FastQCFastQC Report
Mon 15 Aug 2016
SRR2921363_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921363_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1637136
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22850.13957301042796688No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22580.1379237888605467No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24250.064.379531
GTATCAA46000.042.398221
TCAACGC56950.033.8305634
ATCAACG57500.033.5069663
TATCAAC58500.032.691842
CAACGCA59000.032.571685
AACGCAG61350.031.2361836
GTGGTAT9050.028.3144951
ACGCAGA70300.026.5879677
GTACATG64350.026.1784231
CGCAGAG71100.026.02438
TACATGG66900.024.33092
GCAGAGT74050.023.6420619
ACATGGG68200.023.4488983
GAGTACT32950.022.31825812-13
CATGGGG52650.021.6316594
CAGAGTA71450.021.28187210-11
TGGTATC11600.021.2733332
GTACTTT33400.021.2359714-15
ATGGGGG36300.019.7381955
Produced by FastQC (version 0.11.5)