FastQCFastQC Report
Mon 15 Aug 2016
SRR2921360_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921360_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2614152
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27600.055.9648741
GTATCAA50550.039.7704471
TATCAAC65100.030.5428162
TCAACGC65800.030.2900094
ATCAACG66650.029.9037153
CAACGCA67800.029.3086665
AACGCAG71600.027.8169086
GTGGTAT15100.027.3831621
TGGTATC16250.023.9461962
GTACATG75450.023.5588251
ACGCAGA84950.022.8299337
TACATGG77250.022.2989372
CGCAGAG85800.022.2713558
ACATGGG79050.021.4309983
CATGGGG68400.019.2874
GCAGAGT94050.018.9025619
AGAGTAC80300.017.96598210-11
GAGTACT45550.017.65526612-13
ATGGGGG42950.017.2667625
CAGAGTA88700.017.12202510-11
Produced by FastQC (version 0.11.5)