FastQCFastQC Report
Mon 15 Aug 2016
SRR2921358_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921358_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2650799
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30350.054.5664521
GTATCAA51250.039.9989781
TCAACGC64350.031.2692384
ATCAACG66500.030.2565653
CAACGCA66300.030.1319735
TATCAAC68100.029.9643552
AACGCAG69700.028.6545476
GTACATG77450.024.446151
ACGCAGA80600.024.134727
TACATGG79700.023.221482
CGCAGAG82400.023.0910328
ACATGGG84550.021.664523
GTGGTAT15350.021.6395721
GCAGAGT91100.019.427129
CATGGGG64350.018.7320444
CAGAGTA84700.017.98240710-11
TAAGGCG8550.017.7599965
AGAGTAC77550.017.68244210-11
GAGTACT42250.017.46377212-13
TGGTATC17650.017.2075422
Produced by FastQC (version 0.11.5)