##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921357_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1939637
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.43536960781837	31.0	31.0	34.0	26.0	34.0
2	30.491509493786722	31.0	31.0	34.0	26.0	34.0
3	30.522280199851828	33.0	31.0	34.0	26.0	34.0
4	33.99652048295635	37.0	35.0	37.0	30.0	37.0
5	33.993797292998636	37.0	35.0	37.0	30.0	37.0
6	34.038985129691795	37.0	35.0	37.0	30.0	37.0
7	33.96092413168031	37.0	35.0	37.0	30.0	37.0
8	33.930137958803634	37.0	35.0	37.0	30.0	37.0
9	35.48110084515814	39.0	37.0	39.0	30.0	39.0
10-11	35.38510762580833	39.0	36.0	39.0	30.0	39.0
12-13	35.26997319601554	39.0	35.0	39.0	29.0	39.0
14-15	36.38683294863936	40.0	37.0	41.0	28.5	41.0
16-17	36.32570862486125	40.0	37.0	41.0	28.0	41.0
18-19	36.2814562724881	40.0	37.0	41.0	27.5	41.0
20-21	36.22360214823702	40.0	36.5	41.0	27.0	41.0
22-23	36.10804083444479	40.0	36.0	41.0	27.0	41.0
24-25	36.00065733949187	40.0	36.0	41.0	27.0	41.0
26-27	35.83819369294358	40.0	36.0	41.0	27.0	41.0
28-29	35.69449180439433	40.0	36.0	41.0	26.5	41.0
30-31	35.52939776875776	40.0	35.5	41.0	25.5	41.0
32-33	35.33200207049051	39.0	35.0	41.0	25.0	41.0
34-35	35.15680511353413	39.0	35.0	41.0	25.0	41.0
36-37	34.9702552591026	39.0	35.0	41.0	24.0	41.0
38-39	35.188182118612914	39.0	35.0	41.0	24.5	41.0
40-41	35.2889739162534	40.0	35.0	41.0	24.5	41.0
42-43	35.09850064728606	40.0	35.0	41.0	23.5	41.0
44-45	34.94307955560757	40.0	35.0	41.0	23.0	41.0
46-47	34.75439270337697	39.5	35.0	41.0	21.5	41.0
48-49	34.5765011700643	39.0	35.0	41.0	20.0	41.0
50-51	32.85122190389232	37.0	32.5	39.5	16.0	40.0
52-53	32.99860850251877	37.5	32.5	39.5	14.5	40.0
54-55	33.84549377022608	38.0	34.0	40.0	15.0	41.0
56-57	33.84447424956319	38.0	34.0	41.0	14.0	41.0
58-59	33.65799992472819	38.0	34.0	40.5	13.0	41.0
60-61	33.40288337456957	37.5	33.5	40.0	10.5	41.0
62-63	33.109828024522116	37.0	33.0	40.0	8.0	41.0
64-65	32.813555062106985	36.5	33.0	40.0	7.0	41.0
66-67	32.467546762615896	36.0	33.0	39.0	2.0	41.0
68-69	32.09865015979794	35.5	32.5	39.0	2.0	41.0
70-71	31.721772682208062	35.0	32.0	38.5	2.0	40.5
72-73	31.36928172642613	35.0	32.0	37.0	2.0	40.0
74-75	30.980329309040815	35.0	32.0	37.0	2.0	39.0
76-77	30.614379649388006	35.0	31.0	36.5	2.0	39.0
78-79	30.279880462168954	35.0	31.0	36.0	2.0	38.0
80-81	29.94293184755704	35.0	31.0	35.5	2.0	37.0
82-83	29.65294614404654	35.0	31.0	35.0	2.0	37.0
84-85	29.429430609954338	35.0	31.0	35.0	2.0	36.0
86-87	29.209803690071904	35.0	30.0	35.0	2.0	36.0
88-89	29.00615450210529	35.0	30.0	35.0	2.0	36.0
90-91	28.760409293079064	34.0	30.0	35.0	2.0	35.0
92-93	28.570253093748985	34.0	30.0	35.0	2.0	35.0
94-95	28.369067253305644	34.0	30.0	35.0	2.0	35.0
96-97	28.112579828081234	34.0	29.0	35.0	2.0	35.0
98-99	27.875248564550994	34.0	29.0	35.0	2.0	35.0
100-101	26.826259501133457	33.0	26.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	64321.0
3	11321.0
4	8012.0
5	6133.0
6	5518.0
7	5781.0
8	6029.0
9	6714.0
10	7261.0
11	7797.0
12	7840.0
13	8012.0
14	8926.0
15	9834.0
16	10440.0
17	11414.0
18	10112.0
19	8668.0
20	8610.0
21	8930.0
22	9826.0
23	10991.0
24	12720.0
25	15086.0
26	17826.0
27	21212.0
28	24811.0
29	29727.0
30	35219.0
31	42958.0
32	54019.0
33	69561.0
34	97625.0
35	142549.0
36	233458.0
37	410886.0
38	416422.0
39	73063.0
40	5.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	44.006887206518705	18.27755875557793	13.675736988353338	24.039817049550024
2	16.92056271707893	19.506397396368268	37.931868747494875	25.641171139057935
3	19.618370774461905	23.355279446718605	29.279131041198998	27.747218737620493
4	14.542964437887527	16.205263646586676	34.80784904414572	34.44392287138008
5	15.43083154005022	36.218816990214144	33.1514259953495	15.19892547438614
6	33.94696798205968	33.6476361031888	17.005093024922665	15.40030288982885
7	29.155380790988417	31.614064560107373	20.3658299520824	18.864724696821806
8	27.176392693310454	33.292888436025216	19.793746493515066	19.73697237714927
9	26.966194216951468	15.684845822156355	19.626361477145334	37.72259848374684
10-11	26.02193293779823	25.007509425779674	26.975146572388674	21.99541106403342
12-13	26.49804976923924	22.810518843635766	26.863136268103805	23.82829511902119
14-15	24.20647364832315	24.485897048401757	24.369511464225035	26.938117839050058
16-17	23.97432383335452	27.132866736167177	25.438218077747916	23.45459135273039
18-19	23.867775541749765	26.340028955201944	26.939038083954614	22.853157419093673
20-21	24.391602318668085	25.403189728592274	27.085386981433174	23.11982097130647
22-23	24.4751356422912	25.12462997855823	26.675251284468928	23.724983094681644
24-25	24.258096760367458	25.484557447245272	26.55656939671379	23.700776395673476
26-27	23.833509363280427	25.77702437877244	26.642080921516776	23.74738533643036
28-29	23.831617388470463	25.90469982911025	26.656996321280985	23.60668646113831
30-31	24.33236739387127	25.454575923357343	26.525402317223456	23.68765436554793
32-33	24.058876470422938	25.517150347482442	26.330576624667657	24.093396557426964
34-35	24.402636712632198	25.777065323062782	26.257894914590942	23.562403049714085
36-37	24.277587476258137	25.68219829532917	26.40886424707622	23.631349981336474
38-39	24.10657409367138	25.725071918771427	26.441462541269996	23.7268914462872
40-41	24.279452950347707	25.488691296206166	26.502241736304168	23.729614017141962
42-43	24.145963655565414	25.61321093484797	26.15986033056271	24.080965079023905
44-45	24.093312960317043	25.68583300531951	26.261923431758145	23.958930602605307
46-47	24.22398751922677	25.65212169270487	25.979922795879173	24.143967992189186
48-49	23.953871363009892	25.882604850491063	26.087119357124998	24.076404429374048
50-51	24.034200299509617	25.87800794594156	26.2298465103617	23.85794524418712
52-53	24.30668542592285	25.68059344271526	26.088702834629686	23.924018296732203
54-55	23.80387332338213	25.629003716590653	26.302298376442153	24.264824583585064
56-57	23.871124400214406	25.82014504640064	26.04803451148553	24.260696041899426
58-59	23.926621723759727	25.724604924156907	26.25300869324425	24.095764658839116
60-61	23.996417562211533	25.667231621342424	26.47746497319759	23.858885843248455
62-63	23.96859494568126	25.668625736976214	26.3946493408803	23.968129976462222
64-65	23.804413978666837	25.574143744142575	26.303073293868806	24.31836898332178
66-67	23.828975964516317	25.638107557588402	26.151496851629048	24.381419626266233
68-69	23.791368350307845	25.616252138333902	26.318651075824423	24.273728435533833
70-71	23.74696779881757	25.520616643772627	26.317763062579363	24.41465249483044
72-73	23.683085004997558	25.732833319128385	26.211695063651085	24.37238661222297
74-75	23.72022845619543	25.862483089706302	26.196375838024828	24.220912616073445
76-77	23.824172259574272	25.567423903355795	26.09125941337551	24.517144423694422
78-79	23.67932738844552	25.66173452293089	26.186611680027983	24.472326408595606
80-81	23.8715492847128	25.804334790526635	26.05131962498608	24.272796299774484
82-83	23.82774202432108	25.731405841532972	26.25760211263876	24.183250021507188
84-85	23.74791008347266	25.80010994665934	26.195928253604784	24.25605171626322
86-87	23.680130261683466	26.006785410050686	26.00892991174213	24.304154416523723
88-89	23.563081929889247	25.82097700533198	26.117740740731172	24.498200324047602
90-91	23.705110573071135	25.65052899848001	26.18698143641516	24.457378992033696
92-93	23.73756556339302	25.562566209337778	26.28217450843604	24.417693718833167
94-95	23.760758500271457	25.50160251813573	26.309463914206425	24.428175067386384
96-97	23.67339270883454	25.374473427025553	26.360975690430134	24.591158173709776
98-99	23.49343127742889	25.43259001068411	26.210604782396935	24.86337392949007
100-101	23.721740667391582	25.447818429136674	26.299629548470854	24.53081135500089
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	92.0
1	73.0
2	75.0
3	103.0
4	103.0
5	118.5
6	173.0
7	360.5
8	575.0
9	699.0
10	641.0
11	511.0
12	496.0
13	537.5
14	637.0
15	752.0
16	841.0
17	951.0
18	1093.0
19	1169.5
20	1310.0
21	1497.0
22	1658.5
23	1907.0
24	2215.0
25	2602.0
26	3255.5
27	4297.5
28	5712.5
29	7799.5
30	10576.0
31	14139.5
32	18750.5
33	22054.0
34	24515.0
35	27874.0
36	32495.0
37	37651.5
38	41707.0
39	45741.5
40	49862.0
41	53358.5
42	56925.0
43	61050.0
44	65146.5
45	69896.5
46	73857.0
47	77242.5
48	80311.5
49	82362.5
50	83411.5
51	83156.0
52	82847.0
53	82568.5
54	81012.0
55	76986.5
56	72118.0
57	65911.5
58	59182.5
59	51641.0
60	43329.5
61	36836.5
62	30749.0
63	23917.0
64	17753.5
65	13451.5
66	10325.0
67	8102.0
68	6463.5
69	5135.0
70	4014.5
71	3073.0
72	2325.5
73	1805.0
74	1516.5
75	1201.5
76	871.0
77	585.5
78	392.5
79	288.0
80	214.5
81	161.0
82	107.5
83	74.5
84	56.5
85	45.0
86	41.5
87	33.5
88	32.5
89	37.5
90	26.0
91	23.5
92	24.5
93	14.0
94	8.5
95	10.5
96	10.0
97	6.0
98	5.0
99	5.0
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05000935742100197
2	0.057433426976284736
3	0.04892668061085657
4	0.054494732777318644
5	0.047483111530662696
6	0.01639482026791611
7	0.013713906261841777
8	0.0192304023897255
9	0.019745962775509027
10-11	0.021009085720678663
12-13	0.02616468957851392
14-15	0.04243061974998415
16-17	0.029000271700323307
18-19	0.02240109876229418
20-21	0.026113133539935565
22-23	0.04629732264336059
24-25	0.041554167094152154
26-27	0.029541610105396007
28-29	0.019333514466882206
30-31	0.012734341528853077
32-33	0.00961520119486275
34-35	0.009099640809079225
36-37	0.0012889009644588138
38-39	0.006315614725848188
40-41	0.003557366661906326
42-43	0.2170509224148642
44-45	0.19297425239877358
46-47	0.19898053089315165
48-49	0.19761429587082532
50-51	0.1962222828292098
52-53	0.2001147637418754
54-55	0.19887741881599494
56-57	0.20877617822303865
58-59	0.2060179301590968
60-61	0.21052908353470265
62-63	0.207513055277869
64-65	0.20926596058953298
66-67	0.19972809345253778
68-69	0.19810407823731965
70-71	0.18268882270239226
72-73	0.1903448944312776
74-75	0.19003555819980747
76-77	0.18707108598155223
78-79	0.21326155357935533
80-81	0.22145896371331333
82-83	0.21846871347576893
84-85	0.21411222821589812
86-87	0.2297079298858498
88-89	0.2282643608056559
90-91	0.2280581366513425
92-93	0.2313319451010679
94-95	0.24300938783906476
96-97	0.24334450208982403
98-99	0.23300751635486433
100-101	0.24236493735683534
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1939637.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.87108701196995
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.36980851872231	53.59917737084091
2	10.727289767410152	14.13236475343378
3	3.280297392500579	6.482302648996313
4	1.4762101420285298	3.8895826685405517
5	0.8466232212383242	2.7883995936271964
6	0.5460516294918493	2.1581408639571347
7	0.3595073441033365	1.6576797681411168
8	0.2698400383064365	1.4219725314077265
9	0.19765864064921418	1.171799056518488
>10	0.8909506876028015	9.782411534090777
>50	0.025083564341904535	1.0895292550172373
>100	0.009658800236337696	1.2503812273466506
>500	7.848102832754997E-4	0.32734101248907477
>1k	2.3544308498264893E-4	0.24891771559300216
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1950	0.10053427522778748	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.5466811573505764E-4
2	0.0	0.0	0.0	0.0	1.5466811573505764E-4
3	0.0	0.0	0.0	0.0	1.5466811573505764E-4
4	0.0	0.0	0.0	0.0	1.5466811573505764E-4
5	0.0	0.0	0.0	0.0	1.5466811573505764E-4
6	0.0	0.0	0.0	0.0	2.062241543134102E-4
7	0.0	0.0	0.0	0.0	2.062241543134102E-4
8	0.0	0.0	0.0	0.0	2.062241543134102E-4
9	0.0	0.0	0.0	0.0	2.577801928917627E-4
10-11	0.0	0.0	0.0	2.5778019289176274E-5	2.577801928917627E-4
12-13	0.0	0.0	0.0	5.155603857835255E-5	3.608922700484678E-4
14-15	0.0	0.0	0.0	5.155603857835255E-5	4.124483086268204E-4
16-17	0.0	0.0	0.0	5.155603857835255E-5	4.6400434720517294E-4
18-19	0.0	0.0	0.0	7.733405786752882E-5	4.6400434720517294E-4
20-21	0.0	0.0	0.0	1.804461350242339E-4	5.155603857835254E-4
22-23	0.0	0.0	0.0	2.577801928917627E-4	5.155603857835254E-4
24-25	0.0	0.0	0.0	5.155603857835254E-4	5.67116424361878E-4
26-27	0.0	0.0	0.0	8.248966172536408E-4	5.928944436510543E-4
28-29	0.0	0.0	0.0	0.0031964743918578577	6.186724629402305E-4
30-31	0.0	5.155603857835255E-5	0.0	0.012734341528853079	6.186724629402305E-4
32-33	0.0	5.155603857835255E-5	0.0	0.03286697459369975	6.186724629402305E-4
34-35	0.0	5.155603857835255E-5	0.0	0.06588861730313456	6.186724629402305E-4
36-37	0.0	5.155603857835255E-5	0.0	0.11048459067340952	6.702285015185831E-4
38-39	0.0	7.733405786752882E-5	0.0	0.16920691861415305	6.960065208077594E-4
40-41	0.0	1.2889009644588136E-4	0.0	0.2334715207020695	8.248966172536408E-4
42-43	0.0	1.5466811573505764E-4	0.0	0.30755754813916214	8.248966172536408E-4
44-45	0.0	1.5466811573505764E-4	0.0	0.41046340114155383	8.248966172536408E-4
46-47	0.0	1.5466811573505764E-4	0.0	0.5339916695752864	8.50674636542817E-4
48-49	0.0	1.5466811573505764E-4	0.0	0.6510754331867251	9.280086944103459E-4
50-51	0.0	1.5466811573505764E-4	0.0	0.7582862154104093	9.280086944103459E-4
52-53	0.0	1.5466811573505764E-4	0.0	0.8739006319223649	9.537867136995221E-4
54-55	0.0	1.5466811573505764E-4	0.0	1.030656767219846	9.795647329886984E-4
56-57	0.0	1.5466811573505764E-4	0.0	1.2181660795293139	9.795647329886984E-4
58-59	0.0	1.5466811573505764E-4	0.0	1.4001588957108986	9.795647329886984E-4
60-61	5.155603857835255E-5	1.5466811573505764E-4	0.0	1.5702422669808835	9.795647329886984E-4
62-63	5.155603857835255E-5	1.5466811573505764E-4	0.0	1.7565400123837605	9.795647329886984E-4
64-65	5.155603857835255E-5	1.5466811573505764E-4	0.0	1.9869955048289962	0.0010053427522778748
66-67	5.155603857835255E-5	2.062241543134102E-4	0.0	2.2705537170099355	0.0010568987908562272
68-69	5.155603857835255E-5	2.062241543134102E-4	0.0	2.576281025779566	0.0011084548294345796
70-71	5.155603857835255E-5	2.062241543134102E-4	0.0	2.86406683312393	0.001134232848723756
72-73	5.155603857835255E-5	2.062241543134102E-4	0.0	3.151723750371848	0.0015466811573505764
74-75	5.155603857835255E-5	2.062241543134102E-4	0.0	3.4852655419545	0.0015466811573505764
76-77	5.155603857835255E-5	2.062241543134102E-4	0.0	3.897559182465585	0.0015466811573505764
78-79	5.155603857835255E-5	2.062241543134102E-4	0.0	4.358315499240321	0.0015724591766397527
80-81	5.155603857835255E-5	2.062241543134102E-4	0.0	4.804739237290276	0.001701349273085634
82-83	5.155603857835255E-5	2.062241543134102E-4	0.0	5.243738905784948	0.001804461350242339
84-85	5.155603857835255E-5	2.062241543134102E-4	0.0	5.719704253940299	0.001804461350242339
86-87	5.155603857835255E-5	2.577801928917627E-4	0.0	6.2803246174413045	0.001804461350242339
88-89	5.155603857835255E-5	2.577801928917627E-4	0.0	6.906782042206866	0.001804461350242339
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2540	0.0	58.65786	1
GTATCAA	4295	0.0	40.323666	1
TCAACGC	5370	0.0	31.370245	4
TATCAAC	5440	0.0	31.055418	2
ATCAACG	5560	0.0	30.299025	3
CAACGCA	5635	0.0	29.723494	5
AACGCAG	5905	0.0	28.516293	6
GTGGTAT	1150	0.0	28.46961	1
GTACATG	6570	0.0	25.927422	1
TGGTATC	1145	0.0	25.696413	2
TACATGG	6840	0.0	24.282772	2
ACGCAGA	6925	0.0	23.704489	7
CGCAGAG	7100	0.0	23.18824	8
ACATGGG	7105	0.0	22.775366	3
CATGGGG	6300	0.0	21.617487	4
GCAGAGT	7760	0.0	20.298923	9
CAGAGTA	7170	0.0	20.017696	10-11
GAGTACT	3720	0.0	19.45118	12-13
GTACTTT	3785	0.0	18.927887	14-15
ATGGGGG	3805	0.0	18.580212	5
>>END_MODULE