FastQCFastQC Report
Mon 15 Aug 2016
SRR2921364_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921364_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2366578
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23350.053.9275781
GTATCAA43000.043.760141
TCAACGC54950.033.679864
TATCAAC57300.032.5470282
CAACGCA57350.032.3264275
ATCAACG57950.032.181963
AACGCAG62550.029.8663836
ACGCAGA73650.024.6551697
CGCAGAG77600.022.7876018
CCCTATA4700.022.2125382
GTACATG77700.022.1992111
TACATGG83450.020.4146272
GCAGAGT84600.019.6659569
ACATGGG83800.019.0269283
GTATATA6900.018.5937351
GGACCGT3357.2577677E-1018.4465836
AGAGTAC72050.018.44018210-11
TAAGTAC15000.018.0565811
GTGTAGC13150.017.7060741
CAGAGTA77150.017.52926410-11
Produced by FastQC (version 0.11.5)