FastQCFastQC Report
Mon 15 Aug 2016
SRR2921361_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921361_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2922663
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23400.044.415681
GTATCAA41200.038.5883031
TCAACGC48950.032.0965044
CAACGCA50100.031.5470815
ATCAACG50300.031.0442093
TATCAAC51150.030.9000172
AACGCAG54700.029.0572176
GTACATG86150.028.0946061
TACATGG88900.025.8406662
ACATGGG93700.024.0099623
ACGCAGA64700.023.984017
CGCAGAG68350.022.6368968
CATGGGG80700.022.1154984
GAGTACA64350.018.5111431
GCAGAGT77800.018.4835749
ATGGGGG52700.018.462735
CTATACG1901.8771757E-417.4874654
AGAGTAC63050.017.46358110-11
AGTACAT66050.016.7433832
TATACTG11250.016.4537435
Produced by FastQC (version 0.11.5)