##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921361_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2922663
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.405266361533986	33.0	31.0	34.0	29.0	34.0
2	31.53799360377847	34.0	31.0	34.0	29.0	34.0
3	31.6098670972329	34.0	31.0	34.0	28.0	34.0
4	35.09013389501287	37.0	35.0	37.0	33.0	37.0
5	34.821653403077946	37.0	35.0	37.0	32.0	37.0
6	34.77974881127246	37.0	35.0	37.0	32.0	37.0
7	34.64465283886647	37.0	35.0	37.0	32.0	37.0
8	34.609793876338124	37.0	35.0	37.0	32.0	37.0
9	36.23382134717551	39.0	37.0	39.0	32.0	39.0
10-11	36.08185480159703	39.0	37.0	39.0	32.0	39.0
12-13	36.017453089870436	39.0	37.0	39.0	31.5	39.0
14-15	37.26887140255308	40.0	38.0	41.0	31.0	41.0
16-17	37.243944991263106	40.0	38.0	41.0	31.0	41.0
18-19	37.2038582621397	40.0	38.0	41.0	31.0	41.0
20-21	37.16693731025438	40.0	38.0	41.0	31.0	41.0
22-23	37.05635374314453	40.0	38.0	41.0	31.0	41.0
24-25	36.98907828237467	40.0	37.5	41.0	31.0	41.0
26-27	36.85598356704143	40.0	37.0	41.0	30.0	41.0
28-29	36.70629319904484	40.0	37.0	41.0	30.0	41.0
30-31	36.577471983598514	40.0	37.0	41.0	30.0	41.0
32-33	36.53746873998131	40.0	37.0	41.0	30.0	41.0
34-35	36.67288941626181	40.0	37.0	41.0	30.0	41.0
36-37	36.658989763787346	40.0	37.0	41.0	30.0	41.0
38-39	36.57712555296317	40.0	37.0	41.0	30.0	41.0
40-41	36.46714947977239	40.0	37.0	41.0	30.0	41.0
42-43	36.37152538626589	40.0	37.0	41.0	29.5	41.0
44-45	36.24693712549137	40.0	36.0	41.0	29.0	41.0
46-47	36.101373473438436	40.0	36.0	41.0	28.5	41.0
48-49	35.9561711357074	40.0	36.0	41.0	28.0	41.0
50-51	35.808280496246056	40.0	35.0	41.0	28.0	41.0
52-53	35.640334516843026	39.0	35.0	41.0	27.5	41.0
54-55	35.440163987432015	39.0	35.0	41.0	27.0	41.0
56-57	35.17758410737057	39.0	35.0	41.0	26.0	41.0
58-59	34.952528225115245	38.5	35.0	41.0	26.0	41.0
60-61	34.6785431642307	38.0	35.0	40.0	26.0	41.0
62-63	34.3838364190466	37.5	34.0	40.0	26.0	41.0
64-65	34.070822568322114	37.0	34.0	40.0	25.5	41.0
66-67	33.73401859879158	36.5	34.0	39.5	24.5	41.0
68-69	33.36854077941932	36.0	34.0	39.0	23.5	41.0
70-71	32.99061865839476	35.5	33.5	39.0	23.0	40.0
72-73	32.592938529005906	35.0	33.0	37.5	22.0	40.0
74-75	32.18779585603951	35.0	33.0	37.0	21.5	39.0
76-77	29.45573471864529	33.0	29.0	34.5	15.0	36.5
78-79	31.106452745321647	34.5	31.5	36.0	19.0	37.5
80-81	31.299092642566045	35.0	32.0	36.0	20.0	37.0
82-83	31.14175377044839	35.0	32.5	35.0	19.5	37.0
84-85	30.920348326166923	35.0	32.0	35.0	18.0	36.5
86-87	30.683362741445045	35.0	32.0	35.0	16.0	36.0
88-89	30.41524065552546	35.0	32.0	35.0	10.0	36.0
90-91	30.242885512287934	35.0	32.0	35.0	4.5	35.0
92-93	30.11130995944452	34.0	32.0	35.0	3.5	35.0
94-95	30.02280420287936	34.0	32.0	35.0	2.0	35.0
96-97	29.846495986708014	34.0	31.5	35.0	2.0	35.0
98-99	29.597722009003434	34.0	31.0	35.0	2.0	35.0
100-101	28.53524645160937	33.5	29.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	57402.0
3	19550.0
4	10373.0
5	7178.0
6	6176.0
7	6260.0
8	6402.0
9	6787.0
10	7473.0
11	8105.0
12	8554.0
13	9242.0
14	9690.0
15	10772.0
16	11561.0
17	11905.0
18	11282.0
19	10400.0
20	9937.0
21	9824.0
22	10311.0
23	11836.0
24	13991.0
25	16657.0
26	20383.0
27	24840.0
28	29634.0
29	35888.0
30	44332.0
31	54341.0
32	69946.0
33	92947.0
34	134677.0
35	202231.0
36	355628.0
37	708198.0
38	762102.0
39	95846.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.25842348584218	20.141804311147112	14.261160613324892	25.33861158968581
2	15.180385765946214	21.07349042120293	37.65404240599209	26.092081406858767
3	17.753674266583815	25.090813033278494	29.019999397773404	28.135513302364284
4	13.412077891415999	17.222132878020073	35.20059674323275	34.16519248733118
5	14.628602015259903	37.28894769373142	33.826310634223006	14.256139656785674
6	33.5306332701823	35.355637726795415	17.004930160338848	14.10879884268344
7	28.987023136091985	31.68247587901855	21.037492177510718	18.29300880737875
8	26.861467166036977	35.02918962271446	19.544147807408027	18.565195403840534
9	26.666434002141198	15.878190540613133	19.236634535011394	38.21874092223428
10-11	25.7469920389918	25.506581201718546	27.530405831782286	21.216020927507373
12-13	26.252939319780026	22.70638964709344	27.53302431092251	23.50764672220402
14-15	23.55356602104694	24.370738901254573	24.559565990251546	27.516129087446945
16-17	23.91786933820561	27.43379318839687	25.289582093024848	23.358755380372674
18-19	23.964926405088253	26.886807865931466	26.48044782576935	22.667817903210935
20-21	24.478228944906697	25.886595073909152	26.43291583437178	23.20226014681237
22-23	24.340805978115824	25.58621255979142	26.136926635325352	23.936054826767396
24-25	24.166046502874995	25.921375206792547	25.993776121726604	23.918802168605854
26-27	23.930192115390703	26.160626224832917	26.08911437362547	23.82006728615091
28-29	24.04876069170091	26.356340871117208	25.922359696928325	23.67253874025356
30-31	24.45019497881848	25.953972645304173	25.864606284116586	23.731226091760757
32-33	24.18487220406559	25.982438571015244	25.773285130856927	24.05940409406224
34-35	24.308717145532594	26.09834555442499	25.917617004327187	23.67532029571523
36-37	24.32169296021	26.043387425906133	25.987376498814086	23.64754311506978
38-39	24.30849321208523	26.06809042797523	25.863499229725	23.75991713021454
40-41	24.36580217041362	25.926555770760988	25.87848099446695	23.829161064358438
42-43	24.231613367268658	25.94353245356078	25.72843689241321	24.096417286757355
44-45	24.164092603979643	25.903180340423	25.85274274121166	24.0799843143857
46-47	24.23975311983567	25.84143900051653	25.747303704725194	24.17150417492261
48-49	24.06356716130111	26.06086280913512	25.81385071106873	24.061719318495044
50-51	24.14573314819542	26.006721628629677	25.861691986261103	23.985853236913798
52-53	24.348286067246843	25.81881568270667	25.78448005822172	24.04841819182477
54-55	23.957693750670412	25.74889872740122	26.040792208007723	24.252615313920643
56-57	24.064525555337706	25.92255742598548	25.775457390775912	24.237459627900897
58-59	24.129212401803656	25.852232627491638	25.926452510310455	24.092102460394248
60-61	24.198942667966946	25.902838371913976	25.990487433489022	23.90773152663005
62-63	24.158682802763998	25.87197961496764	25.930241630802588	24.03909595146577
64-65	23.948549869716395	25.70247557674411	25.924503619977802	24.424470933561693
66-67	23.973651716976725	25.86095273789568	25.81390712213121	24.35148842299639
68-69	23.908978217553408	25.79806112062929	25.942607774272442	24.35035288754486
70-71	23.878414261277044	25.68178259117176	25.926538883150734	24.513264264400462
72-73	23.867162594077275	25.874617932717825	25.78195667046438	24.476262802740514
74-75	23.844583380604597	25.902290769689102	25.802991171524788	24.450134678181517
76-77	23.785149136254667	25.5773220979563	25.929336474849013	24.70819229094002
78-79	23.670833917515377	25.664944677746355	25.981592744741462	24.682628659996805
80-81	23.838497847377415	25.74966930130143	25.81409735002848	24.597735501292668
82-83	23.78234571325563	25.773373690382222	25.92975183631816	24.514528760043987
84-85	23.677471986150643	25.756586563859802	25.888186925221728	24.67775452476782
86-87	23.549089295320616	25.872412656005967	25.839608043088415	24.738890005585002
88-89	23.470850654933148	25.648723787751027	25.940769562744386	24.939655994571442
90-91	23.460094949792076	25.573579910583195	25.993668297674105	24.972656841950624
92-93	23.50162604409147	25.511810214980144	26.04087361358346	24.94569012734493
94-95	23.41843530937033	25.47148312782097	25.994026414757336	25.116055148051363
96-97	23.233127885474794	25.405095681762496	26.104094037905885	25.257682394856822
98-99	23.049847893901077	25.345057244653873	26.024821470791217	25.580273390653836
100-101	23.044765446101916	25.3991577769623	26.094731700724267	25.461345076211515
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	31.0
1	25.5
2	31.5
3	55.0
4	54.5
5	48.5
6	83.0
7	204.5
8	320.0
9	343.0
10	305.5
11	251.5
12	257.0
13	300.0
14	336.5
15	373.5
16	395.0
17	428.5
18	505.0
19	564.5
20	641.0
21	760.5
22	891.5
23	1054.0
24	1304.0
25	1676.0
26	2207.5
27	3038.5
28	4448.0
29	6750.5
30	10125.5
31	15157.5
32	21856.5
33	28112.5
34	34280.0
35	41248.0
36	49698.0
37	58304.5
38	66393.0
39	74254.0
40	81281.5
41	87740.0
42	93847.0
43	100983.5
44	108332.5
45	115319.5
46	120710.5
47	125190.0
48	129352.0
49	131536.5
50	131239.0
51	130368.0
52	128926.0
53	126325.5
54	123006.0
55	116523.0
56	107555.0
57	97363.5
58	86585.5
59	74869.5
60	62174.0
61	51208.5
62	41626.0
63	31750.5
64	23071.0
65	16892.0
66	12676.0
67	9868.5
68	7626.0
69	5811.5
70	4470.5
71	3323.0
72	2361.5
73	1707.0
74	1286.5
75	952.0
76	669.0
77	411.5
78	245.5
79	154.5
80	87.0
81	43.5
82	29.5
83	23.0
84	14.0
85	7.5
86	6.0
87	6.0
88	4.0
89	2.5
90	1.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14627071270276457
2	3.421537139245955E-4
3	0.005987689993680421
4	0.00455064439519712
5	0.025901036144091876
6	0.001368614855698382
7	0.0
8	0.0016765531982305178
9	0.0
10-11	0.09818100821066267
12-13	1.8818454265852753E-4
14-15	0.0050638749660840135
16-17	1.0264611417737864E-4
18-19	2.908306568359062E-4
20-21	7.185227992416505E-4
22-23	1.0264611417737864E-4
24-25	0.0016423378268380583
26-27	0.002412183683168398
28-29	0.006364059078997475
30-31	0.014455994413314159
32-33	0.013908548471034806
34-35	0.011102888016853123
36-37	6.500920564567314E-4
38-39	1.8818454265852753E-4
40-41	0.004533536709500891
42-43	0.008245904505582751
44-45	0.008211689134190292
46-47	0.009357904075837687
48-49	0.011513472473562639
50-51	0.00537181330861615
52-53	0.0014541532841795307
54-55	7.356304849378802E-4
56-57	0.005542890165578447
58-59	0.00908418110469801
60-61	0.0072878741065938845
62-63	0.017757777752686506
64-65	0.03284675653676117
66-67	0.03525894021992956
68-69	0.02631162060080139
70-71	0.040562322785760795
72-73	0.02497722111649547
74-75	0.023865221546240534
76-77	0.23516224758037446
78-79	0.06543689778807889
80-81	0.05388920994312379
82-83	0.01982780772193031
84-85	0.032111126051823285
86-87	0.08013239980114026
88-89	0.26376629806447066
90-91	0.3157565548953129
92-93	0.050057088347168315
94-95	0.004447998281019741
96-97	0.0091355041617867
98-99	0.0010948918845587055
100-101	0.0013857225413946117
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2922663.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.042079249187324
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.62146096627617	48.77838911956594
2	11.879711649950133	14.740840232874
3	3.97313402319177	7.395044878035187
4	1.7592994758270384	4.366023900092594
5	1.0166885286453335	3.153873513297672
6	0.6515209150898296	2.4253027347903746
7	0.43051810757605113	1.8697166983910465
8	0.2906673079423964	1.4426883323608075
9	0.2274464251943735	1.2700124223168308
>10	1.101962014201178	11.641422665862484
>50	0.03735349105129397	1.564683749016737
>100	0.009574255551801204	1.0396654219176704
>500	5.523662522505115E-4	0.23800480824638315
>1k	1.1047325038195341E-4	0.0743315232321947
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.0264611417737864E-4	0.0
5	0.0	0.0	0.0	1.0264611417737864E-4	3.421537139245955E-5
6	0.0	0.0	0.0	1.0264611417737864E-4	3.421537139245955E-5
7	0.0	0.0	0.0	1.0264611417737864E-4	3.421537139245955E-5
8	0.0	0.0	0.0	1.0264611417737864E-4	3.421537139245955E-5
9	0.0	0.0	0.0	1.0264611417737864E-4	3.421537139245955E-5
10-11	0.0	0.0	0.0	1.0264611417737864E-4	3.421537139245955E-5
12-13	0.0	3.421537139245955E-5	0.0	1.7107685696229774E-4	3.421537139245955E-5
14-15	0.0	3.421537139245955E-5	0.0	1.7107685696229774E-4	8.553842848114887E-5
16-17	0.0	3.421537139245955E-5	0.0	2.052922283547573E-4	1.368614855698382E-4
18-19	0.0	3.421537139245955E-5	0.0	2.3950759974721684E-4	2.052922283547573E-4
20-21	0.0	3.421537139245955E-5	0.0	2.3950759974721684E-4	2.052922283547573E-4
22-23	0.0	3.421537139245955E-5	0.0	3.2504602822836567E-4	2.566152854434466E-4
24-25	0.0	3.421537139245955E-5	0.0	4.790151994944337E-4	3.079383425321359E-4
26-27	0.0	3.421537139245955E-5	0.0	6.671997421529611E-4	3.079383425321359E-4
28-29	0.0	3.421537139245955E-5	0.0	0.0014883686555719903	3.079383425321359E-4
30-31	0.0	3.421537139245955E-5	0.0	0.005269167194438771	3.079383425321359E-4
32-33	0.0	3.421537139245955E-5	0.0	0.017141901067622235	3.76369085317055E-4
34-35	0.0	3.421537139245955E-5	0.0	0.033599494707395275	3.76369085317055E-4
36-37	0.0	5.132305708868932E-5	0.0	0.05778976228186418	3.76369085317055E-4
38-39	0.0	6.84307427849191E-5	0.0	0.0965215626981284	4.2769214240574434E-4
40-41	0.0	6.84307427849191E-5	0.0	0.14372166753402632	4.790151994944337E-4
42-43	0.0	6.84307427849191E-5	0.0	0.19569481667917238	4.790151994944337E-4
44-45	0.0	6.84307427849191E-5	0.0	0.262449006265861	5.132305708868933E-4
46-47	0.0	6.84307427849191E-5	0.0	0.33772282332927195	5.816613136718123E-4
48-49	0.0	6.84307427849191E-5	0.0	0.415904946961042	6.84307427849191E-4
50-51	0.0	6.84307427849191E-5	0.0	0.49762836153193163	6.84307427849191E-4
52-53	0.0	6.84307427849191E-5	0.0	0.5869646962376436	7.356304849378804E-4
54-55	0.0	6.84307427849191E-5	0.0	0.692348040126419	8.040612277227994E-4
56-57	0.0	6.84307427849191E-5	0.0	0.8151641157396525	8.211689134190292E-4
58-59	0.0	6.84307427849191E-5	0.0	0.947132803200369	8.553842848114887E-4
60-61	0.0	6.84307427849191E-5	0.0	1.0834126274565354	8.553842848114887E-4
62-63	0.0	6.84307427849191E-5	0.0	1.2286397713318298	9.238150275964078E-4
64-65	0.0	6.84307427849191E-5	0.0	1.3907179856179108	9.238150275964078E-4
66-67	0.0	6.84307427849191E-5	0.0	1.589167139694176	9.409227132926376E-4
68-69	0.0	6.84307427849191E-5	0.0	1.7998653967289422	9.580303989888674E-4
70-71	0.0	6.84307427849191E-5	0.0	2.021649434094865	9.751380846850972E-4
72-73	0.0	6.84307427849191E-5	0.0	2.2549469439343506	0.001129107255951165
74-75	0.0	6.84307427849191E-5	0.0	2.5107239527786818	0.001129107255951165
76-77	0.0	6.84307427849191E-5	0.0	2.7997583026164836	0.001129107255951165
78-79	0.0	6.84307427849191E-5	0.0	3.1191074715080047	0.001129107255951165
80-81	0.0	6.84307427849191E-5	0.0	3.4539733113260063	0.0011462149416473948
82-83	0.0	6.84307427849191E-5	0.0	3.803278037871626	0.0012317533701285436
84-85	0.0	6.84307427849191E-5	0.0	4.173796294680571	0.0012317533701285436
86-87	0.0	6.84307427849191E-5	0.0	4.583747767019325	0.0012317533701285436
88-89	0.0	6.84307427849191E-5	0.0	5.027692210836488	0.0012317533701285436
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2245	0.0	49.54039	1
GTATCAA	3950	0.0	40.6705	1
TCAACGC	5020	0.0	31.29549	4
ATCAACG	5100	0.0	30.804577	3
TATCAAC	5185	0.0	30.299582	2
CAACGCA	5190	0.0	30.048496	5
AACGCAG	5610	0.0	27.880281	6
GTACATG	8635	0.0	24.05354	1
TACATGG	8965	0.0	22.65953	2
ACGCAGA	6795	0.0	22.3881	7
CGCAGAG	7155	0.0	21.328096	8
ACATGGG	9330	0.0	21.264349	3
CATGGGG	7985	0.0	20.685297	4
GCAGAGT	8285	0.0	17.673187	9
AGAGTAC	6840	0.0	17.549389	10-11
ATGGGGG	5020	0.0	17.522024	5
ATACCGT	330	1.1994416E-8	17.287468	6
GAGTACA	6655	0.0	16.283459	1
TATACCG	240	6.1015307E-5	15.848749	5
CAGAGTA	7595	0.0	15.710957	10-11
>>END_MODULE