FastQCFastQC Report
Mon 15 Aug 2016
SRR2921362_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921362_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2645456
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21650.053.4933431
GTATCAA41300.042.2927131
TCAACGC49200.035.3102044
CAACGCA50100.034.5798385
ATCAACG51600.033.391273
AACGCAG54000.032.336926
TATCAAC54000.031.7332172
GTACATG92550.026.9759731
TACATGG93350.026.6451152
ACGCAGA65700.026.2201467
ACATGGG96600.024.9119013
CGCAGAG68600.024.3530588
CATGGGG83700.022.2869854
GAGTACA67350.022.0584261
GCAGAGT75800.020.7249629
AGTACAT69800.019.9936712
ATGGGGG53950.019.7071745
CGTATAC1708.0036916E-519.5445253
AGAGTAC65750.018.1181110-11
TAGGACA9150.017.6377054
Produced by FastQC (version 0.11.5)