##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921363_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1637136
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.475052164267353	33.0	31.0	34.0	30.0	34.0
2	31.602825910614634	34.0	31.0	34.0	29.0	34.0
3	31.657109733094867	34.0	31.0	34.0	28.0	34.0
4	35.149658305724145	37.0	35.0	37.0	33.0	37.0
5	34.892793268244056	37.0	35.0	37.0	32.0	37.0
6	34.85231220863752	37.0	35.0	37.0	32.0	37.0
7	34.73055628854292	37.0	35.0	37.0	32.0	37.0
8	34.69743808700071	37.0	35.0	37.0	32.0	37.0
9	36.318324806247006	39.0	37.0	39.0	32.0	39.0
10-11	36.17426255363024	39.0	37.0	39.0	32.0	39.0
12-13	36.11208323560169	39.0	37.0	39.0	32.0	39.0
14-15	37.344264312799915	40.0	38.0	41.0	32.0	41.0
16-17	37.314233515114196	40.0	38.0	41.0	32.0	41.0
18-19	37.2928754849933	40.0	38.0	41.0	31.5	41.0
20-21	37.26278604831853	40.0	38.0	41.0	31.0	41.0
22-23	37.153608496789516	40.0	38.0	41.0	31.0	41.0
24-25	37.07598116466805	40.0	37.5	41.0	31.0	41.0
26-27	36.92838805084	40.0	37.0	41.0	30.5	41.0
28-29	36.772770863263645	40.0	37.0	41.0	30.0	41.0
30-31	36.61178607030815	40.0	37.0	41.0	30.0	41.0
32-33	36.531430498138214	40.0	36.5	41.0	30.0	41.0
34-35	36.63957117795956	40.0	37.0	41.0	30.0	41.0
36-37	36.59727841792007	40.0	37.0	41.0	30.0	41.0
38-39	36.49771766059753	40.0	37.0	41.0	30.0	41.0
40-41	36.355986918618854	40.0	36.0	41.0	29.5	41.0
42-43	36.22146388571261	40.0	36.0	41.0	29.0	41.0
44-45	36.04782162263856	40.0	36.0	41.0	28.0	41.0
46-47	35.83942995572757	40.0	35.5	41.0	27.5	41.0
48-49	35.648038709062654	40.0	35.0	41.0	27.0	41.0
50-51	35.47366681815072	40.0	35.0	41.0	26.0	41.0
52-53	35.29977411772754	39.0	35.0	41.0	26.0	41.0
54-55	35.085272390320654	39.0	35.0	41.0	25.5	41.0
56-57	34.82051704928607	39.0	35.0	41.0	24.0	41.0
58-59	34.59873950606425	38.5	35.0	40.5	24.0	41.0
60-61	34.314474179298486	38.0	34.0	40.0	23.0	41.0
62-63	34.01216667399654	37.5	34.0	40.0	22.5	41.0
64-65	33.69999499125302	37.0	34.0	40.0	22.0	41.0
66-67	33.36818657704674	36.5	34.0	39.0	21.5	41.0
68-69	33.005629037538725	36.0	33.5	39.0	20.5	41.0
70-71	32.62361862423158	35.0	33.0	39.0	19.5	40.0
72-73	32.22710116935917	35.0	33.0	37.5	18.0	40.0
74-75	31.822462825324223	35.0	33.0	37.0	14.5	39.0
76-77	29.07967175604226	33.0	28.5	34.5	7.5	36.5
78-79	30.72383296195307	34.5	31.5	36.0	8.0	37.5
80-81	30.91296691295042	35.0	32.0	36.0	7.0	37.0
82-83	30.747477912647454	35.0	32.0	35.0	6.0	37.0
84-85	30.519159678853804	35.0	32.0	35.0	2.0	36.5
86-87	30.277459539097546	35.0	32.0	35.0	2.0	36.0
88-89	29.9957993715855	35.0	31.5	35.0	2.0	36.0
90-91	29.81196430840199	35.0	31.0	35.0	2.0	35.0
92-93	29.663074723175107	34.0	31.0	35.0	2.0	35.0
94-95	29.562592234243215	34.0	31.0	35.0	2.0	35.0
96-97	29.375952272749483	34.0	31.0	35.0	2.0	35.0
98-99	29.115625702446223	34.0	31.0	35.0	2.0	35.0
100-101	28.03101208451833	33.0	28.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	29565.0
3	10450.0
4	5587.0
5	3994.0
6	3611.0
7	3572.0
8	3868.0
9	4041.0
10	4449.0
11	4801.0
12	5331.0
13	5899.0
14	6701.0
15	7803.0
16	8875.0
17	10427.0
18	9296.0
19	7563.0
20	6800.0
21	6479.0
22	6674.0
23	7384.0
24	8440.0
25	10076.0
26	12060.0
27	14493.0
28	17257.0
29	20538.0
30	24908.0
31	30993.0
32	39202.0
33	51914.0
34	76829.0
35	114449.0
36	194405.0
37	379348.0
38	420467.0
39	58580.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.710779335508015	20.0869096008007	14.441945659169495	24.760365404521796
2	15.919566558550635	21.336424108042735	37.0834936749067	25.66051565849994
3	18.33616672174619	24.955788756080384	29.349729723455997	27.35831479871743
4	13.808829763709687	17.575718333054375	34.769958718527306	33.84549318470863
5	14.582232293911387	37.38955747227213	33.416162046934375	14.612048186882106
6	33.034312105595255	34.889339137839094	17.106086973518725	14.970261783046926
7	28.339568819920164	32.42414339684876	20.786740250498585	18.44954753273249
8	27.005765051255082	33.91918421898155	19.831641933998164	19.24340879576521
9	27.011256242609043	15.699673087635968	19.74588549760069	37.543185172154296
10-11	25.600066527985167	25.759568595366765	27.202577714837084	21.43778716181098
12-13	26.254969055641208	23.464235015869214	27.092836741325684	23.187959187163894
14-15	23.811446569156864	25.112215277404477	24.836838864481575	26.239499288957084
16-17	23.384959137419152	27.811323877985515	25.87437127149374	22.92934571310159
18-19	23.40626584327451	26.878317543506014	27.54619364375462	22.169222969464855
20-21	23.966977485843326	25.894196871284457	27.62005722835543	22.518768414516785
22-23	23.928300156890366	25.65467444409594	27.195357975433236	23.221667423580467
24-25	23.715041399046495	25.99136285477807	27.122437947242556	23.171157798932878
26-27	23.200209151221095	26.352320851655954	27.269587005747383	23.17788299137557
28-29	23.26093383863929	26.707331823279752	27.134416760128723	22.89731757795224
30-31	23.936899255257504	25.992890719477884	26.976167079394298	23.094042945870317
32-33	23.524274691640855	26.101937210197384	26.823854993310487	23.549933104851274
34-35	23.812720880436675	26.380844454354683	26.907994611815518	22.89844005339312
36-37	23.73278497315566	26.287937348648406	26.982020598934657	22.997257079261278
38-39	23.63267870335559	26.276965195568962	26.96682952245495	23.1235265786205
40-41	23.86177638842863	26.06307353284781	26.923855759495314	23.15129431922825
42-43	23.648405539119775	26.100893640910172	26.54856931321781	23.702131506752245
44-45	23.501151797151753	26.205477435343894	26.696587125161958	23.5967836423424
46-47	23.621133808069622	26.143065977022307	26.41756552771905	23.818234687189026
48-49	23.387502477945898	26.42444853676722	26.558112527608667	23.62993645767821
50-51	23.616213057909142	26.311293228595417	26.58669909107208	23.48579462242336
52-53	23.82375038940572	26.097085718125236	26.432707637238796	23.64645625523025
54-55	23.135860897440207	26.144797916833372	26.785769914661266	23.933571271065155
56-57	23.408624919862763	26.23376218780839	26.32148172718307	24.036131165145775
58-59	23.38896906665445	26.189540237784342	26.62231690834536	23.799173787215846
60-61	23.554017121375576	26.187128058457514	26.781929093021578	23.476925727145336
62-63	23.407642728411275	26.103949659406787	26.78739652381098	23.701011088370958
64-65	23.120059612318293	25.902853796145546	26.73520209437456	24.241884497161603
66-67	23.259597461595032	26.216599110501154	26.357933920934997	24.16586950696882
68-69	23.19967006781939	26.1123296877864	26.540233396468505	24.147766847925702
70-71	23.12784227359889	25.943411791593284	26.480059934468063	24.44868600033976
72-73	23.00902636424207	26.24293130315747	26.36619721752019	24.381845115080264
74-75	23.032089653687127	26.262744445140275	26.333739119012307	24.371426782160295
76-77	22.8987277634139	25.835908881469678	26.511444924601115	24.75391843051531
78-79	22.816113488564284	25.96598594962869	26.55997569639071	24.65792486541632
80-81	23.026209441461525	26.124583387190864	26.266767261834374	24.58243990951324
82-83	22.933393775957438	26.030966255176214	26.530381582757723	24.505258386108622
84-85	22.788503281298354	26.048018985429454	26.354625850111525	24.808851883160663
86-87	22.532338459008507	26.30615463100427	26.281273602971538	24.880233307015686
88-89	22.479988731221255	25.976004850474332	26.370871431808578	25.17313498649584
90-91	22.53019372147077	25.77608388857262	26.46565707383942	25.22806531611719
92-93	22.60313529588278	25.57296368026607	26.543003520806124	25.28089750304503
94-95	22.39958388577506	25.58228961171326	26.540293045327555	25.477833457184122
96-97	22.224329781027695	25.410592162935675	26.63092979996518	25.73414825607145
98-99	21.93958392634844	25.476600896455594	26.493787237785515	26.090027939410447
100-101	21.912014518258076	25.515893546619623	26.574251837535755	25.99784009758654
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	154.0
1	108.5
2	84.0
3	101.5
4	94.0
5	113.5
6	184.0
7	378.0
8	591.5
9	666.5
10	586.0
11	470.0
12	443.0
13	450.5
14	512.5
15	630.5
16	710.5
17	745.0
18	840.5
19	937.5
20	1015.5
21	1169.0
22	1382.0
23	1623.0
24	1925.5
25	2347.0
26	2957.5
27	3825.5
28	5070.5
29	6985.0
30	9533.0
31	12758.5
32	17453.0
33	21287.5
34	24115.5
35	28044.0
36	33251.0
37	37940.5
38	41030.0
39	44024.5
40	46313.0
41	48423.5
42	50853.5
43	53692.5
44	56775.0
45	60466.5
46	63190.0
47	65474.5
48	68155.0
49	69796.0
50	70239.0
51	69507.5
52	68753.5
53	67750.0
54	66045.5
55	62556.0
56	57372.5
57	51393.0
58	45517.5
59	39099.0
60	32388.0
61	26944.5
62	22278.0
63	17157.0
64	12318.0
65	9080.0
66	6872.0
67	5309.5
68	4136.5
69	3283.5
70	2521.0
71	1802.5
72	1350.0
73	1079.5
74	863.5
75	672.0
76	479.5
77	302.0
78	186.5
79	104.5
80	58.0
81	36.0
82	22.0
83	14.0
84	10.5
85	7.5
86	8.5
87	7.5
88	2.0
89	1.5
90	1.5
91	0.5
92	0.0
93	1.0
94	1.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15661496662464205
2	3.664936816489284E-4
3	0.006657968549955533
4	0.005008746982535355
5	0.025959969116799094
6	0.0010383987646719636
7	6.108228027482139E-5
8	0.001832468408244642
9	0.0
10-11	0.1059166739965403
12-13	2.4432912109928555E-4
14-15	0.006352557148581425
16-17	1.5270570068705349E-4
18-19	3.664936816489284E-4
20-21	6.413639428856247E-4
22-23	3.359525415115177E-4
24-25	0.0012827278857712493
26-27	0.0026265380518173203
28-29	0.00659688626968071
30-31	0.015973016291865795
32-33	0.015026240947606063
34-35	0.013499183940735527
36-37	7.635285034352675E-4
38-39	1.5270570068705349E-4
40-41	0.00479495900157348
42-43	0.007635285034352675
44-45	0.007879614155451959
46-47	0.00958991800314696
48-49	0.011941585793727583
50-51	0.005955522326795086
52-53	0.0015881392871453562
54-55	7.940696435726781E-4
56-57	0.00613876916761955
58-59	0.009070718620810977
60-61	0.0072077090724289245
62-63	0.017469532158598918
64-65	0.034297700374312214
66-67	0.03643558018393096
68-69	0.02663187419982213
70-71	0.04165811514742819
72-73	0.02629592165831061
74-75	0.02403587728814222
76-77	0.24362667487612513
78-79	0.07116085652016693
80-81	0.05341645410033131
82-83	0.019668494248492492
84-85	0.03130466864084597
86-87	0.08255270179142112
88-89	0.2630202988633809
90-91	0.3211095474047361
92-93	0.052714007877170864
94-95	0.00522253496349723
96-97	0.01044506992699446
98-99	0.0013438101660460707
100-101	0.001527057006870535
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1637136.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.70187963464357
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.38448674885427	53.471137525004686
2	11.088128604155925	14.57021781847402
3	3.211147694588471	6.329353179567448
4	1.4248149294656305	3.7445207598957606
5	0.7571731722346484	2.4873850312371064
6	0.495563103745658	1.9535656436200564
7	0.3213608485223657	1.4779808252232378
8	0.23311151391108717	1.225269170274863
9	0.1741832967845564	1.0299752999733893
>10	0.8603177749448706	9.627853922779758
>50	0.0334007942450729	1.4670596963083902
>100	0.014820370006978875	1.7684134081118716
>500	0.001211558189144271	0.5124509319029841
>1k	2.795903513409856E-4	0.334816787626421
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2091	0.12772304805465154	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2054	0.12546300368448315	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.10822802748214E-5
2	0.0	0.0	0.0	0.0	6.10822802748214E-5
3	0.0	0.0	0.0	0.0	6.10822802748214E-5
4	0.0	0.0	0.0	6.10822802748214E-5	6.10822802748214E-5
5	0.0	0.0	0.0	6.10822802748214E-5	6.10822802748214E-5
6	0.0	0.0	0.0	6.10822802748214E-5	1.832468408244642E-4
7	0.0	0.0	0.0	6.10822802748214E-5	1.832468408244642E-4
8	0.0	0.0	0.0	6.10822802748214E-5	1.832468408244642E-4
9	0.0	0.0	0.0	6.10822802748214E-5	1.832468408244642E-4
10-11	0.0	0.0	0.0	6.10822802748214E-5	1.832468408244642E-4
12-13	0.0	0.0	0.0	6.10822802748214E-5	1.832468408244642E-4
14-15	0.0	0.0	0.0	6.10822802748214E-5	1.832468408244642E-4
16-17	0.0	0.0	0.0	6.10822802748214E-5	2.443291210992856E-4
18-19	0.0	0.0	0.0	6.10822802748214E-5	2.443291210992856E-4
20-21	0.0	0.0	0.0	6.10822802748214E-5	2.443291210992856E-4
22-23	0.0	0.0	0.0	2.443291210992856E-4	3.05411401374107E-4
24-25	0.0	0.0	0.0	0.0011605633252216065	4.886582421985712E-4
26-27	0.0	0.0	0.0	0.0025043734912676774	5.497405224733925E-4
28-29	0.0	0.0	0.0	0.006444180568993658	6.108228027482139E-4
30-31	0.0	0.0	0.0	0.018752260044370167	6.108228027482139E-4
32-33	3.05411401374107E-5	0.0	0.0	0.05088153946892622	6.108228027482139E-4
34-35	6.10822802748214E-5	3.05411401374107E-5	0.0	0.09858680036356174	7.329873632978568E-4
36-37	6.10822802748214E-5	9.16234204122321E-5	0.0	0.16140992562621553	7.329873632978568E-4
38-39	6.10822802748214E-5	1.221645605496428E-4	0.0	0.24875758641921014	7.329873632978568E-4
40-41	6.10822802748214E-5	1.527057006870535E-4	0.0	0.3463670702983747	7.329873632978568E-4
42-43	6.10822802748214E-5	1.832468408244642E-4	0.0	0.45576543427057986	7.329873632978568E-4
44-45	6.10822802748214E-5	1.832468408244642E-4	0.0	0.6088681697794197	7.329873632978568E-4
46-47	6.10822802748214E-5	1.832468408244642E-4	0.0	0.7802650482305684	7.329873632978568E-4
48-49	6.10822802748214E-5	1.832468408244642E-4	0.0	0.9403006225506005	7.329873632978568E-4
50-51	6.10822802748214E-5	1.832468408244642E-4	0.0	1.0918457599124323	7.329873632978568E-4
52-53	6.10822802748214E-5	1.832468408244642E-4	0.0	1.2567068343741754	7.329873632978568E-4
54-55	6.10822802748214E-5	1.832468408244642E-4	0.0	1.4592251346253458	7.329873632978568E-4
56-57	6.10822802748214E-5	1.832468408244642E-4	0.0	1.7074024393819451	7.329873632978568E-4
58-59	6.10822802748214E-5	1.832468408244642E-4	0.0	1.9578092473685753	7.635285034352675E-4
60-61	6.10822802748214E-5	1.832468408244642E-4	0.0	2.2011305108433263	7.940696435726781E-4
62-63	6.10822802748214E-5	1.832468408244642E-4	0.0	2.451781647951056	9.16234204122321E-4
64-65	6.10822802748214E-5	1.832468408244642E-4	0.0	2.754200017591697	9.467753442597317E-4
66-67	6.10822802748214E-5	1.832468408244642E-4	0.0	3.1224345442284576	0.001007857624534553
68-69	6.10822802748214E-5	1.832468408244642E-4	0.0	3.518064473568476	0.0010383987646719638
70-71	6.10822802748214E-5	1.832468408244642E-4	0.0	3.899401149324186	0.001191104465359017
72-73	6.10822802748214E-5	1.832468408244642E-4	0.0	4.286326853725042	0.0014659747265957136
74-75	6.10822802748214E-5	1.832468408244642E-4	0.0	4.730639360444092	0.0014659747265957136
76-77	6.10822802748214E-5	1.832468408244642E-4	0.0	5.265262018549467	0.0014965158667331242
78-79	6.10822802748214E-5	1.832468408244642E-4	0.0	5.8585236657186694	0.0015270570068705348
80-81	6.10822802748214E-5	1.832468408244642E-4	0.0	6.4467460247652	0.0015575981470079456
82-83	6.10822802748214E-5	2.1378798096187489E-4	0.0	7.031914269797989	0.0016492215674201777
84-85	6.10822802748214E-5	2.443291210992856E-4	0.0	7.660970133208236	0.0016492215674201777
86-87	6.10822802748214E-5	2.443291210992856E-4	0.0	8.3662872235416	0.0016492215674201777
88-89	6.10822802748214E-5	2.443291210992856E-4	0.0	9.140352420324273	0.001710303847694999
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2505	0.0	62.42162	1
GTATCAA	4360	0.0	41.205215	1
TCAACGC	5930	0.0	30.007719	4
ATCAACG	5995	0.0	29.682365	3
TATCAAC	6115	0.0	29.41028	2
CAACGCA	6100	0.0	29.23216	5
AACGCAG	6410	0.0	27.667534	6
GTGGTAT	960	0.0	25.744173	1
GTACATG	6475	0.0	24.36942	1
ACGCAGA	7325	0.0	23.950346	7
TAAGGTG	800	0.0	23.775494	5
TACATGG	6785	0.0	23.149126	2
CGCAGAG	7530	0.0	22.856337	8
ACATGGG	6780	0.0	22.536297	3
TGGTATC	1120	0.0	21.184021	2
CATGGGG	5040	0.0	21.08987	4
GCAGAGT	7870	0.0	20.600258	9
ATGGGGG	3295	0.0	20.059444	5
CAGAGTA	7595	0.0	19.562092	10-11
GAGTACT	3625	0.0	19.436666	12-13
>>END_MODULE