##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921362_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2645456 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.607452930610073 31.0 31.0 34.0 27.0 34.0 2 30.666118431000175 33.0 31.0 34.0 27.0 34.0 3 30.7191006011818 33.0 31.0 34.0 27.0 34.0 4 34.174611862756365 37.0 35.0 37.0 30.0 37.0 5 34.16929482100628 37.0 35.0 37.0 30.0 37.0 6 34.21322562159416 37.0 35.0 37.0 30.0 37.0 7 34.13347868949625 37.0 35.0 37.0 30.0 37.0 8 34.10048173169389 37.0 35.0 37.0 30.0 37.0 9 35.68224079326966 39.0 37.0 39.0 30.0 39.0 10-11 35.5808922922929 39.0 37.0 39.0 30.0 39.0 12-13 35.46545586091774 39.0 37.0 39.0 30.0 39.0 14-15 36.6349081217 40.0 37.0 41.0 30.0 41.0 16-17 36.58018447481266 40.0 37.0 41.0 30.0 41.0 18-19 36.52370177390968 40.0 37.0 41.0 29.5 41.0 20-21 36.45646799644371 40.0 37.0 41.0 29.0 41.0 22-23 36.34635106386196 40.0 37.0 41.0 28.5 41.0 24-25 36.24806290484514 40.0 37.0 41.0 28.0 41.0 26-27 36.100774308852614 40.0 36.0 41.0 27.0 41.0 28-29 35.96090484967431 40.0 36.0 41.0 27.0 41.0 30-31 35.81590943111509 40.0 36.0 41.0 27.0 41.0 32-33 35.640565558451925 40.0 36.0 41.0 26.0 41.0 34-35 35.48103408259294 39.5 35.0 41.0 25.5 41.0 36-37 35.31934683472339 39.0 35.0 41.0 25.0 41.0 38-39 35.55678227118501 39.5 35.5 41.0 26.0 41.0 40-41 35.68151898954282 40.0 36.0 41.0 26.0 41.0 42-43 35.525990982273 40.0 35.0 41.0 25.0 41.0 44-45 35.40893290230493 40.0 35.0 41.0 25.0 41.0 46-47 35.254432317150616 40.0 35.0 41.0 24.0 41.0 48-49 35.10957789507745 40.0 35.0 41.0 24.0 41.0 50-51 33.39326017896347 37.0 32.5 39.5 20.5 40.0 52-53 33.5560438351649 37.5 33.5 39.5 21.5 40.0 54-55 34.41161504859654 39.0 34.0 40.5 22.0 41.0 56-57 34.41896066311441 39.0 34.5 41.0 22.0 41.0 58-59 34.23327528410981 38.5 34.0 41.0 22.0 41.0 60-61 33.98172848083657 38.0 34.0 40.5 20.5 41.0 62-63 33.687475240563444 37.0 34.0 40.0 20.0 41.0 64-65 33.39438380377523 37.0 34.0 40.0 19.0 41.0 66-67 33.05273930089935 36.0 33.5 39.5 17.5 41.0 68-69 32.68420718394107 36.0 33.0 39.0 14.0 41.0 70-71 32.30550782171391 35.0 33.0 39.0 12.0 40.5 72-73 31.950808291651796 35.0 33.0 37.5 7.5 40.0 74-75 31.56059031032835 35.0 32.5 37.0 6.5 39.0 76-77 31.197304358870454 35.0 32.0 36.5 2.0 39.0 78-79 30.864841259881093 35.0 32.0 36.0 2.0 38.5 80-81 30.5286358949081 35.0 32.0 36.0 2.0 37.0 82-83 30.24249411065616 35.0 31.0 35.0 2.0 37.0 84-85 30.021424094749637 35.0 31.0 35.0 2.0 36.5 86-87 29.80736912653244 35.0 31.0 35.0 2.0 36.0 88-89 29.614817256457865 35.0 31.0 35.0 2.0 36.0 90-91 29.37322601472109 35.0 31.0 35.0 2.0 35.5 92-93 29.192188038659495 35.0 31.0 35.0 2.0 35.0 94-95 28.995400603903448 34.5 31.0 35.0 2.0 35.0 96-97 28.749529381702057 34.0 30.0 35.0 2.0 35.0 98-99 28.5362340178782 34.0 30.0 35.0 2.0 35.0 100-101 27.501908744654987 33.5 27.5 35.0 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 85385.0 3 14939.0 4 10052.0 5 7812.0 6 7095.0 7 7184.0 8 7461.0 9 7878.0 10 8727.0 11 9227.0 12 9246.0 13 9364.0 14 9805.0 15 10670.0 16 10817.0 17 11334.0 18 10643.0 19 9875.0 20 9977.0 21 10626.0 22 11571.0 23 13367.0 24 15756.0 25 18555.0 26 21995.0 27 26231.0 28 31019.0 29 36996.0 30 44436.0 31 53941.0 32 68475.0 33 89311.0 34 129341.0 35 190104.0 36 320093.0 37 587738.0 38 609628.0 39 108768.0 40 14.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.71841572326212 17.459908064147097 12.779991188377734 25.041685024213052 2 16.236322576716677 19.011235428114553 39.33829466722793 25.414147327940846 3 19.951502719841226 22.935915821065304 28.653671029304096 28.45891042978937 4 13.790176974215182 14.864638967911366 35.49862070999964 35.846563347873804 5 14.79324088069889 36.9208742246084 33.60227567958208 14.683609215110641 6 34.731323821251294 33.833512225201865 16.694864662850893 14.740299290695946 7 30.0295567172914 31.319837101581648 20.00212469622893 18.64848148489802 8 27.516410592611244 34.5921152027004 19.24041319683108 18.651061007857273 9 27.484769877988885 15.547068856068776 18.794044287867738 38.17411697807459 10-11 26.610620216837038 24.808163063144985 26.802272835972918 21.77894388404506 12-13 26.760968377821392 22.02755736927945 27.05484987655843 24.156624376340734 14-15 23.92666944620135 23.62685786839943 24.216970099202918 28.229502586196304 16-17 24.307855477811408 26.879323480979995 24.978400319127015 23.834420722081585 18-19 24.362557016164157 26.280784191550115 26.210686069302618 23.14597272298311 20-21 24.739899604801668 25.36988401163362 26.34770874200491 23.5425076415598 22-23 24.67518592948861 25.114586870840554 25.980190463800763 24.23003673587008 24-25 24.49352887355754 25.28211970917416 25.958775911591303 24.265575505676996 26-27 24.138422594677802 25.64076289386024 25.99287124280219 24.227943268659764 28-29 24.2240040530718 25.968318362740472 25.749501636635685 24.05817594755204 30-31 24.730951490269682 25.45470251976431 25.738206958240088 24.076139031725923 32-33 24.417889446628273 25.539334992185854 25.671120768848652 24.371654792337218 34-35 24.57828811086343 25.720791297294852 25.83102868761744 23.869891904224275 36-37 24.585400667683395 25.630434992159078 25.882833906431713 23.901330433725818 38-39 24.52259572137951 25.683971211239992 25.781371531850166 24.012061535530325 40-41 24.599208538574814 25.464270546214983 25.769673271251037 24.166847643959166 42-43 24.39113718904469 25.517850115763757 25.63662490233084 24.45438779286071 44-45 24.1987106365198 25.648681300102183 25.854500077719262 24.298107985658763 46-47 24.446342469223787 25.528645763317108 25.62195234169596 24.403059425763143 48-49 24.17979671640361 25.67090156803249 25.72804348389982 24.42125823166408 50-51 24.345715925341306 25.670121811412976 25.86753972072191 24.116622542523803 52-53 24.560208335621205 25.484211048498167 25.795506485116476 24.16007413076415 54-55 24.02076977446459 25.35050035775679 26.137300220902276 24.491429646876345 56-57 24.12672762324449 25.543232405363582 25.822742432485196 24.50729753890673 58-59 24.224138665586104 25.547437653788585 25.969936728335156 24.258486952290152 60-61 24.480559049234554 25.51396846713272 25.986135869838968 24.019336613793765 62-63 24.37338906911201 25.428811529701722 26.09645551653176 24.101343884654508 64-65 24.05082130741142 25.25068678977757 26.1576393670097 24.540852535801307 66-67 24.14740453505809 25.417663854344962 25.95385419348975 24.481077417107198 68-69 24.13218433677409 25.38169348117254 26.059026877113233 24.42709530494014 70-71 24.10241838218075 25.21823217127735 26.07429330941461 24.60505613712729 72-73 24.01263084318016 25.564697100013987 25.87221371638081 24.550458340425042 74-75 23.980545983202557 25.59241831777923 25.989162426159513 24.437873272858695 76-77 24.021703530231406 25.178234373136394 25.99813710103387 24.801924995598334 78-79 23.92692929465462 25.221997487882053 26.096201657210088 24.754871560253243 80-81 24.15179496347426 25.45730558334182 25.81025326557566 24.580646187608256 82-83 24.1388935706048 25.433540570277184 25.94230947678195 24.485256382336065 84-85 24.13640175033688 25.54978287430039 25.816219172354554 24.49759620300817 86-87 23.915555037030753 25.744440841707174 25.762679699147867 24.57732442211421 88-89 23.80207979209276 25.437784928808398 25.960886333019385 24.79924894607946 90-91 24.030119963955133 25.209343181152732 26.036545616870633 24.723991238021505 92-93 24.132026352996437 25.130633322316616 26.05234424051418 24.684996084172766 94-95 24.074719506355432 25.109560726646745 26.0446531940816 24.771066572916226 96-97 23.857928981159624 24.982956849717393 26.16640214030524 24.992712028817746 98-99 23.668775892213013 24.95378157706483 26.136681527650275 25.24076100307188 100-101 23.939351979798644 24.953662916827753 26.19344152927812 24.91354357409548 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 31.0 1 28.0 2 49.0 3 79.5 4 78.5 5 94.5 6 161.5 7 304.0 8 463.0 9 538.0 10 476.5 11 398.0 12 431.0 13 486.0 14 543.0 15 629.5 16 721.5 17 797.5 18 882.0 19 958.0 20 1039.0 21 1144.0 22 1249.5 23 1444.5 24 1655.5 25 1960.0 26 2603.5 27 3598.0 28 5058.5 29 7540.5 30 11062.0 31 15326.0 32 21103.0 33 25919.5 34 29672.0 35 34752.0 36 41526.5 37 48495.0 38 54598.5 39 60852.5 40 66467.0 41 71514.0 42 76523.5 43 82462.5 44 88823.5 45 95729.0 46 101748.5 47 107051.5 48 112055.0 49 114776.5 50 115732.5 51 116696.5 52 117285.5 53 116651.5 54 114844.0 55 109608.5 56 102518.5 57 93877.5 58 84545.5 59 74286.0 60 62289.0 61 51953.5 62 43124.5 63 33957.0 64 25254.0 65 19021.0 66 14677.5 67 11560.0 68 9259.5 69 7343.5 70 5840.5 71 4480.0 72 3368.0 73 2654.0 74 2186.5 75 1685.0 76 1295.5 77 985.0 78 685.5 79 458.5 80 308.5 81 229.5 82 173.0 83 117.0 84 92.5 85 77.5 86 69.0 87 72.5 88 61.0 89 50.0 90 40.5 91 31.0 92 24.0 93 17.0 94 18.0 95 19.0 96 15.0 97 11.5 98 8.0 99 5.5 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04615461379815049 2 0.05326113910040462 3 0.044755989137600476 4 0.05072849444481406 5 0.04335736447705046 6 0.01772851258913397 7 0.01406184793850285 8 0.019580745247700208 9 0.020147755245220483 10-11 0.02141407757301577 12-13 0.02579895488717257 14-15 0.042658052146775455 16-17 0.02801029387750165 18-19 0.022377994568800238 20-21 0.02676287188295704 22-23 0.04437798247258696 24-25 0.03989860349217677 26-27 0.030826443531852352 28-29 0.020733665575991437 30-31 0.013135731609219733 32-33 0.009601369291343346 34-35 0.009658070291095373 36-37 0.0015309269933047461 38-39 0.007087624969003454 40-41 0.003647764317380444 42-43 0.1952404424794818 44-45 0.17163392624938764 46-47 0.17762533188985188 48-49 0.17624560756255256 50-51 0.1743177735709836 52-53 0.1783057438868762 54-55 0.17749302955709714 56-57 0.18682979418293105 58-59 0.18365453819681749 60-61 0.18798271451122225 62-63 0.1852988671896263 64-65 0.1856012725216371 66-67 0.17751192989034784 68-69 0.1761133052297978 70-71 0.16214595895754835 72-73 0.16860987292927948 74-75 0.16855317192952746 76-77 0.16673873993746258 78-79 0.19106346883108244 80-81 0.19894490779661425 82-83 0.1950892398134764 84-85 0.19089336583182634 86-87 0.20745005775941844 88-89 0.2053521207685934 90-91 0.20461500777181704 92-93 0.20898098475272317 94-95 0.2201510817038726 96-97 0.2204723873691341 98-99 0.20962359608324616 100-101 0.22043458670263275 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2645456.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.824046662506355 #Duplication Level Percentage of deduplicated Percentage of total 1 77.58396902682719 45.638030142965306 2 12.140710373962298 14.283314271078664 3 4.1205055081776125 7.2715442485846316 4 1.928241059731335 4.53707768296787 5 1.0768630145056979 3.167272010720522 6 0.6988088769567881 2.466405959176786 7 0.46657284965354795 1.9211992155635207 8 0.33798725601274177 1.59054224952208 9 0.2537633405880588 1.3434647929186916 >10 1.322224232133223 13.543442246104975 >50 0.054070115101030644 2.1267421248173117 >100 0.014997479309909214 1.500076329930807 >500 9.651502804838917E-4 0.39783465894321723 >1k 3.217167601519238E-4 0.21305406670567056 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 3.780066650135175E-5 10-11 0.0 0.0 0.0 3.780066650135175E-5 3.780066650135175E-5 12-13 0.0 0.0 0.0 7.56013330027035E-5 3.780066650135175E-5 14-15 0.0 0.0 0.0 7.56013330027035E-5 5.670099975202763E-5 16-17 0.0 0.0 0.0 7.56013330027035E-5 7.56013330027035E-5 18-19 0.0 0.0 0.0 7.56013330027035E-5 7.56013330027035E-5 20-21 0.0 0.0 0.0 7.56013330027035E-5 9.450166625337938E-5 22-23 0.0 0.0 0.0 7.56013330027035E-5 1.3230233275473113E-4 24-25 0.0 0.0 0.0 1.7010299925608287E-4 1.51202666005407E-4 26-27 0.0 0.0 0.0 3.02405332010814E-4 1.7010299925608287E-4 28-29 0.0 0.0 0.0 0.0016443289928088013 1.8900333250675876E-4 30-31 0.0 0.0 0.0 0.007295528634760888 1.8900333250675876E-4 32-33 0.0 0.0 0.0 0.018862532584174523 1.8900333250675876E-4 34-35 0.0 0.0 0.0 0.034530908848984826 1.8900333250675876E-4 36-37 0.0 1.8900333250675877E-5 0.0 0.06153948506420065 1.8900333250675876E-4 38-39 0.0 3.780066650135175E-5 0.0 0.1050669525405072 2.268039990081105E-4 40-41 0.0 3.780066650135175E-5 0.0 0.1574775766446314 2.268039990081105E-4 42-43 0.0 3.780066650135175E-5 0.0 0.21058751307903062 2.4570433225878637E-4 44-45 0.0 3.780066650135175E-5 0.0 0.276984383788655 2.6460466550946225E-4 46-47 0.0 3.780066650135175E-5 0.0 0.3534929327873909 2.8350499876013814E-4 48-49 0.0 5.670099975202763E-5 0.0 0.4416440870685432 3.4020599851216575E-4 50-51 0.0 7.56013330027035E-5 0.0 0.5349361319938793 3.4020599851216575E-4 52-53 0.0 7.56013330027035E-5 0.0 0.6336336722289087 3.969069982641934E-4 54-55 0.0 7.56013330027035E-5 0.0 0.751874157045137 4.158073315148693E-4 56-57 0.0 7.56013330027035E-5 0.0 0.8848380014636418 4.158073315148693E-4 58-59 0.0 7.56013330027035E-5 0.0 1.0356059598042833 4.158073315148693E-4 60-61 0.0 7.56013330027035E-5 0.0 1.1962020914352762 4.158073315148693E-4 62-63 0.0 7.56013330027035E-5 0.0 1.368535330014939 4.158073315148693E-4 64-65 0.0 1.1340199950405525E-4 0.0 1.550866844884209 4.347076647655451E-4 66-67 0.0 1.1340199950405525E-4 0.0 1.7721330462498714 4.53607998016221E-4 68-69 0.0 1.1340199950405525E-4 0.0 2.013622604193757 4.53607998016221E-4 70-71 0.0 1.1340199950405525E-4 0.0 2.275845827713634 4.53607998016221E-4 72-73 1.8900333250675877E-5 1.1340199950405525E-4 0.0 2.5485587361876365 5.103089977682486E-4 74-75 5.670099975202763E-5 1.1340199950405525E-4 0.0 2.8377149345897266 5.292093310189245E-4 76-77 7.56013330027035E-5 1.1340199950405525E-4 0.0 3.168036058811789 5.292093310189245E-4 78-79 7.56013330027035E-5 1.1340199950405525E-4 0.0 3.5374052715297477 5.292093310189245E-4 80-81 7.56013330027035E-5 1.1340199950405525E-4 0.0 3.9257504188313845 5.292093310189245E-4 82-83 7.56013330027035E-5 1.1340199950405525E-4 0.0 4.3304821550613575 6.23710997272304E-4 84-85 7.56013330027035E-5 1.1340199950405525E-4 0.0 4.752337593216444 6.426113305229798E-4 86-87 7.56013330027035E-5 1.1340199950405525E-4 0.0 5.20526517923564 6.615116637736557E-4 88-89 7.56013330027035E-5 1.1340199950405525E-4 0.0 5.70582160504654 6.804119970243315E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2165 0.0 53.493343 1 GTATCAA 4130 0.0 42.292713 1 TCAACGC 4920 0.0 35.310204 4 CAACGCA 5010 0.0 34.579838 5 ATCAACG 5160 0.0 33.39127 3 AACGCAG 5400 0.0 32.33692 6 TATCAAC 5400 0.0 31.733217 2 GTACATG 9255 0.0 26.975973 1 TACATGG 9335 0.0 26.645115 2 ACGCAGA 6570 0.0 26.220146 7 ACATGGG 9660 0.0 24.911901 3 CGCAGAG 6860 0.0 24.353058 8 CATGGGG 8370 0.0 22.286985 4 GAGTACA 6735 0.0 22.058426 1 GCAGAGT 7580 0.0 20.724962 9 AGTACAT 6980 0.0 19.993671 2 ATGGGGG 5395 0.0 19.707174 5 CGTATAC 170 8.0036916E-5 19.544525 3 AGAGTAC 6575 0.0 18.11811 10-11 TAGGACA 915 0.0 17.637705 4 >>END_MODULE