##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921359_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2283190
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.529241981613445	31.0	31.0	34.0	27.0	34.0
2	30.591167620741157	33.0	31.0	34.0	27.0	34.0
3	30.61882848120393	33.0	31.0	34.0	26.0	34.0
4	34.0921053438391	37.0	35.0	37.0	30.0	37.0
5	34.08753148007831	37.0	35.0	37.0	30.0	37.0
6	34.14076358077952	37.0	35.0	37.0	30.0	37.0
7	34.0676741751672	37.0	35.0	37.0	30.0	37.0
8	34.03540879208476	37.0	35.0	37.0	30.0	37.0
9	35.60412887232337	39.0	37.0	39.0	30.0	39.0
10-11	35.50589745049689	39.0	37.0	39.0	30.0	39.0
12-13	35.4022873698641	39.0	37.0	39.0	30.0	39.0
14-15	36.53640235810423	40.0	37.0	41.0	29.5	41.0
16-17	36.47446292249002	40.0	37.0	41.0	29.0	41.0
18-19	36.44308774127427	40.0	37.0	41.0	29.0	41.0
20-21	36.39298744300737	40.0	37.0	41.0	29.0	41.0
22-23	36.27648312229819	40.0	37.0	41.0	28.0	41.0
24-25	36.17674525554159	40.0	36.0	41.0	27.0	41.0
26-27	36.02542495368323	40.0	36.0	41.0	27.0	41.0
28-29	35.881860029169715	40.0	36.0	41.0	27.0	41.0
30-31	35.722680328838166	40.0	36.0	41.0	27.0	41.0
32-33	35.519841975481675	39.5	35.0	41.0	25.5	41.0
34-35	35.35270367336928	39.0	35.0	41.0	25.0	41.0
36-37	35.17513895032827	39.0	35.0	41.0	25.0	41.0
38-39	35.39262391653783	39.5	35.0	41.0	25.5	41.0
40-41	35.49627472965456	40.0	35.0	41.0	25.0	41.0
42-43	35.307476381729074	40.0	35.0	41.0	24.0	41.0
44-45	35.15752368396848	40.0	35.0	41.0	24.0	41.0
46-47	34.96985708591926	40.0	35.0	41.0	23.0	41.0
48-49	34.80145388688633	39.5	35.0	41.0	22.0	41.0
50-51	33.07449971312068	37.0	32.5	39.5	18.0	40.0
52-53	33.226565463233456	37.5	33.5	39.5	17.5	40.0
54-55	34.08330230948804	39.0	34.0	40.5	18.5	41.0
56-57	34.096351157809906	39.0	34.0	41.0	18.0	41.0
58-59	33.918107122052916	38.0	34.0	41.0	17.5	41.0
60-61	33.675274506282875	38.0	34.0	40.0	14.5	41.0
62-63	33.38605197114563	37.0	34.0	40.0	12.5	41.0
64-65	33.09510312326175	37.0	33.0	40.0	9.5	41.0
66-67	32.76088104800739	36.0	33.0	39.5	7.5	41.0
68-69	32.39803389117857	36.0	33.0	39.0	6.0	41.0
70-71	32.017790678830934	35.0	32.5	39.0	2.0	40.5
72-73	31.665924868276402	35.0	32.0	37.5	2.0	40.0
74-75	31.27530845001949	35.0	32.0	37.0	2.0	39.0
76-77	30.902459278465656	35.0	32.0	36.5	2.0	39.0
78-79	30.56096185599972	35.0	31.5	36.0	2.0	38.5
80-81	30.21807471125925	35.0	31.0	36.0	2.0	37.0
82-83	29.921157678511207	35.0	31.0	35.0	2.0	37.0
84-85	29.689443497913008	35.0	31.0	35.0	2.0	36.5
86-87	29.464285276302014	35.0	31.0	35.0	2.0	36.0
88-89	29.259566220945256	35.0	31.0	35.0	2.0	36.0
90-91	29.005953512410265	35.0	30.0	35.0	2.0	35.5
92-93	28.820423836824794	34.0	30.0	35.0	2.0	35.0
94-95	28.616245472343522	34.0	30.0	35.0	2.0	35.0
96-97	28.364783701750618	34.0	30.0	35.0	2.0	35.0
98-99	28.128501351179708	34.0	29.5	35.0	2.0	35.0
100-101	27.093586385714723	33.5	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	72758.0
3	12773.0
4	8685.0
5	6671.0
6	6274.0
7	6362.0
8	6640.0
9	7350.0
10	7915.0
11	8573.0
12	8713.0
13	8959.0
14	9837.0
15	11559.0
16	12193.0
17	13289.0
18	11837.0
19	10073.0
20	9793.0
21	10115.0
22	10819.0
23	12356.0
24	14434.0
25	17183.0
26	20337.0
27	24125.0
28	28316.0
29	33230.0
30	40480.0
31	48794.0
32	60886.0
33	79083.0
34	112019.0
35	163369.0
36	268048.0
37	482500.0
38	519170.0
39	97656.0
40	16.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.562729866509265	18.30873463156285	13.711733100908797	24.41680240101909
2	16.929886452777765	19.791793572815276	38.0185549571185	25.259765017288455
3	19.54103828814617	23.34877549446466	29.44724600186498	27.66294021552419
4	14.354779477821571	16.52064812599314	35.101858011430814	34.02271438475447
5	15.185145909980843	36.14841119464071	33.49935059585679	15.167092299521666
6	33.40129113991701	33.93445473935611	17.180387674901183	15.483866445825697
7	28.679232885353333	31.708179285451454	20.725894061782693	18.88669376741252
8	26.825652577322295	33.37871782673111	20.039794665832574	19.755834930114013
9	26.7458940304804	15.789457544597152	19.79648777573002	37.66816064919243
10-11	25.756318930627515	25.21721683395143	27.191252229556913	21.83521200586415
12-13	26.32495392783674	23.12744831955166	26.875936037620907	23.671661714990687
14-15	23.989731684724074	24.805820435498557	24.529525897765545	26.674921982011824
16-17	23.61896525323872	27.36682861624784	25.755218892613605	23.258987237899838
18-19	23.558946541589982	26.53940393042657	27.249279675885912	22.652369852097536
20-21	24.166238602447905	25.502237069083538	27.339755209331617	22.991769119136933
22-23	24.206565520239216	25.381555792374055	26.846585431689352	23.565293255697373
24-25	23.914076993926052	25.788842023901438	26.83762990720382	23.459451074968694
26-27	23.49123843138217	26.020364798104506	27.018451080146576	23.469945690366746
28-29	23.557263181366466	26.209362021271726	26.928247079173513	23.305127718188295
30-31	24.02269667951442	25.698205212780824	26.871258304718275	23.40783980298648
32-33	23.732214959714547	25.733719148578295	26.666018384919916	23.868047506787246
34-35	24.087465587363745	25.989036139564732	26.635150495739072	23.28834777733245
36-37	23.98360950842979	25.901943496559365	26.690830618885375	23.423616376125473
38-39	23.738979243077647	26.020079092553445	26.814158342354638	23.42678332201427
40-41	23.964951762573573	25.7777425427558	26.80153406666953	23.455771628001095
42-43	23.873095197913756	25.764504937447153	26.516539246433883	23.845860618205208
44-45	23.831987413360995	25.882099209517474	26.551823020025456	23.734090357096076
46-47	23.985479914752425	25.933939492477204	26.260277179685836	23.820303413084535
48-49	23.658079301572187	26.14860899166569	26.398517662004554	23.794794044757566
50-51	23.782339254037367	26.070164237280142	26.526026633843347	23.621469874839146
52-53	24.018353829481498	25.909534630982662	26.41307569923399	23.659035840301847
54-55	23.558375654565715	25.91489998468493	26.566339052861572	23.960385307887787
56-57	23.66047025928534	26.091751711558857	26.300458866082245	23.94731916307356
58-59	23.654861431110525	26.032124284115593	26.509226954556425	23.803787330217453
60-61	23.74916969730351	25.98452998229346	26.744623138580398	23.521677181822632
62-63	23.704818437700304	25.901985477298155	26.765048524941403	23.628147560060135
64-65	23.514839807149325	25.791945361755204	26.65848310142786	24.03473172966761
66-67	23.513164702387197	25.989771733420863	26.461887770796956	24.035175793394984
68-69	23.580893720453343	25.9572864972128	26.564181935228504	23.897637847105354
70-71	23.488520913237362	25.859541196310143	26.544901392680174	24.10703649777232
72-73	23.40796122503067	26.01255114094101	26.54491275982933	24.034574874198988
74-75	23.47802354390717	26.083509194857346	26.484997818139693	23.95346944309579
76-77	23.49317262380104	25.854628662694267	26.41404789693997	24.238150816564723
78-79	23.435391944215	25.933107508485985	26.524471331977452	24.107029215321564
80-81	23.541949685396563	26.105896372897796	26.37368787213077	23.978466069574875
82-83	23.537561542570536	26.078665637244598	26.506660006733085	23.877112813451777
84-85	23.445325096069592	26.139157879352265	26.409322398593943	24.006194625984204
86-87	23.403970454574885	26.270718638274857	26.317842243689398	24.007468663460855
88-89	23.252566599906718	26.11809377486351	26.436785645696727	24.192553979533045
90-91	23.44287251288251	25.982237744003854	26.43162756152196	24.143262181591677
92-93	23.43075956640856	25.888862013426706	26.620074565506453	24.06030385465828
94-95	23.380852061929104	25.798935652480854	26.650286348249256	24.169925937340782
96-97	23.409337814678903	25.706770114304167	26.61910048058634	24.264791590430594
98-99	23.228657030915617	25.74246863991045	26.508829113576756	24.520045215597186
100-101	23.3671269310183	25.71962494550541	26.658863641252832	24.25438448222346
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	74.0
1	56.5
2	58.5
3	85.5
4	84.0
5	89.0
6	151.5
7	375.5
8	591.0
9	696.5
10	679.5
11	580.0
12	593.5
13	659.0
14	740.0
15	837.5
16	940.5
17	1068.5
18	1226.5
19	1425.0
20	1590.5
21	1762.0
22	2004.0
23	2303.5
24	2734.0
25	3377.5
26	4397.5
27	5918.5
28	7967.0
29	10674.0
30	14274.0
31	18855.0
32	24835.5
33	29131.0
34	32368.0
35	37325.0
36	43105.5
37	48763.5
38	53721.5
39	58765.0
40	62271.5
41	65494.5
42	69547.5
43	73409.0
44	77761.0
45	83580.0
46	88601.0
47	91736.5
48	94532.0
49	96119.0
50	96278.5
51	94940.5
52	93688.0
53	93403.0
54	91599.0
55	87345.0
56	81507.5
57	73831.5
58	66105.5
59	57711.0
60	47990.5
61	40007.5
62	33495.0
63	26141.0
64	19273.0
65	14573.5
66	11133.0
67	8799.0
68	6943.0
69	5335.0
70	4072.0
71	2972.0
72	2104.5
73	1535.0
74	1194.5
75	887.5
76	647.5
77	450.5
78	295.0
79	206.5
80	142.0
81	106.5
82	98.0
83	81.5
84	60.0
85	50.5
86	45.0
87	38.0
88	34.0
89	31.0
90	26.0
91	20.5
92	17.5
93	19.0
94	17.5
95	9.5
96	6.5
97	9.0
98	7.0
99	3.5
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.050762310626798465
2	0.057813848168571165
3	0.049316964422584195
4	0.056412300334181556
5	0.04809061006749329
6	0.017563146299694726
7	0.015329429438636294
8	0.020672830557246658
9	0.020891822406370036
10-11	0.022140075946373274
12-13	0.02757107380463299
14-15	0.04484953070046733
16-17	0.0301989759941135
18-19	0.02351972459585054
20-21	0.027483477064983643
22-23	0.04756502962959718
24-25	0.04309759590748032
26-27	0.03162242301341544
28-29	0.021395503659353797
30-31	0.013183309317227212
32-33	0.010161221799324629
34-35	0.010292616908798654
36-37	0.0016205396835129798
38-39	0.007380025315457759
40-41	0.004730223941064914
42-43	0.21183081565704126
44-45	0.18682194648715175
46-47	0.193391701960853
48-49	0.19085139651102187
50-51	0.18931845356715823
52-53	0.1939610807685738
54-55	0.19223104516049913
56-57	0.20315873843175558
58-59	0.1988445990040251
60-61	0.20381571397912568
62-63	0.20232656940508673
64-65	0.20320253680158026
66-67	0.19301941581734328
68-69	0.19179306146225236
70-71	0.17622274098958035
72-73	0.18369036304468747
74-75	0.18270489972363227
76-77	0.18088726737590827
78-79	0.207078692531064
80-81	0.215706971386525
82-83	0.2141302300728367
84-85	0.20863353465983997
86-87	0.22505792334409314
88-89	0.2245761412760217
90-91	0.2234154844756678
92-93	0.22744493449953795
94-95	0.23898580494833982
96-97	0.23876681309921646
98-99	0.2284084986356808
100-101	0.23830693021605737
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2283190.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.38741204494497
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.80720958564133	51.38579687747969
2	11.875529598178362	15.292692349796996
3	3.590621929301964	6.935725609787424
4	1.595802337482997	4.109983305832162
5	0.8792180636861392	2.8305287871959055
6	0.5149245083020557	1.9892793892851086
7	0.35035733904434685	1.5791021646413193
8	0.2617159924910296	1.3480972357817316
9	0.18099660901386805	1.0488512918982351
>10	0.8972818263382117	9.749570413511943
>50	0.03274306420566779	1.4176634692255803
>100	0.01202976417601625	1.4302046259636552
>500	0.0012964461140273248	0.5863446400530383
>1k	2.729360240055266E-4	0.2961598395470641
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2441	0.10691182074203198	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.759673964935025E-5
2	0.0	0.0	0.0	4.3798369824675126E-5	8.759673964935025E-5
3	0.0	0.0	0.0	4.3798369824675126E-5	8.759673964935025E-5
4	0.0	0.0	0.0	1.3139510947402538E-4	8.759673964935025E-5
5	0.0	0.0	0.0	1.3139510947402538E-4	8.759673964935025E-5
6	0.0	0.0	0.0	1.3139510947402538E-4	8.759673964935025E-5
7	0.0	0.0	0.0	1.3139510947402538E-4	8.759673964935025E-5
8	0.0	0.0	0.0	1.3139510947402538E-4	1.3139510947402538E-4
9	0.0	0.0	0.0	1.751934792987005E-4	1.751934792987005E-4
10-11	0.0	0.0	0.0	2.6279021894805077E-4	2.1899184912337562E-4
12-13	0.0	0.0	0.0	2.6279021894805077E-4	2.8468940386038835E-4
14-15	0.0	0.0	0.0	2.6279021894805077E-4	3.284877736850634E-4
16-17	0.0	0.0	0.0	3.065885887727259E-4	3.941853284220761E-4
18-19	0.0	0.0	0.0	3.50386958597401E-4	3.941853284220761E-4
20-21	0.0	0.0	0.0	3.941853284220761E-4	4.3798369824675124E-4
22-23	0.0	0.0	0.0	4.3798369824675124E-4	5.255804378961014E-4
24-25	0.0	0.0	0.0	7.226731021071396E-4	5.693788077207766E-4
26-27	0.0	0.0	0.0	0.0010949592456168781	5.912779926331142E-4
28-29	0.0	0.0	0.0	0.0027811964838668707	6.131771775454518E-4
30-31	0.0	0.0	0.0	0.00996412913511359	6.131771775454518E-4
32-33	0.0	4.3798369824675126E-5	0.0	0.02722068684603559	6.131771775454518E-4
34-35	0.0	4.3798369824675126E-5	0.0	0.054485172061895855	6.131771775454518E-4
36-37	0.0	8.759673964935025E-5	0.0	0.09118820597497361	6.350763624577893E-4
38-39	0.0	8.759673964935025E-5	0.0	0.14173152475264872	7.445722870194772E-4
40-41	0.0	8.759673964935025E-5	0.0	0.19812192590191793	7.445722870194772E-4
42-43	0.0	8.759673964935025E-5	0.0	0.26828691436104746	7.445722870194772E-4
44-45	0.0	8.759673964935025E-5	0.0	0.36488421900936846	8.321690266688274E-4
46-47	0.0	8.759673964935025E-5	0.0	0.47464293379000433	9.197657663181777E-4
48-49	0.0	1.3139510947402538E-4	0.0	0.5772625142892183	9.635641361428527E-4
50-51	0.0	1.3139510947402538E-4	0.0	0.6753927618814027	0.0010073625059675278
52-53	0.0	1.3139510947402538E-4	0.0	0.7862464359076555	0.0010511608757922028
54-55	0.0	1.3139510947402538E-4	0.0	0.9323577976427717	0.0010949592456168781
56-57	0.0	1.3139510947402538E-4	0.0	1.117581103631323	0.0011168584305292157
58-59	0.0	1.3139510947402538E-4	0.0	1.2955119810440656	0.0011825559852662285
60-61	0.0	1.3139510947402538E-4	0.0	1.4640481081294154	0.0012263543550909035
62-63	0.0	1.3139510947402538E-4	0.0	1.6382999224768855	0.0012263543550909035
64-65	0.0	1.3139510947402538E-4	0.0	1.8592188998725467	0.0012263543550909035
66-67	0.0	1.3139510947402538E-4	0.0	2.14320752981574	0.0012263543550909035
68-69	0.0	1.3139510947402538E-4	0.0	2.438605635098262	0.001248253540003241
70-71	0.0	1.3139510947402538E-4	0.0	2.7171632671831953	0.0012701527249155786
72-73	0.0	1.3139510947402538E-4	0.0	3.000056937880772	0.0013358502796525914
74-75	0.0	1.3139510947402538E-4	0.0	3.3239896811040692	0.001401547834389604
76-77	0.0	1.3139510947402538E-4	0.0	3.7309422343300382	0.001445346204214279
78-79	0.0	1.3139510947402538E-4	0.0	4.183904975056828	0.001445346204214279
80-81	0.0	1.3139510947402538E-4	0.0	4.624626071417621	0.0014672453891266166
82-83	0.0	1.3139510947402538E-4	0.0	5.0561276109303215	0.0015329429438636294
84-85	0.0	1.3139510947402538E-4	0.0	5.5278579531269845	0.0015329429438636294
86-87	0.0	1.3139510947402538E-4	0.0	6.083111786579304	0.0015329429438636294
88-89	0.0	1.3139510947402538E-4	0.0	6.710085450619528	0.0015329429438636294
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3400	0.0	60.434208	1
GTATCAA	5515	0.0	37.0012	2
TCAACGC	6910	0.0	30.284811	5
ATCAACG	6985	0.0	30.097483	4
CAACGCA	7065	0.0	29.679098	6
TATCAAC	6920	0.0	29.419453	3
GTGGTAT	1640	0.0	28.935434	1
TGGTATC	1575	0.0	28.624369	2
AACGCAG	7450	0.0	28.150898	7
ACGCAGA	8525	0.0	23.65592	8
GTACATG	7790	0.0	23.331097	1
CGCAGAG	8625	0.0	23.162092	9
TACATGG	7980	0.0	21.944027	2
ACATGGG	8130	0.0	21.246832	3
CAGAGTA	8720	0.0	20.297937	10-11
GAGTACT	4385	0.0	20.236544	12-13
GCAGAGT	9405	0.0	19.222992	9
CATGGGG	6680	0.0	18.897226	4
GTACTTT	4635	0.0	18.683508	14-15
AGAGTAC	8335	0.0	16.96564	10-11
>>END_MODULE