##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921360_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2614152
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.45059353855476	33.0	31.0	34.0	29.0	34.0
2	31.580333890301713	34.0	31.0	34.0	29.0	34.0
3	31.647358302042115	34.0	31.0	34.0	28.0	34.0
4	35.13922794083894	37.0	35.0	37.0	33.0	37.0
5	34.88265104706995	37.0	35.0	37.0	32.0	37.0
6	34.83706762269371	37.0	35.0	37.0	32.0	37.0
7	34.71018364655154	37.0	35.0	37.0	32.0	37.0
8	34.67494124289636	37.0	35.0	37.0	32.0	37.0
9	36.29998561675067	39.0	37.0	39.0	32.0	39.0
10-11	36.157378759919084	39.0	37.0	39.0	32.0	39.0
12-13	36.09210673289082	39.0	37.0	39.0	32.0	39.0
14-15	37.33275532562759	40.0	38.0	41.0	32.0	41.0
16-17	37.30855015316631	40.0	38.0	41.0	32.0	41.0
18-19	37.27526976243156	40.0	38.0	41.0	31.0	41.0
20-21	37.240668101931334	40.0	38.0	41.0	31.0	41.0
22-23	37.131504212455894	40.0	38.0	41.0	31.0	41.0
24-25	37.05883686181982	40.0	37.5	41.0	31.0	41.0
26-27	36.92056104618247	40.0	37.0	41.0	30.0	41.0
28-29	36.767752219457776	40.0	37.0	41.0	30.0	41.0
30-31	36.620313585438026	40.0	37.0	41.0	30.0	41.0
32-33	36.56088628358259	40.0	37.0	41.0	30.0	41.0
34-35	36.68567053484266	40.0	37.0	41.0	30.0	41.0
36-37	36.653097448044335	40.0	37.0	41.0	30.0	41.0
38-39	36.55593879009331	40.0	37.0	41.0	30.0	41.0
40-41	36.418673435974654	40.0	37.0	41.0	30.0	41.0
42-43	36.30365678812862	40.0	36.0	41.0	29.0	41.0
44-45	36.152744943675806	40.0	36.0	41.0	28.5	41.0
46-47	35.983706188469526	40.0	36.0	41.0	28.0	41.0
48-49	35.814419169199034	40.0	35.5	41.0	27.5	41.0
50-51	35.65192115837181	39.5	35.0	41.0	27.0	41.0
52-53	35.468433549388095	39.0	35.0	41.0	27.0	41.0
54-55	35.25741846686803	39.0	35.0	41.0	26.0	41.0
56-57	34.987256670614414	39.0	35.0	41.0	26.0	41.0
58-59	34.764389943660504	38.5	35.0	40.5	26.0	41.0
60-61	34.48668287077415	38.0	34.0	40.0	25.5	41.0
62-63	34.18851658205031	37.0	34.0	40.0	24.5	41.0
64-65	33.86984938136727	37.0	34.0	40.0	24.0	41.0
66-67	33.53262453751733	36.0	34.0	39.0	23.0	41.0
68-69	33.170354286973364	36.0	34.0	39.0	22.0	41.0
70-71	32.787675123711246	35.0	33.0	38.5	22.0	40.0
72-73	32.398704436467355	35.0	33.0	37.5	20.5	40.0
74-75	32.000426715814534	35.0	33.0	37.0	19.5	39.0
76-77	29.256959618262442	33.0	29.0	34.5	13.0	36.5
78-79	30.914748836333924	34.5	31.5	36.0	16.5	37.0
80-81	31.117107383197304	35.0	32.0	35.5	18.0	37.0
82-83	30.964466871092426	35.0	32.0	35.0	16.0	37.0
84-85	30.742044456481487	35.0	32.0	35.0	12.5	36.5
86-87	30.498907485104155	35.0	32.0	35.0	7.5	36.0
88-89	30.224877130327542	35.0	32.0	35.0	2.0	36.0
90-91	30.04732356802512	34.5	31.5	35.0	2.0	35.0
92-93	29.9104051715432	34.0	31.0	35.0	2.0	35.0
94-95	29.81663250644951	34.0	31.0	35.0	2.0	35.0
96-97	29.629212264627306	34.0	31.0	35.0	2.0	35.0
98-99	29.366902154121107	34.0	31.0	35.0	2.0	35.0
100-101	28.28862839651252	33.5	29.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	46466.0
3	16104.0
4	8775.0
5	6177.0
6	5538.0
7	5638.0
8	5944.0
9	6402.0
10	6999.0
11	7637.0
12	8404.0
13	9053.0
14	10014.0
15	11262.0
16	12215.0
17	13277.0
18	12304.0
19	10419.0
20	9682.0
21	9570.0
22	10184.0
23	11352.0
24	13294.0
25	15814.0
26	19134.0
27	22912.0
28	27661.0
29	33372.0
30	40604.0
31	50594.0
32	63852.0
33	85044.0
34	122493.0
35	185263.0
36	322386.0
37	624667.0
38	657116.0
39	86526.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.667647141076834	19.613829975929978	14.09166988787883	25.62685299511436
2	15.600388655542549	20.91158086407002	36.981186929544705	26.506843550842724
3	18.356694720734506	24.160673297628158	29.01185921958684	28.470772762050494
4	13.899669436220623	17.1758506728528	34.235902646461255	34.68857724446532
5	14.890916597505452	36.68965153078736	33.4725358232136	14.946896048493585
6	33.799494743156	34.528536606638724	16.79141246983684	14.880556180368432
7	28.939256690898908	31.72624503279845	20.449377770501133	18.88512050580151
8	26.896140162962396	34.26884969970544	19.50089897096515	19.33411116636701
9	26.433811040827003	16.016321927722643	19.524266377777575	38.025600653672775
10-11	25.824439341042044	25.39953801338096	27.169159281996595	21.606863363580405
12-13	26.346583498572286	22.96109605306671	27.012537758543093	23.67978268981791
14-15	23.657622514378772	24.657465858482915	24.4237635708669	27.261148056271413
16-17	23.737724007104422	27.598811390484162	25.407446482542873	23.256018119868546
18-19	23.69649944618572	26.715844023099727	26.904395263687704	22.68326126702685
20-21	24.1383175282025	25.69688577079181	27.047564581715523	23.117232119290165
22-23	24.089186296404254	25.52500893217135	26.717042582931484	23.668762188492913
24-25	23.906043847377674	25.885510687564008	26.55550850326669	23.652936961791628
26-27	23.574929789156112	26.202631469721492	26.6584305325313	23.564008208591094
28-29	23.626711093981175	26.405069610550086	26.53706999693	23.431149298538738
30-31	24.017104709916623	25.836115698969653	26.509392707810864	23.63738688330286
32-33	23.772908522769118	25.86310887340904	26.3363675578685	24.02761504595334
34-35	24.080430505241484	26.120766269335878	26.306143521896168	23.49265970352647
36-37	24.045960162325297	26.04914783426325	26.400736611194493	23.504155392216962
38-39	23.90897447961973	26.058184967480774	26.488841862696265	23.543998690203225
40-41	24.03717171624432	25.889483691946214	26.484216614917077	23.58912797689239
42-43	23.94267322691108	25.933566622174226	26.129976085789696	23.993784065125002
44-45	23.932016141126205	25.967656537400952	26.214620512145633	23.88570680932721
46-47	24.032564118259167	26.02558609291792	26.005596957825794	23.936252830997123
48-49	23.732598788191805	26.161896427222164	26.19590739757734	23.909597387008688
50-51	23.88348786596537	26.129117283745174	26.260141981039865	23.727252869249593
52-53	24.133850398557826	25.909481707462856	26.101325019485582	23.855342874493736
54-55	23.626101226794383	25.905731543572813	26.3152559360093	24.1529112936235
56-57	23.693718025572274	26.099032786113863	26.06860053335823	24.138648654955638
58-59	23.70227077269625	26.074959677295944	26.22877128227729	23.99399826773051
60-61	23.731327124599076	26.00040092549519	26.414658723649094	23.85361322625664
62-63	23.73764223422781	25.97325505831697	26.416880652089446	23.872222055365775
64-65	23.532091529091453	25.814330144158603	26.30758236575475	24.34599596099519
66-67	23.524864700935733	26.111126524356703	26.091495364386663	24.2725134103209
68-69	23.593149830291605	25.94691277269712	26.27100501309857	24.188932383912704
70-71	23.462784409998143	25.834636071922652	26.28194260806671	24.420636910012494
72-73	23.43080632843003	25.971847455266474	26.208964612646145	24.388381603657354
74-75	23.430314356106997	26.09365751151425	26.14087386995452	24.335154262424236
76-77	23.448224429217486	25.79483175074736	26.213252541534853	24.543691278500305
78-79	23.299906867284157	25.85410892125673	26.282718464737993	24.563265746721118
80-81	23.460983488598178	25.99198542526237	26.079058765893276	24.46797232024618
82-83	23.349624154364058	25.95333059513372	26.258690228702157	24.43835502180006
84-85	23.34893178836051	25.96054097263491	26.1652061211659	24.52532111783868
86-87	23.23710331797826	26.140044773133823	26.03221789719407	24.590634011693847
88-89	23.05742297348505	25.97753064108091	26.119920192813673	24.845126192620366
90-91	23.04805209581907	25.852774704079085	26.217909002699848	24.881264197402
92-93	23.04337533984012	25.702600974162102	26.38609894827771	24.867924737720067
94-95	22.982578858405383	25.695280077382236	26.351736098558685	24.970404965653692
96-97	22.898727426589073	25.531769192277025	26.397041833019884	25.17246154811402
98-99	22.722684009411967	25.575270291826808	26.28617901480384	25.41586668395739
100-101	22.684205633825446	25.583594066492406	26.34999462530742	25.38220567437473
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	72.0
1	55.0
2	51.0
3	74.0
4	66.0
5	68.0
6	132.0
7	327.0
8	513.0
9	585.0
10	550.0
11	432.5
12	394.0
13	414.0
14	448.5
15	513.5
16	604.5
17	703.0
18	772.5
19	860.5
20	1002.5
21	1110.0
22	1322.5
23	1666.5
24	1954.0
25	2451.0
26	3392.0
27	4820.5
28	6784.5
29	9668.5
30	13752.0
31	19133.0
32	26448.5
33	31579.5
34	34972.0
35	39701.0
36	46086.5
37	52844.0
38	59135.5
39	65089.0
40	69594.0
41	75416.0
42	81802.5
43	87636.5
44	93287.0
45	100093.0
46	104778.0
47	107741.5
48	111566.0
49	113474.5
50	114141.0
51	114276.0
52	113627.5
53	112150.0
54	109681.5
55	103765.5
56	94393.0
57	84318.0
58	75875.0
59	66552.0
60	55005.5
61	45433.0
62	37751.0
63	28747.5
64	20364.0
65	15266.0
66	11775.5
67	9100.5
68	7062.5
69	5381.5
70	4080.0
71	2979.5
72	2010.0
73	1423.0
74	1072.0
75	750.5
76	527.0
77	311.5
78	161.5
79	96.5
80	55.0
81	32.0
82	24.0
83	19.0
84	12.5
85	7.0
86	5.0
87	5.5
88	4.0
89	2.0
90	1.5
91	1.0
92	0.5
93	0.0
94	1.5
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14731354565457555
2	4.5903987220329955E-4
3	0.005814505047908461
4	0.005087691916919904
5	0.02650955261974055
6	0.0013771196166098988
7	1.5301329073443318E-4
8	0.0019891727795476313
9	0.0
10-11	0.09963077892945782
12-13	3.2515324281067056E-4
14-15	0.005451098482414182
16-17	1.9126661341804148E-4
18-19	3.2515324281067056E-4
20-21	8.415730990393826E-4
22-23	2.2951993610164978E-4
24-25	0.0014918795846607237
26-27	0.0023525793450419104
28-29	0.005527605127781399
30-31	0.014115476070251463
32-33	0.013752069504757183
34-35	0.011208223546297231
36-37	7.076864696467535E-4
38-39	2.2951993610164978E-4
40-41	0.004647778706058408
42-43	0.008606997603811867
44-45	0.007956691118190525
46-47	0.008874770862597125
48-49	0.011074336916904601
50-51	0.00539371849838877
52-53	0.0015110062460025279
54-55	8.415730990393826E-4
56-57	0.005699745079857636
58-59	0.009180797444065992
60-61	0.007650664536721659
62-63	0.01765390841848523
64-65	0.033835063913651535
66-67	0.03670406311492216
68-69	0.026719945894500392
70-71	0.04081629530341005
72-73	0.02585924613411921
74-75	0.024902913067029003
76-77	0.23455024803454427
78-79	0.06698156801899813
80-81	0.05401369162925492
82-83	0.019604827875349253
84-85	0.03285960418521953
86-87	0.08012158436081758
88-89	0.26272382019102175
90-91	0.31602982535062996
92-93	0.05135508570274414
94-95	0.0044182587699567585
96-97	0.009027784153331558
98-99	0.0012623596485590739
100-101	0.0018744128114968066
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2614152.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.19274129573077
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.36329372252003	47.95264759843531
2	12.163898701653899	14.886846127955646
3	3.974776375420859	7.296823873485332
4	1.7537640581836058	4.2927052122472125
5	0.998114978699123	3.053869583746463
6	0.6214893946219345	2.281838384588422
7	0.42873095797662225	1.8364655817853985
8	0.29629581050806547	1.450492230354313
9	0.2246113292207304	1.2370124664984932
>10	1.122968872622468	11.678390721151523
>50	0.036321287722726525	1.4928296886769719
>100	0.014040592657584295	1.5692488176113764
>500	0.0010665410840757948	0.4709030870527943
>1k	6.27377108273903E-4	0.49992662641088437
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.8253322683608296E-5
2	0.0	0.0	0.0	0.0	3.8253322683608296E-5
3	0.0	0.0	0.0	0.0	3.8253322683608296E-5
4	0.0	0.0	0.0	0.0	3.8253322683608296E-5
5	0.0	0.0	0.0	0.0	3.8253322683608296E-5
6	0.0	0.0	0.0	0.0	3.8253322683608296E-5
7	0.0	0.0	0.0	0.0	3.8253322683608296E-5
8	0.0	0.0	0.0	0.0	3.8253322683608296E-5
9	0.0	0.0	0.0	0.0	3.8253322683608296E-5
10-11	0.0	0.0	0.0	0.0	3.8253322683608296E-5
12-13	0.0	0.0	0.0	0.0	2.677732587852581E-4
14-15	0.0	0.0	0.0	0.0	3.6340656549427883E-4
16-17	0.0	0.0	0.0	0.0	4.207865495196913E-4
18-19	0.0	0.0	0.0	1.9126661341804148E-5	4.399132108614954E-4
20-21	0.0	0.0	0.0	5.7379984025412444E-5	4.5903987220329955E-4
22-23	0.0	0.0	0.0	1.1475996805082489E-4	5.355465175705162E-4
24-25	0.0	0.0	0.0	3.2515324281067056E-4	6.120531629377327E-4
26-27	0.0	0.0	0.0	6.503064856213411E-4	6.120531629377327E-4
28-29	1.9126661341804148E-5	0.0	0.0	0.002448212651750931	6.120531629377327E-4
30-31	3.8253322683608296E-5	0.0	0.0	0.010213637156523415	6.120531629377327E-4
32-33	3.8253322683608296E-5	0.0	0.0	0.030028858306632513	6.120531629377327E-4
34-35	3.8253322683608296E-5	0.0	0.0	0.0580876704950592	6.120531629377327E-4
36-37	3.8253322683608296E-5	0.0	0.0	0.0981580260061389	6.503064856213411E-4
38-39	3.8253322683608296E-5	0.0	0.0	0.1488819318846035	6.694331469631453E-4
40-41	3.8253322683608296E-5	0.0	0.0	0.21102445458412517	7.268131309885577E-4
42-43	3.8253322683608296E-5	0.0	0.0	0.2825964213251563	7.459397923303618E-4
44-45	3.8253322683608296E-5	0.0	0.0	0.38241846686803216	8.033197763557742E-4
46-47	3.8253322683608296E-5	0.0	0.0	0.49541878207541107	8.798264217229908E-4
48-49	3.8253322683608296E-5	0.0	0.0	0.6040008385128333	9.563330670902075E-4
50-51	3.8253322683608296E-5	0.0	0.0	0.7087958160045782	9.563330670902075E-4
52-53	3.8253322683608296E-5	1.9126661341804148E-5	0.0	0.8277445228892581	0.0010710930351410324
54-55	3.8253322683608296E-5	3.8253322683608296E-5	0.0	0.9843727526172923	0.0011667263418500531
56-57	3.8253322683608296E-5	3.8253322683608296E-5	0.0	1.17347805330371	0.0012049796645336613
58-59	3.8253322683608296E-5	3.8253322683608296E-5	0.0	1.3516811570252991	0.0012241063258754655
60-61	3.8253322683608296E-5	3.8253322683608296E-5	0.0	1.5265562216734145	0.0012623596485590739
62-63	3.8253322683608296E-5	3.8253322683608296E-5	0.0	1.7166369820882643	0.0013006129712426822
64-65	3.8253322683608296E-5	3.8253322683608296E-5	0.0	1.9544961425349405	0.0013006129712426822
66-67	3.8253322683608296E-5	3.8253322683608296E-5	0.0	2.251820093093286	0.0013006129712426822
68-69	3.8253322683608296E-5	3.8253322683608296E-5	0.0	2.5604861538273216	0.0013006129712426822
70-71	3.8253322683608296E-5	3.8253322683608296E-5	0.0	2.849413500056615	0.0013006129712426822
72-73	7.650664536721659E-5	3.8253322683608296E-5	0.0	3.155325321557431	0.0014727529233189195
74-75	7.650664536721659E-5	3.8253322683608296E-5	0.0	3.5105456759974167	0.0015683862300279402
76-77	7.650664536721659E-5	3.8253322683608296E-5	0.0	3.9413928493828974	0.0015683862300279402
78-79	9.563330670902074E-5	3.8253322683608296E-5	0.0	4.4166712570653885	0.0015875128913697444
80-81	1.1475996805082489E-4	3.8253322683608296E-5	0.0	4.875864142559422	0.0016257662140533526
82-83	1.1475996805082489E-4	3.8253322683608296E-5	0.0	5.335898601152496	0.0017405261821041777
84-85	1.1475996805082489E-4	3.8253322683608296E-5	0.0	5.844151372988257	0.00179790616612959
86-87	1.1475996805082489E-4	3.8253322683608296E-5	0.0	6.439468707251912	0.00179790616612959
88-89	1.1475996805082489E-4	3.8253322683608296E-5	0.0	7.0927589520425744	0.00179790616612959
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2760	0.0	55.964874	1
GTATCAA	5055	0.0	39.770447	1
TATCAAC	6510	0.0	30.542816	2
TCAACGC	6580	0.0	30.290009	4
ATCAACG	6665	0.0	29.903715	3
CAACGCA	6780	0.0	29.308666	5
AACGCAG	7160	0.0	27.816908	6
GTGGTAT	1510	0.0	27.383162	1
TGGTATC	1625	0.0	23.946196	2
GTACATG	7545	0.0	23.558825	1
ACGCAGA	8495	0.0	22.829933	7
TACATGG	7725	0.0	22.298937	2
CGCAGAG	8580	0.0	22.271355	8
ACATGGG	7905	0.0	21.430998	3
CATGGGG	6840	0.0	19.287	4
GCAGAGT	9405	0.0	18.902561	9
AGAGTAC	8030	0.0	17.965982	10-11
GAGTACT	4555	0.0	17.655266	12-13
ATGGGGG	4295	0.0	17.266762	5
CAGAGTA	8870	0.0	17.122025	10-11
>>END_MODULE