FastQCFastQC Report
Mon 15 Aug 2016
SRR2921363_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921363_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1637136
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20910.12772304805465154No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20540.12546300368448315No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25050.062.421621
GTATCAA43600.041.2052151
TCAACGC59300.030.0077194
ATCAACG59950.029.6823653
TATCAAC61150.029.410282
CAACGCA61000.029.232165
AACGCAG64100.027.6675346
GTGGTAT9600.025.7441731
GTACATG64750.024.369421
ACGCAGA73250.023.9503467
TAAGGTG8000.023.7754945
TACATGG67850.023.1491262
CGCAGAG75300.022.8563378
ACATGGG67800.022.5362973
TGGTATC11200.021.1840212
CATGGGG50400.021.089874
GCAGAGT78700.020.6002589
ATGGGGG32950.020.0594445
CAGAGTA75950.019.56209210-11
GAGTACT36250.019.43666612-13
Produced by FastQC (version 0.11.5)