##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921358_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2650799
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.47483909568398	31.0	31.0	34.0	26.0	34.0
2	30.533775665374854	33.0	31.0	34.0	26.0	34.0
3	30.575007761810685	33.0	31.0	34.0	26.0	34.0
4	34.042281214079225	37.0	35.0	37.0	30.0	37.0
5	34.040992923265776	37.0	35.0	37.0	30.0	37.0
6	34.08689153723085	37.0	35.0	37.0	30.0	37.0
7	34.01129282152287	37.0	35.0	37.0	30.0	37.0
8	33.979511460506814	37.0	35.0	37.0	30.0	37.0
9	35.54672195062696	39.0	37.0	39.0	30.0	39.0
10-11	35.44617717148679	39.0	37.0	39.0	30.0	39.0
12-13	35.34434730811351	39.0	36.0	39.0	29.0	39.0
14-15	36.48481929410717	40.0	37.0	41.0	29.0	41.0
16-17	36.43650631375672	40.0	37.0	41.0	29.0	41.0
18-19	36.40165021942441	40.0	37.0	41.0	28.5	41.0
20-21	36.34279004179494	40.0	37.0	41.0	28.0	41.0
22-23	36.22442931357678	40.0	37.0	41.0	27.0	41.0
24-25	36.130379557258024	40.0	36.0	41.0	27.0	41.0
26-27	35.98722517248573	40.0	36.0	41.0	27.0	41.0
28-29	35.84554864401262	40.0	36.0	41.0	27.0	41.0
30-31	35.69804915423614	40.0	36.0	41.0	26.5	41.0
32-33	35.511223597111666	40.0	35.5	41.0	25.5	41.0
34-35	35.345342291135616	39.0	35.0	41.0	25.0	41.0
36-37	35.17112764868253	39.0	35.0	41.0	25.0	41.0
38-39	35.39035513443305	39.5	35.0	41.0	25.0	41.0
40-41	35.50160894884901	40.0	35.0	41.0	25.0	41.0
42-43	35.327202854686455	40.0	35.0	41.0	24.0	41.0
44-45	35.19402527313463	40.0	35.0	41.0	24.0	41.0
46-47	35.03170044201767	40.0	35.0	41.0	23.0	41.0
48-49	34.879244144878584	40.0	35.0	41.0	22.5	41.0
50-51	33.15550896163761	37.0	32.5	39.5	18.5	40.0
52-53	33.31142138653289	37.5	33.5	39.5	18.5	40.0
54-55	34.17613425235184	39.0	34.0	40.5	19.5	41.0
56-57	34.19495631317199	39.0	34.0	41.0	19.0	41.0
58-59	34.02045553057776	38.5	34.0	41.0	18.0	41.0
60-61	33.781589437750654	38.0	34.0	40.5	16.5	41.0
62-63	33.499564470938765	37.5	34.0	40.0	13.5	41.0
64-65	33.20746744660761	37.0	33.5	40.0	12.5	41.0
66-67	32.870187818842545	36.5	33.0	39.5	8.0	41.0
68-69	32.50541798906669	36.0	33.0	39.0	7.0	41.0
70-71	32.12714487216873	35.5	33.0	39.0	3.5	41.0
72-73	31.767313742007598	35.0	32.0	38.0	2.0	40.0
74-75	31.369111162332565	35.0	32.0	37.0	2.0	39.0
76-77	30.990052999114607	35.0	32.0	37.0	2.0	39.0
78-79	30.641878543035517	35.0	31.5	36.0	2.0	39.0
80-81	30.286962157447622	35.0	31.0	36.0	2.0	37.0
82-83	29.978732450102783	35.0	31.0	35.0	2.0	37.0
84-85	29.736512840090853	35.0	31.0	35.0	2.0	36.5
86-87	29.503128490692806	35.0	31.0	35.0	2.0	36.0
88-89	29.29413508908069	35.0	31.0	35.0	2.0	36.0
90-91	29.03380282699669	35.0	30.0	35.0	2.0	36.0
92-93	28.84175563669671	34.0	30.0	35.0	2.0	35.0
94-95	28.632531738543737	34.0	30.0	35.0	2.0	35.0
96-97	28.3814374458418	34.0	30.0	35.0	2.0	35.0
98-99	28.144643558413897	34.0	30.0	35.0	2.0	35.0
100-101	27.09400316659241	33.5	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	87390.0
3	14651.0
4	10240.0
5	7839.0
6	7120.0
7	7458.0
8	7744.0
9	8217.0
10	9191.0
11	9784.0
12	9972.0
13	10612.0
14	11420.0
15	12557.0
16	13145.0
17	14054.0
18	12838.0
19	11116.0
20	11220.0
21	11660.0
22	12812.0
23	14123.0
24	16731.0
25	19471.0
26	23417.0
27	28113.0
28	32812.0
29	38962.0
30	46483.0
31	56355.0
32	70210.0
33	90975.0
34	125915.0
35	184433.0
36	308937.0
37	561099.0
38	615000.0
39	116700.0
40	23.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.35951344176017	18.64816583863849	13.673165206435861	24.319155513165477
2	16.381186263271076	19.948876618035786	38.72633929936356	24.943597819329575
3	19.225819113722913	23.642749036614315	29.51247589176867	27.618955957894105
4	13.676951896949157	16.49011279442788	36.21628538201879	33.61664992660417
5	14.695968323593918	36.548151888835164	34.092936027080896	14.662943760490016
6	33.067642346854456	34.887194838966735	17.415695149495043	14.629467664683762
7	28.679654618822408	31.672555703750938	21.100775164550324	18.547014512876334
8	26.432034713706255	33.828808602961985	20.25654183567588	19.48261484765588
9	26.42247847468032	15.881816477005387	19.97169324716791	37.72401180114638
10-11	25.344399705530655	25.455033241000365	27.76654588291938	21.434021170549595
12-13	26.042410553828454	23.154413003573676	27.389336476393183	23.413839966204687
14-15	23.438278709390588	24.78454900926228	25.04613403486882	26.731038246478313
16-17	23.294218912205007	27.550327301516266	26.03349883000132	23.121954956277406
18-19	23.243136365574497	26.82065216365937	27.357788936001292	22.57842253476484
20-21	23.94854569457256	25.968409626956472	27.285483322292787	22.797561356178182
22-23	23.8706515562881	25.86349895481285	26.81757544888698	23.44827404001207
24-25	23.59382978506599	26.107475684955677	26.897584893668974	23.401109636309357
26-27	23.304283412110703	26.27830754098571	26.971313163484155	23.44609588341943
28-29	23.426139968557795	26.352966567990794	26.97005708308941	23.250836380361996
30-31	23.78166155685026	26.01167573555323	26.79737131827227	23.409291389324245
32-33	23.515743004267264	26.141485474678333	26.610543226034526	23.732228295019876
34-35	23.918856488973844	26.22255817072644	26.534362734214202	23.324222606085517
36-37	23.867148013895665	25.96687094183939	26.630512180588582	23.53546886367636
38-39	23.576553153352666	26.194168526780455	26.78580826714862	23.44347005271826
40-41	23.88634855877739	26.014988209644795	26.71731649591511	23.38134673566271
42-43	23.747263098141712	25.972397625268016	26.496877148431334	23.783462128158934
44-45	23.752292109473963	26.05548534892506	26.521589342047648	23.670633199553322
46-47	23.869470433539366	26.143107017953344	26.276082147484086	23.711340401023207
48-49	23.64784928150634	26.212243080591186	26.337258483093535	23.802649154808943
50-51	23.677445861495233	26.213245271408773	26.433299290853057	23.676009576242933
52-53	23.927704358429384	26.104745637015903	26.34219569104883	23.625354313505888
54-55	23.585003008741985	26.078176641881086	26.49800198365301	23.838818365723927
56-57	23.607082521682557	26.19400082182594	26.378873856522194	23.820042799969304
58-59	23.6019113565135	26.048979928186156	26.56174693842373	23.787361776876608
60-61	23.607058050124156	26.075141435263372	26.64362320749906	23.67417730711341
62-63	23.585010507024904	26.02863565931912	26.66123076027464	23.725123073381337
64-65	23.50565093753869	25.885990834887156	26.612282082856474	23.99607614471768
66-67	23.42743769764931	26.067152714111387	26.48693986093653	24.018469727302776
68-69	23.508316100649708	25.962475776283416	26.59659191631852	23.932616206748357
70-71	23.471834962030655	25.778471717807687	26.627784021810356	24.121909298351298
72-73	23.481262735551603	25.909386650732525	26.523508814896157	24.085841798819718
74-75	23.431128701435693	26.10462455294023	26.473648465039673	23.990598280584408
76-77	23.51253347826013	25.829844174088272	26.563611670512504	24.09401067713909
78-79	23.416113764859166	25.850154458406998	26.516018290415825	24.217713486318008
80-81	23.497108886874187	26.009035153848654	26.43958303245139	24.054272926825764
82-83	23.536335956679533	25.975758700794298	26.517377959392924	23.970527383133245
84-85	23.538114636423384	25.948487650044623	26.469701491944853	24.043696221587144
86-87	23.449062201233737	26.052446553628055	26.43065144066664	24.06783980447157
88-89	23.35877412333064	25.935804967429092	26.541075334192765	24.164345575047495
90-91	23.354960677555958	25.901845130066548	26.585564125831823	24.157630066545675
92-93	23.417501109775934	25.74689621950196	26.66870976353265	24.166892907189457
94-95	23.32986368144247	25.74706710420206	26.735984071745218	24.187085142610258
96-97	23.428922253580144	25.623634131113583	26.695057705419682	24.25238590988659
98-99	23.32630655532217	25.62716357015781	26.56855646031887	24.477973414201152
100-101	23.433742924395855	25.52069280367783	26.718910688324378	24.32665358360194
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	83.0
1	64.5
2	65.0
3	95.0
4	99.5
5	108.0
6	174.5
7	366.0
8	583.0
9	677.5
10	623.5
11	569.5
12	603.0
13	636.5
14	708.0
15	803.0
16	900.5
17	1017.5
18	1146.0
19	1281.0
20	1418.5
21	1628.0
22	1883.5
23	2145.5
24	2522.5
25	3026.5
26	3808.0
27	5063.0
28	6901.0
29	9686.5
30	13808.0
31	19220.0
32	26224.5
33	32672.0
34	38461.0
35	45269.5
36	53738.0
37	61706.5
38	67328.5
39	72970.0
40	77929.5
41	81992.0
42	86236.5
43	90812.0
44	95986.5
45	101341.0
46	105203.0
47	108255.0
48	111958.0
49	113669.5
50	112439.5
51	109985.5
52	107956.0
53	106589.5
54	104070.0
55	99591.5
56	92502.5
57	82632.0
58	73941.5
59	65080.5
60	54100.0
61	44589.5
62	36600.5
63	28228.0
64	20268.5
65	14598.0
66	10867.5
67	8275.5
68	6155.5
69	4684.0
70	3632.0
71	2669.5
72	1974.5
73	1500.0
74	1146.5
75	876.5
76	661.0
77	455.0
78	280.0
79	204.5
80	147.0
81	113.0
82	95.0
83	67.0
84	53.5
85	49.0
86	38.5
87	37.5
88	35.0
89	28.5
90	26.5
91	25.0
92	24.5
93	21.0
94	15.5
95	12.0
96	9.0
97	7.0
98	4.0
99	2.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05024900039572974
2	0.057416650602327826
3	0.04862684798055228
4	0.05530408001511997
5	0.04738194031309051
6	0.017994574466038353
7	0.014863443059998137
8	0.02071073664959131
9	0.020673012174819743
10-11	0.022936480661113874
12-13	0.027444555396316354
14-15	0.04460919141738019
16-17	0.030028681918168824
18-19	0.023992765954717804
20-21	0.027142759598143804
22-23	0.048042118621592966
24-25	0.0415723711982689
26-27	0.03095293155007226
28-29	0.020352354139261408
30-31	0.013014943796191262
32-33	0.010279919375252517
34-35	0.009827225677993693
36-37	0.001678739127334815
38-39	0.00752603271692799
40-41	0.004357176836116205
42-43	0.21504836843532835
44-45	0.1900370416617782
46-47	0.19584661077659982
48-49	0.19333793320429046
50-51	0.19182895421342772
52-53	0.19579002406444243
54-55	0.19522415694286893
56-57	0.20635287700048174
58-59	0.20175049107835036
60-61	0.2077675448044156
62-63	0.2046930001105327
64-65	0.20527772946949205
66-67	0.19512984575594
68-69	0.19420559612403657
70-71	0.1779463474974904
72-73	0.18517058441624581
74-75	0.18484992638068748
76-77	0.18354843200106835
78-79	0.21082322726091265
80-81	0.21868878025078478
82-83	0.21757590824502349
84-85	0.21229448177700383
86-87	0.2289309751512657
88-89	0.2274974451099461
90-91	0.22630912415464166
92-93	0.23091151007677305
94-95	0.2430022042410609
96-97	0.24219112803347217
98-99	0.23215641774423484
100-101	0.24194591894745698
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2650799.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.559531957827204
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.32706935404268	46.82891127751268
2	12.780633227915455	15.479783328144286
3	4.2466705245659755	7.715291380404481
4	1.9050424835943904	4.61473924665012
5	1.0235540169058706	3.099297609868674
6	0.6335329973304377	2.301987707890242
7	0.4045695172261745	1.7150378425334865
8	0.29094133420017154	1.4095416821078537
9	0.2153141192290632	1.1735390055981485
>10	1.109858877471326	11.639398535023982
>50	0.047808512494278525	1.9686446827093715
>100	0.01375429697562562	1.4118206182132282
>500	0.0010631273413325296	0.4555250825024194
>1k	1.8761070727976723E-4	0.18648200084113176
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	7.544894954313775E-5	0.0
5	0.0	0.0	0.0	7.544894954313775E-5	0.0
6	0.0	0.0	0.0	7.544894954313775E-5	0.0
7	0.0	0.0	0.0	7.544894954313775E-5	0.0
8	0.0	0.0	0.0	7.544894954313775E-5	0.0
9	0.0	0.0	0.0	1.1317342431470662E-4	0.0
10-11	0.0	0.0	0.0	1.1317342431470662E-4	0.0
12-13	0.0	0.0	0.0	1.1317342431470662E-4	0.0
14-15	0.0	0.0	0.0	1.1317342431470662E-4	5.6586712157353315E-5
16-17	0.0	0.0	0.0	1.3203566170049105E-4	7.544894954313775E-5
18-19	0.0	3.7724474771568874E-5	0.0	1.8862237385784437E-4	1.1317342431470662E-4
20-21	0.0	3.7724474771568874E-5	0.0	2.074846112436288E-4	1.1317342431470662E-4
22-23	0.0	3.7724474771568874E-5	0.0	3.7724474771568873E-4	1.1317342431470662E-4
24-25	0.0	3.7724474771568874E-5	0.0	6.224538337308864E-4	1.1317342431470662E-4
26-27	0.0	3.7724474771568874E-5	0.0	0.0012449076674617729	1.1317342431470662E-4
28-29	0.0	3.7724474771568874E-5	0.0	0.0029425090321823725	1.1317342431470662E-4
30-31	0.0	3.7724474771568874E-5	0.0	0.010393092799567225	1.1317342431470662E-4
32-33	0.0	3.7724474771568874E-5	0.0	0.029783472832153622	1.1317342431470662E-4
34-35	1.8862237385784437E-5	3.7724474771568874E-5	0.0	0.05790706877435822	1.1317342431470662E-4
36-37	3.7724474771568874E-5	3.7724474771568874E-5	0.0	0.10112045462519037	1.1317342431470662E-4
38-39	3.7724474771568874E-5	3.7724474771568874E-5	0.0	0.16466733237789816	1.1317342431470662E-4
40-41	3.7724474771568874E-5	3.7724474771568874E-5	0.0	0.23615521207002116	1.3203566170049105E-4
42-43	3.7724474771568874E-5	3.7724474771568874E-5	0.0	0.3133017629778795	1.508978990862755E-4
44-45	3.7724474771568874E-5	3.7724474771568874E-5	0.0	0.4191000524747444	1.508978990862755E-4
46-47	3.7724474771568874E-5	3.7724474771568874E-5	0.0	0.544005788443409	1.508978990862755E-4
48-49	3.7724474771568874E-5	3.7724474771568874E-5	0.0	0.6660256020920485	1.508978990862755E-4
50-51	3.7724474771568874E-5	3.7724474771568874E-5	0.0	0.7889319408978198	1.508978990862755E-4
52-53	3.7724474771568874E-5	3.7724474771568874E-5	0.0	0.92034514876458	1.8862237385784437E-4
54-55	3.7724474771568874E-5	3.7724474771568874E-5	0.0	1.0806175798315905	2.2634684862941323E-4
56-57	3.7724474771568874E-5	3.7724474771568874E-5	0.0	1.2738046151367945	2.2634684862941323E-4
58-59	3.7724474771568874E-5	3.7724474771568874E-5	0.0	1.4752344481795867	2.4520908601519765E-4
60-61	3.7724474771568874E-5	3.7724474771568874E-5	0.0	1.6769283525457797	2.640713234009821E-4
62-63	3.7724474771568874E-5	3.7724474771568874E-5	0.0	1.8887512783881388	2.640713234009821E-4
64-65	3.7724474771568874E-5	3.7724474771568874E-5	0.0	2.1383552657142246	2.640713234009821E-4
66-67	3.7724474771568874E-5	3.7724474771568874E-5	0.0	2.448186377013119	2.640713234009821E-4
68-69	3.7724474771568874E-5	3.7724474771568874E-5	0.0	2.772692308998155	2.640713234009821E-4
70-71	3.7724474771568874E-5	3.7724474771568874E-5	0.0	3.0993108115703984	3.01795798172551E-4
72-73	3.7724474771568874E-5	3.7724474771568874E-5	0.0	3.436963723013325	3.7724474771568873E-4
74-75	3.7724474771568874E-5	3.7724474771568874E-5	0.0	3.815472240633862	3.7724474771568873E-4
76-77	5.6586712157353315E-5	3.7724474771568874E-5	0.0	4.262167746404009	3.7724474771568873E-4
78-79	7.544894954313775E-5	3.7724474771568874E-5	0.0	4.755905672214302	3.7724474771568873E-4
80-81	7.544894954313775E-5	3.7724474771568874E-5	0.0	5.254453468557971	4.149692224872576E-4
82-83	7.544894954313775E-5	3.7724474771568874E-5	0.0	5.762187174508517	4.5269369725882647E-4
84-85	7.544894954313775E-5	7.544894954313775E-5	0.0	6.311512113894716	4.5269369725882647E-4
86-87	7.544894954313775E-5	7.544894954313775E-5	0.0	6.9331737336554	4.5269369725882647E-4
88-89	7.544894954313775E-5	7.544894954313775E-5	0.0	7.608196622980468	4.904181720303954E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3035	0.0	54.566452	1
GTATCAA	5125	0.0	39.998978	1
TCAACGC	6435	0.0	31.269238	4
ATCAACG	6650	0.0	30.256565	3
CAACGCA	6630	0.0	30.131973	5
TATCAAC	6810	0.0	29.964355	2
AACGCAG	6970	0.0	28.654547	6
GTACATG	7745	0.0	24.44615	1
ACGCAGA	8060	0.0	24.13472	7
TACATGG	7970	0.0	23.22148	2
CGCAGAG	8240	0.0	23.091032	8
ACATGGG	8455	0.0	21.66452	3
GTGGTAT	1535	0.0	21.639572	1
GCAGAGT	9110	0.0	19.42712	9
CATGGGG	6435	0.0	18.732044	4
CAGAGTA	8470	0.0	17.982407	10-11
TAAGGCG	855	0.0	17.759996	5
AGAGTAC	7755	0.0	17.682442	10-11
GAGTACT	4225	0.0	17.463772	12-13
TGGTATC	1765	0.0	17.207542	2
>>END_MODULE