FastQCFastQC Report
Mon 15 Aug 2016
SRR2921358_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921358_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2650799
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27950.056.1364821
GTATCAA47900.041.490991
TCAACGC62100.031.4923484
ATCAACG64450.030.5650123
TATCAAC65500.029.785722
CAACGCA66050.029.6621825
AACGCAG70100.028.0798876
GTACATG77050.025.3619251
TACATGG80600.023.6165772
ACGCAGA81150.023.611387
ACATGGG81600.022.8031753
CGCAGAG83000.022.684128
CATGGGG59650.019.973894
GCAGAGT90700.019.5002639
AGAGTAC76200.019.0304810-11
CAGAGTA83300.018.20750810-11
GAGTACT40250.017.91863812-13
GTGGTAT14950.017.8098341
TGGTATC14050.016.2170732
ATGGGGG36550.016.1329235
Produced by FastQC (version 0.11.5)