FastQCFastQC Report
Mon 15 Aug 2016
SRR2921357_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921357_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1939637
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19500.10053427522778748No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25400.058.657861
GTATCAA42950.040.3236661
TCAACGC53700.031.3702454
TATCAAC54400.031.0554182
ATCAACG55600.030.2990253
CAACGCA56350.029.7234945
AACGCAG59050.028.5162936
GTGGTAT11500.028.469611
GTACATG65700.025.9274221
TGGTATC11450.025.6964132
TACATGG68400.024.2827722
ACGCAGA69250.023.7044897
CGCAGAG71000.023.188248
ACATGGG71050.022.7753663
CATGGGG63000.021.6174874
GCAGAGT77600.020.2989239
CAGAGTA71700.020.01769610-11
GAGTACT37200.019.4511812-13
GTACTTT37850.018.92788714-15
ATGGGGG38050.018.5802125
Produced by FastQC (version 0.11.5)