FastQCFastQC Report
Mon 15 Aug 2016
SRR2921361_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921361_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2922663
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22450.049.540391
GTATCAA39500.040.67051
TCAACGC50200.031.295494
ATCAACG51000.030.8045773
TATCAAC51850.030.2995822
CAACGCA51900.030.0484965
AACGCAG56100.027.8802816
GTACATG86350.024.053541
TACATGG89650.022.659532
ACGCAGA67950.022.38817
CGCAGAG71550.021.3280968
ACATGGG93300.021.2643493
CATGGGG79850.020.6852974
GCAGAGT82850.017.6731879
AGAGTAC68400.017.54938910-11
ATGGGGG50200.017.5220245
ATACCGT3301.1994416E-817.2874686
GAGTACA66550.016.2834591
TATACCG2406.1015307E-515.8487495
CAGAGTA75950.015.71095710-11
Produced by FastQC (version 0.11.5)