##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921360_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2614152
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.50906335974343	31.0	31.0	34.0	27.0	34.0
2	30.563314604506548	33.0	31.0	34.0	26.0	34.0
3	30.60414620113903	33.0	31.0	34.0	26.0	34.0
4	34.073245167075214	37.0	35.0	37.0	30.0	37.0
5	34.06925840578513	37.0	35.0	37.0	30.0	37.0
6	34.11928610119075	37.0	35.0	37.0	30.0	37.0
7	34.04467108263024	37.0	35.0	37.0	30.0	37.0
8	34.011744152597096	37.0	35.0	37.0	30.0	37.0
9	35.57843231763111	39.0	37.0	39.0	30.0	39.0
10-11	35.47744545841252	39.0	37.0	39.0	30.0	39.0
12-13	35.36839059090673	39.0	35.0	39.0	30.0	39.0
14-15	36.50713883507922	40.0	37.0	41.0	29.0	41.0
16-17	36.45545936120011	40.0	37.0	41.0	29.0	41.0
18-19	36.41204853428569	40.0	37.0	41.0	28.5	41.0
20-21	36.3564735332911	40.0	37.0	41.0	28.0	41.0
22-23	36.2418193356775	40.0	37.0	41.0	27.5	41.0
24-25	36.14153920659548	40.0	36.0	41.0	27.0	41.0
26-27	35.99267907910481	40.0	36.0	41.0	27.0	41.0
28-29	35.851507486940314	40.0	36.0	41.0	27.0	41.0
30-31	35.69944058340907	40.0	36.0	41.0	26.5	41.0
32-33	35.5071457206773	39.5	35.0	41.0	25.5	41.0
34-35	35.34180185390903	39.0	35.0	41.0	25.0	41.0
36-37	35.169070122930876	39.0	35.0	41.0	25.0	41.0
38-39	35.38853555569837	39.5	35.0	41.0	25.0	41.0
40-41	35.500154352157026	40.0	35.0	41.0	25.0	41.0
42-43	35.322952720423295	40.0	35.0	41.0	24.0	41.0
44-45	35.192724447545515	40.0	35.0	41.0	24.0	41.0
46-47	35.023428630010805	40.0	35.0	41.0	23.0	41.0
48-49	34.868323265058805	39.5	35.0	41.0	23.0	41.0
50-51	33.139466067772645	37.0	32.5	39.5	18.5	40.0
52-53	33.2930298238205	37.5	33.5	39.5	19.0	40.0
54-55	34.152917083627884	39.0	34.0	40.5	19.5	41.0
56-57	34.16633596669207	39.0	34.0	41.0	20.0	41.0
58-59	33.9809745186967	38.0	34.0	41.0	18.5	41.0
60-61	33.73837940563517	38.0	34.0	40.0	18.0	41.0
62-63	33.44747130235732	37.0	34.0	40.0	15.0	41.0
64-65	33.15726323488458	37.0	33.0	40.0	13.0	41.0
66-67	32.8150101830345	36.0	33.0	39.5	9.5	41.0
68-69	32.4518669916669	36.0	33.0	39.0	7.5	41.0
70-71	32.07227181127953	35.0	32.5	39.0	6.0	40.5
72-73	31.71702066291478	35.0	32.0	37.5	2.0	40.0
74-75	31.329152245164014	35.0	32.0	37.0	2.0	39.0
76-77	30.955062482977272	35.0	32.0	36.5	2.0	39.0
78-79	30.615404154004814	35.0	31.5	36.0	2.0	38.5
80-81	30.275054587491468	35.0	31.0	36.0	2.0	37.0
82-83	29.981116247257237	35.0	31.0	35.0	2.0	37.0
84-85	29.749255016540737	35.0	31.0	35.0	2.0	36.5
86-87	29.525427366121022	35.0	31.0	35.0	2.0	36.0
88-89	29.323526711530164	35.0	31.0	35.0	2.0	36.0
90-91	29.07084668374295	35.0	30.0	35.0	2.0	35.5
92-93	28.881714414464042	34.0	30.0	35.0	2.0	35.0
94-95	28.67811416474635	34.0	30.0	35.0	2.0	35.0
96-97	28.430413954506086	34.0	30.0	35.0	2.0	35.0
98-99	28.193679059213082	34.0	30.0	35.0	2.0	35.0
100-101	27.14574821969036	33.5	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	83516.0
3	14417.0
4	9939.0
5	7757.0
6	7100.0
7	7516.0
8	7918.0
9	8348.0
10	9277.0
11	9845.0
12	10010.0
13	9989.0
14	11149.0
15	12453.0
16	12645.0
17	13364.0
18	12358.0
19	10844.0
20	10738.0
21	11352.0
22	12544.0
23	14200.0
24	16823.0
25	19379.0
26	23284.0
27	27752.0
28	32864.0
29	38781.0
30	46654.0
31	56450.0
32	71218.0
33	91284.0
34	128151.0
35	187365.0
36	311083.0
37	558991.0
38	588638.0
39	108141.0
40	15.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.69935851729891	18.125703971865235	13.479135642150553	24.6958018686853
2	16.64834563368702	19.59889951321679	38.384873359049124	25.36788149404707
3	19.70494536280384	23.16588795777214	29.155159533264545	27.974007146159472
4	14.272023135856177	16.12012059366007	35.214933282709616	34.39292298777414
5	15.15220519571973	36.2393414264501	33.536694579244674	15.071758798585492
6	33.78886402869771	33.99519766402625	17.04354852532475	15.172389781951297
7	29.068426161181826	31.489401751715235	20.529837809671122	18.912334277431814
8	26.899955617453053	33.70751136346245	19.83429240446274	19.558240614621752
9	26.660075580676846	16.046565104255343	19.59920891663743	37.69415039843038
10-11	25.848907818763458	25.09786223950432	27.250219331556234	21.803010610175992
12-13	26.42370066419117	22.812250870917488	26.983796888401073	23.78025157649027
14-15	23.855877604113847	24.490332866569794	24.43721443581376	27.2165750935026
16-17	23.80639800629328	27.352933805026243	25.485766084218653	23.354902104461832
18-19	23.783032095303923	26.49316998877591	26.943970051754775	22.779827864165394
20-21	24.201099140347203	25.602669526691628	27.03462066342529	23.161610669535875
22-23	24.17444264401855	25.436114264195194	26.633261409848284	23.756181681937967
24-25	24.042316739906543	25.765364013588442	26.52610708418306	23.66621216232196
26-27	23.673018565524035	26.048002087729405	26.56499827521256	23.713981071534004
28-29	23.73750977562361	26.23410074210566	26.520597296341812	23.507792185928917
30-31	24.123874547695763	25.712305047214095	26.510116541269962	23.653703863820176
32-33	23.828061039451917	25.778659298921617	26.348054030810474	24.045225630815985
34-35	24.19582003354731	25.95704421767992	26.27931874745234	23.567817001320428
36-37	24.141887656842663	25.872084516250087	26.363472742021276	23.62255508488597
38-39	23.909012919866985	25.991666021103693	26.45421428291343	23.64510677611589
40-41	24.108705311416863	25.707281328606875	26.494213157113037	23.68980020286323
42-43	24.00111473339836	25.749862335408636	26.249690697898625	23.99933223329438
44-45	23.974738925333163	25.871235740009528	26.2733917114518	23.88063362320551
46-47	24.094197691126347	25.89641228123631	26.022372794828662	23.98701723280868
48-49	23.82272073291599	26.054808496416904	26.1745389572412	23.947931813425907
50-51	23.928424902121563	26.0554688229082	26.294466397481404	23.721639877488833
52-53	24.20211293017526	25.788123261989504	26.20184425777909	23.807919550056138
54-55	23.735438833711616	25.77637835231641	26.39764967043109	24.09053314354089
56-57	23.765482318529603	25.964471280750708	26.17534531269554	24.094701088024145
58-59	23.80627405030578	25.88432395822973	26.34463715671749	23.964764834747008
60-61	23.780897056766065	25.842579052576504	26.58684907038118	23.78967482027625
62-63	23.821419748721077	25.78950364200544	26.561428261778232	23.827648347495252
64-65	23.599603282977103	25.72970538490655	26.488488977687062	24.18220235442929
66-67	23.665745611253037	25.89113021528704	26.259584319687928	24.183539853771993
68-69	23.708807323661993	25.819158576820783	26.447457857075374	24.02457624244185
70-71	23.615406133483546	25.68724828614237	26.437877690536133	24.259467889837946
72-73	23.5641864865129	25.828595495402105	26.40068092268703	24.206537095397966
74-75	23.614123452304923	25.953239522657373	26.387342399386526	24.045294625651177
76-77	23.665332797844147	25.652665770437626	26.382876557989952	24.299124873728278
78-79	23.57396106306768	25.739259895849003	26.40097960429062	24.285799436792697
80-81	23.64747581465029	25.961625451774207	26.235756749587136	24.155141983988372
82-83	23.630044433063475	25.900573593243692	26.446547112809228	24.02283486088361
84-85	23.63035628072447	25.937246707885304	26.378968031504485	24.053428979885744
86-87	23.56267251044863	26.11516102189252	26.200866330963578	24.12130013669527
88-89	23.439480067895573	25.949373518618092	26.287586247216943	24.323560166269395
90-91	23.47461550183875	25.8085267684711	26.372179460269724	24.344678269420427
92-93	23.53019770910626	25.71636915156727	26.541112368196167	24.212320771130308
94-95	23.491818889243135	25.635354836879117	26.57157137102982	24.301254902847933
96-97	23.583549554820824	25.509278767938206	26.518001373439397	24.389170303801574
98-99	23.352663916985072	25.569565947501477	26.480139186336555	24.597630949176896
100-101	23.56496001138013	25.475272851851834	26.56135061594763	24.398416520820405
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	79.0
1	61.5
2	64.5
3	93.5
4	96.0
5	101.0
6	177.0
7	385.0
8	620.5
9	730.5
10	681.5
11	576.5
12	552.5
13	614.0
14	694.0
15	784.5
16	880.5
17	970.5
18	1094.0
19	1262.0
20	1415.0
21	1548.0
22	1696.5
23	1960.0
24	2336.0
25	2854.0
26	3641.5
27	4868.5
28	6711.0
29	9505.5
30	13444.0
31	18808.0
32	25802.5
33	30764.5
34	34437.5
35	39383.0
36	45840.5
37	52705.0
38	58944.0
39	64811.5
40	69816.0
41	75444.0
42	81231.5
43	87141.0
44	92947.0
45	99715.5
46	104040.5
47	106953.5
48	110558.0
49	112118.5
50	113013.0
51	113343.0
52	112913.0
53	111421.5
54	108804.5
55	103077.5
56	94564.5
57	84694.5
58	75856.0
59	66695.0
60	55403.0
61	45915.5
62	38416.0
63	29606.0
64	21227.5
65	15974.5
66	12196.5
67	9491.5
68	7460.5
69	5783.0
70	4435.0
71	3246.0
72	2352.0
73	1720.0
74	1259.0
75	929.0
76	650.0
77	444.5
78	320.5
79	212.5
80	142.5
81	111.0
82	88.0
83	74.5
84	71.0
85	53.0
86	38.5
87	39.5
88	32.0
89	25.5
90	21.5
91	15.0
92	17.0
93	21.0
94	16.0
95	12.0
96	10.0
97	5.5
98	3.0
99	3.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04969106616600718
2	0.056997450798576356
3	0.04896425303501862
4	0.05397543830657131
5	0.047128093546205424
6	0.01621940881784992
7	0.01419198271561868
8	0.019585701214007448
9	0.019929981118159926
10-11	0.02023600769962879
12-13	0.02572535950472658
14-15	0.04295848137369212
16-17	0.027867545575008645
18-19	0.022053040527100186
20-21	0.025055926357763436
22-23	0.045521453993493874
24-25	0.04012773549510511
26-27	0.030086238290657926
28-29	0.019432687923273014
30-31	0.012011543322653006
32-33	0.009027784153331558
34-35	0.00885564420125532
36-37	0.0010519663737992282
38-39	0.005910138354617483
40-41	0.0028881258626124264
42-43	0.2087292552231087
44-45	0.18518433511134777
46-47	0.1913048667407251
48-49	0.18965997386532996
50-51	0.18822547426469463
52-53	0.1922803264691571
54-55	0.19073106690047095
56-57	0.20077256410491814
58-59	0.19767404496754587
60-61	0.20224531702823706
62-63	0.19962496442440988
64-65	0.20042828420076567
66-67	0.19155351333816856
68-69	0.189946873785457
70-71	0.17374659162894887
72-73	0.1817606627311648
74-75	0.1816076494404304
76-77	0.179790616612959
78-79	0.20440662975986096
80-81	0.21222943424865884
82-83	0.2107375546639981
84-85	0.20582200269915443
86-87	0.2216971316128519
88-89	0.21936367892915176
90-91	0.21878987908889763
92-93	0.22282560463201834
94-95	0.23340264835403604
96-97	0.23338352169269425
98-99	0.22378193769910854
100-101	0.2332496350633016
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2614152.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.69573776495035
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.80685820501853	48.62047257896449
2	11.975133480263697	14.776293897972526
3	3.798426654814017	7.030402044444095
4	1.751457855225792	4.322299381694913
5	0.9745353584090064	3.0062338957536983
6	0.6060533635889075	2.2434545634928407
7	0.4282501256763836	1.8494845216076035
8	0.294988166298604	1.4559610041377793
9	0.22102515722938454	1.2272679125892927
>10	1.0911891158587117	11.413721521939111
>50	0.037049530625519154	1.5549365177781638
>100	0.013229709095877179	1.4953038450837488
>500	0.0012436399279615261	0.5524364366574143
>1k	5.596379675701428E-4	0.45173187788426816
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	3.8253322683608296E-5
9	0.0	0.0	0.0	0.0	3.8253322683608296E-5
10-11	0.0	0.0	0.0	0.0	7.650664536721659E-5
12-13	0.0	0.0	0.0	0.0	1.5301329073443318E-4
14-15	0.0	0.0	0.0	0.0	1.5301329073443318E-4
16-17	0.0	0.0	0.0	0.0	1.9126661341804148E-4
18-19	0.0	0.0	0.0	0.0	2.2951993610164978E-4
20-21	0.0	0.0	0.0	0.0	2.677732587852581E-4
22-23	0.0	0.0	0.0	3.8253322683608296E-5	3.4427990415247464E-4
24-25	0.0	0.0	0.0	2.2951993610164978E-4	3.8253322683608296E-4
26-27	0.0	0.0	0.0	5.546731789123204E-4	4.207865495196913E-4
28-29	0.0	0.0	0.0	0.0024099593290673226	4.207865495196913E-4
30-31	0.0	0.0	0.0	0.010137130511156198	4.207865495196913E-4
32-33	0.0	0.0	0.0	0.029971478322607104	5.355465175705162E-4
34-35	0.0	0.0	0.0	0.05820243046311002	5.355465175705162E-4
36-37	0.0	0.0	0.0	0.09842579926492415	5.355465175705162E-4
38-39	0.0	0.0	0.0	0.1489584385299707	5.737998402541245E-4
40-41	1.9126661341804148E-5	0.0	0.0	0.211043581245467	5.737998402541245E-4
42-43	3.8253322683608296E-5	0.0	0.0	0.28242428137308007	6.120531629377327E-4
44-45	3.8253322683608296E-5	0.0	0.0	0.3830113933696281	6.311798242795369E-4
46-47	3.8253322683608296E-5	0.0	0.0	0.49756096814569317	7.459397923303618E-4
48-49	3.8253322683608296E-5	0.0	0.0	0.6069080910367874	9.180797444065991E-4
50-51	3.8253322683608296E-5	0.0	0.0	0.7124681349822046	9.180797444065991E-4
52-53	3.8253322683608296E-5	0.0	0.0	0.8315889818189608	0.001032839712457424
54-55	3.8253322683608296E-5	0.0	0.0	0.9885423647898056	0.0012049796645336613
56-57	3.8253322683608296E-5	0.0	0.0	1.1782405919778192	0.0012241063258754655
58-59	3.8253322683608296E-5	0.0	0.0	1.3570174955396626	0.0012241063258754655
60-61	3.8253322683608296E-5	0.0	0.0	1.5327341332868172	0.0012623596485590739
62-63	3.8253322683608296E-5	0.0	0.0	1.7237329734460736	0.0013388662939262904
64-65	3.8253322683608296E-5	0.0	0.0	1.9627971135572837	0.0013388662939262904
66-67	3.8253322683608296E-5	0.0	0.0	2.2608670039079595	0.0013388662939262904
68-69	3.8253322683608296E-5	0.0	0.0	2.5699538511915145	0.0013388662939262904
70-71	3.8253322683608296E-5	0.0	0.0	2.8592254773249604	0.0013771196166098988
72-73	3.8253322683608296E-5	0.0	0.0	3.1640662057906352	0.0014918795846607237
74-75	3.8253322683608296E-5	0.0	0.0	3.5172400074670485	0.001549259568686136
76-77	3.8253322683608296E-5	0.0	0.0	3.945772854830171	0.0015683862300279402
78-79	3.8253322683608296E-5	0.0	0.0	4.41795274337529	0.0015683862300279402
80-81	3.8253322683608296E-5	0.0	0.0	4.873224663294254	0.0015875128913697444
82-83	3.8253322683608296E-5	0.0	0.0	5.331308202430463	0.0016831461980787651
84-85	3.8253322683608296E-5	0.0	0.0	5.836137301886042	0.0016831461980787651
86-87	3.8253322683608296E-5	0.0	0.0	6.4243395181305445	0.0016831461980787651
88-89	3.8253322683608296E-5	0.0	0.0	7.072828970924414	0.0017022728594205693
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3030	0.0	56.22971	1
GTATCAA	5065	0.0	40.75903	1
TATCAAC	6250	0.0	32.121758	2
TCAACGC	6315	0.0	31.714762	4
ATCAACG	6350	0.0	31.54237	3
CAACGCA	6400	0.0	31.439453	5
AACGCAG	6730	0.0	30.171818	6
ACGCAGA	8025	0.0	24.42039	7
CGCAGAG	8070	0.0	23.815186	8
GTACATG	7515	0.0	23.808191	1
TACATGG	7590	0.0	22.886444	2
GTGGTAT	1325	0.0	21.132467	1
ACATGGG	7945	0.0	21.088047	3
GCAGAGT	8980	0.0	19.975054	9
CATGGGG	6780	0.0	18.759768	4
GAGTACT	4125	0.0	18.636824	12-13
TGGTATC	1435	0.0	18.190714	2
CAGAGTA	8555	0.0	18.138626	10-11
GTATAGG	525	0.0	18.07943	1
GAGTACA	6120	0.0	17.835712	1
>>END_MODULE