##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921356_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2971853
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.431958108291358	33.0	31.0	34.0	29.0	34.0
2	31.559987657532186	34.0	31.0	34.0	29.0	34.0
3	31.631775865091576	34.0	31.0	34.0	28.0	34.0
4	35.12469627535413	37.0	35.0	37.0	33.0	37.0
5	34.86481430945609	37.0	35.0	37.0	32.0	37.0
6	34.82059004937324	37.0	35.0	37.0	32.0	37.0
7	34.692537618785316	37.0	35.0	37.0	32.0	37.0
8	34.65194779149574	37.0	35.0	37.0	32.0	37.0
9	36.2728610735457	39.0	37.0	39.0	32.0	39.0
10-11	36.122621980293104	39.0	37.0	39.0	32.0	39.0
12-13	36.05195462225083	39.0	37.0	39.0	31.5	39.0
14-15	37.272733880175096	40.0	38.0	41.0	31.0	41.0
16-17	37.24699926274954	40.0	38.0	41.0	31.0	41.0
18-19	37.21549282551997	40.0	38.0	41.0	31.0	41.0
20-21	37.174643395888026	40.0	38.0	41.0	31.0	41.0
22-23	37.068724462481825	40.0	38.0	41.0	31.0	41.0
24-25	36.99141074609007	40.0	37.0	41.0	31.0	41.0
26-27	36.84894390806006	40.0	37.0	41.0	30.0	41.0
28-29	36.69752693016781	40.0	37.0	41.0	30.0	41.0
30-31	36.55540819145496	40.0	37.0	41.0	30.0	41.0
32-33	36.50352995252457	40.0	36.5	41.0	30.0	41.0
34-35	36.637441017439286	40.0	37.0	41.0	30.0	41.0
36-37	36.613208156661855	40.0	37.0	41.0	30.0	41.0
38-39	36.5287184460335	40.0	37.0	41.0	30.0	41.0
40-41	36.40626218726162	40.0	36.5	41.0	30.0	41.0
42-43	36.302479126659364	40.0	36.0	41.0	29.5	41.0
44-45	36.16484832863537	40.0	36.0	41.0	29.0	41.0
46-47	36.00659319286654	40.0	36.0	41.0	28.0	41.0
48-49	35.84987211682409	40.0	35.0	41.0	28.0	41.0
50-51	35.69310258616426	39.5	35.0	41.0	27.5	41.0
52-53	35.51765262279123	39.0	35.0	41.0	27.0	41.0
54-55	35.303681238607695	39.0	35.0	41.0	26.5	41.0
56-57	35.04367611722383	39.0	35.0	41.0	26.0	41.0
58-59	34.817915118951035	38.5	35.0	40.5	26.0	41.0
60-61	34.53688809641662	38.0	34.5	40.0	26.0	41.0
62-63	34.231915575905	37.0	34.0	40.0	25.0	41.0
64-65	33.919023753866696	37.0	34.0	40.0	25.0	41.0
66-67	33.58519768642662	36.0	34.0	39.0	23.5	41.0
68-69	33.22196454535268	36.0	34.0	39.0	23.0	41.0
70-71	32.84445394842881	35.0	33.0	38.5	22.5	40.0
72-73	32.45412693696492	35.0	33.0	37.5	21.5	39.5
74-75	32.04734049766257	35.0	33.0	37.0	20.5	39.0
76-77	29.29756956350129	33.0	29.0	34.5	13.5	36.5
78-79	30.965784983308396	34.5	31.5	36.0	18.0	37.0
80-81	31.168970504261146	35.0	32.0	35.5	18.5	37.0
82-83	31.01374344558765	35.0	32.0	35.0	18.0	37.0
84-85	30.79377210110998	35.0	32.0	35.0	16.5	36.0
86-87	30.5612160157316	35.0	32.0	35.0	12.0	36.0
88-89	30.29314437827174	35.0	32.0	35.0	7.0	36.0
90-91	30.116914766645593	34.5	32.0	35.0	3.5	35.0
92-93	29.981389725534875	34.0	31.0	35.0	2.0	35.0
94-95	29.88938500659353	34.0	31.0	35.0	2.0	35.0
96-97	29.708916288928155	34.0	31.0	35.0	2.0	35.0
98-99	29.452190939457637	34.0	31.0	35.0	2.0	35.0
100-101	28.3576998929624	33.5	29.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	53657.0
3	19169.0
4	10453.0
5	7374.0
6	6595.0
7	6481.0
8	6588.0
9	7043.0
10	7836.0
11	8511.0
12	9045.0
13	9879.0
14	10573.0
15	11443.0
16	12744.0
17	13510.0
18	12691.0
19	11233.0
20	10784.0
21	10624.0
22	11195.0
23	12722.0
24	14725.0
25	18073.0
26	21421.0
27	26124.0
28	31645.0
29	37749.0
30	46636.0
31	57709.0
32	74310.0
33	98521.0
34	146045.0
35	216008.0
36	367578.0
37	709896.0
38	744855.0
39	90406.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.952025981194836	19.53708612016606	13.98548280181453	25.52540509682457
2	15.399785991170454	20.907350328416065	37.72020027996123	25.972663400452245
3	18.20551467507675	24.91069839157834	28.850872118750697	28.03291481459421
4	13.625665857934605	16.93370528278141	35.00072348430039	34.439905374983596
5	14.69405655601749	37.526892650194945	33.30659775326885	14.47245304051871
6	33.63821924731696	34.611225032993225	17.242250713535334	14.508305006154472
7	29.1621478749425	32.012124438354704	20.460629056186903	18.365098630515885
8	27.311509986196924	34.22650634194338	19.68290619630783	18.77907747555187
9	27.5792577896686	15.594008182773509	19.073083359102892	37.753650668455
10-11	26.114640992610127	25.369826359246705	27.037874308299198	21.47765833984397
12-13	26.65475824542082	22.682783246039122	27.039569506702314	23.622889001837745
14-15	24.07891077302763	24.23676731605683	24.521033601974366	27.16328830894117
16-17	24.130458044344067	27.14425213637826	25.29210741315516	23.433182406122512
18-19	24.08858462622998	26.71878351766031	26.56719431262876	22.62543754348095
20-21	24.65069170168288	25.616844797200383	26.6159741237534	23.11648937736333
22-23	24.630478054637077	25.2735799106717	26.196676039627214	23.899265995064013
24-25	24.386477211049282	25.569492427686395	26.033838602899976	24.01019175836434
26-27	23.88969526905626	25.936108107398738	26.27844438974482	23.895752233800184
28-29	23.961147223312608	26.35805779189849	25.93333163956611	23.747463345222794
30-31	24.753554827740782	25.71774674468913	25.85508700796782	23.67361141960227
32-33	24.363581905712223	25.715201577688894	25.755417836836408	24.16579867976247
34-35	24.50008135598468	26.001978456396813	25.97897667127656	23.518963516341955
36-37	24.487346050711363	25.929096428171245	26.062565973459535	23.520991547657864
38-39	24.43956569769574	26.022672095838047	25.87342147829279	23.66434072817342
40-41	24.610663971039052	25.728431894461334	25.85766655472274	23.80323757977687
42-43	24.27871401436153	25.76293835735026	25.603362072432223	24.35498555585599
44-45	24.115563130915856	25.756198844234085	25.888719041533708	24.23951898331635
46-47	24.179738687460752	25.664218165616397	25.746833202428043	24.40920994449481
48-49	23.997839515001402	25.914765165039853	25.911719621544947	24.175675698413794
50-51	24.242988147695232	25.739185358686044	25.959766133902125	24.0580603597166
52-53	24.47373623424683	25.609133789114257	25.81168626322736	24.10544371341155
54-55	23.7246498875609	25.504899168223226	26.24385437799121	24.52659656622466
56-57	23.96471577173439	25.649561731751835	25.775449440319605	24.610273056194174
58-59	24.096876539587075	25.676324616970923	26.03118681803864	24.195612025403353
60-61	24.409365750044756	25.646849075727985	26.09276506274658	23.85102011148068
62-63	24.16539184022452	25.584020836534073	26.121778556631153	24.128808766610256
64-65	23.83207015455707	25.35700677121272	26.20480528555829	24.60611778867192
66-67	23.94671924143151	25.683685973945668	25.777887663853633	24.591707120769193
68-69	23.8071677222779	25.66718079856211	25.975121401602646	24.55053007755734
70-71	23.774206340389807	25.42905386981646	25.987643598646205	24.809096191147532
72-73	23.76212380301821	25.829361274814953	25.701965943879074	24.706548978287763
74-75	23.66546419719512	25.758387106762864	25.80242791578091	24.7737207802611
76-77	23.494435315006097	25.273124237714384	26.0114619423847	25.22097850489482
78-79	23.504786571974012	25.37479984046163	26.109575383142104	25.01083820442226
80-81	23.704735169726913	25.653734611523237	25.73310524866324	24.908424970086614
82-83	23.69184153373471	25.607747831978184	25.92069619019829	24.779714444088814
84-85	23.58584195130358	25.628539465891453	25.758299194967947	25.02731938783702
86-87	23.201118738876175	25.924517118691732	25.769031947370102	25.10533219506199
88-89	23.164349407386663	25.527633359086906	25.926285176400732	25.3817320571257
90-91	23.309434141238437	25.265534545188174	26.08975539377462	25.33527591979877
92-93	23.436118868988213	25.196088514932185	26.020455657609208	25.34733695847039
94-95	23.226587147822304	25.348684333395642	25.940886154133995	25.48384236464806
96-97	22.919352909117492	25.2198393058427	26.107189003163505	25.7536187818763
98-99	22.620255994944504	25.069717460684398	26.085829996569437	26.224196547801654
100-101	22.659475094656052	25.294601408164446	26.076279387794383	25.969644109385115
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	51.0
1	43.5
2	53.5
3	67.0
4	57.0
5	66.5
6	121.0
7	292.0
8	455.0
9	498.5
10	456.0
11	357.5
12	355.0
13	389.0
14	406.5
15	465.0
16	518.5
17	589.5
18	661.5
19	729.5
20	787.5
21	899.5
22	1039.5
23	1199.0
24	1451.0
25	1833.0
26	2349.5
27	3201.0
28	4543.5
29	6693.0
30	10240.5
31	15085.5
32	21983.5
33	28832.0
34	35086.5
35	42102.5
36	51165.5
37	60167.0
38	66914.0
39	73417.0
40	78746.5
41	83431.5
42	89161.5
43	96126.0
44	103053.0
45	110528.0
46	117367.0
47	123691.5
48	130279.5
49	134723.0
50	136393.0
51	135120.0
52	133114.5
53	131876.5
54	129075.5
55	122705.0
56	113577.5
57	101735.5
58	89766.0
59	77386.0
60	63959.5
61	53127.5
62	44092.5
63	34469.5
64	25084.5
65	18404.0
66	13964.5
67	10640.0
68	8306.0
69	6610.5
70	5141.0
71	3890.5
72	2834.0
73	2217.5
74	1775.0
75	1327.0
76	963.5
77	616.0
78	352.5
79	209.0
80	136.0
81	94.0
82	61.5
83	30.0
84	19.0
85	16.0
86	13.5
87	12.0
88	9.0
89	5.0
90	5.0
91	4.5
92	3.5
93	1.5
94	1.0
95	1.0
96	0.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15061310233043154
2	4.3743751793914437E-4
3	0.006124125251148021
4	0.0041388319005011345
5	0.026279900116190132
6	0.001043120235085652
7	1.3459615936589056E-4
8	0.001716101031915105
9	0.0
10-11	0.10072840076544837
12-13	2.1871875896957218E-4
14-15	0.005636214173446668
16-17	3.364903984147264E-5
18-19	1.8506971912809954E-4
20-21	5.21560117542826E-4
22-23	2.1871875896957218E-4
24-25	0.0013459615936589058
26-27	0.0022713101892994033
28-29	0.005838108412495504
30-31	0.013493264976430532
32-33	0.014065298653735564
34-35	0.011592094225387326
36-37	4.7108655778061706E-4
38-39	2.0189423904883586E-4
40-41	0.0042734280598670255
42-43	0.007857050802983863
44-45	0.007756103683459444
46-47	0.008042120522111961
48-49	0.010027413872758848
50-51	0.005131478575824578
52-53	0.0010262957151649156
54-55	7.402788765123982E-4
56-57	0.005282899255111205
58-59	0.009320784036087923
60-61	0.007301841645599563
62-63	0.017951762755425656
64-65	0.03502865047497302
66-67	0.03699711930569917
68-69	0.02777728238913567
70-71	0.04196035268231639
72-73	0.026464969835318236
74-75	0.02506853468189712
76-77	0.23840344727683369
78-79	0.06845897155747609
80-81	0.054208603184612424
82-83	0.020054827745517697
84-85	0.03289193644503951
86-87	0.0820363591335103
88-89	0.2645824002734994
90-91	0.31897607317723997
92-93	0.05298041323039868
94-95	0.004929584336775742
96-97	0.010347079751252838
98-99	0.0012281899542137514
100-101	0.0013796106335003783
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2971853.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.019759733816
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.92905051435962	44.63405027393739
2	12.937875727937165	15.01304882401768
3	4.10303849641162	7.141719232211996
4	1.9355843399709762	4.492085533986114
5	1.0710308358799416	3.107047588263117
6	0.6420575573218855	2.2351215126657955
7	0.4528576875336552	1.8392285957019934
8	0.3208672509584521	1.4893312645647547
9	0.2354073627914881	1.2292450765860057
>10	1.2806532559721533	13.301845683741693
>50	0.06864801184132927	2.6843255143303377
>100	0.021592086360214664	2.197052544756169
>500	9.88123271674763E-4	0.37565081566946285
>1k	3.487493898107395E-4	0.260247539567504
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.3649039841472646E-5
2	0.0	0.0	0.0	0.0	3.3649039841472646E-5
3	0.0	0.0	0.0	0.0	3.3649039841472646E-5
4	0.0	0.0	0.0	0.0	3.3649039841472646E-5
5	0.0	0.0	0.0	0.0	3.3649039841472646E-5
6	0.0	0.0	0.0	0.0	3.3649039841472646E-5
7	0.0	0.0	0.0	0.0	3.3649039841472646E-5
8	0.0	0.0	0.0	0.0	3.3649039841472646E-5
9	0.0	0.0	0.0	3.3649039841472646E-5	3.3649039841472646E-5
10-11	0.0	0.0	0.0	3.3649039841472646E-5	3.3649039841472646E-5
12-13	0.0	0.0	0.0	3.3649039841472646E-5	1.0094711952441793E-4
14-15	0.0	0.0	0.0	3.3649039841472646E-5	1.514206792866269E-4
16-17	0.0	0.0	0.0	3.3649039841472646E-5	1.6824519920736322E-4
18-19	0.0	0.0	0.0	3.3649039841472646E-5	2.0189423904883586E-4
20-21	0.0	0.0	0.0	3.3649039841472646E-5	2.6919231873178117E-4
22-23	0.0	0.0	0.0	1.1777163944515426E-4	3.3649039841472645E-4
24-25	0.0	0.0	0.0	4.3743751793914437E-4	4.037884780976717E-4
26-27	0.0	0.0	0.0	8.748750358782887E-4	4.71086557780617E-4
28-29	0.0	0.0	0.0	0.0025236779881104483	5.047355976220897E-4
30-31	0.0	0.0	0.0	0.010010589352838111	5.047355976220897E-4
32-33	0.0	0.0	0.0	0.02903912138319089	5.383846374635623E-4
34-35	0.0	0.0	0.0	0.056648158573119195	5.72033677305035E-4
36-37	0.0	0.0	0.0	0.09647179722550206	5.72033677305035E-4
38-39	0.0	0.0	0.0	0.1609265330418429	6.056827171465076E-4
40-41	0.0	0.0	0.0	0.23510584137236937	6.056827171465076E-4
42-43	0.0	0.0	0.0	0.31616637835047695	6.393317569879803E-4
44-45	0.0	0.0	0.0	0.42211038029135356	7.234543565916619E-4
46-47	0.0	0.0	0.0	0.5442900439557408	8.075769561953435E-4
48-49	0.0	0.0	0.0	0.6612036328849374	8.748750358782887E-4
50-51	0.0	0.0	0.0	0.7832150513501173	8.748750358782887E-4
52-53	0.0	0.0	0.0	0.9156240231263121	9.42173115561234E-4
54-55	0.0	0.0	0.0	1.0802687750706377	0.0010094711952441794
56-57	0.0	0.0	0.0	1.2752649609519717	0.0010262957151649156
58-59	0.0	0.0	0.0	1.4740971373752334	0.001043120235085652
60-61	0.0	0.0	0.0	1.6786160015317044	0.0010767692749271247
62-63	0.0	0.0	0.0	1.897250637901673	0.0011777163944515425
64-65	0.0	0.0	0.0	2.1515700810235234	0.0012450144741344878
66-67	0.0	0.0	0.0	2.453368319361691	0.0012618389940552241
68-69	0.0	0.0	0.0	2.7760794359613348	0.0012786635139759605
70-71	0.0	0.0	0.0	3.104914677812126	0.0013291370737381694
72-73	0.0	0.0	0.0	3.444820453770762	0.00146373323310406
74-75	0.0	0.0	0.0	3.827174493489416	0.0014805577530247963
76-77	0.0	0.0	0.0	4.276204105653948	0.0015142067928662689
78-79	0.0	0.0	0.0	4.768018471976911	0.0015142067928662689
80-81	0.0	0.0	0.0	5.261818131650523	0.0015310313127870053
82-83	0.0	0.0	0.0	5.7738555709182116	0.0016488029522321594
84-85	0.0	0.0	0.0	6.32310884825057	0.0016656274721528958
86-87	0.0	0.0	0.0	6.932913572777657	0.0016992765119943683
88-89	0.0	0.0	0.0	7.601267626628908	0.0017161010319151047
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3175	0.0	54.9344	1
GTATCAA	5920	0.0	44.79552	1
TCAACGC	7725	0.0	33.727867	4
ATCAACG	7950	0.0	32.952393	3
TATCAAC	7985	0.0	32.86739	2
CAACGCA	7950	0.0	32.830215	5
AACGCAG	8460	0.0	31.129965	6
GTACATG	12235	0.0	25.442476	1
ACGCAGA	10435	0.0	24.599892	7
TACATGG	12865	0.0	23.79386	2
CGCAGAG	10800	0.0	23.41638	8
ACATGGG	13120	0.0	22.318567	3
GTATAGG	670	0.0	21.989178	1
GTAAGTA	2235	0.0	20.384907	2
GCAGAGT	11775	0.0	20.185566	9
CATGGGG	10720	0.0	20.143347	4
AGAGTAC	10370	0.0	17.946712	10-11
TGTAAGT	2405	0.0	17.78482	1
CAGAGTA	10875	0.0	17.506735	10-11
TAAGTAC	3110	0.0	17.243803	3
>>END_MODULE