##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921359_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2283190
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.469392823199122	33.0	31.0	34.0	30.0	34.0
2	31.601260517083553	34.0	31.0	34.0	29.0	34.0
3	31.662148134846422	34.0	31.0	34.0	28.0	34.0
4	35.15923904712267	37.0	35.0	37.0	33.0	37.0
5	34.90221838743162	37.0	35.0	37.0	32.0	37.0
6	34.86433673938656	37.0	35.0	37.0	32.0	37.0
7	34.741080242993355	37.0	35.0	37.0	32.0	37.0
8	34.70598504723654	37.0	35.0	37.0	32.0	37.0
9	36.32942330686452	39.0	37.0	39.0	32.0	39.0
10-11	36.191912412020024	39.0	37.0	39.0	32.0	39.0
12-13	36.13023072981224	39.0	37.0	39.0	32.0	39.0
14-15	37.37046938712941	40.0	38.0	41.0	32.0	41.0
16-17	37.33878170454496	40.0	38.0	41.0	32.0	41.0
18-19	37.3176242450256	40.0	38.0	41.0	31.5	41.0
20-21	37.28430507316518	40.0	38.0	41.0	31.0	41.0
22-23	37.17519413627424	40.0	38.0	41.0	31.0	41.0
24-25	37.10234299379377	40.0	38.0	41.0	31.0	41.0
26-27	36.95769734450484	40.0	37.0	41.0	30.5	41.0
28-29	36.80352598776274	40.0	37.0	41.0	30.0	41.0
30-31	36.6526368808553	40.0	37.0	41.0	30.0	41.0
32-33	36.58189134500414	40.0	37.0	41.0	30.0	41.0
34-35	36.70117817614828	40.0	37.0	41.0	30.0	41.0
36-37	36.66366793827933	40.0	37.0	41.0	30.0	41.0
38-39	36.5661986080878	40.0	37.0	41.0	30.0	41.0
40-41	36.42298713641878	40.0	36.5	41.0	30.0	41.0
42-43	36.293733767229185	40.0	36.0	41.0	29.5	41.0
44-45	36.12673321098988	40.0	36.0	41.0	28.5	41.0
46-47	35.93418528462371	40.0	36.0	41.0	28.0	41.0
48-49	35.750294106053374	40.0	35.0	41.0	27.0	41.0
50-51	35.58266153933751	40.0	35.0	41.0	27.0	41.0
52-53	35.405720067099104	39.0	35.0	41.0	26.0	41.0
54-55	35.18898033015211	39.0	35.0	41.0	26.0	41.0
56-57	34.92231023261314	39.0	35.0	41.0	25.5	41.0
58-59	34.69830281316929	38.5	35.0	40.5	25.0	41.0
60-61	34.424177357118765	38.0	34.0	40.0	24.5	41.0
62-63	34.125809503370284	37.5	34.0	40.0	23.5	41.0
64-65	33.81497203474086	37.0	34.0	40.0	23.0	41.0
66-67	33.48424047056969	36.5	34.0	39.0	22.5	41.0
68-69	33.122566891060316	36.0	34.0	39.0	22.0	41.0
70-71	32.74349835099137	35.0	33.0	39.0	21.5	40.0
72-73	32.35066004143326	35.0	33.0	37.5	20.0	40.0
74-75	31.946147057406527	35.0	33.0	37.0	18.5	39.0
76-77	29.213878170454496	33.0	28.5	34.5	11.0	36.5
78-79	30.86729729019486	34.5	31.5	36.0	13.5	37.5
80-81	31.061864321409956	35.0	32.0	36.0	14.5	37.0
82-83	30.901162189743296	35.0	32.0	35.0	12.0	37.0
84-85	30.675507732602192	35.0	32.0	35.0	8.5	36.5
86-87	30.435951672878737	35.0	32.0	35.0	6.0	36.0
88-89	30.158522067808633	35.0	32.0	35.0	2.0	36.0
90-91	29.973351319863873	35.0	31.0	35.0	2.0	35.0
92-93	29.831694909315473	34.0	31.0	35.0	2.0	35.0
94-95	29.73789719646635	34.0	31.0	35.0	2.0	35.0
96-97	29.551901944209636	34.0	31.0	35.0	2.0	35.0
98-99	29.287408187667253	34.0	31.0	35.0	2.0	35.0
100-101	28.216763607058546	33.5	28.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	40561.0
3	13675.0
4	7481.0
5	5397.0
6	4924.0
7	4844.0
8	5041.0
9	5574.0
10	6036.0
11	6673.0
12	7272.0
13	8050.0
14	8807.0
15	10349.0
16	11864.0
17	13287.0
18	11795.0
19	9711.0
20	9055.0
21	8654.0
22	8938.0
23	9910.0
24	11617.0
25	13702.0
26	16635.0
27	20204.0
28	24095.0
29	28968.0
30	35152.0
31	43603.0
32	55279.0
33	73479.0
34	106705.0
35	159436.0
36	277803.0
37	537611.0
38	581172.0
39	79829.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.52838759711685	19.739591740769075	14.394348799505908	25.33767186260817
2	15.91036007251293	21.01504087064571	36.78187299375126	26.292726063090104
3	18.314527997757377	24.375394211227135	29.224060901254468	28.086016889761023
4	13.941534033023434	17.628572654970903	34.202003761542386	34.22788955046327
5	14.975297462190461	36.50459856678403	33.497535003476365	15.022568967549141
6	33.42804096240003	34.47338718543146	16.981937808316257	15.116634043852246
7	28.547264374521774	31.947858893854164	20.613405197730017	18.89147153389404
8	26.792128549447536	33.98030867179674	19.75746527694133	19.47009750181439
9	26.477472308480678	15.90975783881324	19.79116937267595	37.821600480030135
10-11	25.690442901480164	25.52590193919832	27.111554212805434	21.672100946516075
12-13	26.209751152610615	23.28180374226985	26.954215084926265	23.55423002019327
14-15	23.797352365129818	24.93845421696747	24.560517884686604	26.70367553321611
16-17	23.570382572274443	27.61450593566587	25.65682787171192	23.158283620347763
18-19	23.463681890130722	26.70529251668502	27.276051972988625	22.55497362019564
20-21	24.023756365854165	25.65939354255199	27.388518851469062	22.928331240124784
22-23	24.08942949845479	25.487520935675796	26.88125442364698	23.54179514222244
24-25	23.740778115906227	25.895295809835154	26.915747325193724	23.44817874906489
26-27	23.39078690160259	26.154790880370232	27.073928253411232	23.380493964615948
28-29	23.43852146034459	26.305624110696435	27.01662895008468	23.2392254788743
30-31	23.93642081239343	25.777737866172252	26.921716427799357	23.364124893634962
32-33	23.646284780728394	25.852306763631557	26.653968918699007	23.84743953694105
34-35	23.903134757995684	26.13697863269297	26.70180758845041	23.258079020860933
36-37	23.855114040754653	25.999145926177363	26.846409057562386	23.2993309755056
38-39	23.656346075977584	26.110788584287793	26.86497576960971	23.367889570124913
40-41	23.911875618274266	25.88947725396763	26.800225223202894	23.398421904555207
42-43	23.818369728784553	25.91578495055082	26.446052026707832	23.81979329395679
44-45	23.773936727058018	26.015682011109952	26.50017652227466	23.710204739557373
46-47	23.9100506225873	26.07428997189191	26.21439237345339	23.801267032067397
48-49	23.566559662152795	26.2645014040099	26.383843888028757	23.785095045808546
50-51	23.67804436832786	26.207777104944157	26.464320168546646	23.649858358181334
52-53	23.991103521208963	26.04950921982585	26.251423197901318	23.70796406106387
54-55	23.521901566681414	26.060630614452712	26.46587858109558	23.951589237770293
56-57	23.611838693400426	26.21470958224415	26.1981528267375	23.97529889761793
58-59	23.61456876219486	26.13992979791111	26.395275824738757	23.850225615155278
60-61	23.654398814198604	26.1235700366139	26.636663917375852	23.585367231811645
62-63	23.588726943860543	26.06315439944576	26.628388618128717	23.719730038564983
64-65	23.40674059239014	25.88358385387891	26.523354339174748	24.1863212145562
66-67	23.40391645171735	26.180475927794554	26.237523117913735	24.178084502574354
68-69	23.432081241402273	26.104035784068902	26.37699445364456	24.086888520884262
70-71	23.37094854031118	25.971471799768036	26.362360898295663	24.29521876162513
72-73	23.304437159858452	26.135575807824203	26.301089000579385	24.258898031737967
74-75	23.321292379633725	26.158396916885003	26.195919481351854	24.324391222129417
76-77	23.234815256929856	25.897659181353355	26.31923351398505	24.54829204773174
78-79	23.206607549638253	26.00317974082154	26.30550713140701	24.4847055781332
80-81	23.300154627508096	26.14992842698665	26.1646746807699	24.385242264735354
82-83	23.224935246992338	26.054814173926854	26.36094120481664	24.35930937426417
84-85	23.09951179717611	26.050591184133914	26.289851313477207	24.56004570521277
86-87	23.017127487877858	26.259094348025435	26.141291912462815	24.582486251633892
88-89	22.953700672466297	26.060544581988214	26.196148847638202	24.78960589790729
90-91	23.01022714840635	25.92028738124456	26.235862813768534	24.833622656580555
92-93	22.898476292057975	25.836448657514747	26.375493591364414	24.889581459062864
94-95	22.76286572715747	25.71743810914376	26.47709456317947	25.042601600519298
96-97	22.730933264591823	25.64234366215445	26.45131942310569	25.17540365014804
98-99	22.53816584510813	25.658752417974995	26.311706951832775	25.491374785084098
100-101	22.502240862067797	25.629352106716937	26.35720250188171	25.511204529333558
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	85.0
1	63.5
2	47.0
3	56.5
4	59.5
5	72.5
6	118.0
7	306.0
8	483.0
9	556.5
10	516.5
11	438.5
12	447.5
13	491.5
14	586.0
15	667.5
16	724.0
17	839.5
18	972.5
19	1103.5
20	1230.5
21	1405.5
22	1681.5
23	1975.0
24	2409.0
25	3073.0
26	4142.0
27	5807.5
28	7990.0
29	10923.0
30	14681.5
31	19267.0
32	25174.0
33	29599.0
34	32818.0
35	37371.0
36	43184.5
37	48825.0
38	53605.0
39	58508.5
40	62431.0
41	65654.0
42	69374.0
43	73508.5
44	77962.5
45	83668.5
46	88374.0
47	92057.0
48	95466.0
49	97016.0
50	96983.5
51	95279.0
52	94448.5
53	94506.5
54	93030.5
55	88569.0
56	81878.0
57	74111.5
58	66093.0
59	57766.5
60	48208.5
61	39977.5
62	33220.5
63	25555.5
64	18480.0
65	13848.5
66	10702.0
67	8400.0
68	6541.5
69	5001.5
70	3702.0
71	2703.0
72	1979.0
73	1405.0
74	1012.5
75	742.5
76	511.0
77	297.0
78	164.5
79	98.5
80	61.5
81	39.5
82	27.5
83	18.5
84	15.0
85	8.5
86	6.0
87	5.0
88	3.0
89	1.5
90	1.5
91	2.0
92	2.0
93	1.5
94	3.0
95	2.5
96	0.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15162995633302528
2	4.8178206807142636E-4
3	0.006569755473701269
4	0.0036790630652727106
5	0.027636771359370003
6	0.0010073625059675278
7	4.3798369824675126E-5
8	0.0023213136007077815
9	0.0
10-11	0.10069245222692812
12-13	1.5329429438636294E-4
14-15	0.00584708237159413
16-17	2.1899184912337563E-5
18-19	1.751934792987005E-4
20-21	5.474796228084391E-4
22-23	2.6279021894805077E-4
24-25	0.0014891445740389544
26-27	0.003109684257551934
28-29	0.006723049768087632
30-31	0.01609590091056811
32-33	0.016227296020042137
34-35	0.012548232954769423
36-37	6.569755473701268E-4
38-39	1.5329429438636294E-4
40-41	0.005321501933698028
42-43	0.008299791081775938
44-45	0.008409287006337625
46-47	0.009504246251954502
48-49	0.01158466881862657
50-51	0.006241267700016205
52-53	0.0017300356080746677
54-55	8.759673964935026E-4
56-57	0.0058689815565064665
58-59	0.009175758478269439
60-61	0.007752311458967497
62-63	0.018373416141451215
64-65	0.03543288118816218
66-67	0.03806078337764268
68-69	0.026717005593051825
70-71	0.04191503992221409
72-73	0.025950534121120014
74-75	0.02468038139620443
76-77	0.23732146689500214
78-79	0.06821596100193152
80-81	0.054945054945054944
82-83	0.020913721591282373
84-85	0.032279398560785565
86-87	0.08006342003950613
88-89	0.26171715888734626
90-91	0.3148664806695895
92-93	0.052163858461188074
94-95	0.005190106824224002
96-97	0.010577306312659043
98-99	0.0012920519098279161
100-101	0.001248253540003241
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2283190.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.724653966440414
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.23526646872432	50.49239937658157
2	12.13387717655215	15.464542486941497
3	3.797973072702348	7.2607355949544665
4	1.63462053254562	4.166625112116327
5	0.8706213841987939	2.7740023221925756
6	0.5432140819877829	2.076967764262143
7	0.3636477219281707	1.6221327671889916
8	0.243112343492654	1.2393799971231823
9	0.20224917844570314	1.1599433020304362
>10	0.9269984098699786	9.962432626134671
>50	0.03406656802942032	1.4395829291860545
>100	0.012768431286502038	1.4770863275861146
>500	0.001309042369333477	0.5669369192135659
>1k	2.7558786722760114E-4	0.2972324744885474
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2300	0.10073625059675279	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.3798369824675126E-5
2	0.0	0.0	0.0	0.0	4.3798369824675126E-5
3	0.0	0.0	0.0	0.0	4.3798369824675126E-5
4	0.0	0.0	0.0	4.3798369824675126E-5	8.759673964935025E-5
5	0.0	0.0	0.0	4.3798369824675126E-5	8.759673964935025E-5
6	0.0	0.0	0.0	4.3798369824675126E-5	1.3139510947402538E-4
7	0.0	0.0	0.0	4.3798369824675126E-5	1.3139510947402538E-4
8	0.0	0.0	0.0	4.3798369824675126E-5	1.3139510947402538E-4
9	0.0	0.0	0.0	8.759673964935025E-5	1.3139510947402538E-4
10-11	0.0	0.0	0.0	1.3139510947402538E-4	1.3139510947402538E-4
12-13	0.0	0.0	0.0	1.3139510947402538E-4	2.6279021894805077E-4
14-15	0.0	0.0	0.0	1.3139510947402538E-4	2.8468940386038835E-4
16-17	0.0	0.0	0.0	1.3139510947402538E-4	3.50386958597401E-4
18-19	0.0	0.0	0.0	1.3139510947402538E-4	3.722861435097386E-4
20-21	0.0	0.0	0.0	1.3139510947402538E-4	5.255804378961015E-4
22-23	0.0	0.0	0.0	2.6279021894805077E-4	5.912779926331142E-4
24-25	0.0	0.0	0.0	5.474796228084391E-4	6.131771775454518E-4
26-27	0.0	0.0	0.0	9.197657663181777E-4	6.131771775454518E-4
28-29	0.0	0.0	0.0	0.00269359974421752	6.350763624577893E-4
30-31	0.0	0.0	0.0	0.009986028320025929	7.00773917194802E-4
32-33	0.0	0.0	0.0	0.027417779510246626	7.883706568441522E-4
34-35	0.0	0.0	0.0	0.055010752499791954	8.321690266688274E-4
36-37	0.0	0.0	0.0	0.0918232823374314	8.759673964935025E-4
38-39	0.0	0.0	0.0	0.1426074921491422	8.978665814058401E-4
40-41	0.0	0.0	0.0	0.19884459900402507	9.197657663181777E-4
42-43	0.0	0.0	0.0	0.26833071273087217	9.416649512305152E-4
44-45	0.0	0.0	0.0	0.36440243694129704	9.635641361428527E-4
46-47	0.0	0.0	0.0	0.4732851843254394	9.854633210551903E-4
48-49	0.0	0.0	0.0	0.5756419746057051	0.0010073625059675278
50-51	0.0	0.0	0.0	0.672808658061747	0.001051160875792203
52-53	0.0	0.0	0.0	0.7831367516501035	0.0011168584305292157
54-55	0.0	0.0	0.0	0.9286130370227621	0.0011387576154415532
56-57	0.0	0.0	0.0	1.1135516536074528	0.0011387576154415532
58-59	0.0	0.0	0.0	1.2914168334654583	0.0011606568003538907
60-61	0.0	0.0	0.0	1.4589236988599283	0.0011825559852662285
62-63	0.0	0.0	0.0	1.6322119490712557	0.0012701527249155786
64-65	0.0	0.0	0.0	1.8531528256518293	0.0012701527249155786
66-67	0.0	0.0	0.0	2.136791068636425	0.0012701527249155786
68-69	0.0	0.0	0.0	2.4317073918508756	0.0012701527249155786
70-71	0.0	0.0	0.0	2.7112723864417765	0.0012701527249155786
72-73	0.0	0.0	0.0	2.9943850489884767	0.0013577494645649287
74-75	0.0	0.0	0.0	3.318974767759144	0.0014453462042142793
76-77	0.0	4.3798369824675126E-5	0.0	3.727241272079853	0.0015329429438636294
78-79	0.0	4.3798369824675126E-5	0.0	4.18204354433928	0.0015767413136883045
80-81	0.0	6.569755473701269E-5	0.0	4.6263342078407845	0.0016205396835129796
82-83	0.0	8.759673964935025E-5	0.0	5.061186322645071	0.0018833299024610304
84-85	0.0	8.759673964935025E-5	0.0	5.5395740170550845	0.0018833299024610304
86-87	0.0	8.759673964935025E-5	0.0	6.100915823913034	0.0019709266421103805
88-89	0.0	8.759673964935025E-5	0.0	6.732882502113272	0.0020147250119350556
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2990	0.0	60.713505	1
GTATCAA	5215	0.0	38.819363	1
TCAACGC	6710	0.0	29.774426	4
TATCAAC	6785	0.0	29.725073	2
ATCAACG	6800	0.0	29.45014	3
CAACGCA	6900	0.0	29.004744	5
AACGCAG	7425	0.0	27.208805	6
TGGTATC	1415	0.0	26.159052	2
GTGGTAT	1535	0.0	24.767076	1
ACGCAGA	8690	0.0	22.864618	7
CGCAGAG	8875	0.0	22.173767	8
GTACATG	7445	0.0	21.70238	1
TACATGG	7650	0.0	20.594938	2
ACATGGG	7700	0.0	20.276312	3
GAGTACT	4700	0.0	19.936914	12-13
GCAGAGT	9590	0.0	19.677927	9
CAGAGTA	8925	0.0	19.120255	10-11
GTACTTT	4955	0.0	18.43214	14-15
AGAGTAC	8490	0.0	18.140314	10-11
CGTATAC	185	1.5332039E-4	17.95602	3
>>END_MODULE