##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921361_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2922663
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.172659660042914	31.0	31.0	34.0	26.0	34.0
2	30.241330936888723	31.0	31.0	34.0	26.0	34.0
3	30.28379974016847	33.0	31.0	34.0	26.0	34.0
4	33.728575959664184	37.0	35.0	37.0	28.0	37.0
5	33.71657389168713	37.0	35.0	37.0	28.0	37.0
6	33.75233682432768	37.0	35.0	37.0	29.0	37.0
7	33.666505512267406	37.0	35.0	37.0	28.0	37.0
8	33.62942357705969	37.0	35.0	37.0	28.0	37.0
9	35.16658643162075	39.0	35.0	39.0	28.0	39.0
10-11	35.05681667711946	39.0	35.0	39.0	28.0	39.0
12-13	34.93929885176635	39.0	35.0	39.0	27.0	39.0
14-15	36.05425120857245	40.0	36.0	41.0	27.0	41.0
16-17	36.00205018505383	40.0	36.0	41.0	27.0	41.0
18-19	35.94698738787196	40.0	36.0	41.0	27.0	41.0
20-21	35.88548388917915	40.0	36.0	41.0	27.0	41.0
22-23	35.76787436663071	40.0	36.0	41.0	27.0	41.0
24-25	35.66503151406782	40.0	36.0	41.0	26.0	41.0
26-27	35.51653663114769	40.0	36.0	41.0	25.0	41.0
28-29	35.37340654738504	40.0	36.0	41.0	25.0	41.0
30-31	35.23531193983022	39.5	35.0	41.0	24.0	41.0
32-33	35.06690114460682	39.0	35.0	41.0	24.0	41.0
34-35	34.908383381867836	39.0	35.0	41.0	23.5	41.0
36-37	34.74924443906123	39.0	35.0	41.0	23.0	41.0
38-39	34.99187299391001	39.0	35.0	41.0	24.0	41.0
40-41	35.12005147360472	40.0	35.0	41.0	23.5	41.0
42-43	34.97370531600804	40.0	35.0	41.0	22.5	41.0
44-45	34.86231016028875	40.0	35.0	41.0	22.0	41.0
46-47	34.71838439806437	40.0	35.0	41.0	20.5	41.0
48-49	34.5816510490604	39.0	35.0	41.0	19.0	41.0
50-51	32.8855134854754	37.0	32.5	39.5	15.5	40.0
52-53	33.04201938437651	37.5	32.5	39.5	14.5	40.0
54-55	33.90217551595924	39.0	34.0	40.5	14.5	41.0
56-57	33.91780150499733	39.0	34.0	41.0	13.5	41.0
58-59	33.74233618450023	38.0	34.0	41.0	13.0	41.0
60-61	33.502749547245095	38.0	34.0	40.0	9.5	41.0
62-63	33.21727462249325	37.0	33.0	40.0	8.0	41.0
64-65	32.92882621089055	37.0	33.0	40.0	7.0	41.0
66-67	32.58909015510854	36.0	33.0	39.5	2.0	41.0
68-69	32.22739091027601	36.0	32.5	39.0	2.0	41.0
70-71	31.846802898589402	35.0	32.0	39.0	2.0	40.5
72-73	31.49117602679474	35.0	32.0	37.5	2.0	40.0
74-75	31.09625639356984	35.0	32.0	37.0	2.0	39.0
76-77	30.725526685765686	35.0	31.0	36.5	2.0	39.0
78-79	30.386421732509014	35.0	31.0	36.0	2.0	38.5
80-81	30.046364052235923	35.0	31.0	36.0	2.0	37.0
82-83	29.760234929583056	35.0	31.0	35.0	2.0	37.0
84-85	29.529062878614468	35.0	31.0	35.0	2.0	36.5
86-87	29.309590089586106	35.0	30.5	35.0	2.0	36.0
88-89	29.118337625651677	35.0	30.0	35.0	2.0	36.0
90-91	28.876472928969232	34.0	30.0	35.0	2.0	35.5
92-93	28.693387332032465	34.0	30.0	35.0	2.0	35.0
94-95	28.496585990242462	34.0	30.0	35.0	2.0	35.0
96-97	28.25605346904518	34.0	29.5	35.0	2.0	35.0
98-99	28.033599152553684	34.0	29.0	35.0	2.0	35.0
100-101	27.00182864052407	33.0	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	117838.0
3	19260.0
4	12952.0
5	9532.0
6	8757.0
7	8778.0
8	9125.0
9	9656.0
10	10285.0
11	11117.0
12	10912.0
13	11092.0
14	11632.0
15	12226.0
16	12363.0
17	12684.0
18	12212.0
19	11638.0
20	11858.0
21	12328.0
22	13435.0
23	15585.0
24	18287.0
25	21524.0
26	26081.0
27	31269.0
28	36492.0
29	43095.0
30	51995.0
31	62879.0
32	78607.0
33	102917.0
34	143387.0
35	207532.0
36	343614.0
37	622398.0
38	654783.0
39	112529.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.80335511494825	18.674200373741428	13.775408033010288	24.74703647830003
2	15.886789520297073	19.558362321326296	39.2815291281829	25.27331903019373
3	19.096476977015012	23.824820336713337	29.1788444725598	27.89985821371185
4	13.705173472678386	15.890279463789936	36.06737749623942	34.33716956729225
5	14.839532332296695	36.847059790059575	33.818824121411495	14.494583756232233
6	33.64832507640666	34.66324777316214	17.26867458788656	14.419752562544637
7	29.147537662579424	31.385562700700554	20.9690158041847	18.497883832535322
8	26.83199103108935	34.46032194720635	19.831805547665475	18.87588147403882
9	26.927490235628245	15.724805046228694	19.253599205777483	38.09410551236558
10-11	25.857371070938704	25.148764301495653	27.59682170733644	21.397042920229207
12-13	26.27714580824982	22.515046685280087	27.455999611212683	23.75180789525741
14-15	23.647666967832635	24.128904967047742	24.558490064860504	27.66493800025912
16-17	24.00642883887312	27.13480642637467	25.31375841338009	23.545006321372124
18-19	23.987021278306894	26.67169402194754	26.429788910968412	22.911495788777156
20-21	24.523446911619757	25.752604451867267	26.375328551089694	23.34862008542328
22-23	24.387778871450035	25.445286349583867	26.119854498801153	24.047080280164945
24-25	24.235015169539952	25.70486974151282	26.010861962235232	24.049253126711996
26-27	23.998409207702952	25.950894555420955	26.06719215063105	23.983504086245038
28-29	24.108595005657772	26.150611900092624	25.90883260984916	23.831960484400444
30-31	24.46559599082571	25.7892805232869	25.82687080969682	23.918252676190566
32-33	24.184890239385158	25.80875364535614	25.80079780795293	24.20555830730577
34-35	24.355582991247402	25.91658298799664	25.862449204666248	23.865384816089712
36-37	24.391123918499797	25.86398122094964	25.889728679949165	23.855166180601405
38-39	24.327462648314764	25.913627173833266	25.85229182895155	23.906618348900423
40-41	24.35698693897229	25.7513516879697	25.903273836764782	23.988387536293228
42-43	24.188286279763666	25.771418824342195	25.76944736591725	24.270847529976884
44-45	24.129410577072548	25.745568834678544	25.91857011204978	24.206450476199127
46-47	24.2853575273457	25.72632343214185	25.748245585017244	24.2400734554952
48-49	24.104746206426807	25.879305300435846	25.81619691598478	24.19975157715257
50-51	24.12740146062967	25.8508142127734	25.94708688192758	24.07469744466935
52-53	24.308403338442844	25.701615143900202	25.91830231888872	24.07167919876823
54-55	23.94315574015649	25.609089103164766	26.132662773272735	24.315092383406004
56-57	24.020361485621876	25.72737406533249	25.8960817544914	24.35618269455423
58-59	24.101906849532973	25.737772021400378	26.00357975671916	24.156741372347494
60-61	24.213609158596384	25.67346830326494	26.154899723929038	23.958022814209638
62-63	24.159616798459048	25.66607991330518	26.095525778493833	24.07877750974194
64-65	23.99905240599398	25.547189255847297	26.03079131984692	24.42296701831181
66-67	24.027563931932775	25.705677005140814	25.935576405085037	24.33118265784137
68-69	23.995002694390323	25.609271582203586	26.10708247576763	24.28864324763847
70-71	23.960002597990602	25.492063018751978	26.07345767014739	24.474476713110032
72-73	23.92809810176425	25.72081251941999	25.935197183675506	24.41589219514026
74-75	23.940096458398997	25.768919047920193	25.986199809523715	24.3047846841571
76-77	23.973612830394465	25.502518698938143	25.985350020574273	24.538518450093118
78-79	23.907752115652528	25.53448221775343	26.072902865377014	24.48486280121703
80-81	24.006501629779123	25.70837059975495	25.915708197446975	24.36941957301896
82-83	24.019526244283774	25.717246610979437	26.035186669352456	24.228040475384333
84-85	23.998392030781122	25.756461233456392	25.941891969496584	24.3032547662659
86-87	23.825779981517393	25.888665428777664	25.942621615736623	24.342932973968328
88-89	23.804840764593298	25.618717307625378	26.085662444015018	24.490779483766307
90-91	23.915398670273273	25.55880909236953	26.086821598569564	24.438970638787634
92-93	23.991608921564676	25.51131312490516	26.16523086181725	24.33184709171291
94-95	23.964173318337245	25.455271262811134	26.09439407737961	24.48616134147201
96-97	23.833248624597633	25.388888545938542	26.212655279343345	24.56520755012048
98-99	23.69315796981776	25.330932681149683	26.150420142708608	24.82548920632395
100-101	23.95289096046176	25.37466045766389	26.166310026823865	24.506138555050487
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	43.0
1	36.0
2	42.5
3	63.0
4	61.0
5	76.0
6	132.5
7	255.0
8	374.5
9	454.0
10	440.5
11	389.0
12	441.5
13	509.0
14	555.5
15	636.0
16	742.5
17	814.5
18	899.5
19	1003.0
20	1083.0
21	1164.0
22	1285.0
23	1415.0
24	1576.0
25	1874.5
26	2325.5
27	3005.0
28	4185.0
29	6101.0
30	9151.0
31	13757.0
32	20240.5
33	26858.0
34	33245.0
35	40313.0
36	49364.5
37	58587.0
38	66549.0
39	74093.5
40	80985.5
41	87318.0
42	93717.5
43	101026.5
44	108065.5
45	114960.5
46	120232.0
47	124129.0
48	128083.0
49	130465.0
50	129976.0
51	128872.5
52	127656.0
53	125557.0
54	122600.0
55	116237.0
56	107474.5
57	97437.0
58	87054.0
59	75506.5
60	62877.0
61	52214.0
62	42665.5
63	32794.0
64	24078.0
65	18073.0
66	13816.0
67	10667.0
68	8282.0
69	6405.0
70	4895.5
71	3641.5
72	2686.5
73	2020.5
74	1540.5
75	1165.0
76	888.5
77	650.0
78	410.5
79	273.0
80	212.5
81	173.0
82	134.0
83	108.0
84	82.5
85	65.5
86	62.5
87	58.5
88	48.5
89	34.0
90	25.5
91	27.5
92	26.0
93	17.5
94	16.0
95	14.5
96	16.5
97	12.5
98	5.0
99	5.0
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04762779697830369
2	0.055634193884139226
3	0.04670398195070728
4	0.053683917714769026
5	0.046464474350960065
6	0.016081224554455986
7	0.013651933185591358
8	0.019468546322309484
9	0.019160607979777346
10-11	0.020221284492943595
12-13	0.025901036144091876
14-15	0.04240995284095361
16-17	0.028364542884348965
18-19	0.022222883719402477
20-21	0.025422020944597445
22-23	0.04490767495260316
24-25	0.040493892042975876
26-27	0.029031742626501926
28-29	0.01972516160775293
30-31	0.012505718243943965
32-33	0.009597411675584904
34-35	0.009357904075837687
36-37	0.00152258402696445
38-39	0.006997043449757977
40-41	0.0038834446530441585
42-43	0.2066779508961519
44-45	0.1828298370356076
46-47	0.18895438851485785
48-49	0.18679882011713292
50-51	0.1857210359182704
52-53	0.19004928039941657
54-55	0.18816743497283128
56-57	0.1978846004482898
58-59	0.19519869379398172
60-61	0.19983487661766
62-63	0.19723450839183304
64-65	0.19851758481905032
66-67	0.18878331165789555
68-69	0.18787660431599537
70-71	0.17188091819002055
72-73	0.17940829989636164
74-75	0.1792714384107918
76-77	0.17738959298420653
78-79	0.20157986055867544
80-81	0.21088644157742442
82-83	0.20787548889488797
84-85	0.20325641375690595
86-87	0.21925209988288077
88-89	0.21786637734148617
90-91	0.2172505006564219
92-93	0.22139056059490952
94-95	0.23237369481188902
96-97	0.23228815638340786
98-99	0.22277628313630413
100-101	0.2323052640691041
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2922663.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.15519700604907
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.53211298633191	50.22866263959145
2	11.434170234737397	14.442545475510851
3	3.7425097798382954	7.0907682732825865
4	1.724247133369013	4.355806695801415
5	0.9357666177201753	2.954926254690094
6	0.6116152589119966	2.317600930109571
7	0.40882919188342187	1.8073781708654941
8	0.30707013519438825	1.5514459906300568
9	0.22106070258133517	1.2565018999637858
>10	1.0362051197068443	11.112945847353974
>50	0.036472956609633046	1.539003150378238
>100	0.00912679794909335	0.9808725388848161
>500	7.588794887878891E-4	0.32508323970497666
>1k	5.420567774110333E-5	0.036458893232638664
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	3.421537139245955E-5	0.0	0.0	0.0
6	0.0	3.421537139245955E-5	0.0	0.0	0.0
7	0.0	3.421537139245955E-5	0.0	0.0	0.0
8	0.0	3.421537139245955E-5	0.0	0.0	0.0
9	0.0	3.421537139245955E-5	0.0	0.0	3.421537139245955E-5
10-11	0.0	3.421537139245955E-5	0.0	0.0	3.421537139245955E-5
12-13	0.0	3.421537139245955E-5	0.0	0.0	3.421537139245955E-5
14-15	0.0	5.132305708868932E-5	0.0	1.7107685696229774E-5	1.5396917126606795E-4
16-17	0.0	6.84307427849191E-5	0.0	8.553842848114887E-5	2.3950759974721684E-4
18-19	0.0	6.84307427849191E-5	0.0	2.052922283547573E-4	2.737229711396764E-4
20-21	0.0	6.84307427849191E-5	0.0	2.052922283547573E-4	2.9083065683590615E-4
22-23	0.0	6.84307427849191E-5	0.0	2.737229711396764E-4	4.105844567095146E-4
24-25	0.0	6.84307427849191E-5	0.0	4.790151994944337E-4	4.790151994944337E-4
26-27	0.0	6.84307427849191E-5	0.0	6.671997421529611E-4	4.790151994944337E-4
28-29	0.0	6.84307427849191E-5	0.0	0.0014712609698757607	4.790151994944337E-4
30-31	0.0	6.84307427849191E-5	0.0	0.005115198023172702	4.961228851906635E-4
32-33	0.0	1.0264611417737864E-4	0.0	0.016885285782178787	5.132305708868933E-4
34-35	0.0	1.0264611417737864E-4	0.0	0.03324023330777445	5.816613136718123E-4
36-37	0.0	1.0264611417737864E-4	0.0	0.05727653171097728	5.816613136718123E-4
38-39	0.0	1.0264611417737864E-4	0.0	0.09559774767053197	5.816613136718123E-4
40-41	0.0	1.0264611417737864E-4	0.0	0.14250702184959402	5.816613136718123E-4
42-43	0.0	1.0264611417737864E-4	0.0	0.19430909413777775	5.816613136718123E-4
44-45	0.0	1.0264611417737864E-4	0.0	0.26065269926775686	5.816613136718123E-4
46-47	0.0	1.0264611417737864E-4	0.0	0.33563568567433194	5.98768999368042E-4
48-49	0.0	1.0264611417737864E-4	0.0	0.41395467079167186	6.84307427849191E-4
50-51	0.0	1.0264611417737864E-4	0.0	0.49586626990522	6.84307427849191E-4
52-53	0.0	1.0264611417737864E-4	0.0	0.5856131890676415	7.185227992416505E-4
54-55	0.0	1.0264611417737864E-4	0.0	0.6911162867562903	7.869535420265696E-4
56-57	0.0	1.0264611417737864E-4	0.0	0.8138810393124353	8.040612277227994E-4
58-59	0.0	1.0264611417737864E-4	0.0	0.9460550190015065	8.211689134190292E-4
60-61	0.0	1.0264611417737864E-4	0.0	1.0827625354000787	8.211689134190292E-4
62-63	0.0	1.1975379987360842E-4	0.0	1.2284686944748677	8.553842848114887E-4
64-65	0.0	1.368614855698382E-4	0.0	1.390444262646771	8.553842848114887E-4
66-67	0.0	1.368614855698382E-4	0.0	1.5884315092092383	8.553842848114887E-4
68-69	0.0	1.368614855698382E-4	0.0	1.800293088871348	8.553842848114887E-4
70-71	0.0	1.368614855698382E-4	0.0	2.0210506650954967	8.895996562039482E-4
72-73	0.0	1.368614855698382E-4	0.0	2.2541428827066277	0.0010948918845587055
74-75	0.0	1.368614855698382E-4	0.0	2.5079354000101963	0.0010948918845587055
76-77	0.0	1.368614855698382E-4	0.0	2.7940443355939433	0.0011119995702549353
78-79	0.0	1.368614855698382E-4	0.0	3.1112037207163468	0.001129107255951165
80-81	0.0	1.368614855698382E-4	0.0	3.4430928232232043	0.0011462149416473948
82-83	0.0	1.368614855698382E-4	0.0	3.789899827657174	0.0011633226273436246
84-85	0.0	1.368614855698382E-4	0.0	4.157954577725862	0.0011633226273436246
86-87	0.0	1.7107685696229774E-4	0.0	4.564176574582838	0.0011633226273436246
88-89	0.0	1.7107685696229774E-4	0.0	5.005469327117085	0.0011633226273436246
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2340	0.0	44.41568	1
GTATCAA	4120	0.0	38.588303	1
TCAACGC	4895	0.0	32.096504	4
CAACGCA	5010	0.0	31.547081	5
ATCAACG	5030	0.0	31.044209	3
TATCAAC	5115	0.0	30.900017	2
AACGCAG	5470	0.0	29.057217	6
GTACATG	8615	0.0	28.094606	1
TACATGG	8890	0.0	25.840666	2
ACATGGG	9370	0.0	24.009962	3
ACGCAGA	6470	0.0	23.98401	7
CGCAGAG	6835	0.0	22.636896	8
CATGGGG	8070	0.0	22.115498	4
GAGTACA	6435	0.0	18.511143	1
GCAGAGT	7780	0.0	18.483574	9
ATGGGGG	5270	0.0	18.46273	5
CTATACG	190	1.8771757E-4	17.487465	4
AGAGTAC	6305	0.0	17.463581	10-11
AGTACAT	6605	0.0	16.743383	2
TATACTG	1125	0.0	16.453743	5
>>END_MODULE