FastQCFastQC Report
Mon 15 Aug 2016
SRR2921356_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921356_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2971853
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32050.053.304281
GTATCAA58100.043.943411
TCAACGC73200.034.5573434
CAACGCA75250.033.29725
TATCAAC76950.032.9960562
ATCAACG77200.032.7042243
AACGCAG81600.030.8140686
GTACATG121750.027.4014761
ACGCAGA94050.026.183227
TACATGG126500.025.8491082
GTGGTAT17200.025.107331
CGCAGAG97200.024.7030128
ACATGGG130450.024.3747563
CATGGGG112350.022.3886014
TGGTATC17750.021.3898872
GCAGAGT106000.020.8170939
GAGTACA93850.020.4789831
AGTACAT89800.020.1356432
ATGGGGG76150.020.128575
GTATATA9400.019.6890321
Produced by FastQC (version 0.11.5)