##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921364_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2366578
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.271510594622278	33.0	31.0	34.0	28.0	34.0
2	31.40710426615983	34.0	31.0	34.0	28.0	34.0
3	31.478933295247398	34.0	31.0	34.0	28.0	34.0
4	34.974011420709566	37.0	35.0	37.0	32.0	37.0
5	34.70144022297173	37.0	35.0	37.0	32.0	37.0
6	34.655598082970435	37.0	35.0	37.0	32.0	37.0
7	34.52211758919419	37.0	35.0	37.0	31.0	37.0
8	34.48057828645411	37.0	35.0	37.0	31.0	37.0
9	36.08035906697349	39.0	37.0	39.0	31.0	39.0
10-11	35.927584681341585	39.0	37.0	39.0	31.0	39.0
12-13	35.85938599953181	39.0	37.0	39.0	30.0	39.0
14-15	37.07756093397302	40.0	38.0	41.0	31.0	41.0
16-17	37.0538152556138	40.0	38.0	41.0	31.0	41.0
18-19	37.021770252237616	40.0	38.0	41.0	31.0	41.0
20-21	36.983847986417516	40.0	38.0	41.0	31.0	41.0
22-23	36.871158694114456	40.0	37.0	41.0	30.0	41.0
24-25	36.79881140617381	40.0	37.0	41.0	30.0	41.0
26-27	36.65740765780802	40.0	37.0	41.0	30.0	41.0
28-29	36.504235862921064	40.0	37.0	41.0	30.0	41.0
30-31	36.36353840862207	40.0	36.5	41.0	29.5	41.0
32-33	36.31332730212146	40.0	36.5	41.0	29.5	41.0
34-35	36.44584142166453	40.0	37.0	41.0	30.0	41.0
36-37	36.41979051609539	40.0	37.0	41.0	30.0	41.0
38-39	36.33447957346007	40.0	37.0	41.0	29.0	41.0
40-41	36.20734135954953	40.0	36.0	41.0	28.5	41.0
42-43	36.100365379886064	40.0	36.0	41.0	28.0	41.0
44-45	35.957719965283204	40.0	36.0	41.0	28.0	41.0
46-47	35.7991228262918	40.0	35.5	41.0	27.0	41.0
48-49	35.63661117444682	40.0	35.0	41.0	27.0	41.0
50-51	35.48286386504058	39.5	35.0	41.0	26.0	41.0
52-53	35.31286017194447	39.0	35.0	41.0	26.0	41.0
54-55	35.105094571148726	39.0	35.0	41.0	26.0	41.0
56-57	34.8396968111763	39.0	35.0	41.0	25.0	41.0
58-59	34.61526452962886	38.5	35.0	40.0	24.5	41.0
60-61	34.340821008223685	38.0	34.0	40.0	23.5	41.0
62-63	34.03989114240055	37.5	34.0	40.0	23.0	41.0
64-65	33.72184119855758	37.0	34.0	40.0	22.5	41.0
66-67	33.38660504745671	36.0	34.0	39.0	22.0	41.0
68-69	33.020993603422326	36.0	33.5	39.0	21.5	41.0
70-71	32.64316113814968	35.0	33.0	38.5	20.5	40.0
72-73	32.24912658699607	35.0	33.0	37.5	19.5	40.0
74-75	31.83942173044793	35.0	32.5	37.0	18.0	39.0
76-77	29.102047344308957	33.0	28.5	34.5	10.5	36.5
78-79	30.752549884263267	34.5	31.5	36.0	11.5	37.0
80-81	30.952711890332793	35.0	32.0	35.5	12.5	37.0
82-83	30.791709590810022	35.0	32.0	35.0	9.5	37.0
84-85	30.56702187715765	35.0	32.0	35.0	7.0	36.0
86-87	30.325233945384433	35.0	32.0	35.0	2.0	36.0
88-89	30.04902711848078	35.0	31.0	35.0	2.0	36.0
90-91	29.8692992582539	34.0	31.0	35.0	2.0	35.0
92-93	29.72637538251433	34.0	31.0	35.0	2.0	35.0
94-95	29.630066281356456	34.0	31.0	35.0	2.0	35.0
96-97	29.440547068383125	34.0	31.0	35.0	2.0	35.0
98-99	29.17715367927869	34.0	31.0	35.0	2.0	35.0
100-101	28.08576433990344	33.0	28.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	50251.0
3	16536.0
4	8683.0
5	6237.0
6	5427.0
7	5330.0
8	5622.0
9	6267.0
10	6622.0
11	7127.0
12	7676.0
13	7872.0
14	8840.0
15	9732.0
16	10620.0
17	11496.0
18	10577.0
19	9284.0
20	8987.0
21	8671.0
22	9171.0
23	10481.0
24	12065.0
25	14593.0
26	17570.0
27	20905.0
28	25177.0
29	30379.0
30	36963.0
31	46039.0
32	58561.0
33	78248.0
34	113064.0
35	167699.0
36	292546.0
37	564101.0
38	586745.0
39	70414.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.09846776206939	20.18589122422468	14.348619039357486	25.367021974348447
2	14.907192648936077	21.047812254787416	37.76927695748571	26.2757181387908
3	17.77279233009204	25.10920398251722	29.043420864140824	28.074582823249916
4	13.148572672127647	17.292735850850523	35.516768540683756	34.04192293633807
5	14.282429616605452	37.58458475804849	33.88255134257831	14.250434282767744
6	33.1076609996459	35.712879211073634	17.076108001578664	14.1033517877018
7	28.9313281272118	32.01752069342463	20.7829211268784	18.268230052485176
8	26.925565139640632	34.848358721322576	19.520869928617277	18.70520621041952
9	26.730283134551236	15.958147164386721	19.287764865556937	38.0238048355051
10-11	25.665126807839663	25.660579980594566	27.398461945805202	21.275831265760573
12-13	26.161390010823677	22.93243622683701	27.475780892316166	23.430392870023155
14-15	23.490209401915273	24.493567168430065	24.717657727553547	27.29856570210112
16-17	23.603856716019326	27.709054400603744	25.47394481387221	23.213144069504725
18-19	23.638916237423782	26.976806094896833	26.814419180501737	22.569858487177648
20-21	24.09771480593883	26.005779273337826	26.8895090575542	23.006996863169142
22-23	24.047499073026497	25.668936827416022	26.606072706414025	23.67749139314346
24-25	23.811699980034167	26.014808523404927	26.55390722963987	23.619584266921045
26-27	23.51695859753177	26.28337002937021	26.602362505010007	23.597308868088014
28-29	23.677517679052904	26.427038529126463	26.40400806193125	23.491435729889385
30-31	24.13225931121686	26.00343032876344	26.28569182698921	23.57861853303049
32-33	23.82759450612587	26.09201408735521	26.214318059212623	23.8660733473063
34-35	24.07908774363106	26.224010943628016	26.27489188328572	23.422009429455205
36-37	24.03009434590117	26.11009874047007	26.38277328989471	23.47703362373405
38-39	23.921747590661177	26.259833577885317	26.249227522080986	23.56919130937252
40-41	24.114363926247737	26.01880950600115	26.271063608431653	23.595762959319458
42-43	23.946713241587894	25.99128241546692	26.11108634271149	23.950918000233692
44-45	23.78915612118359	26.016823611965386	26.261860697146037	23.93215956970499
46-47	24.030417110120986	25.98592651895914	26.03224227195672	23.951414098963156
48-49	23.738669381429947	26.24868440264115	26.04518280596394	23.967463409964964
50-51	23.87274500933996	26.203519159853883	26.122405945964104	23.801329884842055
52-53	24.17362037656201	25.972869703375284	26.013054910275223	23.840455009787483
54-55	23.575855595850925	25.955519685280592	26.315325774517994	24.153298944350485
56-57	23.702329551637106	26.077582700614954	26.071370844632817	24.14871690311512
58-59	23.793463378353252	26.071455737926602	26.16444666069406	23.97063422302608
60-61	23.843418099544834	26.132367812419844	26.266052397934235	23.758161690101087
62-63	23.81188951512836	25.995941934415036	26.29896557786651	23.893202972590096
64-65	23.525573922454925	25.80107994916643	26.30556909947947	24.367777028899177
66-67	23.6140955789156	26.02469311146435	26.06692126140852	24.29429004821153
68-69	23.574810784171596	26.023722404836285	26.175944620953366	24.225522190038753
70-71	23.552006202162517	25.83871545233366	26.1659226424797	24.443355703024125
72-73	23.541123069227485	26.096248525194216	25.93551239344046	24.42711601213784
74-75	23.492292931982536	26.122797452250836	26.020600081994584	24.364309533772047
76-77	23.540147144714044	25.73486591076869	26.207171557725562	24.517815386791707
78-79	23.430301390251326	25.757661604032066	26.23327723843994	24.578759767276665
80-81	23.636962821521784	26.00016023110483	25.91649338243423	24.44638356493915
82-83	23.57149634409484	25.92771195342892	26.06473047354015	24.436061228936097
84-85	23.552367207116806	25.9547310221987	25.9366824376917	24.55621933299279
86-87	23.323377084162374	26.140841949372412	25.963227989930132	24.572552976535082
88-89	23.245623976202243	25.876692860778753	26.0384457571745	24.8392374058445
90-91	23.275446780634063	25.810391603705263	26.101212559345218	24.81294905631546
92-93	23.411883848981606	25.629459962826907	26.153108929258035	24.805547258933455
94-95	23.28184784062436	25.577006494300875	26.105475289139747	25.03567037593502
96-97	23.13486874147286	25.46200537834459	26.23708370072536	25.166042179457193
98-99	22.87641202442882	25.39032574436447	26.1855554226851	25.54770680852161
100-101	22.94960361049013	25.539155440930113	26.18822305986968	25.323017888710076
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	60.0
1	44.5
2	45.5
3	63.0
4	61.5
5	69.0
6	125.0
7	302.0
8	469.0
9	496.5
10	410.5
11	318.0
12	318.5
13	343.0
14	375.0
15	435.0
16	481.5
17	518.5
18	594.5
19	668.0
20	753.0
21	846.5
22	979.0
23	1180.5
24	1418.5
25	1773.0
26	2388.0
27	3253.5
28	4309.0
29	6100.0
30	9003.5
31	13104.5
32	18943.5
33	24641.5
34	30087.0
35	36706.0
36	45377.0
37	53643.5
38	59662.5
39	65342.0
40	69325.0
41	72580.5
42	76563.0
43	81537.0
44	86268.5
45	91266.0
46	95435.5
47	98864.5
48	103260.0
49	104602.0
50	103120.5
51	101990.0
52	101196.5
53	100035.5
54	98221.0
55	93049.0
56	85157.5
57	76133.0
58	68063.0
59	59256.5
60	48741.0
61	40445.5
62	33127.0
63	25038.5
64	17663.5
65	12791.0
66	9560.0
67	7137.0
68	5457.5
69	4296.0
70	3229.5
71	2335.5
72	1615.0
73	1142.0
74	855.0
75	585.0
76	400.5
77	233.0
78	121.0
79	69.5
80	41.0
81	33.0
82	20.0
83	10.5
84	8.5
85	3.5
86	2.0
87	3.5
88	2.5
89	1.0
90	1.5
91	1.5
92	0.5
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14231519096349243
2	3.802959378478123E-4
3	0.0057044390677171855
4	0.00392972469109406
5	0.02569110335682999
6	8.451020841062496E-4
7	2.112755210265624E-4
8	0.0016479490640071868
9	0.0
10-11	0.0968486988385762
12-13	2.746581773345311E-4
14-15	0.005239632921458747
16-17	2.112755210265624E-4
18-19	3.380408336424998E-4
20-21	7.394643235929684E-4
22-23	2.3240307312921864E-4
24-25	0.001500056199288593
26-27	0.002725454221242655
28-29	0.006232627870283592
30-31	0.0140498221482664
32-33	0.014472373190319526
34-35	0.011387750583331713
36-37	8.662296362089059E-4
38-39	2.3240307312921864E-4
40-41	0.0048593369836109354
42-43	0.00893695453942359
44-45	0.008514403497370465
46-47	0.008979209643628902
48-49	0.010880689332867965
50-51	0.005598801307203903
52-53	0.001500056199288593
54-55	0.0010775051572354682
56-57	0.005725566619819841
58-59	0.00921161271675812
60-61	0.007901704486393433
62-63	0.018000674391463117
64-65	0.034585802792048266
66-67	0.03623375185605545
68-69	0.026578460545141548
70-71	0.040205731651354826
72-73	0.024867128824826397
74-75	0.02400089918861749
76-77	0.23244532823342395
78-79	0.0649883502677706
80-81	0.052586477183511386
82-83	0.020050046945420775
84-85	0.03133215976823921
86-87	0.0802424428858884
88-89	0.261453457270371
90-91	0.3124553680461831
92-93	0.048910283117649195
94-95	0.005007229848329529
96-97	0.009993332144556402
98-99	0.001225398021954062
100-101	0.0012465255740567182
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2366578.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.66579299959957
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.03121387084812	45.77763040454657
2	12.051006451086604	14.139636997925715
3	3.9824408315995763	7.008991483793216
4	1.8192118255669825	4.2690201752454495
5	1.0385739508114251	3.0464382206539677
6	0.6604183000237241	2.324637796940354
7	0.4657229159769955	1.9125402928713418
8	0.33309558272387363	1.563305320412781
9	0.25036194381039767	1.3218913773502348
>10	1.2798567827234444	13.242968738310887
>50	0.06513401294762404	2.577885175871221
>100	0.021589707632502286	2.1607802022916798
>500	9.399850122552419E-4	0.3460558722040861
>1k	4.3383923642442213E-4	0.3082179415824635
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.225510420531248E-5
2	0.0	0.0	0.0	0.0	4.225510420531248E-5
3	0.0	0.0	0.0	0.0	4.225510420531248E-5
4	0.0	0.0	0.0	0.0	4.225510420531248E-5
5	0.0	0.0	0.0	0.0	4.225510420531248E-5
6	0.0	0.0	0.0	0.0	1.2676531261593743E-4
7	0.0	0.0	0.0	0.0	1.2676531261593743E-4
8	0.0	0.0	0.0	0.0	1.690204168212499E-4
9	0.0	0.0	0.0	4.225510420531248E-5	1.690204168212499E-4
10-11	0.0	0.0	0.0	4.225510420531248E-5	1.690204168212499E-4
12-13	0.0	0.0	0.0	4.225510420531248E-5	2.1127552102656242E-4
14-15	0.0	0.0	0.0	4.225510420531248E-5	2.3240307312921864E-4
16-17	0.0	0.0	0.0	4.225510420531248E-5	2.5353062523187487E-4
18-19	0.0	0.0	0.0	4.225510420531248E-5	2.5353062523187487E-4
20-21	0.0	0.0	0.0	8.451020841062496E-5	2.5353062523187487E-4
22-23	0.0	0.0	0.0	2.3240307312921864E-4	3.1691328153984357E-4
24-25	0.0	0.0	0.0	5.915714588743747E-4	4.2255104205312483E-4
26-27	0.0	0.0	0.0	0.0010986327093381245	4.2255104205312483E-4
28-29	0.0	0.0	0.0	0.0023451582833948427	4.2255104205312483E-4
30-31	0.0	0.0	0.0	0.007986214694804058	4.436785941557811E-4
32-33	0.0	0.0	0.0	0.0240431542928228	4.648061462584373E-4
34-35	0.0	0.0	0.0	0.044938303322349826	4.648061462584373E-4
36-37	0.0	0.0	0.0	0.076333845746897	5.070612504637497E-4
38-39	0.0	0.0	0.0	0.12672305751173213	5.493163546690622E-4
40-41	0.0	0.0	0.0	0.18549990746132178	5.493163546690622E-4
42-43	0.0	0.0	0.0	0.24945300767606224	5.493163546690622E-4
44-45	0.0	0.0	0.0	0.3299067260829772	5.704439067717185E-4
46-47	0.0	0.0	0.0	0.42398771559610543	5.915714588743747E-4
48-49	0.0	0.0	0.0	0.5211533277162215	5.915714588743747E-4
50-51	0.0	0.0	0.0	0.6227345982257926	5.915714588743747E-4
52-53	0.0	0.0	0.0	0.7293019710315908	6.760816672849996E-4
54-55	0.0	4.225510420531248E-5	0.0	0.858665972556155	7.183367714903121E-4
56-57	0.0	4.225510420531248E-5	0.0	1.0100237558195841	7.394643235929684E-4
58-59	0.0	4.225510420531248E-5	0.0	1.1696212844030494	7.817194277982809E-4
60-61	0.0	4.225510420531248E-5	0.0	1.3415995585186713	8.028469799009372E-4
62-63	0.0	4.225510420531248E-5	0.0	1.5184794247221092	8.873571883115621E-4
64-65	0.0	4.225510420531248E-5	0.0	1.718662980894777	8.873571883115621E-4
66-67	0.0	4.225510420531248E-5	0.0	1.9546366103293447	8.873571883115621E-4
68-69	0.0	4.225510420531248E-5	0.0	2.2107447969177434	8.873571883115621E-4
70-71	0.0	4.225510420531248E-5	0.0	2.4825930098226214	9.507398446195308E-4
72-73	0.0	4.225510420531248E-5	0.0	2.764371172215748	0.0010775051572354682
74-75	0.0	4.225510420531248E-5	0.0	3.0681008612435337	0.0010986327093381245
76-77	0.0	4.225510420531248E-5	0.0	3.4153533076027918	0.0011197602614407807
78-79	0.0	4.225510420531248E-5	0.0	3.8052622816573125	0.001140887813543437
80-81	8.451020841062496E-5	4.225510420531248E-5	0.0	4.215052282240434	0.0011831429177487495
82-83	8.451020841062496E-5	4.225510420531248E-5	0.0	4.642652809246092	0.0012676531261593745
84-85	8.451020841062496E-5	4.225510420531248E-5	0.0	5.09186682205277	0.0012676531261593745
86-87	8.451020841062496E-5	4.225510420531248E-5	0.0	5.5846247197430205	0.0012887806782620307
88-89	8.451020841062496E-5	4.225510420531248E-5	0.0	6.118771492002376	0.001309908230364687
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2335	0.0	53.927578	1
GTATCAA	4300	0.0	43.76014	1
TCAACGC	5495	0.0	33.67986	4
TATCAAC	5730	0.0	32.547028	2
CAACGCA	5735	0.0	32.326427	5
ATCAACG	5795	0.0	32.18196	3
AACGCAG	6255	0.0	29.866383	6
ACGCAGA	7365	0.0	24.655169	7
CGCAGAG	7760	0.0	22.787601	8
CCCTATA	470	0.0	22.212538	2
GTACATG	7770	0.0	22.199211	1
TACATGG	8345	0.0	20.414627	2
GCAGAGT	8460	0.0	19.665956	9
ACATGGG	8380	0.0	19.026928	3
GTATATA	690	0.0	18.593735	1
GGACCGT	335	7.2577677E-10	18.446583	6
AGAGTAC	7205	0.0	18.440182	10-11
TAAGTAC	1500	0.0	18.056581	1
GTGTAGC	1315	0.0	17.706074	1
CAGAGTA	7715	0.0	17.529264	10-11
>>END_MODULE