FastQCFastQC Report
Mon 15 Aug 2016
SRR2921355_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921355_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3643208
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34800.057.1430661
GTATCAA63050.042.153311
TCAACGC76350.033.9400374
CAACGCA79700.032.4525685
TATCAAC80800.032.072582
ATCAACG80750.031.9731273
AACGCAG84750.030.677966
GTACATG127200.028.7297841
TACATGG130400.027.189152
ACGCAGA101000.025.368797
ACATGGG138650.024.9880143
CGCAGAG104250.024.3971988
CATGGGG117350.023.0526334
ATGGGGG80300.021.690095
GAGTACA99800.020.401191
GCAGAGT118650.020.0764649
GTATATA12400.019.1371421
AGTACAT98300.019.1202812
CAGAGTA107850.018.54533210-11
AGAGTAC100000.017.7234310-11
Produced by FastQC (version 0.11.5)