##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921365_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3004624
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.54101844357231	31.0	31.0	34.0	27.0	34.0
2	30.596813112056616	33.0	31.0	34.0	27.0	34.0
3	30.641922583324902	33.0	31.0	34.0	26.0	34.0
4	34.10821520429844	37.0	35.0	37.0	30.0	37.0
5	34.103714141935896	37.0	35.0	37.0	30.0	37.0
6	34.15204764389821	37.0	35.0	37.0	30.0	37.0
7	34.075677688789014	37.0	35.0	37.0	30.0	37.0
8	34.044664490465365	37.0	35.0	37.0	30.0	37.0
9	35.617284891553815	39.0	37.0	39.0	30.0	39.0
10-11	35.5177812598182	39.0	37.0	39.0	30.0	39.0
12-13	35.40855927397239	39.0	37.0	39.0	30.0	39.0
14-15	36.56012865503304	40.0	37.0	41.0	29.5	41.0
16-17	36.504033449776074	40.0	37.0	41.0	29.0	41.0
18-19	36.46153179233075	40.0	37.0	41.0	29.0	41.0
20-21	36.40604232010395	40.0	37.0	41.0	29.0	41.0
22-23	36.29090511824441	40.0	37.0	41.0	28.0	41.0
24-25	36.1910621761658	40.0	36.5	41.0	27.0	41.0
26-27	36.04653860183504	40.0	36.0	41.0	27.0	41.0
28-29	35.90809498958938	40.0	36.0	41.0	27.0	41.0
30-31	35.768687030390495	40.0	36.0	41.0	27.0	41.0
32-33	35.59253454009553	40.0	35.5	41.0	26.0	41.0
34-35	35.43489518156016	39.5	35.0	41.0	25.0	41.0
36-37	35.26716437730644	39.0	35.0	41.0	25.0	41.0
38-39	35.494072802453815	39.5	35.5	41.0	25.5	41.0
40-41	35.62129637518704	40.0	36.0	41.0	26.0	41.0
42-43	35.45741763362071	40.0	35.0	41.0	25.0	41.0
44-45	35.34130443609583	40.0	35.0	41.0	24.0	41.0
46-47	35.198796088961544	40.0	35.0	41.0	24.0	41.0
48-49	35.056940735346586	40.0	35.0	41.0	23.0	41.0
50-51	33.352099630436285	37.0	32.5	39.5	20.0	40.0
52-53	33.51354761860386	37.5	33.5	39.5	21.0	40.0
54-55	34.37411003839415	39.0	34.0	40.5	22.0	41.0
56-57	34.389550905537604	39.0	34.0	41.0	22.0	41.0
58-59	34.21156341026365	38.5	34.0	41.0	21.0	41.0
60-61	33.96971068592942	38.0	34.0	40.5	20.0	41.0
62-63	33.688207076825584	37.5	34.0	40.0	18.5	41.0
64-65	33.40137118654447	37.0	34.0	40.0	18.0	41.0
66-67	33.06792929830821	36.5	33.0	39.5	15.5	41.0
68-69	32.7058966446384	36.0	33.0	39.0	13.0	41.0
70-71	32.328391505892256	35.5	33.0	39.0	9.5	41.0
72-73	31.972977983268457	35.0	33.0	37.5	7.5	40.0
74-75	31.576997321461853	35.0	32.0	37.0	4.5	39.0
76-77	31.197146797735755	35.0	32.0	37.0	2.0	39.0
78-79	30.85131300954795	35.0	32.0	36.0	2.0	39.0
80-81	30.505012108004195	35.0	31.5	36.0	2.0	37.0
82-83	30.204100246819568	35.0	31.0	35.0	2.0	37.0
84-85	29.969134906730424	35.0	31.0	35.0	2.0	36.5
86-87	29.744274657993813	35.0	31.0	35.0	2.0	36.0
88-89	29.543966233378953	35.0	31.0	35.0	2.0	36.0
90-91	29.294182733014182	35.0	31.0	35.0	2.0	35.5
92-93	29.10715384021428	35.0	31.0	35.0	2.0	35.0
94-95	28.904148905154187	34.0	30.0	35.0	2.0	35.0
96-97	28.65488077709557	34.0	30.0	35.0	2.0	35.0
98-99	28.42744216913664	34.0	30.0	35.0	2.0	35.0
100-101	27.38163910026679	33.5	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	96540.0
3	16524.0
4	11620.0
5	8728.0
6	8117.0
7	8242.0
8	8701.0
9	9161.0
10	10019.0
11	10821.0
12	10767.0
13	11014.0
14	11916.0
15	12856.0
16	12567.0
17	13295.0
18	12626.0
19	11766.0
20	11481.0
21	12341.0
22	13593.0
23	15570.0
24	18596.0
25	21889.0
26	25643.0
27	30808.0
28	36575.0
29	43326.0
30	51563.0
31	62360.0
32	79073.0
33	102670.0
34	145957.0
35	211314.0
36	354556.0
37	647182.0
38	704406.0
39	130433.0
40	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.47897462809922	18.417421279310684	13.702204998458253	24.401399094131836
2	16.166137781452797	19.822355843118736	38.97258510811537	25.038921267313096
3	18.972992196147224	23.959120012040763	29.56060539463467	27.507282397177345
4	13.766949690152721	16.2926154480406	36.386816746705136	33.55361811510155
5	14.658449993706633	37.05021573927747	34.02939967940458	14.261934587611325
6	33.212320778031064	34.85943857985897	17.488588913270416	14.439651728839559
7	28.751239520043192	31.8611990531881	21.10920525253588	18.27835617423283
8	26.80282580024061	33.92278516155277	20.310489162239843	18.96389987596678
9	27.044476542035323	15.535315357720773	19.708937951081097	37.71127014916281
10-11	25.57516094985841	25.186224799335942	27.906281151551944	21.332333099253702
12-13	26.145442457761508	22.84186747714941	27.529353172383107	23.48333689270597
14-15	23.636429624558648	24.47880525769722	24.88973766120644	26.99502745653769
16-17	23.626273202285812	27.28979989979176	25.861299810736266	23.22262708718616
18-19	23.61548271806445	26.777237853109792	27.03300390789759	22.574275520928175
20-21	24.25282161782465	25.904852741553043	26.91436813321715	22.92795750740516
22-23	24.196793550673885	25.656955506382552	26.521225407923506	23.625025535020065
24-25	24.000244392010778	25.880747686391775	26.43307696034273	23.68593096125471
26-27	23.6930076006512	26.15479626725794	26.47551844564519	23.67667768644567
28-29	23.747206012791963	26.392632745288736	26.37041233916629	23.489748902753014
30-31	24.282092015181913	25.966781865251775	26.1795816402359	23.571544479330413
32-33	23.94349859177526	26.025321250488254	26.067061221453347	23.964118936283135
34-35	24.26151587874834	26.24120852236605	26.052131423645204	23.445144175240404
36-37	24.262876815641157	26.052707427911866	26.181926561596175	23.502489194850803
38-39	24.093482795124324	26.117464426215403	26.133623926978363	23.65542885168191
40-41	24.251918028754705	25.926296972192205	26.18938418388047	23.632400815172623
42-43	24.14004544222792	25.907489629583306	25.91239238738457	24.040072540804204
44-45	23.992122841525536	25.933186864778225	26.13333351116737	23.94135678252887
46-47	24.119357226186803	25.890504899133717	25.946893247120183	24.043244627559297
48-49	23.910432745780675	26.02695280632937	26.017199257663247	24.04541519022671
50-51	24.017347993731835	26.021890563189583	26.14860100831504	23.812160434763545
52-53	24.227306210061467	25.81279280198558	26.135002119161925	23.82489886879103
54-55	23.711113660221123	25.70563292549055	26.403262831174963	24.179990583113362
56-57	23.82983917401452	25.851598680229436	26.162181973529552	24.15638017222649
58-59	23.95710900643835	25.789620503613016	26.267948990077773	23.98532149987086
60-61	23.9849579175867	25.811091534793317	26.4452708518633	23.758679695756673
62-63	23.966220044601066	25.725234218962754	26.452411022139454	23.85613471429672
64-65	23.741636631491538	25.58168191978336	26.372075999623153	24.304605449101945
66-67	23.76533298653315	25.702612172353025	26.266279184152562	24.265775656961264
68-69	23.782121543254604	25.712340228011566	26.34796022385668	24.157578004877152
70-71	23.72505798278745	25.572255480004795	26.314558518147823	24.38812801905993
72-73	23.64359572523197	25.855001119490012	26.188997588803563	24.31240556647445
74-75	23.72415802857227	25.85713397372237	26.229887537087386	24.188820460617976
76-77	23.674913781476825	25.558459467916638	26.29642034128648	24.470206409320053
78-79	23.636386678790824	25.62406586265473	26.379413301298698	24.360134157255743
80-81	23.790050035496634	25.821805056848817	26.111331523230074	24.27681338442447
82-83	23.783928733129958	25.776289947657826	26.292424101180305	24.14735721803191
84-85	23.78055826951159	25.832892680256293	26.200706679133358	24.18584237109876
86-87	23.592741326187006	25.997405519791666	26.164522334445113	24.245330819576218
88-89	23.503691196366578	25.723245664361976	26.318803820218122	24.454259319053325
90-91	23.6328958248623	25.628685629999858	26.38913581597626	24.349282729161587
92-93	23.751402471151252	25.47526405319041	26.4448642661442	24.328469209514136
94-95	23.696111612452313	25.47486834928265	26.444621628248733	24.3843984100163
96-97	23.570714411770492	25.365876360161987	26.51692444009716	24.546484787970357
98-99	23.379733215825585	25.37516889375984	26.465545839780386	24.779552050634187
100-101	23.586741290291695	25.395346137313318	26.527711218380855	24.490201354014125
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	98.0
1	74.0
2	72.0
3	101.5
4	98.5
5	120.0
6	198.5
7	355.0
8	530.5
9	631.5
10	599.5
11	512.5
12	544.0
13	631.0
14	703.5
15	794.5
16	848.5
17	943.0
18	1077.5
19	1166.5
20	1283.0
21	1399.5
22	1553.0
23	1718.0
24	1911.0
25	2285.5
26	2846.0
27	3723.5
28	5269.5
29	7947.5
30	12148.5
31	18219.5
32	26249.0
33	33472.0
34	39790.0
35	47024.0
36	56152.5
37	65478.5
38	73097.5
39	79558.5
40	84981.5
41	90370.0
42	96271.5
43	102943.5
44	108937.0
45	115543.5
46	120794.0
47	124680.5
48	129332.0
49	131663.0
50	131713.0
51	130087.5
52	127939.0
53	126477.5
54	123334.5
55	116999.0
56	108015.0
57	96662.0
58	86183.0
59	74839.5
60	61732.0
61	51529.0
62	43078.0
63	33250.5
64	23944.5
65	17600.5
66	13383.0
67	10281.5
68	7867.5
69	6081.0
70	4548.0
71	3296.0
72	2358.5
73	1743.5
74	1354.0
75	1004.5
76	740.0
77	500.0
78	297.0
79	205.0
80	156.5
81	106.5
82	81.5
83	85.5
84	72.0
85	58.5
86	49.0
87	37.0
88	33.5
89	39.0
90	35.0
91	19.0
92	17.0
93	18.0
94	17.5
95	18.5
96	14.5
97	7.0
98	5.0
99	8.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05118776925166011
2	0.05880935518054838
3	0.04965679565895766
4	0.05634648461837488
5	0.04865833462023867
6	0.017572914281454185
7	0.014544249130673257
8	0.01963640042814009
9	0.019669682462764057
10-11	0.021982783869129715
12-13	0.027208063305092418
14-15	0.04433167011912306
16-17	0.029254908434466344
18-19	0.023530398479144145
20-21	0.02684196092422879
22-23	0.046445079317744914
24-25	0.04168574836651774
26-27	0.031085420338784486
28-29	0.020917758761162794
30-31	0.013029916555282791
32-33	0.010300789716117557
34-35	0.009784918179446081
36-37	0.0012647173157107178
38-39	0.0066397659074812695
40-41	0.0035611777047643896
42-43	0.21005956152916305
44-45	0.18548077895936396
46-47	0.19228695503996507
48-49	0.19022346889327915
50-51	0.18839295698896102
52-53	0.19293595471513242
54-55	0.1915713912955498
56-57	0.2021051552540351
58-59	0.1981113110991592
60-61	0.20312025731006608
62-63	0.20105677116338017
64-65	0.20157264270005162
66-67	0.19233687809190103
68-69	0.1910721607761903
70-71	0.17448106651614312
72-73	0.18273501110288676
74-75	0.18163670396029585
76-77	0.18017229443684135
78-79	0.2064318197551507
80-81	0.21475232841114228
82-83	0.21208976564122498
84-85	0.20746356282849368
86-87	0.22368855470767723
88-89	0.22232399128809463
90-91	0.22175819669948718
92-93	0.22600165611404288
94-95	0.23660198414177613
96-97	0.23613603565704064
98-99	0.22656745070265033
100-101	0.2360528305704807
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3004624.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.33833322690867
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.16196199777747	47.161625086908685
2	12.218473893882173	14.744846986666937
3	4.011983656800497	7.262292203548201
4	1.7797375683011123	4.295455938104026
5	0.9993290567130062	3.014892481364084
6	0.6060079000142254	2.193930396551849
7	0.4251992950150268	1.7959071725324336
8	0.31741216652075943	1.5321696859043685
9	0.22392687743872677	1.2160237094423132
>10	1.1909374640968309	12.513605705821465
>50	0.04982929629277588	2.026478927711612
>100	0.013596823032203519	1.4280262621521216
>500	0.0012168307086909567	0.5030376469331043
>1k	3.87173406434528E-4	0.3117077963588143
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.984610387189879E-5
2	0.0	0.0	0.0	0.0	1.664101731198313E-4
3	0.0	3.328203462396626E-5	0.0	0.0	1.664101731198313E-4
4	0.0	3.328203462396626E-5	0.0	0.0	1.9969220774379757E-4
5	0.0	3.328203462396626E-5	0.0	0.0	1.9969220774379757E-4
6	0.0	3.328203462396626E-5	0.0	0.0	2.3297424236776382E-4
7	0.0	3.328203462396626E-5	0.0	0.0	2.3297424236776382E-4
8	0.0	3.328203462396626E-5	0.0	0.0	2.3297424236776382E-4
9	0.0	3.328203462396626E-5	0.0	0.0	2.3297424236776382E-4
10-11	0.0	3.328203462396626E-5	0.0	3.328203462396626E-5	2.3297424236776382E-4
12-13	0.0	3.328203462396626E-5	0.0	9.984610387189879E-5	5.491535712954434E-4
14-15	0.0	3.328203462396626E-5	0.0	9.984610387189879E-5	5.990766232313927E-4
16-17	0.0	3.328203462396626E-5	0.0	1.3312813849586505E-4	6.323586578553589E-4
18-19	0.0	3.328203462396626E-5	0.0	1.4976915580784819E-4	6.656406924793252E-4
20-21	0.0	3.328203462396626E-5	0.0	2.1633322505578068E-4	6.656406924793252E-4
22-23	0.0	3.328203462396626E-5	0.0	2.828972943037132E-4	6.656406924793252E-4
24-25	0.0	3.328203462396626E-5	0.0	5.15871536671477E-4	6.656406924793252E-4
26-27	0.0	3.328203462396626E-5	0.0	8.320508655991565E-4	7.488457790392408E-4
28-29	0.0	3.328203462396626E-5	0.0	0.0024795115794854863	7.65486796351224E-4
30-31	0.0	3.328203462396626E-5	0.0	0.007987688309751903	7.65486796351224E-4
32-33	0.0	3.328203462396626E-5	0.0	0.022781552700104905	7.65486796351224E-4
34-35	0.0	3.328203462396626E-5	0.0	0.04546325929633791	7.65486796351224E-4
36-37	0.0	3.328203462396626E-5	0.0	0.08152434381140536	7.65486796351224E-4
38-39	0.0	3.328203462396626E-5	0.0	0.13905234065893102	7.65486796351224E-4
40-41	0.0	3.328203462396626E-5	0.0	0.20275415492920246	7.987688309751903E-4
42-43	0.0	3.328203462396626E-5	0.0	0.27346183748781877	7.987688309751903E-4
44-45	0.0	3.328203462396626E-5	0.0	0.3706786606244242	8.154098482871734E-4
46-47	0.0	3.328203462396626E-5	0.0	0.48270598916869467	8.486918829111395E-4
48-49	0.0	3.328203462396626E-5	0.0	0.5963474963922275	8.98614934847089E-4
50-51	3.328203462396626E-5	3.328203462396626E-5	0.0	0.7155471033979626	8.98614934847089E-4
52-53	6.656406924793252E-5	3.328203462396626E-5	0.0	0.8422684502287141	9.485379867830385E-4
54-55	6.656406924793252E-5	3.328203462396626E-5	0.0	0.9990268333075952	9.984610387189878E-4
56-57	6.656406924793252E-5	3.328203462396626E-5	0.0	1.1830099207088807	9.984610387189878E-4
58-59	6.656406924793252E-5	3.328203462396626E-5	0.0	1.3741320045370071	9.984610387189878E-4
60-61	6.656406924793252E-5	3.328203462396626E-5	0.0	1.5730088024325175	9.984610387189878E-4
62-63	6.656406924793252E-5	3.328203462396626E-5	0.0	1.7839004148272797	0.0010983071425908866
64-65	6.656406924793252E-5	4.992305193594939E-5	0.0	2.0231483207216607	0.0011482301945268361
66-67	6.656406924793252E-5	6.656406924793252E-5	0.0	2.3167957122089153	0.001164871211838819
68-69	6.656406924793252E-5	6.656406924793252E-5	0.0	2.6287482227393513	0.001164871211838819
70-71	6.656406924793252E-5	6.656406924793252E-5	0.0	2.9524160094574228	0.0011981532464627855
72-73	9.984610387189879E-5	6.656406924793252E-5	0.0	3.292342070089302	0.0013812044368946
74-75	9.984610387189879E-5	6.656406924793252E-5	0.0	3.6632869869907183	0.001397845454206583
76-77	9.984610387189879E-5	6.656406924793252E-5	0.0	4.0953710014963605	0.001414486471518566
78-79	9.984610387189879E-5	6.656406924793252E-5	0.0	4.570122584389927	0.001431127488830549
80-81	9.984610387189879E-5	6.656406924793252E-5	0.0	5.055724110570907	0.0014477685061425323
82-83	9.984610387189879E-5	6.656406924793252E-5	0.0	5.567069290533524	0.0014976915580784816
84-85	9.984610387189879E-5	6.656406924793252E-5	0.0	6.11271160717614	0.0014976915580784816
86-87	9.984610387189879E-5	6.656406924793252E-5	0.0	6.712786691446251	0.0016141786792623638
88-89	9.984610387189879E-5	6.656406924793252E-5	0.0	7.3683762094691385	0.0016973837658222793
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2885	0.0	54.77179	1
GTATCAA	5325	0.0	41.79379	1
TCAACGC	6290	0.0	35.008156	4
CAACGCA	6490	0.0	33.928196	5
ATCAACG	6585	0.0	33.22197	3
TATCAAC	6665	0.0	32.681892	2
AACGCAG	6825	0.0	32.598568	6
ACGCAGA	8055	0.0	26.799831	7
GTACATG	9270	0.0	26.720789	1
CGCAGAG	8385	0.0	25.523867	8
TACATGG	9560	0.0	24.969208	2
ACATGGG	10085	0.0	23.198036	3
CATGGGG	8520	0.0	21.890461	4
GCAGAGT	9255	0.0	21.176481	9
TATACCG	135	3.8219718E-4	21.091375	5
TAAGGCG	870	0.0	18.545866	5
ATGGGGG	5475	0.0	18.3755	5
CAGAGTA	8475	0.0	18.282305	10-11
GTATTAA	2000	0.0	18.26919	1
AGAGTAC	7825	0.0	17.799639	10-11
>>END_MODULE