FastQCFastQC Report
Mon 15 Aug 2016
SRR2921357_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921357_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1939637
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19610.10110139165214935No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23850.056.8010181
GTATCAA41900.041.407451
TCAACGC56400.030.2078724
TATCAAC57100.029.9214322
ATCAACG56800.029.9115893
CAACGCA57500.029.6896975
GTGGTAT10750.028.2990061
AACGCAG60900.027.9518936
TGGTATC11750.023.8303342
GTACATG63750.023.2634471
ACGCAGA72700.023.0219737
CGCAGAG73750.022.1139538
TACATGG68000.021.0074622
ACATGGG70200.019.469743
GCAGAGT80100.019.1736289
GAGTACT35300.019.15332412-13
AGAGTAC67450.019.03338610-11
CGAATAG1300.007516331518.2896675
CAGAGTA73450.018.22304210-11
AGTACTT38550.017.90781612-13
Produced by FastQC (version 0.11.5)