FastQCFastQC Report
Mon 15 Aug 2016
SRR2921364_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921364_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2366578
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22800.053.9163861
GTATCAA44850.043.4945871
TCAACGC56250.034.510934
CAACGCA56900.033.947715
TATCAAC57650.033.4265672
ATCAACG58750.033.041683
AACGCAG61150.031.7321196
GTACATG82850.026.8107641
ACGCAGA70450.026.6733917
CGCAGAG72450.025.8071618
TACATGG86750.025.5512662
ACATGGG88450.024.3078783
CATGGGG71200.022.5316264
GCAGAGT79950.022.318299
GTATAGG7500.021.5165921
ATGGGGG44250.021.343625
TAAGGTA6050.019.612844
GAGTACA64350.019.3982721
AGAGTAC68300.019.31593110-11
CAGAGTA74050.018.52099810-11
Produced by FastQC (version 0.11.5)