35	55505.5
36	63830.5
37	72319.5
38	78513.5
39	84633.0
40	89355.5
41	93097.0
42	97487.0
43	101590.5
44	105971.0
45	112012.0
46	116532.0
47	119113.5
48	121915.5
49	123319.5
50	122994.0
51	121336.0
52	119213.5
53	117388.5
54	114125.0
55	107569.5
56	99330.0
57	89973.0
58	80357.0
59	69314.0
60	56155.5
61	45506.0
62	37561.5
63	28832.0
64	20527.0
65	15012.5
66	11385.5
67	8752.0
68	6830.0
69	5230.5
70	3842.0
71	2853.5
72	2046.0
73	1453.0
74	1130.0
75	802.0
76	523.5
77	330.5
78	207.0
79	117.5
80	65.5
81	37.0
82	24.5
83	20.5
84	16.5
85	11.5
86	6.5
87	6.0
88	5.5
89	2.0
90	2.5
91	3.0
92	1.5
93	1.5
94	0.5
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14587627590913452
2	3.727384518003436E-4
3	0.005760503346005311
4	0.004743943932004374
5	0.026023920998423995
6	0.001423183179601312
7	1.694265690001562E-4
8	0.0018636922590017182
9	0.0
10-11	0.09943645334619168
12-13	2.8802516730026557E-4
14-15	0.005404707551104984
16-17	2.2025453970020306E-4
18-19	3.049678242002812E-4
20-21	7.624195605007029E-4
22-23	3.049678242002812E-4
24-25	0.0019992335142018434
26-27	0.0027616530747025464
28-29	0.006522922906506014
30-31	0.014790939473713637
32-33	0.01497730869961381
34-35	0.01172431857481081
36-37	7.793622174007185E-4
38-39	2.0331188280018746E-4
40-41	0.00503196909930464
42-43	0.009369289265708639
44-45	0.008657697675907981
46-47	0.009335403951908607
48-49	0.010894128386710044
50-51	0.0058113313167053576
52-53	0.0017281510038015934
54-55	0.0011012726985010154
56-57	0.005963815228805498
58-59	0.009843683658909076
60-61	0.00816636062580753
62-63	0.01899271838491751
64-65	0.03571512074523293
66-67	0.03803626474053507
68-69	0.027633473403925475
70-71	0.0418653051999386
72-73	0.026295003508824244
74-75	0.02568506786042368
76-77	0.23821375601421965
78-79	0.06794005416906264
80-81	0.05511446289575082
82-83	0.02143246097851976
84-85	0.032563786561830024
86-87	0.08179914751327542
88-89	0.2654067203387447
90-91	0.31731902108039256
92-93	0.04987918191364599
94-95	0.005015026442404624
96-97	0.01006393819860928
98-99	0.0012198712968011249
100-101	0.0013554125520012497
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2951131.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.8950011744648
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.88664192210518	43.93457223206469
2	13.387698516854938	15.501616427133946
3	4.4571322225263135	7.741370257737174
4	2.055445928646566	4.760001778121673
5	1.124347975759	3.2547063688537214
6	0.7153252944046088	2.4848255255807525
7	0.47867290605361196	1.939893792071084
8	0.32864421788131887	1.5221485904176018
9	0.23865975054276783	1.2435485884173918
>10	1.2528641228068231	12.458609208610719
>50	0.05365284022037765	2.1174331983066423
>100	0.01879992722322271	1.924708932438068
>500	0.00146831595534766	0.5974373059393381
>1k	6.46059019990044E-4	0.5191277943072641
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.388531380003124E-5
2	0.0	0.0	0.0	0.0	3.388531380003124E-5
3	0.0	0.0	0.0	0.0	3.388531380003124E-5
4	0.0	0.0	0.0	0.0	3.388531380003124E-5
5	3.388531380003124E-5	3.388531380003124E-5	0.0	0.0	6.777062760006248E-5
6	3.388531380003124E-5	3.388531380003124E-5	0.0	0.0	6.777062760006248E-5
7	3.388531380003124E-5	3.388531380003124E-5	0.0	0.0	6.777062760006248E-5
8	3.388531380003124E-5	3.388531380003124E-5	0.0	0.0	6.777062760006248E-5
9	3.388531380003124E-5	3.388531380003124E-5	0.0	0.0	6.777062760006248E-5
10-11	3.388531380003124E-5	3.388531380003124E-5	0.0	3.388531380003124E-5	6.777062760006248E-5
12-13	3.388531380003124E-5	3.388531380003124E-5	0.0	8.47132845000781E-5	1.694265690001562E-4
14-15	3.388531380003124E-5	3.388531380003124E-5	0.0	1.0165594140009373E-4	1.694265690001562E-4
16-17	3.388531380003124E-5	3.388531380003124E-5	0.0	1.1859859830010934E-4	2.0331188280018746E-4
18-19	3.388531380003124E-5	3.388531380003124E-5	0.0	1.694265690001562E-4	2.0331188280018746E-4
20-21	3.388531380003124E-5	6.777062760006248E-5	0.0	1.694265690001562E-4	2.371971966002187E-4
22-23	3.388531380003124E-5	6.777062760006248E-5	0.0	2.710825104002499E-4	2.371971966002187E-4
24-25	6.777062760006248E-5	6.777062760006248E-5	0.0	3.727384518003437E-4	2.371971966002187E-4
26-27	6.777062760006248E-5	6.777062760006248E-5	0.0	8.132475312007499E-4	2.371971966002187E-4
28-29	6.777062760006248E-5	6.777062760006248E-5	0.0	0.0024058572798022182	2.371971966002187E-4
30-31	6.777062760006248E-5	6.777062760006248E-5	0.0	0.008996550813908296	2.371971966002187E-4
32-33	6.777062760006248E-5	6.777062760006248E-5	0.0	0.026583028676124512	2.710825104002499E-4
34-35	6.777062760006248E-5	6.777062760006248E-5	0.0	0.051929243398547875	3.388531380003124E-4
36-37	6.777062760006248E-5	6.777062760006248E-5	0.0	0.09308295700868582	3.388531380003124E-4
38-39	6.777062760006248E-5	1.0165594140009372E-4	0.0	0.1565162644423443	3.388531380003124E-4
40-41	6.777062760006248E-5	1.3554125520012496E-4	0.0	0.2260319857031084	3.388531380003124E-4
42-43	6.777062760006248E-5	1.3554125520012496E-4	0.0	0.30195203127207837	3.388531380003124E-4
44-45	6.777062760006248E-5	1.3554125520012496E-4	0.0	0.4080300061230762	3.557957949003281E-4
46-47	6.777062760006248E-5	1.3554125520012496E-4	0.0	0.5324399357398909	4.235664225003905E-4
48-49	6.777062760006248E-5	1.3554125520012496E-4	0.0	0.6591540666951077	5.421650208004998E-4
50-51	6.777062760006248E-5	1.3554125520012496E-4	0.0	0.7867153304953254	5.760503346005311E-4
52-53	6.777062760006248E-5	1.3554125520012496E-4	0.0	0.9220532738126501	5.929929915005467E-4
54-55	6.777062760006248E-5	1.3554125520012496E-4	0.0	1.0862106765169015	6.099356484005623E-4
56-57	6.777062760006248E-5	1.3554125520012496E-4	0.0	1.2794247358046795	6.099356484005623E-4
58-59	6.777062760006248E-5	1.3554125520012496E-4	0.0	1.479534456450764	6.099356484005623E-4
60-61	6.777062760006248E-5	1.3554125520012496E-4	0.0	1.6950111669051628	6.099356484005623E-4
62-63	6.777062760006248E-5	1.3554125520012496E-4	0.0	1.922008884051572	6.438209622005936E-4
64-65	6.777062760006248E-5	1.3554125520012496E-4	0.0	2.18051994303201	6.438209622005936E-4
66-67	6.777062760006248E-5	1.3554125520012496E-4	0.0	2.4853522259770915	6.438209622005936E-4
68-69	6.777062760006248E-5	1.3554125520012496E-4	0.0	2.8199527570955	6.438209622005936E-4
70-71	6.777062760006248E-5	1.3554125520012496E-4	0.0	3.1609067845514147	6.438209622005936E-4
72-73	6.777062760006248E-5	1.3554125520012496E-4	0.0	3.522734165308148	6.946489329006405E-4
74-75	6.777062760006248E-5	1.694265690001562E-4	0.0	3.919293992709914	7.963048743007342E-4
76-77	6.777062760006248E-5	1.694265690001562E-4	0.0	4.372950573864732	8.132475312007499E-4
78-79	6.777062760006248E-5	1.694265690001562E-4	0.0	4.878858308899199	8.132475312007499E-4
80-81	6.777062760006248E-5	1.694265690001562E-4	0.0	5.405283601439583	8.132475312007499E-4
82-83	6.777062760006248E-5	1.694265690001562E-4	0.0	5.949210658557686	9.149034726008436E-4
84-85	6.777062760006248E-5	1.694265690001562E-4	0.0	6.526819717593018	9.149034726008436E-4
86-87	6.777062760006248E-5	1.694265690001562E-4	0.0	7.164592151280306	9.149034726008436E-4
88-89	6.777062760006248E-5	1.694265690001562E-4	0.0	7.857072424097744	9.149034726008436E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3180	0.0	56.93984	1
GTATCAA	5920	0.0	42.627632	1
TCAACGC	7485	0.0	33.162434	4
CAACGCA	7650	0.0	32.566612	5
ATCAACG	7625	0.0	32.553547	3
TATCAAC	7750	0.0	32.089733	2
AACGCAG	8110	0.0	30.832489	6
ACGCAGA	9525	0.0	25.802946	7
GTACATG	8950	0.0	25.700449	1
CGCAGAG	9665	0.0	24.93732	8
TACATGG	9085	0.0	23.926382	2
ACATGGG	9400	0.0	22.417728	3
GCAGAGT	10635	0.0	20.964237	9
CATGGGG	7985	0.0	20.565426	4
GAGTACT	4680	0.0	19.413874	12-13
TAAGGTG	1455	0.0	19.279322	5
AGAGTAC	9055	0.0	18.978573	10-11
CAGAGTA	9810	0.0	18.971708	10-11
ATGGGGG	5630	0.0	18.832119	5
GTACTTT	4895	0.0	18.367323	14-15
>>END_MODULE