##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921364_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2366578
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.45929988362944	31.0	31.0	34.0	26.0	34.0
2	30.511254647005085	33.0	31.0	34.0	26.0	34.0
3	30.555043188942008	33.0	31.0	34.0	26.0	34.0
4	34.01798673020708	37.0	35.0	37.0	30.0	37.0
5	34.01822927450522	37.0	35.0	37.0	30.0	37.0
6	34.06843298636259	37.0	35.0	37.0	30.0	37.0
7	33.989694402635365	37.0	35.0	37.0	30.0	37.0
8	33.95874507411123	37.0	35.0	37.0	30.0	37.0
9	35.51848407278357	39.0	37.0	39.0	30.0	39.0
10-11	35.41625587662862	39.0	37.0	39.0	30.0	39.0
12-13	35.30639007038855	39.0	35.0	39.0	29.0	39.0
14-15	36.440642353643106	40.0	37.0	41.0	29.0	41.0
16-17	36.393643902715226	40.0	37.0	41.0	29.0	41.0
18-19	36.35158063668301	40.0	37.0	41.0	28.0	41.0
20-21	36.29274716489378	40.0	37.0	41.0	28.0	41.0
22-23	36.17890937885842	40.0	36.5	41.0	27.0	41.0
24-25	36.0824671741223	40.0	36.0	41.0	27.0	41.0
26-27	35.93489544819566	40.0	36.0	41.0	27.0	41.0
28-29	35.79401059250952	40.0	36.0	41.0	27.0	41.0
30-31	35.65139813688795	40.0	36.0	41.0	26.5	41.0
32-33	35.4754476717015	39.5	35.5	41.0	25.5	41.0
34-35	35.310645370657554	39.0	35.0	41.0	25.0	41.0
36-37	35.14385264293	39.0	35.0	41.0	24.5	41.0
38-39	35.37552829444033	39.5	35.0	41.0	25.0	41.0
40-41	35.50494025550816	40.0	35.0	41.0	25.0	41.0
42-43	35.340600647855254	40.0	35.0	41.0	24.0	41.0
44-45	35.216929253969234	40.0	35.0	41.0	24.0	41.0
46-47	35.06206788874062	40.0	35.0	41.0	23.5	41.0
48-49	34.91839123831963	40.0	35.0	41.0	23.0	41.0
50-51	33.20262843650199	37.0	32.5	39.5	18.5	40.0
52-53	33.363422418361026	37.5	33.5	39.5	19.5	40.0
54-55	34.22646792119254	39.0	34.0	40.5	20.0	41.0
56-57	34.245111084443444	39.0	34.0	41.0	20.0	41.0
58-59	34.07114428512392	38.5	34.0	41.0	19.5	41.0
60-61	33.82815609711575	38.0	34.0	40.5	18.0	41.0
62-63	33.5405900418241	37.5	34.0	40.0	16.0	41.0
64-65	33.252471289769446	37.0	33.5	40.0	13.5	41.0
66-67	32.91180430140059	36.0	33.0	39.5	12.0	41.0
68-69	32.5470238039904	36.0	33.0	39.0	8.0	41.0
70-71	32.17011334509152	35.0	33.0	39.0	7.0	40.5
72-73	31.81291954036588	35.0	32.0	37.5	2.0	40.0
74-75	31.415827198596453	35.0	32.0	37.0	2.0	39.0
76-77	31.042296725482956	35.0	32.0	36.5	2.0	39.0
78-79	30.699130558975874	35.0	32.0	36.0	2.0	38.5
80-81	30.356351237947788	35.0	31.0	36.0	2.0	37.0
82-83	30.059390816613693	35.0	31.0	35.0	2.0	37.0
84-85	29.826821258373904	35.0	31.0	35.0	2.0	36.5
86-87	29.60570177699615	35.0	31.0	35.0	2.0	36.0
88-89	29.409344420509278	35.0	31.0	35.0	2.0	36.0
90-91	29.16839313979932	35.0	31.0	35.0	2.0	35.5
92-93	28.98171389237963	34.5	30.0	35.0	2.0	35.0
94-95	28.77864219983453	34.0	30.0	35.0	2.0	35.0
96-97	28.53592338811567	34.0	30.0	35.0	2.0	35.0
98-99	28.315449564730173	34.0	30.0	35.0	2.0	35.0
100-101	27.27254626722635	33.5	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	79946.0
3	13630.0
4	9353.0
5	7108.0
6	6359.0
7	6604.0
8	6987.0
9	7292.0
10	8112.0
11	8667.0
12	8753.0
13	8723.0
14	9557.0
15	10393.0
16	10634.0
17	11497.0
18	10493.0
19	9329.0
20	9385.0
21	10016.0
22	10780.0
23	12433.0
24	14296.0
25	17308.0
26	20534.0
27	24433.0
28	28686.0
29	34006.0
30	41070.0
31	49889.0
32	62329.0
33	81895.0
34	115421.0
35	167790.0
36	277722.0
37	509869.0
38	546565.0
39	98712.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.55070539952347	18.606846509866124	13.71656686964271	24.125881220967692
2	15.956859412587884	19.58829572695103	39.64008641646479	24.814758443996297
3	19.2857553523749	24.10022946600538	29.222208318607713	27.391806863012008
4	13.562285758737492	16.01898936467055	36.78020431873181	33.638520557860154
5	14.496615923127978	37.207343932969486	33.97579359878925	14.320246545113275
6	33.057795704605184	35.129969968243934	17.469260346502058	14.342973980648832
7	29.037757482040018	31.90311351418056	20.900984814259626	18.1581441895198
8	26.93535612581368	34.371806456357334	19.9022696401085	18.790567777720486
9	27.03052577259024	16.061458121574795	19.50717193152104	37.400844174313924
10-11	25.753978492987624	25.38706134720277	27.52121612863211	21.337744031177497
12-13	26.314357983487007	22.79837892132798	27.498065792023564	23.38919730316145
14-15	23.680261889981434	24.36054090054695	24.842305681899123	27.11689152757249
16-17	23.70049061616022	27.440691237276262	25.60046324816851	23.25835489839501
18-19	23.652123783355883	26.82373521631318	26.963039884836963	22.56110111549397
20-21	24.209653184443493	25.918413494762156	27.0199348677384	22.851998453055955
22-23	24.229856029267193	25.542846791745816	26.670846164398416	23.556451014588575
24-25	23.933292780204436	25.880413641914746	26.566068698240336	23.62022487964048
26-27	23.605180418792997	26.162864789091373	26.620582149361095	23.61137264275454
28-29	23.766318457856336	26.307579236628577	26.496983021492248	23.42911928402284
30-31	24.18685181792614	25.90970262836438	26.356035830901774	23.547409722807704
32-33	23.855379987685694	25.97767535105709	26.305246934840238	23.86169772641698
34-35	24.16850335569477	26.09256321073315	26.300731865376665	23.438201568195414
36-37	24.201525178287486	25.915924620849907	26.392505861926026	23.490044338936585
38-39	24.0326155189306	26.09374626941673	26.29707032079477	23.5765678908579
40-41	24.13986974366017	25.904975200587366	26.3662918143451	23.58886324140736
42-43	23.99428730632012	25.879090914479807	26.160830195134306	23.96579158406577
44-45	23.8612768735102	25.929796890087147	26.326039223355714	23.882887013046936
46-47	24.12446239011528	25.862551787185755	26.08886705417406	23.924118768524906
48-49	23.895881676488226	26.05663152551894	26.1110926493146	23.936394148678232
50-51	23.882435315039814	26.120379789328595	26.21907777180891	23.778107123822682
52-53	24.199216319121355	25.895441764535054	26.165890408291304	23.739451508052287
54-55	23.655562566423622	25.792739031384016	26.45068348202559	24.10101492016677
56-57	23.75049720159384	25.9457202982511	26.243861319970474	24.05992118018459
58-59	23.89273079164459	25.89388360943312	26.32222125083905	23.891164348083237
60-61	23.94715517029688	25.917426143152177	26.438884405745462	23.696534280805476
62-63	23.91596703050358	25.801571252177443	26.493399771337995	23.789061945980983
64-65	23.650685286736586	25.68117469863578	26.467934042276198	24.20020597235144
66-67	23.7593477280761	25.75155416231868	26.334907427594135	24.154190682011087
68-69	23.755871843788054	25.79682769624335	26.406549075628437	24.040751384340155
70-71	23.737891600419797	25.612253125787674	26.393861416270326	24.255993857522196
72-73	23.709726883468683	25.9064394445264	26.148606733825304	24.235226938179615
74-75	23.679991500235882	26.022590832234	26.25533960219157	24.04207806533855
76-77	23.727949771330962	25.607228519406867	26.386495859957066	24.278325849305105
78-79	23.660952349500363	25.67862941066513	26.376570812716764	24.283847427117742
80-81	23.837894542956317	25.92171437826371	26.149836609436743	24.090554469343232
82-83	23.85980177645389	25.86416087376719	26.273839805820103	24.002197543958818
84-85	23.785401059941258	25.924484795469954	26.18580769395244	24.10430645063635
86-87	23.607882428842363	26.12508652068588	26.18272110177331	24.084309948698447
88-89	23.564626530187052	25.791073040759255	26.2950798568353	24.349220572218393
90-91	23.70014147745486	25.791115588478036	26.315420619121603	24.193322314945497
92-93	23.856520738434668	25.622104340199808	26.38919149885841	24.132183422507108
94-95	23.736691398849974	25.631649006569223	26.341956351134037	24.289703243446763
96-97	23.65787811364633	25.444501078601885	26.506712700566272	24.39090810718552
98-99	23.40668987617034	25.474286532206257	26.437787251486306	24.681236340137097
100-101	23.662558019717864	25.48170739067284	26.50715739752329	24.348577192086005
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	61.0
1	52.0
2	64.0
3	81.0
4	85.0
5	110.0
6	155.0
7	339.0
8	569.0
9	632.0
10	558.5
11	471.5
12	472.5
13	513.5
14	598.5
15	679.0
16	732.0
17	788.5
18	890.0
19	1003.5
20	1097.0
21	1205.5
22	1327.0
23	1521.0
24	1733.0
25	2064.0
26	2578.0
27	3379.5
28	4456.0
29	6181.5
30	9127.0
31	13261.5
32	18946.5
33	24538.5
34	30083.0
35	36659.0
36	45089.5
37	53609.5
38	60089.0
39	65739.0
40	70100.0
41	73576.5
42	76952.5
43	81465.5
44	86223.0
45	91101.0
46	94713.0
47	97840.5
48	102146.5
49	103908.0
50	102439.0
51	100828.0
52	99725.0
53	98542.5
54	96491.5
55	91730.5
56	84390.0
57	75401.5
58	67606.0
59	58950.0
60	48508.0
61	40279.0
62	33365.0
63	25452.0
64	18281.5
65	13509.5
66	10080.5
67	7689.5
68	5971.5
69	4542.5
70	3498.0
71	2615.5
72	1866.0
73	1372.0
74	1045.5
75	769.0
76	556.5
77	375.5
78	235.5
79	169.0
80	124.5
81	100.5
82	81.0
83	65.0
84	50.0
85	43.0
86	47.5
87	37.5
88	29.5
89	27.5
90	27.0
91	29.5
92	20.5
93	15.5
94	13.5
95	9.5
96	12.0
97	10.0
98	5.0
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04690316566789685
2	0.05442457421644247
3	0.04588904316696935
4	0.05298790067346185
5	0.04597355337537998
6	0.01757812334940999
7	0.014578010950832805
8	0.02011342960172874
9	0.02024019491434468
10-11	0.021655740905222647
12-13	0.02630380236780702
14-15	0.042445252174236385
16-17	0.02854332289068858
18-19	0.02286001137507405
20-21	0.026324929919909677
22-23	0.04489604821814451
24-25	0.04043813472448404
26-27	0.029895486225258578
28-29	0.01981764387229155
30-31	0.012528638396875149
32-33	0.009507398446195308
34-35	0.00961303620670859
36-37	0.0014789286471859368
38-39	0.0066974340165420285
40-41	0.00382408693058078
42-43	0.20366960226960618
44-45	0.18004899901883648
46-47	0.1853520145966032
48-49	0.18349279001156946
50-51	0.18258430527115524
52-53	0.18558441766973244
54-55	0.18476044313772882
56-57	0.19473264773018256
58-59	0.19181704554001602
60-61	0.19623270392947115
62-63	0.19338048439561256
64-65	0.1948171579385932
66-67	0.18478157068983148
68-69	0.18387308594941726
70-71	0.16959086072802165
72-73	0.1758446161504079
74-75	0.17576010594199726
76-77	0.17335156500229446
78-79	0.19874688262968726
80-81	0.20728241367916037
82-83	0.20495838294786817
84-85	0.20066948987102895
86-87	0.2175504040010513
88-89	0.21558554165550425
90-91	0.2142756334251396
92-93	0.2187758020230054
94-95	0.22889589948017772
96-97	0.22900153724069097
98-99	0.21926173572136645
100-101	0.22900153724069097
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2366578.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.20652129621573
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.44064530142359	46.441977365276394
2	11.804472253276394	13.978034757083929
3	3.9713409952838306	7.0538785563542
4	1.8209995272410961	4.312601891599949
5	1.0109552681897154	2.992757230779793
6	0.6524729036032199	2.317839051743265
7	0.4386579510649256	1.8179987925035914
8	0.3097621380414273	1.4671950898168866
9	0.23661045454368124	1.2607993724262971
>10	1.2272619871946748	12.955499812336912
>50	0.06444353384597226	2.593543814762481
>100	0.020945795387288423	2.1155406137401433
>500	0.0011455127234668207	0.47127689574667003
>1k	2.863781808659397E-4	0.22105675582964798
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	4.225510420531248E-5
9	0.0	0.0	0.0	0.0	4.225510420531248E-5
10-11	0.0	0.0	0.0	0.0	4.225510420531248E-5
12-13	0.0	0.0	0.0	4.225510420531248E-5	4.225510420531248E-5
14-15	0.0	0.0	0.0	4.225510420531248E-5	1.690204168212499E-4
16-17	0.0	0.0	0.0	4.225510420531248E-5	2.1127552102656242E-4
18-19	0.0	0.0	0.0	4.225510420531248E-5	2.9578572943718737E-4
20-21	0.0	0.0	0.0	8.451020841062496E-5	2.9578572943718737E-4
22-23	2.112755210265624E-5	0.0	0.0	2.9578572943718737E-4	3.591683857451561E-4
24-25	4.225510420531248E-5	0.0	0.0	6.338265630796872E-4	4.648061462584373E-4
26-27	4.225510420531248E-5	0.0	0.0	0.0011831429177487495	4.648061462584373E-4
28-29	4.225510420531248E-5	0.0	0.0	0.0023874133876001552	4.648061462584373E-4
30-31	4.225510420531248E-5	0.0	0.0	0.007901704486393433	4.648061462584373E-4
32-33	4.225510420531248E-5	0.0	0.0	0.02381075121969358	5.070612504637497E-4
34-35	4.225510420531248E-5	0.0	0.0	0.04455800738450201	5.493163546690622E-4
36-37	4.225510420531248E-5	0.0	0.0	0.075657764079612	5.493163546690622E-4
38-39	4.225510420531248E-5	0.0	0.0	0.12621599626126837	5.704439067717185E-4
40-41	4.225510420531248E-5	0.0	0.0	0.1859647136075802	6.12699010977031E-4
42-43	4.225510420531248E-5	0.0	0.0	0.2502347271038605	6.760816672849996E-4
44-45	4.225510420531248E-5	0.0	0.0	0.3312166343133419	7.394643235929684E-4
46-47	4.225510420531248E-5	0.0	0.0	0.4253610064827781	7.605918756956247E-4
48-49	4.225510420531248E-5	0.0	0.0	0.5233505931348977	8.451020841062497E-4
50-51	4.225510420531248E-5	0.0	0.0	0.626157261666423	8.451020841062497E-4
52-53	4.225510420531248E-5	0.0	0.0	0.7339289049420725	9.507398446195308E-4
54-55	4.225510420531248E-5	0.0	0.0	0.8643492840717695	0.0010775051572354682
56-57	4.225510420531248E-5	0.0	0.0	1.0171226133260767	0.0010986327093381245
58-59	4.225510420531248E-5	0.0	0.0	1.17813568790042	0.0010986327093381245
60-61	4.225510420531248E-5	0.0	0.0	1.3507900436833267	0.0010986327093381245
62-63	4.225510420531248E-5	2.112755210265624E-5	0.0	1.5289586905650268	0.0011831429177487495
64-65	4.225510420531248E-5	4.225510420531248E-5	0.0	1.731276129500063	0.0011831429177487495
66-67	4.225510420531248E-5	4.225510420531248E-5	0.0	1.9687709426860218	0.0011831429177487495
68-69	4.225510420531248E-5	4.225510420531248E-5	0.0	2.2270552671409947	0.0011831429177487495
70-71	4.225510420531248E-5	4.225510420531248E-5	0.0	2.4996851994736704	0.0012042704698514057
72-73	4.225510420531248E-5	4.225510420531248E-5	0.0	2.7816535098357207	0.0014366735429806243
74-75	4.225510420531248E-5	4.225510420531248E-5	0.0	3.0850451580298643	0.0014366735429806243
76-77	4.225510420531248E-5	4.225510420531248E-5	0.0	3.4313257369923997	0.0014578010950832805
78-79	4.225510420531248E-5	6.338265630796872E-5	0.0	3.8199670579207616	0.0014789286471859368
80-81	4.225510420531248E-5	8.451020841062496E-5	0.0	4.226059736885917	0.0015423113034939056
82-83	4.225510420531248E-5	8.451020841062496E-5	0.0	4.65241373831752	0.0016479490640071868
84-85	4.225510420531248E-5	8.451020841062496E-5	0.0	5.101226327634247	0.0016479490640071868
86-87	4.225510420531248E-5	8.451020841062496E-5	0.0	5.593350398761418	0.0016479490640071868
88-89	4.225510420531248E-5	8.451020841062496E-5	0.0	6.1253210331542	0.0016479490640071868
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2280	0.0	53.916386	1
GTATCAA	4485	0.0	43.494587	1
TCAACGC	5625	0.0	34.51093	4
CAACGCA	5690	0.0	33.94771	5
TATCAAC	5765	0.0	33.426567	2
ATCAACG	5875	0.0	33.04168	3
AACGCAG	6115	0.0	31.732119	6
GTACATG	8285	0.0	26.810764	1
ACGCAGA	7045	0.0	26.673391	7
CGCAGAG	7245	0.0	25.807161	8
TACATGG	8675	0.0	25.551266	2
ACATGGG	8845	0.0	24.307878	3
CATGGGG	7120	0.0	22.531626	4
GCAGAGT	7995	0.0	22.31829	9
GTATAGG	750	0.0	21.516592	1
ATGGGGG	4425	0.0	21.34362	5
TAAGGTA	605	0.0	19.61284	4
GAGTACA	6435	0.0	19.398272	1
AGAGTAC	6830	0.0	19.315931	10-11
CAGAGTA	7405	0.0	18.520998	10-11
>>END_MODULE