FastQCFastQC Report
Mon 15 Aug 2016
SRR2921359_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921359_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2283190
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24410.10691182074203198No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34000.060.4342081
GTATCAA55150.037.00122
TCAACGC69100.030.2848115
ATCAACG69850.030.0974834
CAACGCA70650.029.6790986
TATCAAC69200.029.4194533
GTGGTAT16400.028.9354341
TGGTATC15750.028.6243692
AACGCAG74500.028.1508987
ACGCAGA85250.023.655928
GTACATG77900.023.3310971
CGCAGAG86250.023.1620929
TACATGG79800.021.9440272
ACATGGG81300.021.2468323
CAGAGTA87200.020.29793710-11
GAGTACT43850.020.23654412-13
GCAGAGT94050.019.2229929
CATGGGG66800.018.8972264
GTACTTT46350.018.68350814-15
AGAGTAC83350.016.9656410-11
Produced by FastQC (version 0.11.5)