FastQCFastQC Report
Mon 15 Aug 2016
SRR2921356_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921356_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2971853
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31750.054.93441
GTATCAA59200.044.795521
TCAACGC77250.033.7278674
ATCAACG79500.032.9523933
TATCAAC79850.032.867392
CAACGCA79500.032.8302155
AACGCAG84600.031.1299656
GTACATG122350.025.4424761
ACGCAGA104350.024.5998927
TACATGG128650.023.793862
CGCAGAG108000.023.416388
ACATGGG131200.022.3185673
GTATAGG6700.021.9891781
GTAAGTA22350.020.3849072
GCAGAGT117750.020.1855669
CATGGGG107200.020.1433474
AGAGTAC103700.017.94671210-11
TGTAAGT24050.017.784821
CAGAGTA108750.017.50673510-11
TAAGTAC31100.017.2438033
Produced by FastQC (version 0.11.5)