FastQCFastQC Report
Mon 15 Aug 2016
SRR2921359_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921359_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2283190
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23000.10073625059675279No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29900.060.7135051
GTATCAA52150.038.8193631
TCAACGC67100.029.7744264
TATCAAC67850.029.7250732
ATCAACG68000.029.450143
CAACGCA69000.029.0047445
AACGCAG74250.027.2088056
TGGTATC14150.026.1590522
GTGGTAT15350.024.7670761
ACGCAGA86900.022.8646187
CGCAGAG88750.022.1737678
GTACATG74450.021.702381
TACATGG76500.020.5949382
ACATGGG77000.020.2763123
GAGTACT47000.019.93691412-13
GCAGAGT95900.019.6779279
CAGAGTA89250.019.12025510-11
GTACTTT49550.018.4321414-15
AGAGTAC84900.018.14031410-11
CGTATAC1851.5332039E-417.956023
Produced by FastQC (version 0.11.5)