FastQCFastQC Report
Mon 15 Aug 2016
SRR2921362_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921362_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2645456
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19400.053.636381
GTATCAA37450.046.0544781
TCAACGC49050.033.864524
CAACGCA49700.033.377255
ATCAACG51650.032.251713
TATCAAC53700.031.1972522
AACGCAG53950.031.0104486
GTACATG90200.026.3905371
TACATGG90350.025.7385372
ACATGGG95200.023.8789083
ACGCAGA70400.023.3588837
CGCAGAG72050.022.757988
CATGGGG82100.021.7928524
ATGGGGG52350.020.9737835
CGTATAC2351.1437078E-720.1951963
GCAGAGT81750.018.953029
AGAGTAC68450.018.262655
GAGTACA69450.017.8560471
CAGAGTA76500.017.3705064
AGTACAT69850.017.1897682
Produced by FastQC (version 0.11.5)