##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921365_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3004624
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.29708276310114	33.0	31.0	34.0	28.0	34.0
2	31.43699278179233	34.0	31.0	34.0	28.0	34.0
3	31.50471672994691	34.0	31.0	34.0	28.0	34.0
4	34.996876813870884	37.0	35.0	37.0	32.0	37.0
5	34.725182918062295	37.0	35.0	37.0	32.0	37.0
6	34.675582036221506	37.0	35.0	37.0	32.0	37.0
7	34.53806998812497	37.0	35.0	37.0	31.0	37.0
8	34.49739434950929	37.0	35.0	37.0	31.0	37.0
9	36.10265111375001	39.0	37.0	39.0	32.0	39.0
10-11	35.95447966201428	39.0	37.0	39.0	31.0	39.0
12-13	35.884894083253016	39.0	37.0	39.0	30.5	39.0
14-15	37.11124270457801	40.0	38.0	41.0	31.0	41.0
16-17	37.086625814078566	40.0	38.0	41.0	31.0	41.0
18-19	37.046966941620646	40.0	38.0	41.0	31.0	41.0
20-21	37.00980854842403	40.0	38.0	41.0	31.0	41.0
22-23	36.901114082827	40.0	37.0	41.0	30.0	41.0
24-25	36.82529278205859	40.0	37.0	41.0	30.0	41.0
26-27	36.68561041248422	40.0	37.0	41.0	30.0	41.0
28-29	36.53260823983301	40.0	37.0	41.0	30.0	41.0
30-31	36.392686905250045	40.0	37.0	41.0	29.5	41.0
32-33	36.3402738578937	40.0	36.5	41.0	29.5	41.0
34-35	36.4672989032904	40.0	37.0	41.0	30.0	41.0
36-37	36.441319279883274	40.0	37.0	41.0	30.0	41.0
38-39	36.3542651260191	40.0	37.0	41.0	29.5	41.0
40-41	36.23219793891016	40.0	36.5	41.0	29.0	41.0
42-43	36.1273853234215	40.0	36.0	41.0	28.5	41.0
44-45	35.99147796862436	40.0	36.0	41.0	28.0	41.0
46-47	35.84004387903445	40.0	36.0	41.0	27.5	41.0
48-49	35.68297031508768	40.0	35.0	41.0	27.0	41.0
50-51	35.52992653989318	39.5	35.0	41.0	26.5	41.0
52-53	35.35816494842616	39.0	35.0	41.0	26.0	41.0
54-55	35.150046727976616	39.0	35.0	41.0	26.0	41.0
56-57	34.889603324742126	39.0	35.0	41.0	25.0	41.0
58-59	34.66636723929517	38.5	35.0	40.5	25.0	41.0
60-61	34.39530453727322	38.0	34.0	40.0	24.0	41.0
62-63	34.10135394645054	37.5	34.0	40.0	23.0	41.0
64-65	33.790632371970666	37.0	34.0	40.0	23.0	41.0
66-67	33.46163014074307	36.5	34.0	39.0	22.0	41.0
68-69	33.10208132531724	36.0	33.5	39.0	22.0	41.0
70-71	32.72592943409891	35.0	33.0	39.0	21.0	40.0
72-73	32.33534245882347	35.0	33.0	37.5	20.0	40.0
74-75	31.931612241664844	35.0	33.0	37.0	18.5	39.0
76-77	29.1790383755172	33.0	28.5	34.5	11.0	36.5
78-79	30.83677891143784	34.5	31.5	36.0	13.5	37.5
80-81	31.036135303452276	35.0	32.0	36.0	15.0	37.0
82-83	30.872629320673735	35.0	32.0	35.0	12.0	37.0
84-85	30.640324380022257	35.0	32.0	35.0	8.5	36.5
86-87	30.396031916139922	35.0	32.0	35.0	6.0	36.0
88-89	30.120224693672153	35.0	31.5	35.0	2.0	36.0
90-91	29.938577505870953	34.0	31.0	35.0	2.0	35.0
92-93	29.794366117024957	34.0	31.0	35.0	2.0	35.0
94-95	29.696281132015187	34.0	31.0	35.0	2.0	35.0
96-97	29.50616649537513	34.0	31.0	35.0	2.0	35.0
98-99	29.240554059343197	34.0	31.0	35.0	2.0	35.0
100-101	28.156890179935992	33.0	28.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	63676.0
3	21089.0
4	11197.0
5	7796.0
6	6932.0
7	6789.0
8	7104.0
9	7511.0
10	8346.0
11	8879.0
12	9352.0
13	10304.0
14	11152.0
15	12118.0
16	12949.0
17	13854.0
18	12925.0
19	11600.0
20	10959.0
21	10527.0
22	11374.0
23	13058.0
24	15227.0
25	18415.0
26	21916.0
27	26552.0
28	32011.0
29	38315.0
30	46722.0
31	57728.0
32	74046.0
33	98025.0
34	141756.0
35	212091.0
36	367076.0
37	712417.0
38	756108.0
39	96727.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.922165396532506	20.159768922688027	14.447664961895976	25.47040071888349
2	14.962374484542087	21.25460542299999	37.60258402920845	26.180436063249473
3	17.44355601827829	25.18207226041201	29.504875951883196	27.869495769426507
4	13.247923272067169	17.592326940448384	35.4541841765946	33.70556561088985
5	14.327908332387313	37.38589903685621	34.08445743387812	14.201735196878362
6	33.02881117540517	35.600869068008436	17.245202186781725	14.12511756980468
7	28.387183737044953	32.11534237274597	21.25839540441054	18.239078485798537
8	26.576339399232367	34.60294965379244	20.07424724318668	18.746463703788514
9	26.63451400241761	15.689317531910815	19.699902550202623	37.97626591546896
10-11	25.45842125848629	25.606275440547492	27.820406808888308	21.114896492077907
12-13	25.912579178171185	23.047071572540386	27.677946200113425	23.362403049175004
14-15	23.405230818285997	24.726232275118715	24.946737570074916	26.92179933652037
16-17	23.45008789791195	27.606683968898583	25.80238046582898	23.140847667360486
18-19	23.452972541924645	27.05261602393916	27.04276451873899	22.451646915397202
20-21	24.077749295581516	26.053732169875182	26.957461311067078	22.911057223476224
22-23	24.011042344686036	25.81589890619948	26.58832719194852	23.584731557165966
24-25	23.79732974770226	26.117051106891637	26.457713794084707	23.627905351321395
26-27	23.53892283928326	26.330458468447986	26.60262928155226	23.52798941071649
28-29	23.63814705988079	26.563321701643837	26.36206928688113	23.436461951594243
30-31	24.09980869905035	26.15373912302478	26.206648964588187	23.539803213336675
32-33	23.83658933147503	26.200617241314454	26.01717244360221	23.945620983608304
34-35	24.102735311098304	26.390185879594917	26.141191402209547	23.365887407097233
36-37	24.087569376678253	26.209374122182833	26.244653219783455	23.458403281355462
38-39	23.963196677117242	26.265384640986216	26.161511272640137	23.60990740925641
40-41	24.15160147240636	26.04263414889051	26.190214279093198	23.61555009960993
42-43	23.99563772261561	26.05766753628186	25.956499047728094	23.99019569337444
44-45	23.880788276537515	26.069226743087853	26.10417545585187	23.945809524522762
46-47	24.000131143437635	26.022419536511133	25.938424494665057	24.039024825386175
48-49	23.77551278543464	26.141438974688764	26.105307165315594	23.97774107456101
50-51	23.918536782796707	26.12210296200833	26.087221346483613	23.872138908711353
52-53	24.13559208083777	25.943229107642303	26.04256061277005	23.878618198749876
54-55	23.628569526725688	25.876073354190243	26.28587831990947	24.2094787991746
56-57	23.765693593553962	25.979825595395894	26.02387691193448	24.230603899115664
58-59	23.805270484934873	25.922850778323927	26.182372525377595	24.089506211363602
60-61	23.867312582867644	25.975210810625498	26.304692644773215	23.852783961733643
62-63	23.81825390115022	25.94781689376635	26.26438277641927	23.96954642866416
64-65	23.57733876361719	25.74864772814666	26.246342365569348	24.427671142666803
66-67	23.650992593978597	25.98318434116474	26.029545845368318	24.336277219488345
68-69	23.636715910553043	25.886122798394133	26.10913596629135	24.36802532476147
70-71	23.57766319459994	25.70163976618195	26.125635998510376	24.595061040707733
72-73	23.51418435938682	25.997978608942056	25.956032747067788	24.53180428460334
74-75	23.54438727477792	26.045166255870654	25.913022433323878	24.497424036027542
76-77	23.42668202110078	25.709397540844154	26.120024125854762	24.743896312200302
78-79	23.36754045898617	25.79388900998605	26.148110687280308	24.690459843747476
80-81	23.521899864072797	25.97438361574358	25.90588722637241	24.597829293811216
82-83	23.491235933811495	25.93293435060226	25.984681047110797	24.591148668475444
84-85	23.462128182049867	25.95415094926948	25.920425927384	24.663294941296655
86-87	23.278772396406083	26.120934056060484	25.870953999506035	24.729339548027397
88-89	23.116553772231306	25.906203076512398	25.97734325469652	24.99989989655978
90-91	23.146119819831398	25.820204316387763	26.004242305167203	25.02943355861364
92-93	23.27290666212916	25.70644528775357	26.09189486987934	24.92875318023793
94-95	23.10615900743875	25.704870693478497	26.075035875717827	25.113934423364924
96-97	22.93827652789201	25.61964957782446	26.12071154894004	25.321362345343484
98-99	22.675849752145226	25.649374854805096	26.03220680946634	25.64256858358334
100-101	22.718197209799552	25.73736423674868	26.004971731555788	25.53946682189599
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	88.0
1	71.5
2	60.0
3	67.0
4	69.0
5	85.0
6	147.0
7	316.5
8	449.0
9	465.5
10	442.0
11	376.5
12	351.5
13	394.5
14	446.5
15	497.0
16	548.5
17	605.0
18	667.5
19	740.0
20	842.0
21	977.5
22	1109.0
23	1352.5
24	1665.0
25	2071.0
26	2757.0
27	3871.5
28	5710.0
29	8636.0
30	13099.0
31	19397.0
32	27470.5
33	34420.0
34	40451.0
35	47872.0
36	57217.5
37	65822.5
38	72890.0
39	79993.0
40	85554.0
41	90739.5
42	96906.0
43	103071.5
44	109165.5
45	115818.5
46	121087.0
47	125729.5
48	130531.0
49	132999.5
50	132580.0
51	130546.0
52	128805.5
53	127075.5
54	123946.0
55	117540.0
56	107821.0
57	96268.5
58	85441.5
59	73993.0
60	60886.0
61	50494.0
62	42026.0
63	32210.0
64	22888.0
65	16662.5
66	12658.0
67	9789.0
68	7454.0
69	5616.0
70	4090.0
71	2959.5
72	2113.5
73	1491.5
74	1089.5
75	784.5
76	557.5
77	317.5
78	160.0
79	91.0
80	58.5
81	40.5
82	27.5
83	21.5
84	13.0
85	8.0
86	6.5
87	6.0
88	4.0
89	3.5
90	2.5
91	0.0
92	0.5
93	2.0
94	2.0
95	0.5
96	0.0
97	1.0
98	1.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14697346489943502
2	4.659484847355277E-4
3	0.006157176405433758
4	0.0045929207781073445
5	0.027124858218532503
6	8.653329002231227E-4
7	1.3312813849586505E-4
8	0.0022631783544297054
9	0.0
10-11	0.10044518049513018
12-13	2.662562769917301E-4
14-15	0.005358407574458568
16-17	6.656406924793252E-5
18-19	2.3297424236776385E-4
20-21	5.990766232313927E-4
22-23	2.828972943037132E-4
24-25	0.001297999350334684
26-27	0.002562716666045402
28-29	0.006257022509305658
30-31	0.015209889823152582
32-33	0.014993556598096801
34-35	0.011332532789460512
36-37	3.993844154875951E-4
38-39	1.3312813849586505E-4
40-41	0.005142074349402787
42-43	0.007654867963512241
44-45	0.0073719706692085265
46-47	0.009318969694710553
48-49	0.011515583979892326
50-51	0.005857638093818062
52-53	0.001281358333022701
54-55	7.987688309751902E-4
56-57	0.005774433007258146
58-59	0.008370431707927515
60-61	0.006972586253720932
62-63	0.01697383765822279
64-65	0.03244998375836711
66-67	0.03509590551097242
68-69	0.02547739750464617
70-71	0.03918959576972027
72-73	0.02502809003722263
74-75	0.02329742423677638
76-77	0.23330706271400348
78-79	0.06604819771126104
80-81	0.05145402552865183
82-83	0.018887554649100854
84-85	0.030602830836736976
86-87	0.07936101156084756
88-89	0.2570704354355154
90-91	0.3101386396434296
92-93	0.050172667195629135
94-95	0.005142074349402787
96-97	0.01015102056030971
98-99	0.001397845454206583
100-101	0.0012314352810867517
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3004624.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.85778130200432
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.8752605142602	46.614403126992
2	12.273757189057207	14.693597471529793
3	4.08055638128871	7.327591543850332
4	1.8164246477011103	4.349085972546532
5	1.036815296462622	3.103073163311621
6	0.6424797798691967	2.3074448492622155
7	0.4341455477221974	1.819089247415592
8	0.31432435649860047	1.5051806871349174
9	0.23008152088916942	1.23949524231148
>10	1.2312108996225442	12.821780094005502
>50	0.048990241625324046	1.9570282719338028
>100	0.014391843241675521	1.4858057522073147
>500	0.0012271142413041818	0.500010082769173
>1k	3.3466752012678217E-4	0.2764144947297551
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.328203462396626E-5
2	0.0	0.0	0.0	0.0	3.328203462396626E-5
3	0.0	0.0	0.0	0.0	3.328203462396626E-5
4	0.0	0.0	0.0	0.0	3.328203462396626E-5
5	0.0	3.328203462396626E-5	0.0	0.0	3.328203462396626E-5
6	0.0	3.328203462396626E-5	0.0	0.0	3.328203462396626E-5
7	0.0	3.328203462396626E-5	0.0	0.0	3.328203462396626E-5
8	0.0	3.328203462396626E-5	0.0	0.0	3.328203462396626E-5
9	0.0	3.328203462396626E-5	0.0	0.0	3.328203462396626E-5
10-11	0.0	3.328203462396626E-5	0.0	0.0	3.328203462396626E-5
12-13	0.0	3.328203462396626E-5	0.0	0.0	1.6641017311983132E-4
14-15	0.0	3.328203462396626E-5	0.0	0.0	2.1633322505578068E-4
16-17	0.0	3.328203462396626E-5	0.0	0.0	2.3297424236776382E-4
18-19	0.0	3.328203462396626E-5	0.0	1.664101731198313E-5	2.3297424236776382E-4
20-21	0.0	3.328203462396626E-5	0.0	4.992305193594939E-5	2.4961525967974696E-4
22-23	0.0	3.328203462396626E-5	0.0	1.1648712118388191E-4	3.1617932892767946E-4
24-25	0.0	3.328203462396626E-5	0.0	3.1617932892767946E-4	3.328203462396626E-4
26-27	0.0	3.328203462396626E-5	0.0	5.990766232313927E-4	3.9938441548759514E-4
28-29	0.0	3.328203462396626E-5	0.0	0.002163332250557807	3.9938441548759514E-4
30-31	0.0	3.328203462396626E-5	0.0	0.007804637119320088	3.9938441548759514E-4
32-33	0.0	3.328203462396626E-5	0.0	0.022798193717416888	4.160254327995783E-4
34-35	0.0	4.992305193594939E-5	0.0	0.04533013115784205	5.325125539834602E-4
36-37	0.0	6.656406924793252E-5	0.0	0.0812747285517256	5.325125539834602E-4
38-39	0.0	6.656406924793252E-5	0.0	0.1387028792953794	5.657945886074265E-4
40-41	0.0	6.656406924793252E-5	0.0	0.2025045396695227	5.657945886074265E-4
42-43	0.0	6.656406924793252E-5	0.0	0.27276291476071546	5.657945886074265E-4
44-45	0.0	6.656406924793252E-5	0.0	0.3692974561875296	5.824356059194096E-4
46-47	0.0	6.656406924793252E-5	0.0	0.4806425030220087	6.656406924793252E-4
48-49	0.0	6.656406924793252E-5	0.0	0.593834702778118	6.989227271032915E-4
50-51	0.0	6.656406924793252E-5	0.0	0.7128013355414854	7.322047617272577E-4
52-53	0.0	8.320508655991566E-5	0.0	0.8392397850779332	7.65486796351224E-4
54-55	0.0	9.984610387189879E-5	0.0	0.9944006304948639	8.653329002231227E-4
56-57	0.0	9.984610387189879E-5	0.0	1.1774018978747423	8.653329002231227E-4
58-59	0.0	9.984610387189879E-5	0.0	1.3677085718545814	8.653329002231227E-4
60-61	0.0	9.984610387189879E-5	0.0	1.565536985659437	8.653329002231227E-4
62-63	0.0	9.984610387189879E-5	0.0	1.7748310603922488	8.653329002231227E-4
64-65	0.0	9.984610387189879E-5	0.0	2.013596376784583	8.653329002231227E-4
66-67	0.0	9.984610387189879E-5	0.0	2.305113718055903	8.819739175351059E-4
68-69	0.0	9.984610387189879E-5	0.0	2.6147697681972852	8.98614934847089E-4
70-71	0.0	9.984610387189879E-5	0.0	2.9379716064306214	8.98614934847089E-4
72-73	0.0	9.984610387189879E-5	0.0	3.277747897906693	0.0010483840906549372
74-75	0.0	9.984610387189879E-5	0.0	3.6481603022541256	0.0010983071425908866
76-77	0.0	9.984610387189879E-5	0.0	4.07992813743084	0.0010983071425908866
78-79	0.0	9.984610387189879E-5	0.0	4.557242436990452	0.0010983071425908866
80-81	0.0	9.984610387189879E-5	0.0	5.043892347262087	0.001148230194526836
82-83	0.0	9.984610387189879E-5	0.0	5.555570347570944	0.0012314352810867517
84-85	0.0	9.984610387189879E-5	0.0	6.104041637156596	0.0012314352810867517
86-87	0.0	9.984610387189879E-5	0.0	6.706629515040817	0.0012979993503346842
88-89	0.0	9.984610387189879E-5	0.0	7.36478174972975	0.0013312813849586504
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2735	0.0	55.08741	1
GTATCAA	5025	0.0	42.184143	1
TCAACGC	6225	0.0	33.85046	4
CAACGCA	6460	0.0	32.827747	5
ATCAACG	6530	0.0	32.34207	3
TATCAAC	6595	0.0	32.023308	2
AACGCAG	6985	0.0	30.355822	6
GTACATG	8605	0.0	26.125267	1
ACGCAGA	8385	0.0	24.663378	7
TACATGG	9120	0.0	24.093903	2
CGCAGAG	8685	0.0	23.099838	8
ACATGGG	9490	0.0	22.454382	3
CATGGGG	7785	0.0	21.336855	4
ATGGGGG	5020	0.0	19.322584	5
GCAGAGT	9600	0.0	19.115356	9
TACACCG	465	0.0	17.3834	5
AGAGTAC	8300	0.0	17.297972	10-11
GTATATG	1000	0.0	17.110117	1
TACACTG	3990	0.0	16.802929	5
CAGAGTA	9135	0.0	16.73165	10-11
>>END_MODULE