##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921363_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1637136
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.633478220502145	31.0	31.0	34.0	27.0	34.0
2	30.69042339793395	33.0	31.0	34.0	27.0	34.0
3	30.719077706433673	33.0	31.0	34.0	27.0	34.0
4	34.193597844039836	37.0	35.0	37.0	30.0	37.0
5	34.20139744040813	37.0	35.0	37.0	30.0	37.0
6	34.24900802376834	37.0	35.0	37.0	30.0	37.0
7	34.18350094311041	37.0	35.0	37.0	30.0	37.0
8	34.15354802533204	37.0	35.0	37.0	30.0	37.0
9	35.73316755602467	39.0	37.0	39.0	30.0	39.0
10-11	35.63542735606572	39.0	37.0	39.0	30.0	39.0
12-13	35.523978459944686	39.0	37.0	39.0	30.0	39.0
14-15	36.66931244563677	40.0	37.0	41.0	30.0	41.0
16-17	36.61182943872714	40.0	37.0	41.0	30.0	41.0
18-19	36.585807165684464	40.0	37.0	41.0	30.0	41.0
20-21	36.53358853510032	40.0	37.0	41.0	29.0	41.0
22-23	36.42117698224216	40.0	37.0	41.0	29.0	41.0
24-25	36.318771317715814	40.0	37.0	41.0	28.5	41.0
26-27	36.16447564527321	40.0	36.0	41.0	28.0	41.0
28-29	36.025811539175734	40.0	36.0	41.0	27.0	41.0
30-31	35.85232717379619	40.0	36.0	41.0	27.0	41.0
32-33	35.64806466903177	40.0	35.5	41.0	26.5	41.0
34-35	35.47909917074696	39.0	35.0	41.0	25.5	41.0
36-37	35.2981862838518	39.0	35.0	41.0	25.0	41.0
38-39	35.50887892026075	39.5	35.0	41.0	26.0	41.0
40-41	35.609554429198305	40.0	35.0	41.0	26.0	41.0
42-43	35.40847003547659	40.0	35.0	41.0	25.0	41.0
44-45	35.24841522023827	40.0	35.0	41.0	24.0	41.0
46-47	35.04925125340839	40.0	35.0	41.0	23.0	41.0
48-49	34.87402909715503	40.0	35.0	41.0	22.5	41.0
50-51	33.143352171108575	37.0	32.5	39.5	18.5	40.0
52-53	33.2990087567557	37.5	33.5	39.5	18.5	40.0
54-55	34.15096332864222	39.0	34.0	40.5	19.0	41.0
56-57	34.1624516839163	39.0	34.0	41.0	18.0	41.0
58-59	33.983046307698324	38.5	34.0	41.0	18.0	41.0
60-61	33.73642812814573	38.0	34.0	40.5	14.5	41.0
62-63	33.45184731140235	37.0	34.0	40.0	13.0	41.0
64-65	33.16275526284927	37.0	33.5	40.0	9.5	41.0
66-67	32.82608744783573	36.0	33.0	39.5	7.5	41.0
68-69	32.46226306183481	36.0	33.0	39.0	4.5	41.0
70-71	32.08093310512994	35.0	33.0	39.0	2.0	41.0
72-73	31.719063962920615	35.0	32.5	37.5	2.0	40.0
74-75	31.31695137117503	35.0	32.0	37.0	2.0	39.0
76-77	30.93906279014083	35.0	32.0	37.0	2.0	39.0
78-79	30.587515331652348	35.0	32.0	36.0	2.0	39.0
80-81	30.236759194104827	35.0	31.0	36.0	2.0	37.0
82-83	29.928310781755457	35.0	31.0	35.0	2.0	37.0
84-85	29.682477204093	35.0	31.0	35.0	2.0	36.5
86-87	29.44653284760704	35.0	31.0	35.0	2.0	36.0
88-89	29.228546376110476	35.0	31.0	35.0	2.0	36.0
90-91	28.96541826702241	35.0	30.0	35.0	2.0	35.5
92-93	28.768206795281515	34.5	30.0	35.0	2.0	35.0
94-95	28.555354900264852	34.0	30.0	35.0	2.0	35.0
96-97	28.295118120913596	34.0	30.0	35.0	2.0	35.0
98-99	28.049817791557942	34.0	29.5	35.0	2.0	35.0
100-101	26.99869497708193	33.5	26.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	49141.0
3	8683.0
4	6058.0
5	4834.0
6	4240.0
7	4465.0
8	4763.0
9	5208.0
10	5578.0
11	6195.0
12	6231.0
13	6636.0
14	7413.0
15	8223.0
16	9281.0
17	10255.0
18	9038.0
19	7418.0
20	7175.0
21	7428.0
22	7955.0
23	9037.0
24	10600.0
25	12432.0
26	14761.0
27	17115.0
28	20446.0
29	23566.0
30	28291.0
31	34641.0
32	43302.0
33	55907.0
34	79608.0
35	116090.0
36	188377.0
37	343618.0
38	379561.0
39	73558.0
40	8.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.19941430157724	18.894029775040227	13.997116730844564	23.909439192537967
2	16.7265928727847	20.266425617246036	38.58917636762237	24.417805142346896
3	19.5041418274152	24.13919962355357	29.68194554360148	26.67471300542975
4	14.165088856483852	16.641751729887044	35.73413093726203	33.45902847636707
5	14.681617682107792	36.98830212800804	33.62029032624731	14.709789863636862
6	32.811274324129485	34.48607448932463	17.452830188679243	15.249820997866637
7	28.284926378501616	32.38594501999528	20.92100488366325	18.40812371783985
8	26.7713884753061	33.61793666959721	20.26471470526601	19.345960149830674
9	27.120998841625244	15.77075910203962	19.882439625214445	37.22580243112069
10-11	25.584582289354564	25.507662361227958	27.35771187957724	21.55004346984024
12-13	26.310674836430092	23.386350034872308	27.121795848988704	23.1811792797089
14-15	23.946703691063025	24.95073785511292	24.912483114595865	26.19007533922819
16-17	23.45169538904283	27.622086646768235	25.972492507538288	22.95372545665065
18-19	23.30538647767281	26.77912615798196	27.643681408900328	22.2718059554449
20-21	24.028278914264188	25.7648867703939	27.748791544431118	22.458042770910794
22-23	24.02377104556117	25.516626684645587	27.300280103314645	23.159322166478596
24-25	23.761056052367525	25.94232336898402	27.168312318125874	23.128308260522576
26-27	23.198240033434722	26.241215137032253	27.340461022765183	23.220083806767843
28-29	23.29883907199073	26.490963432172894	27.157724185205957	23.052473310630415
30-31	23.947370430612512	25.859629027208236	27.110581659068522	23.082418883110734
32-33	23.56701360932359	26.004419151039176	26.83821777605644	23.590349463580797
34-35	23.920397292696702	26.26899115108039	26.898992931799086	22.911618624423824
36-37	23.82415614330484	26.09984109206978	27.031756847349737	23.044245917275642
38-39	23.601039450366795	26.187967274662054	26.989362982347792	23.22163029262336
40-41	23.8600538888614	25.896737653116265	26.99140478158274	23.251803676439597
42-43	23.6409988469851	25.945834928791307	26.698829875053832	23.714336349169766
44-45	23.514804372000736	26.071105295594766	26.79698741020954	23.61710292219496
46-47	23.663071124050045	26.025712000984193	26.456203940621677	23.85501293434409
48-49	23.377113404144044	26.320402304566443	26.633979777665097	23.668504513624416
50-51	23.653376363026005	26.17071130885076	26.76278780263668	23.413124525486555
52-53	23.803958400469085	26.031442331498063	26.614830980100884	23.54976828793197
54-55	23.262291715694392	26.00329466301926	26.852574693768634	23.88183892751772
56-57	23.392662138603775	26.116049567108046	26.52136547050067	23.96992282378751
58-59	23.457845790546937	26.0585568560097	26.770418036346545	23.71317931709682
60-61	23.576118524086567	26.00085880809312	26.98655992879789	23.43646273902242
62-63	23.519373097473622	25.862759176657896	27.061737191364543	23.556130534503943
64-65	23.216998698641962	25.758411388290714	26.976338054377912	24.04825185868941
66-67	23.346429367105962	26.065320217305615	26.592425885711346	23.995824529877076
68-69	23.30447555657563	25.95749368831765	26.85101369443807	23.887017060668654
70-71	23.217461279090404	25.7768047829732	26.795848555500477	24.209885382435914
72-73	23.18103177629119	26.12751274925536	26.644031243249	24.04742423120445
74-75	23.251337192935214	26.185420007099058	26.632079166717666	23.93116363324806
76-77	23.1732557506207	25.78175823005665	26.71000911237845	24.334976906944195
78-79	23.13110500190071	25.946759272012287	26.72730578312058	24.194829942966418
80-81	23.412405312740763	26.115394257437924	26.451182590186107	24.021017839635206
82-83	23.397996767083388	25.99427796410122	26.74162592871361	23.866099340101787
84-85	23.347677974038852	26.168664815942748	26.476360419810298	24.0072967902081
86-87	23.13755381985101	26.38512743040728	26.41405583948253	24.06326291025918
88-89	23.02735780035571	26.074368401235493	26.5935757995047	24.304697998904096
90-91	23.215808096840544	25.874827159016156	26.719739090852602	24.189625653290705
92-93	23.41528508107979	25.70800744628125	26.692701915785754	24.1840055568532
94-95	23.27879834218199	25.73671530493497	26.642195981227236	24.342290371655807
96-97	23.24325416580176	25.604196632333593	26.722669934034197	24.42987926783045
98-99	22.944720593678163	25.637652680151024	26.662342023743086	24.75528470242773
100-101	23.220937662362314	25.695197734145403	26.744205626733457	24.339658976758823
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	156.0
1	128.5
2	118.0
3	132.5
4	111.0
5	126.0
6	212.5
7	425.0
8	682.0
9	792.0
10	714.0
11	613.5
12	646.5
13	698.0
14	729.0
15	805.5
16	924.5
17	1032.5
18	1134.5
19	1242.5
20	1362.5
21	1517.0
22	1686.0
23	1947.0
24	2249.0
25	2575.5
26	3197.5
27	4078.0
28	5228.0
29	6991.5
30	9440.0
31	12479.5
32	17170.0
33	21238.5
34	24299.5
35	28477.0
36	33420.5
37	38293.0
38	41434.0
39	44328.0
40	47014.0
41	48816.5
42	51043.5
43	53816.0
44	56511.5
45	60394.0
46	63146.5
47	65036.5
48	67718.0
49	68964.5
50	69411.5
51	68754.0
52	67426.0
53	66395.0
54	64814.0
55	61428.0
56	56621.0
57	51043.0
58	45344.5
59	38835.5
60	32073.0
61	26832.5
62	22294.5
63	17368.5
64	12666.0
65	9308.0
66	7046.0
67	5515.5
68	4372.5
69	3460.5
70	2685.0
71	1940.0
72	1450.5
73	1166.0
74	922.5
75	721.5
76	570.0
77	421.0
78	269.0
79	176.5
80	126.5
81	84.0
82	61.5
83	51.5
84	47.0
85	36.0
86	24.5
87	22.0
88	17.5
89	17.0
90	22.5
91	15.5
92	7.5
93	8.5
94	7.0
95	8.0
96	10.5
97	7.0
98	4.5
99	2.0
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04880474193958229
2	0.05845574222300407
3	0.04770526089463551
4	0.055096216807888905
5	0.04703335581161247
6	0.017347367598049276
7	0.01527057006870535
8	0.021684209497561596
9	0.0219896208989357
10-11	0.022539361421409092
12-13	0.027517567263807042
14-15	0.04504818170268078
16-17	0.029991399614937308
18-19	0.02434128868951633
20-21	0.026845662180784005
22-23	0.047369308353123994
24-25	0.042879760752924614
26-27	0.0313962920612582
28-29	0.02089013985398892
30-31	0.013651889641422584
32-33	0.010383987646719637
34-35	0.009559376863009549
36-37	0.0014965158667331242
38-39	0.007146626792154102
40-41	0.003848183657313748
42-43	0.21943809188729588
44-45	0.1950357209175047
46-47	0.20202964200897178
48-49	0.19848686975303212
50-51	0.19702089502643638
52-53	0.2027626293722696
54-55	0.2004415027218264
56-57	0.21207767711417988
58-59	0.20709947127178194
60-61	0.2123525473754166
62-63	0.2109171137889583
64-65	0.21122252519033238
66-67	0.20206018314910917
68-69	0.19918931597619258
70-71	0.1836133345061131
72-73	0.19051563217716794
74-75	0.19033238533634347
76-77	0.18904965745057223
78-79	0.21604802533204326
80-81	0.22395818062763265
82-83	0.22151488941663977
84-85	0.21629235445314257
86-87	0.23171563022253497
88-89	0.2309521017190997
90-91	0.23031073777621405
92-93	0.23440325055462707
94-95	0.24588671924629354
96-97	0.24536751986395755
98-99	0.235808143000948
100-101	0.24564239012519426
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1637136.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.21763086680068
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.84578197078824	54.19633778546306
2	10.770635931058933	14.264119885671212
3	3.1949472270118866	6.346855083515446
4	1.3696392113668072	3.6277705487593295
5	0.7415937106061268	2.4553289291028753
6	0.45883080274529325	1.82296132359034
7	0.3353041536393612	1.5542132671657372
8	0.2306804592001091	1.2220090796397518
9	0.16985789389777137	1.0122828586141337
>10	0.8325464197268158	9.3498399782398
>50	0.03464479747506605	1.536869366825548
>100	0.014057827479554275	1.7418200860275874
>500	0.0012021709408172655	0.5195751345443123
>1k	2.774240632655228E-4	0.35001667284092974
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2285	0.13957301042796688	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2258	0.1379237888605467	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.221645605496428E-4
2	0.0	0.0	0.0	0.0	1.221645605496428E-4
3	0.0	0.0	0.0	0.0	1.221645605496428E-4
4	0.0	0.0	0.0	6.10822802748214E-5	1.221645605496428E-4
5	0.0	0.0	0.0	6.10822802748214E-5	1.221645605496428E-4
6	0.0	0.0	0.0	6.10822802748214E-5	1.221645605496428E-4
7	0.0	0.0	0.0	6.10822802748214E-5	1.221645605496428E-4
8	0.0	0.0	0.0	6.10822802748214E-5	1.832468408244642E-4
9	0.0	0.0	0.0	6.10822802748214E-5	1.832468408244642E-4
10-11	0.0	0.0	0.0	6.10822802748214E-5	1.832468408244642E-4
12-13	0.0	0.0	0.0	6.10822802748214E-5	1.832468408244642E-4
14-15	3.05411401374107E-5	0.0	0.0	6.10822802748214E-5	2.748702612366963E-4
16-17	6.10822802748214E-5	0.0	0.0	6.10822802748214E-5	3.664936816489284E-4
18-19	6.10822802748214E-5	0.0	0.0	6.10822802748214E-5	3.664936816489284E-4
20-21	6.10822802748214E-5	0.0	0.0	6.10822802748214E-5	3.664936816489284E-4
22-23	6.10822802748214E-5	0.0	0.0	2.443291210992856E-4	3.9703482178633906E-4
24-25	6.10822802748214E-5	0.0	0.0	0.0012216456054964279	4.2757596192374977E-4
26-27	6.10822802748214E-5	0.0	0.0	0.0025654557715424986	4.2757596192374977E-4
28-29	6.10822802748214E-5	0.0	0.0	0.006474721709131068	4.2757596192374977E-4
30-31	6.10822802748214E-5	0.0	0.0	0.01890496574505722	4.2757596192374977E-4
32-33	6.10822802748214E-5	0.0	0.0	0.051095327449888095	5.497405224733925E-4
34-35	6.10822802748214E-5	0.0	0.0	0.09812868326150057	6.719050830230354E-4
36-37	6.10822802748214E-5	3.05411401374107E-5	0.0	0.16031044458126875	7.329873632978568E-4
38-39	6.10822802748214E-5	6.10822802748214E-5	0.0	0.24704728257151515	7.329873632978568E-4
40-41	6.10822802748214E-5	6.10822802748214E-5	0.0	0.3441070259282063	7.940696435726781E-4
42-43	6.10822802748214E-5	1.221645605496428E-4	0.0	0.4528334848173884	7.940696435726781E-4
44-45	6.10822802748214E-5	1.221645605496428E-4	0.0	0.6049894449819685	7.940696435726781E-4
46-47	6.10822802748214E-5	1.221645605496428E-4	0.0	0.7776690513188886	7.940696435726781E-4
48-49	6.10822802748214E-5	1.221645605496428E-4	0.0	0.9371548851164473	9.16234204122321E-4
50-51	6.10822802748214E-5	1.221645605496428E-4	0.0	1.089066516159928	9.16234204122321E-4
52-53	6.10822802748214E-5	1.221645605496428E-4	0.0	1.2538359672012587	9.773164843971424E-4
54-55	6.10822802748214E-5	1.221645605496428E-4	0.0	1.4567513022742156	0.0010383987646719638
56-57	6.10822802748214E-5	1.221645605496428E-4	0.0	1.7051423950117766	0.0010383987646719638
58-59	6.10822802748214E-5	1.221645605496428E-4	0.0	1.9561294846610178	0.0010689399048093744
60-61	6.10822802748214E-5	1.221645605496428E-4	0.0	2.200519688040578	0.001099481044946785
62-63	6.10822802748214E-5	1.221645605496428E-4	0.0	2.4510181194476206	0.001099481044946785
64-65	6.10822802748214E-5	1.221645605496428E-4	0.0	2.7529783719862	0.001099481044946785
66-67	6.10822802748214E-5	1.832468408244642E-4	0.0	3.1224040030883202	0.001099481044946785
68-69	6.10822802748214E-5	1.832468408244642E-4	0.0	3.518766919791636	0.001099481044946785
70-71	6.10822802748214E-5	1.832468408244642E-4	0.0	3.898851408801712	0.0011605633252216065
72-73	6.10822802748214E-5	1.832468408244642E-4	0.0	4.285563325221607	0.0014354335864583028
74-75	6.10822802748214E-5	1.832468408244642E-4	0.0	4.726149812843893	0.0014659747265957136
76-77	6.10822802748214E-5	1.832468408244642E-4	0.0	5.2589400025410225	0.0014659747265957136
78-79	6.10822802748214E-5	1.832468408244642E-4	0.0	5.849727817359096	0.0014659747265957136
80-81	6.10822802748214E-5	1.832468408244642E-4	0.0	6.431780866097869	0.0014659747265957136
82-83	6.10822802748214E-5	1.832468408244642E-4	0.0	7.013436880014855	0.0014659747265957136
84-85	6.10822802748214E-5	2.443291210992856E-4	0.0	7.63769778442353	0.0014659747265957136
86-87	6.10822802748214E-5	2.443291210992856E-4	0.0	8.339197232239716	0.0014659747265957136
88-89	6.10822802748214E-5	2.443291210992856E-4	0.0	9.105627143988038	0.0014659747265957136
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2425	0.0	64.37953	1
GTATCAA	4600	0.0	42.39822	1
TCAACGC	5695	0.0	33.830563	4
ATCAACG	5750	0.0	33.506966	3
TATCAAC	5850	0.0	32.69184	2
CAACGCA	5900	0.0	32.57168	5
AACGCAG	6135	0.0	31.236183	6
GTGGTAT	905	0.0	28.314495	1
ACGCAGA	7030	0.0	26.587967	7
GTACATG	6435	0.0	26.178423	1
CGCAGAG	7110	0.0	26.0243	8
TACATGG	6690	0.0	24.3309	2
GCAGAGT	7405	0.0	23.642061	9
ACATGGG	6820	0.0	23.448898	3
GAGTACT	3295	0.0	22.318258	12-13
CATGGGG	5265	0.0	21.631659	4
CAGAGTA	7145	0.0	21.281872	10-11
TGGTATC	1160	0.0	21.273333	2
GTACTTT	3340	0.0	21.23597	14-15
ATGGGGG	3630	0.0	19.738195	5
>>END_MODULE