##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921356_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2971853
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.477124204999374	31.0	31.0	34.0	26.0	34.0
2	30.526192580857803	31.0	31.0	34.0	26.0	34.0
3	30.569499568114573	33.0	31.0	34.0	26.0	34.0
4	34.043082884651426	37.0	35.0	37.0	30.0	37.0
5	34.036083547874	37.0	35.0	37.0	30.0	37.0
6	34.08484504448908	37.0	35.0	37.0	30.0	37.0
7	34.005564541718584	37.0	35.0	37.0	30.0	37.0
8	33.97362621906265	37.0	35.0	37.0	30.0	37.0
9	35.53338640908551	39.0	37.0	39.0	30.0	39.0
10-11	35.4242871366787	39.0	37.0	39.0	30.0	39.0
12-13	35.30729649144827	39.0	35.0	39.0	29.0	39.0
14-15	36.429985265085456	40.0	37.0	41.0	29.0	41.0
16-17	36.371435262780494	40.0	37.0	41.0	29.0	41.0
18-19	36.32594546230921	40.0	37.0	41.0	28.0	41.0
20-21	36.26371728345917	40.0	37.0	41.0	27.5	41.0
22-23	36.15283444369557	40.0	36.0	41.0	27.0	41.0
24-25	36.04562338715946	40.0	36.0	41.0	27.0	41.0
26-27	35.89344745517359	40.0	36.0	41.0	27.0	41.0
28-29	35.74766248532481	40.0	36.0	41.0	27.0	41.0
30-31	35.596380608327536	40.0	35.5	41.0	26.0	41.0
32-33	35.412592581126994	39.0	35.0	41.0	25.0	41.0
34-35	35.254524197529285	39.0	35.0	41.0	25.0	41.0
36-37	35.08276671154327	39.0	35.0	41.0	24.5	41.0
38-39	35.324577460594455	39.0	35.0	41.0	25.0	41.0
40-41	35.4504596963578	40.0	35.0	41.0	25.0	41.0
42-43	35.286924857992645	40.0	35.0	41.0	24.0	41.0
44-45	35.16481350187913	40.0	35.0	41.0	24.0	41.0
46-47	35.00876220997472	40.0	35.0	41.0	23.0	41.0
48-49	34.86263200097717	39.0	35.0	41.0	23.0	41.0
50-51	33.14232736275987	37.0	32.5	39.5	18.5	40.0
52-53	33.298846544563276	37.5	33.5	39.5	19.5	40.0
54-55	34.16089120155001	39.0	34.0	40.5	20.5	41.0
56-57	34.177651956540245	39.0	34.0	41.0	20.0	41.0
58-59	33.99992933701633	38.0	34.0	41.0	19.5	41.0
60-61	33.74942754570969	38.0	34.0	40.0	18.0	41.0
62-63	33.45655320098269	37.0	34.0	40.0	17.0	41.0
64-65	33.168655381003035	37.0	33.0	40.0	14.0	41.0
66-67	32.83160455782974	36.0	33.0	39.0	12.5	41.0
68-69	32.46978400344835	36.0	33.0	39.0	9.0	41.0
70-71	32.089978373762094	35.0	32.5	39.0	7.0	40.5
72-73	31.731867626023224	35.0	32.0	37.5	4.0	40.0
74-75	31.334109560600744	35.0	32.0	37.0	2.0	39.0
76-77	30.965642311379465	35.0	32.0	36.5	2.0	39.0
78-79	30.626936628426776	35.0	31.5	36.0	2.0	38.0
80-81	30.28224932390667	35.0	31.0	36.0	2.0	37.0
82-83	29.98593285064907	35.0	31.0	35.0	2.0	37.0
84-85	29.756472645181304	35.0	31.0	35.0	2.0	36.0
86-87	29.53834644580334	35.0	31.0	35.0	2.0	36.0
88-89	29.343845742033672	35.0	31.0	35.0	2.0	36.0
90-91	29.095912381938135	34.5	30.0	35.0	2.0	35.5
92-93	28.90248306359702	34.0	30.0	35.0	2.0	35.0
94-95	28.695725697065097	34.0	30.0	35.0	2.0	35.0
96-97	28.440557456913247	34.0	30.0	35.0	2.0	35.0
98-99	28.210881224609697	34.0	30.0	35.0	2.0	35.0
100-101	27.15474116653818	33.0	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	96824.0
3	17237.0
4	11930.0
5	9105.0
6	8348.0
7	8444.0
8	9060.0
9	9403.0
10	10470.0
11	11056.0
12	11262.0
13	11320.0
14	12015.0
15	12850.0
16	13144.0
17	13637.0
18	13039.0
19	11968.0
20	12220.0
21	12727.0
22	14145.0
23	16014.0
24	19000.0
25	22642.0
26	26549.0
27	31737.0
28	37597.0
29	44544.0
30	53583.0
31	64937.0
32	81541.0
33	105702.0
34	153078.0
35	218204.0
36	352508.0
37	633273.0
38	668272.0
39	112459.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.90460842044098	18.074466892474902	13.45109880361585	24.569825883468273
2	16.29694846547896	19.67890456526533	39.164451567179384	24.85969540207633
3	19.533648911283006	23.94988861855702	28.946618040595567	27.569844429564412
4	14.002237551473614	15.83540697950415	35.96895978097179	34.193395688050444
5	14.882892927791266	37.23177410515061	33.31757811290164	14.567754854156483
6	33.5184118206123	34.046218691020115	17.57435672380788	14.861012764559703
7	29.307475551358554	31.860587130855716	20.4654118063574	18.366525511428325
8	27.286581312766366	33.80437171454179	19.961652335374122	18.947394637317725
9	27.740752981373024	15.66583043952072	19.185826185464723	37.407590393641534
10-11	26.202528599499974	25.093752645141382	27.085254873412683	21.618463881945964
12-13	26.775143622185425	22.526144984822842	27.010058475438107	23.688652917553622
14-15	24.217841100051707	24.051142116942433	24.557332062219924	27.173684720785936
16-17	24.147823754270853	26.944649481270854	25.40280059548753	23.504726168970763
18-19	24.153223770786987	26.54315918430538	26.57178388602623	22.731833158881397
20-21	24.777515436176103	25.522154215350856	26.572100279189055	23.128230069283987
22-23	24.781590792299042	25.143413756577775	26.168808860824903	23.906186590298276
24-25	24.46720934150729	25.403989119573104	26.06000594818214	24.068795590737466
26-27	24.014857000528444	25.808366908000362	26.20574286685583	23.971033224615365
28-29	24.037022932941497	26.208383655132018	25.940015686770764	23.81457772515573
30-31	24.8541986089218	25.58111908162455	25.8748083464692	23.68987396298445
32-33	24.42602232897694	25.5622752585762	25.79085509048211	24.220847321964747
34-35	24.621400520256696	25.901598796611935	25.923506112847917	23.553494570283448
36-37	24.56725555993933	25.80144978619986	25.996936218324308	23.634358435536505
38-39	24.495874048264987	25.895840666451747	25.879351665961114	23.72893361932215
40-41	24.615598164050933	25.572943306323527	25.906954128193394	23.90450440143215
42-43	24.311237577997638	25.67354604341279	25.638375555060612	24.376840823528955
44-45	24.151070547426016	25.681726738224718	25.921452228771138	24.245750485578125
46-47	24.269463402497095	25.545421306489324	25.704079515205784	24.48103577580779
48-49	24.10008220951948	25.7480484720947	25.90081961727819	24.25104970110763
50-51	24.275771207435405	25.66030109146541	26.001524151017374	24.06240355008181
52-53	24.45552221958876	25.490518017099227	25.97512760025879	24.078832163053224
54-55	23.786560869054384	25.386167634217433	26.330871850922733	24.496399645805454
56-57	24.00885487324014	25.528482888990904	25.881251571021817	24.58141066674714
58-59	24.14393367623807	25.56844206490667	26.12683249517732	24.160791763677935
60-61	24.43262626569154	25.530486180654606	26.220795948438347	23.816091605215508
62-63	24.29817050524913	25.38134212399821	26.286235741342306	24.03425162941036
64-65	23.92220178759614	25.226795970701588	26.33330034646329	24.517701895238982
66-67	24.015411355794406	25.476358123200832	25.99182873139681	24.516401789607954
68-69	23.931166639358796	25.474583790992988	26.18416696503042	24.410082604617795
70-71	23.872822237990178	25.29407898174194	26.174992879137477	24.658105901130405
72-73	23.841568945256007	25.751173297429297	25.935755687146756	24.47150207016794
74-75	23.821841802489256	25.65920124216609	26.07099238108016	24.447964574264493
76-77	23.767131172560934	25.211028336393355	26.154029743277018	24.867810747768697
78-79	23.712634372617575	25.347612912449456	26.262717370816667	24.677035344116298
80-81	23.973816127152027	25.594509776547852	25.866572786718685	24.565101309581436
82-83	23.9956338099173	25.576354457211387	26.119801471211822	24.308210261659486
84-85	23.971069430937952	25.63803844820633	25.888809610837683	24.502082510018035
86-87	23.5781190743544	25.922484693338117	25.923732530490646	24.575663701816833
88-89	23.578977791432436	25.520376823607933	26.11972789758017	24.780917487379465
90-91	23.836703832879518	25.290701791896637	26.2062438158421	24.66635055938174
92-93	24.060833757710455	25.221609170592856	26.144317449825135	24.573239621871554
94-95	23.955509406769043	25.334604467441636	26.034987443531303	24.674898682258014
96-97	23.67192637144119	25.22339396247302	26.24040251304189	24.8642771530439
98-99	23.41637068285628	25.126188340640816	26.201780270320356	25.255660706182546
100-101	23.717937366822916	25.261429724239644	26.211880892572104	24.80875201636534
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	51.0
1	49.0
2	61.0
3	78.0
4	94.5
5	118.0
6	168.5
7	347.5
8	532.0
9	600.0
10	564.5
11	497.0
12	521.5
13	579.5
14	669.0
15	805.0
16	893.5
17	978.5
18	1073.0
19	1199.0
20	1320.0
21	1432.0
22	1554.0
23	1683.5
24	1893.0
25	2112.5
26	2482.0
27	3171.0
28	4331.5
29	6363.0
30	9573.0
31	14175.0
32	20947.0
33	27986.0
34	34511.5
35	41715.5
36	50915.5
37	60330.5
38	67404.5
39	73761.0
40	79322.0
41	84458.0
42	89988.5
43	95953.5
44	103079.0
45	111012.0
46	117379.0
47	123002.0
48	129004.5
49	133462.5
50	134752.5
51	132983.0
52	131299.5
53	130384.0
54	127809.0
55	121908.0
56	112749.5
57	101439.0
58	90054.0
59	77506.5
60	64010.5
61	53399.0
62	44497.5
63	34951.0
64	25920.0
65	19201.0
66	14610.5
67	11345.0
68	8876.0
69	6968.0
70	5490.0
71	4236.5
72	3149.0
73	2490.5
74	2061.0
75	1558.0
76	1104.0
77	744.0
78	506.5
79	370.0
80	270.0
81	196.0
82	136.5
83	101.5
84	90.0
85	83.0
86	74.0
87	60.0
88	50.0
89	43.5
90	39.5
91	31.0
92	26.0
93	23.0
94	16.0
95	16.5
96	15.5
97	11.5
98	8.0
99	7.0
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05000247320442834
2	0.057472560049235275
3	0.048118126973305884
4	0.05525172341969808
5	0.04741149713663495
6	0.016858168960577794
7	0.014132596733418509
8	0.02072780854234715
9	0.01921360174948088
10-11	0.020307195544328742
12-13	0.02612847943690351
14-15	0.04135466996516988
16-17	0.028332491546519963
18-19	0.02163633261806691
20-21	0.02516948180142154
22-23	0.0450728888676526
24-25	0.04029472521016349
26-27	0.029039121383190893
28-29	0.018826637791303943
30-31	0.011608918745308063
32-33	0.00819354120139859
34-35	0.008176716681477852
36-37	0.0011945409143722789
38-39	0.005888581972257712
40-41	0.0031293607052569556
42-43	0.21193847744151545
44-45	0.1865334523612036
46-47	0.19275852473187605
48-49	0.19016754866408264
50-51	0.18882158707042374
52-53	0.19361657524783357
54-55	0.1913452650585342
56-57	0.20285323668431782
58-59	0.19911819326191438
60-61	0.20468710935567808
62-63	0.20120443373208569
64-65	0.2027859386046349
66-67	0.19275852473187605
68-69	0.19095830110035725
70-71	0.17558068989280426
72-73	0.18261333921967204
74-75	0.1824450940204647
76-77	0.18061122134910443
78-79	0.20613401806886142
80-81	0.21412566503121117
82-83	0.21328443903517436
84-85	0.20865769605697185
86-87	0.22620567033429986
88-89	0.22363151878642717
90-91	0.22258839855134155
92-93	0.22622249485422058
94-95	0.23710795924293698
96-97	0.23821837755770556
98-99	0.22684500209128783
100-101	0.23724255540230288
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2971853.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.96609043642192
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.91568107545052	45.94383096710424
2	12.38443071789503	14.605229234300001
3	3.974881290753636	7.031496289938613
4	1.821139502579543	4.295419064257831
5	0.9960268457205351	2.9365904530930576
6	0.5990837196267702	2.1195374874300104
7	0.42522090765274406	1.7551530147276384
8	0.3157383912972708	1.4894286828388197
9	0.2250642783382882	1.1944044531453186
>10	1.253065976689372	13.123888835610655
>50	0.06702237469926248	2.6605526558068138
>100	0.021213668936679666	2.1436845621207374
>500	0.0011450002884101863	0.46825853951595237
>1k	2.8625007189922013E-4	0.23252576011012638
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.3649039841472646E-5	0.0
3	0.0	0.0	0.0	3.3649039841472646E-5	0.0
4	0.0	0.0	0.0	3.3649039841472646E-5	0.0
5	0.0	0.0	0.0	3.3649039841472646E-5	0.0
6	0.0	0.0	0.0	3.3649039841472646E-5	0.0
7	0.0	0.0	0.0	1.0094711952441793E-4	6.729807968294529E-5
8	0.0	0.0	0.0	1.0094711952441793E-4	1.0094711952441793E-4
9	0.0	0.0	0.0	1.0094711952441793E-4	1.0094711952441793E-4
10-11	0.0	0.0	0.0	1.6824519920736322E-4	1.0094711952441793E-4
12-13	0.0	0.0	0.0	1.6824519920736322E-4	3.028413585732538E-4
14-15	0.0	0.0	0.0	1.6824519920736322E-4	4.542620378598807E-4
16-17	0.0	0.0	0.0	1.6824519920736322E-4	5.552091573842986E-4
18-19	0.0	0.0	0.0	1.6824519920736322E-4	5.72033677305035E-4
20-21	0.0	0.0	0.0	1.6824519920736322E-4	5.72033677305035E-4
22-23	0.0	0.0	0.0	2.5236779881104485E-4	6.056827171465076E-4
24-25	0.0	0.0	0.0	4.879110777013533E-4	6.561562769087166E-4
26-27	0.0	0.0	0.0	9.085240757197614E-4	6.729807968294529E-4
28-29	0.0	0.0	0.0	0.00260780058771413	7.066298366709255E-4
30-31	0.0	0.0	0.0	0.009791870593868539	7.066298366709255E-4
32-33	1.6824519920736323E-5	0.0	0.0	0.02821471990707481	7.402788765123982E-4
34-35	3.3649039841472646E-5	0.0	0.0	0.05570598545755796	7.402788765123982E-4
36-37	3.3649039841472646E-5	0.0	0.0	0.09596706162787998	7.402788765123982E-4
38-39	3.3649039841472646E-5	0.0	0.0	0.16072463880279408	7.402788765123982E-4
40-41	3.3649039841472646E-5	0.0	0.0	0.23529091109149747	7.739279163538708E-4
42-43	3.3649039841472646E-5	0.0	0.0	0.31625050095008067	7.907524362746072E-4
44-45	3.3649039841472646E-5	0.0	0.0	0.42261511588897566	8.075769561953435E-4
46-47	3.3649039841472646E-5	0.0	0.0	0.545097620911936	8.075769561953435E-4
48-49	3.3649039841472646E-5	0.0	0.0	0.6630038565164562	9.085240757197614E-4
50-51	3.3649039841472646E-5	0.0	0.0	0.7862771139756912	9.085240757197614E-4
52-53	3.3649039841472646E-5	0.0	0.0	0.9189384535506971	0.0010767692749271247
54-55	3.3649039841472646E-5	0.0	0.0	1.0838355732938338	0.00114406735461007
56-57	3.3649039841472646E-5	0.0	0.0	1.2790336534142166	0.0011777163944515425
58-59	3.3649039841472646E-5	0.0	0.0	1.4786902313135946	0.0011777163944515425
60-61	3.3649039841472646E-5	0.0	0.0	1.683612883948163	0.0011777163944515425
62-63	3.3649039841472646E-5	0.0	0.0	1.9022138712782901	0.0012113654342930152
64-65	3.3649039841472646E-5	0.0	0.0	2.156247297561488	0.0012618389940552241
66-67	3.3649039841472646E-5	0.0	0.0	2.458668043136723	0.0012786635139759605
68-69	3.3649039841472646E-5	0.0	0.0	2.7830111381686775	0.0012786635139759605
70-71	3.3649039841472646E-5	0.0	0.0	3.1122165194577254	0.0012786635139759605
72-73	5.047355976220897E-5	0.0	0.0	3.451331542980087	0.0013627861135796422
74-75	6.729807968294529E-5	0.0	0.0	3.8297822940771296	0.0013796106335003783
76-77	6.729807968294529E-5	0.0	0.0	4.275379704177832	0.001413259673341851
78-79	6.729807968294529E-5	0.0	0.0	4.763459027078392	0.0014300841932625872
80-81	6.729807968294529E-5	0.0	0.0	5.254381693845557	0.0015142067928662689
82-83	6.729807968294529E-5	0.0	0.0	5.764417015242678	0.001615153912390687
84-85	6.729807968294529E-5	0.0	0.0	6.310406335710414	0.001615153912390687
86-87	6.729807968294529E-5	3.3649039841472646E-5	0.0	6.916307771615891	0.0016319784323114233
88-89	6.729807968294529E-5	3.3649039841472646E-5	0.0	7.5790256112936945	0.0016488029522321594
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3205	0.0	53.30428	1
GTATCAA	5810	0.0	43.94341	1
TCAACGC	7320	0.0	34.557343	4
CAACGCA	7525	0.0	33.2972	5
TATCAAC	7695	0.0	32.996056	2
ATCAACG	7720	0.0	32.704224	3
AACGCAG	8160	0.0	30.814068	6
GTACATG	12175	0.0	27.401476	1
ACGCAGA	9405	0.0	26.18322	7
TACATGG	12650	0.0	25.849108	2
GTGGTAT	1720	0.0	25.10733	1
CGCAGAG	9720	0.0	24.703012	8
ACATGGG	13045	0.0	24.374756	3
CATGGGG	11235	0.0	22.388601	4
TGGTATC	1775	0.0	21.389887	2
GCAGAGT	10600	0.0	20.817093	9
GAGTACA	9385	0.0	20.478983	1
AGTACAT	8980	0.0	20.135643	2
ATGGGGG	7615	0.0	20.12857	5
GTATATA	940	0.0	19.689032	1
>>END_MODULE