FastQCFastQC Report
Mon 15 Aug 2016
SRR2921365_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921365_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3004624
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27350.055.087411
GTATCAA50250.042.1841431
TCAACGC62250.033.850464
CAACGCA64600.032.8277475
ATCAACG65300.032.342073
TATCAAC65950.032.0233082
AACGCAG69850.030.3558226
GTACATG86050.026.1252671
ACGCAGA83850.024.6633787
TACATGG91200.024.0939032
CGCAGAG86850.023.0998388
ACATGGG94900.022.4543823
CATGGGG77850.021.3368554
ATGGGGG50200.019.3225845
GCAGAGT96000.019.1153569
TACACCG4650.017.38345
AGAGTAC83000.017.29797210-11
GTATATG10000.017.1101171
TACACTG39900.016.8029295
CAGAGTA91350.016.7316510-11
Produced by FastQC (version 0.11.5)