FastQCFastQC Report
Mon 15 Aug 2016
SRR2921365_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921365_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3004624
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28850.054.771791
GTATCAA53250.041.793791
TCAACGC62900.035.0081564
CAACGCA64900.033.9281965
ATCAACG65850.033.221973
TATCAAC66650.032.6818922
AACGCAG68250.032.5985686
ACGCAGA80550.026.7998317
GTACATG92700.026.7207891
CGCAGAG83850.025.5238678
TACATGG95600.024.9692082
ACATGGG100850.023.1980363
CATGGGG85200.021.8904614
GCAGAGT92550.021.1764819
TATACCG1353.8219718E-421.0913755
TAAGGCG8700.018.5458665
ATGGGGG54750.018.37555
CAGAGTA84750.018.28230510-11
GTATTAA20000.018.269191
AGAGTAC78250.017.79963910-11
Produced by FastQC (version 0.11.5)