FastQCFastQC Report
Mon 15 Aug 2016
SRR2921360_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921360_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2614152
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30300.056.229711
GTATCAA50650.040.759031
TATCAAC62500.032.1217582
TCAACGC63150.031.7147624
ATCAACG63500.031.542373
CAACGCA64000.031.4394535
AACGCAG67300.030.1718186
ACGCAGA80250.024.420397
CGCAGAG80700.023.8151868
GTACATG75150.023.8081911
TACATGG75900.022.8864442
GTGGTAT13250.021.1324671
ACATGGG79450.021.0880473
GCAGAGT89800.019.9750549
CATGGGG67800.018.7597684
GAGTACT41250.018.63682412-13
TGGTATC14350.018.1907142
CAGAGTA85550.018.13862610-11
GTATAGG5250.018.079431
GAGTACA61200.017.8357121
Produced by FastQC (version 0.11.5)