##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921362_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2645456
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.039459359747433	33.0	31.0	34.0	28.0	34.0
2	31.180473234104063	33.0	31.0	34.0	28.0	34.0
3	31.25336047925197	34.0	31.0	34.0	28.0	34.0
4	34.745795431865055	37.0	35.0	37.0	32.0	37.0
5	34.451695284291254	37.0	35.0	37.0	31.0	37.0
6	34.403568609721724	37.0	35.0	37.0	31.0	37.0
7	34.25133663156749	37.0	35.0	37.0	30.0	37.0
8	34.20573504152025	37.0	35.0	37.0	30.0	37.0
9	35.786185822028415	39.0	37.0	39.0	30.0	39.0
10-11	35.61656761632021	39.0	37.0	39.0	30.0	39.0
12-13	35.534838984280974	39.0	37.0	39.0	30.0	39.0
14-15	36.723050203821195	40.0	37.0	41.0	30.0	41.0
16-17	36.690413675373925	40.0	37.0	41.0	30.0	41.0
18-19	36.63863545642037	40.0	37.0	41.0	30.0	41.0
20-21	36.59057058594057	40.0	37.0	41.0	30.0	41.0
22-23	36.47845588813422	40.0	37.0	41.0	29.0	41.0
24-25	36.40000287285065	40.0	37.0	41.0	29.0	41.0
26-27	36.25054678664094	40.0	37.0	41.0	28.5	41.0
28-29	36.08950007106525	40.0	36.0	41.0	27.0	41.0
30-31	35.944080151021225	40.0	36.0	41.0	27.0	41.0
32-33	35.89636436969657	40.0	36.0	41.0	27.0	41.0
34-35	36.03306178594541	40.0	36.0	41.0	28.0	41.0
36-37	36.01413366920485	40.0	36.0	41.0	27.5	41.0
38-39	35.92168476814584	40.0	36.0	41.0	27.0	41.0
40-41	35.7927727771696	40.0	36.0	41.0	27.0	41.0
42-43	35.68342546615782	40.0	35.5	41.0	26.0	41.0
44-45	35.54101429016396	40.0	35.0	41.0	26.0	41.0
46-47	35.380873278557644	40.0	35.0	41.0	25.5	41.0
48-49	35.217162939016944	39.5	35.0	41.0	24.5	41.0
50-51	35.052500400687066	39.0	35.0	41.0	24.0	41.0
52-53	34.873022836138645	39.0	35.0	41.0	24.0	41.0
54-55	34.652620569005876	39.0	35.0	41.0	23.0	41.0
56-57	34.38780270773734	38.5	34.0	40.5	22.5	41.0
58-59	34.155117114024954	38.0	34.0	40.0	22.0	41.0
60-61	33.87042422932001	37.5	34.0	40.0	21.5	41.0
62-63	33.567287076405734	37.0	34.0	40.0	20.5	41.0
64-65	33.25535049533993	36.5	33.5	39.5	20.0	41.0
66-67	32.919217707646624	36.0	33.0	39.0	18.5	41.0
68-69	32.56177781826649	35.5	33.0	39.0	17.5	40.5
70-71	32.1879197764015	35.0	33.0	38.0	14.0	40.0
72-73	31.804274385965975	35.0	32.0	37.0	11.5	39.5
74-75	31.40764635662056	35.0	32.0	37.0	7.5	39.0
76-77	28.718054278733042	32.0	27.5	34.5	4.5	36.5
78-79	30.35967447578036	34.5	31.0	35.5	3.5	37.0
80-81	30.573655354691212	35.0	31.5	35.0	2.0	37.0
82-83	30.427513441917007	35.0	32.0	35.0	2.0	37.0
84-85	30.21124751271614	35.0	31.5	35.0	2.0	36.0
86-87	29.975654669743136	35.0	31.0	35.0	2.0	36.0
88-89	29.70704842567784	34.0	31.0	35.0	2.0	35.5
90-91	29.525391841708952	34.0	31.0	35.0	2.0	35.0
92-93	29.376370463164008	34.0	31.0	35.0	2.0	35.0
94-95	29.280343351013965	34.0	31.0	35.0	2.0	35.0
96-97	29.090289915991796	34.0	31.0	35.0	2.0	35.0
98-99	28.82446126490102	34.0	30.5	35.0	2.0	35.0
100-101	27.72691191991097	33.0	27.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	68823.0
3	21875.0
4	11653.0
5	8032.0
6	7013.0
7	6701.0
8	6913.0
9	7394.0
10	8116.0
11	8584.0
12	8967.0
13	9468.0
14	10285.0
15	10981.0
16	11629.0
17	12465.0
18	11549.0
19	10308.0
20	9787.0
21	9863.0
22	10463.0
23	11806.0
24	13906.0
25	16744.0
26	20256.0
27	24403.0
28	29073.0
29	35060.0
30	42878.0
31	53033.0
32	67963.0
33	90154.0
34	132513.0
35	196610.0
36	342834.0
37	637031.0
38	594641.0
39	65682.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.38743099569092	19.484121817020103	13.567201036843683	26.56124615044529
2	14.964040035653777	20.815727579739036	37.06722732911929	27.153005055487895
3	18.103756235032613	24.389236145894564	28.542849960401988	28.964157658670832
4	13.39210582223395	16.418292984423186	34.100278905336246	36.08932228800661
5	14.455281202205834	37.363548541385846	33.58258926461497	14.598580991793344
6	34.79112217256831	34.566923538097136	16.32200836010285	14.319945929231706
7	29.746916786305867	31.593102733973073	19.95147146765735	18.708509012063715
8	27.476705958488996	35.2008509805063	18.774039197571348	18.548403863433354
9	27.151689538589945	15.419080869233886	18.632402126514293	38.79682746566188
10-11	26.517256565849184	25.141071073184428	26.72278105814845	21.618891302817943
12-13	26.607527445682734	22.24695019094087	27.03397622901942	24.111546134356978
14-15	23.758947622891778	23.882939925540352	24.141736054787966	28.216376396779907
16-17	24.24393856175105	27.141929945457356	24.837466399894463	23.776665092897133
18-19	24.276441489433083	26.523413128846347	26.125994845110444	23.07415053661013
20-21	24.668296392954804	25.544957840566045	26.273590647130113	23.513155119349037
22-23	24.53296742239749	25.273388957087683	25.982076790265555	24.211566830249268
24-25	24.361132190659077	25.51592810545843	25.90395979439802	24.218979909484474
26-27	24.047964303103065	25.84551051945578	25.94887925533684	24.157645922104315
28-29	24.12351698567415	26.1928946867033	25.671155654885215	24.012432672737337
30-31	24.595557837374763	25.70647655651302	25.691694299908924	24.006271306203292
32-33	24.32345682372903	25.705975356361886	25.61936119504103	24.351206624868055
34-35	24.485599974897635	25.893865382514313	25.83228141566758	23.78825322692047
36-37	24.451537155080878	25.835126041462303	25.92031039082738	23.793026412629434
38-39	24.508821657718283	25.835552710418398	25.742921949559044	23.912703682304272
40-41	24.576965248634334	25.680385420216567	25.71419984837376	24.028449482775336
42-43	24.327507509732598	25.695231499368298	25.560347402157994	24.41691358874111
44-45	24.18218250875681	25.742842815161577	25.75873937449213	24.316235301589487
46-47	24.371082170203863	25.704214636444107	25.575131696165716	24.349571497186318
48-49	24.104741387376528	25.867878245546983	25.67756720949166	24.349813157584833
50-51	24.287033335506997	25.811320255433923	25.733881096774375	24.1677653122847
52-53	24.557213858883014	25.65579676178495	25.64209377154677	24.144895607785262
54-55	23.93623938072734	25.56191327975154	26.01917422751572	24.4826731120054
56-57	24.126346397893016	25.686793818014525	25.674640182451963	24.512219601640496
58-59	24.186833891825845	25.698033733351078	25.852520172265702	24.26261220255737
60-61	24.40865286017119	25.751813862759793	25.80957772462175	24.029955552447262
62-63	24.322595402242356	25.671733488091615	25.864248757554392	24.14142235211164
64-65	23.95973693638848	25.456783284476053	25.9856141223225	24.597865656812967
66-67	24.047210523579267	25.68897207923881	25.759740597363432	24.504076799818495
68-69	24.025648340508813	25.625049271742377	25.812248808639993	24.537053579108818
70-71	24.01033203410834	25.45251337072308	25.85008983144361	24.687064763724962
72-73	23.924352777881754	25.78243067631124	25.619923298635094	24.673293247171905
74-75	23.829741218781912	25.81136870888427	25.64808733747955	24.71080273485427
76-77	23.814866627328446	25.371883527293498	25.823597439290168	24.989652406087888
78-79	23.709114814021905	25.42800039034634	25.968471211579065	24.89441358405269
80-81	23.959917256679592	25.70827552338138	25.58588893189901	24.74591828804002
82-83	23.86996838474876	25.63440338217402	25.73516234480662	24.7604658882706
84-85	23.790539240097573	25.68710015658151	25.635694080341704	24.886666522979215
86-87	23.615198621312942	25.91626378984972	25.580028179782392	24.888509409054947
88-89	23.448301606066742	25.628170697576678	25.742715152668143	25.18081254368843
90-91	23.608694992757965	25.441915840720714	25.85825705814103	25.091132108380286
92-93	23.675862070269606	25.374995059945316	25.847746474691885	25.10139639509319
94-95	23.587318604450722	25.361902738568133	25.804306013176458	25.24647264380469
96-97	23.282618927455527	25.300420950361126	25.928349598613337	25.488610523570003
98-99	23.085797599217823	25.269280470335726	25.864779235281077	25.78014269516538
100-101	23.127163640184982	25.32011857447534	25.917999596284304	25.634718189055373
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	39.0
1	32.0
2	41.0
3	57.0
4	55.0
5	62.5
6	109.5
7	257.0
8	376.5
9	407.0
10	366.0
11	297.5
12	302.5
13	325.0
14	358.0
15	412.0
16	462.0
17	507.5
18	580.0
19	635.5
20	703.5
21	843.0
22	1022.0
23	1245.0
24	1509.0
25	1916.0
26	2638.5
27	3871.5
28	5647.0
29	8098.5
30	11614.0
31	16185.5
32	22115.0
33	26700.5
34	30399.5
35	35102.5
36	41488.0
37	48557.0
38	54804.0
39	60944.5
40	66039.0
41	71205.0
42	76813.5
43	83005.0
44	89668.0
45	96373.0
46	102316.5
47	107694.5
48	112615.0
49	116110.5
50	117513.0
51	118016.0
52	118138.5
53	117046.5
54	115104.0
55	109932.0
56	102265.5
57	93234.5
58	83738.0
59	73379.5
60	61815.5
61	51849.0
62	42685.5
63	33224.5
64	24421.5
65	17970.5
66	13963.0
67	11127.5
68	8778.0
69	6930.0
70	5429.0
71	4096.5
72	3030.0
73	2391.5
74	1940.0
75	1519.0
76	1115.5
77	695.5
78	412.0
79	265.0
80	186.5
81	121.0
82	71.0
83	45.5
84	28.5
85	15.5
86	12.0
87	14.0
88	10.5
89	2.5
90	2.5
91	3.0
92	3.0
93	2.5
94	1.5
95	0.5
96	0.5
97	0.5
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1357799940728555
2	5.292093310189245E-4
3	0.004573880646663562
4	0.004309275981154099
5	0.02460823389237999
6	0.0012852226610459597
7	7.56013330027035E-5
8	0.0013986246605500149
9	0.0
10-11	0.09306524092632801
12-13	1.3230233275473113E-4
14-15	0.004932986978426404
16-17	1.1340199950405527E-4
18-19	2.4570433225878637E-4
20-21	8.316146630297385E-4
22-23	1.3230233275473113E-4
24-25	0.0020223356578223184
26-27	0.002721647988097326
28-29	0.006350511972227094
30-31	0.014855661935031237
32-33	0.014817861268529887
34-35	0.010848791285887954
36-37	6.426113305229798E-4
38-39	2.4570433225878637E-4
40-41	0.005121990310933163
42-43	0.008391747963300089
44-45	0.008675252962060226
46-47	0.009733671624098075
48-49	0.011812708281672423
50-51	0.005802402307957494
52-53	0.0018144319920648843
54-55	0.0011340199950405526
56-57	0.005934704640712225
58-59	0.00907215996032442
60-61	0.007692435633025081
62-63	0.017804113922136676
64-65	0.03207386552639696
66-67	0.034719912181491584
68-69	0.025874556220175277
70-71	0.040030905824931504
72-73	0.024683835225382696
74-75	0.024003423228358363
76-77	0.22504626801579763
78-79	0.062371099727230386
80-81	0.05199481677260933
82-83	0.020034353245716428
84-85	0.031261151196617896
86-87	0.07830408065755015
88-89	0.25487099388536416
90-91	0.30527818266491674
92-93	0.04742093612594577
94-95	0.004989687978178431
96-97	0.009487967291839289
98-99	0.0010017176622858215
100-101	0.0011340199950405526
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2645456.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.15516327087699
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.58457421719189	45.89528155119459
2	12.243898719851531	14.48579655689798
3	4.121370513271195	7.314010368370069
4	1.8918431917296672	4.4764917155866195
5	1.0783057682618744	3.1893676888729816
6	0.6692330928982714	2.375315572600274
7	0.45799918888582547	1.8965111757529165
8	0.3177276114718558	1.5036182985826718
9	0.25141208659937403	1.338509072795205
>10	1.3183084301676868	13.55452510748708
>50	0.051089461095573795	2.0472545209460313
>100	0.013082230100653033	1.3757340168829497
>500	9.62907062273878E-4	0.41699194116702365
>1k	1.925814124516754E-4	0.13059241286371648
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.780066650135175E-5
2	0.0	0.0	0.0	0.0	3.780066650135175E-5
3	0.0	0.0	0.0	0.0	7.56013330027035E-5
4	0.0	0.0	0.0	0.0	7.56013330027035E-5
5	0.0	0.0	0.0	0.0	7.56013330027035E-5
6	0.0	0.0	0.0	0.0	7.56013330027035E-5
7	0.0	0.0	0.0	0.0	7.56013330027035E-5
8	0.0	0.0	0.0	0.0	7.56013330027035E-5
9	0.0	0.0	0.0	0.0	7.56013330027035E-5
10-11	0.0	0.0	0.0	0.0	7.56013330027035E-5
12-13	0.0	0.0	0.0	3.780066650135175E-5	7.56013330027035E-5
14-15	0.0	0.0	0.0	3.780066650135175E-5	7.56013330027035E-5
16-17	0.0	0.0	0.0	3.780066650135175E-5	7.56013330027035E-5
18-19	0.0	0.0	0.0	3.780066650135175E-5	7.56013330027035E-5
20-21	0.0	0.0	0.0	3.780066650135175E-5	1.1340199950405525E-4
22-23	0.0	0.0	0.0	3.780066650135175E-5	1.1340199950405525E-4
24-25	0.0	0.0	0.0	1.3230233275473113E-4	1.1340199950405525E-4
26-27	0.0	0.0	0.0	2.6460466550946225E-4	1.51202666005407E-4
28-29	0.0	0.0	0.0	0.001682129659310153	1.51202666005407E-4
30-31	0.0	0.0	0.0	0.0076546349665237295	1.51202666005407E-4
32-33	0.0	0.0	0.0	0.019505143914697504	1.51202666005407E-4
34-35	0.0	0.0	0.0	0.03538142384526524	1.51202666005407E-4
36-37	0.0	0.0	0.0	0.06187969106271282	1.51202666005407E-4
38-39	0.0	3.780066650135175E-5	0.0	0.10489684954125111	1.8900333250675876E-4
40-41	0.0	3.780066650135175E-5	0.0	0.15604115131758003	1.8900333250675876E-4
42-43	0.0	3.780066650135175E-5	0.0	0.2089431840862218	1.8900333250675876E-4
44-45	0.0	3.780066650135175E-5	0.0	0.2745462407993178	1.8900333250675876E-4
46-47	0.0	3.780066650135175E-5	0.0	0.34978846747025844	1.8900333250675876E-4
48-49	0.0	3.780066650135175E-5	0.0	0.43650319642435936	1.8900333250675876E-4
50-51	0.0	3.780066650135175E-5	0.0	0.5281698126901373	1.8900333250675876E-4
52-53	0.0	3.780066650135175E-5	0.0	0.6245993129350856	1.8900333250675876E-4
54-55	0.0	3.780066650135175E-5	0.0	0.7400614487634647	1.8900333250675876E-4
56-57	0.0	3.780066650135175E-5	0.0	0.8706060505258829	1.8900333250675876E-4
58-59	0.0	3.780066650135175E-5	0.0	1.0189925668769393	1.8900333250675876E-4
60-61	0.0	3.780066650135175E-5	0.0	1.1767158478538293	1.8900333250675876E-4
62-63	0.0	3.780066650135175E-5	0.0	1.3460628337798852	1.8900333250675876E-4
64-65	0.0	3.780066650135175E-5	0.0	1.5252757936627939	1.8900333250675876E-4
66-67	0.0	3.780066650135175E-5	0.0	1.7415145063837767	1.8900333250675876E-4
68-69	0.0	3.780066650135175E-5	0.0	1.9776930706842224	1.8900333250675876E-4
70-71	0.0	3.780066650135175E-5	0.0	2.2350022075589235	1.8900333250675876E-4
72-73	0.0	3.780066650135175E-5	0.0	2.5027821290544994	2.268039990081105E-4
74-75	0.0	3.780066650135175E-5	0.0	2.789311181134746	2.4570433225878637E-4
76-77	0.0	3.780066650135175E-5	0.0	3.116475949703945	2.8350499876013814E-4
78-79	0.0	3.780066650135175E-5	0.0	3.4838795277638335	3.02405332010814E-4
80-81	0.0	3.780066650135175E-5	0.0	3.8698054324093842	3.02405332010814E-4
82-83	0.0	3.780066650135175E-5	0.0	4.2714942149859985	3.4020599851216575E-4
84-85	0.0	3.780066650135175E-5	0.0	4.689607387157451	3.4020599851216575E-4
86-87	0.0	3.780066650135175E-5	0.0	5.139340816857283	3.5910633176284163E-4
88-89	0.0	3.780066650135175E-5	0.0	5.637610302344851	3.780066650135175E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1940	0.0	53.63638	1
GTATCAA	3745	0.0	46.054478	1
TCAACGC	4905	0.0	33.86452	4
CAACGCA	4970	0.0	33.37725	5
ATCAACG	5165	0.0	32.25171	3
TATCAAC	5370	0.0	31.197252	2
AACGCAG	5395	0.0	31.010448	6
GTACATG	9020	0.0	26.390537	1
TACATGG	9035	0.0	25.738537	2
ACATGGG	9520	0.0	23.878908	3
ACGCAGA	7040	0.0	23.358883	7
CGCAGAG	7205	0.0	22.75798	8
CATGGGG	8210	0.0	21.792852	4
ATGGGGG	5235	0.0	20.973783	5
CGTATAC	235	1.1437078E-7	20.195196	3
GCAGAGT	8175	0.0	18.95302	9
AGAGTAC	6845	0.0	18.26265	5
GAGTACA	6945	0.0	17.856047	1
CAGAGTA	7650	0.0	17.370506	4
AGTACAT	6985	0.0	17.189768	2
>>END_MODULE