##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921355_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3643208
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.358380032103575	31.0	31.0	34.0	26.0	34.0
2	30.402566913555304	31.0	31.0	34.0	26.0	34.0
3	30.450552370328566	33.0	31.0	34.0	26.0	34.0
4	33.914593676781564	37.0	35.0	37.0	30.0	37.0
5	33.906393760663676	37.0	35.0	37.0	30.0	37.0
6	33.95753138442823	37.0	35.0	37.0	30.0	37.0
7	33.87528244338506	37.0	35.0	37.0	30.0	37.0
8	33.84175265315623	37.0	35.0	37.0	29.0	37.0
9	35.391619693413055	39.0	37.0	39.0	30.0	39.0
10-11	35.284608509862736	39.0	35.0	39.0	29.0	39.0
12-13	35.16277632240597	39.0	35.0	39.0	28.0	39.0
14-15	36.282466578905186	40.0	36.5	41.0	27.5	41.0
16-17	36.22291754958816	40.0	36.0	41.0	27.0	41.0
18-19	36.172467369417284	40.0	36.5	41.0	27.0	41.0
20-21	36.11213894457851	40.0	36.0	41.0	27.0	41.0
22-23	36.000706245704336	40.0	36.0	41.0	27.0	41.0
24-25	35.90201135921968	40.0	36.0	41.0	27.0	41.0
26-27	35.74715443093011	40.0	36.0	41.0	27.0	41.0
28-29	35.604710875689776	40.0	36.0	41.0	26.0	41.0
30-31	35.452992664706485	40.0	35.5	41.0	25.0	41.0
32-33	35.26728874662111	39.0	35.0	41.0	25.0	41.0
34-35	35.10739600923142	39.0	35.0	41.0	24.0	41.0
36-37	34.936687940957526	39.0	35.0	41.0	24.0	41.0
38-39	35.17133608621852	39.0	35.0	41.0	24.5	41.0
40-41	35.29183401002633	40.0	35.0	41.0	24.5	41.0
42-43	35.12694375396629	40.0	35.0	41.0	24.0	41.0
44-45	35.00798197632416	40.0	35.0	41.0	23.0	41.0
46-47	34.85165999306106	40.0	35.0	41.0	22.5	41.0
48-49	34.7034220939348	39.0	35.0	41.0	21.5	41.0
50-51	32.99637023194943	37.0	32.5	39.5	17.0	40.0
52-53	33.152536171418156	37.5	33.0	39.5	16.5	40.0
54-55	34.011612430583156	39.0	34.0	40.5	18.0	41.0
56-57	34.02530228853252	39.0	34.0	41.0	18.0	41.0
58-59	33.84883555921046	38.0	34.0	41.0	16.0	41.0
60-61	33.595720996440505	38.0	34.0	40.0	13.5	41.0
62-63	33.30259581665389	37.0	33.5	40.0	12.0	41.0
64-65	33.016023515539054	37.0	33.0	40.0	8.0	41.0
66-67	32.6814653459259	36.0	33.0	39.0	7.5	41.0
68-69	32.31889024727657	36.0	33.0	39.0	4.0	41.0
70-71	31.94173376870055	35.0	32.0	39.0	2.0	40.5
72-73	31.58898407667089	35.0	32.0	37.5	2.0	40.0
74-75	31.201005542368158	35.0	32.0	37.0	2.0	39.0
76-77	30.831183259369215	35.0	32.0	36.5	2.0	39.0
78-79	30.494490020882694	35.0	31.0	36.0	2.0	38.5
80-81	30.15129427142233	35.0	31.0	36.0	2.0	37.0
82-83	29.859809129755973	35.0	31.0	35.0	2.0	37.0
84-85	29.63135003546325	35.0	31.0	35.0	2.0	36.5
86-87	29.411185828533533	35.0	31.0	35.0	2.0	36.0
88-89	29.217746145704552	35.0	30.5	35.0	2.0	36.0
90-91	28.970362795646032	34.5	30.0	35.0	2.0	35.5
92-93	28.777489097520643	34.0	30.0	35.0	2.0	35.0
94-95	28.567261188491024	34.0	30.0	35.0	2.0	35.0
96-97	28.316740905268105	34.0	30.0	35.0	2.0	35.0
98-99	28.09194685013867	34.0	29.5	35.0	2.0	35.0
100-101	27.05717996337294	33.0	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	129940.0
3	22163.0
4	15152.0
5	11582.0
6	10367.0
7	10711.0
8	11229.0
9	11877.0
10	12890.0
11	13768.0
12	13946.0
13	13963.0
14	15042.0
15	16335.0
16	16725.0
17	17569.0
18	15945.0
19	14947.0
20	15118.0
21	15587.0
22	17038.0
23	19704.0
24	23287.0
25	27481.0
26	32740.0
27	38598.0
28	46159.0
29	54375.0
30	65139.0
31	79412.0
32	99299.0
33	128984.0
34	184568.0
35	263208.0
36	427729.0
37	774532.0
38	810713.0
39	145364.0
40	22.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.74075119689101	17.554099363717828	12.804049313035504	24.90110012635566
2	16.150499230706274	19.217731822174226	39.60401559790409	25.02775334921541
3	19.68450180043017	23.37076997774453	29.014827459690796	27.9299007621345
4	13.634652128310822	15.30416269468217	36.12947309994955	34.93171207705746
5	14.461628029917106	37.388610683542986	33.79990360836987	14.349857678170041
6	34.04146207143442	34.30355810394783	17.13597971535388	14.519000109263875
7	29.49234621044078	31.924016647266818	20.23735532470955	18.346281817582852
8	27.334031944868077	34.61278810519146	19.550617260902854	18.50256268903761
9	27.72564869727936	15.429570284209493	18.983300818623928	37.86148019988722
10-11	26.326335573978067	24.976128403109595	27.159041116800882	21.538494906111456
12-13	26.653320396154427	22.27323969802999	27.212307013093127	23.861132892722452
14-15	23.90653685399562	23.944198803467646	24.526812548253083	27.62245179428365
16-17	24.07799066157005	27.001096054697303	25.320552068913067	23.600361214819582
18-19	24.065787349562317	26.56141382593541	26.554289347710778	22.818509476791498
20-21	24.506439930520713	25.590485406425422	26.76144176799063	23.14163289506324
22-23	24.44589591842283	25.30548462189713	26.40766506412589	23.84095439555415
24-25	24.244150460623164	25.452474491974485	26.318526774742658	23.984848272659697
26-27	23.832878903966062	25.835768160437617	26.4105580454865	23.92079489010982
28-29	23.883669997041853	26.30294037102443	26.019411211996214	23.7939784199375
30-31	24.636189617606195	25.70750553906472	25.975310499200745	23.680994344128344
32-33	24.22326267285154	25.74110604123237	25.948936908467125	24.086694377448964
34-35	24.4603338981341	25.97456269116927	26.104103143737333	23.461000266959292
36-37	24.41683468717306	25.902442016970724	26.19558067296862	23.485142622887597
38-39	24.37139409417889	25.970770648880503	25.9847152689747	23.6731199879659
40-41	24.511374722144296	25.689622895804042	25.976989787207412	23.82201259484425
42-43	24.1523512816214	25.77198415548432	25.80924223071482	24.266422332179467
44-45	23.949034339189907	25.867018482717658	26.148576739990702	24.035370438101733
46-47	24.179033260545523	25.70526712392812	25.843050597611754	24.272649017914603
48-49	23.984076892931544	25.91467799709864	25.996452364746407	24.10479274522341
50-51	24.157804484220087	25.85347763630706	26.08988653115563	23.898831348317216
52-53	24.348447866891267	25.689229605692876	26.01608855581147	23.946233971604386
54-55	23.647607409339628	25.526329793414515	26.48458436423024	24.341478433015613
56-57	23.84388462960755	25.721883207906515	26.082548049107956	24.351684113377974
58-59	24.030174977409473	25.74733015092577	26.23577511811639	23.986719753548375
60-61	24.363695623473497	25.72011078839087	26.34420313772086	23.571990450414766
62-63	24.205056624012432	25.650354458754205	26.339579050146806	23.805009867086564
64-65	23.83107010168865	25.401740577874936	26.40644270153177	24.36074661890465
66-67	23.947328217927772	25.590622804591845	26.171073422492885	24.290975554987494
68-69	23.873288232090214	25.625163375011123	26.32538815702537	24.176160235873294
70-71	23.817185629779825	25.46948928046336	26.25220284697607	24.461122242780753
72-73	23.725966010360814	25.947008523740127	26.027319471891925	24.299705994007134
74-75	23.672384921648124	25.915358716726182	26.204454461370464	24.20780190025523
76-77	23.73965215255988	25.45020844530368	26.16274889040222	24.64739051173422
78-79	23.654908257896416	25.501426359800238	26.315722881732267	24.527942500571083
80-81	23.93010491442256	25.747790850036374	25.980461801706912	24.341642433834153
82-83	23.9270140414954	25.813471220394568	26.14907030485177	24.110444433258266
84-85	23.94698609202522	25.85443661204771	25.944448967601602	24.254128328325464
86-87	23.542637782971738	26.106150990153488	25.959685743462437	24.391525483412344
88-89	23.452595381785045	25.773559601795014	26.241130654562333	24.532714361857604
90-91	23.789877323397576	25.580398923161653	26.253766760951457	24.37595699248931
92-93	24.064178111012087	25.38676979152238	26.233137131454342	24.315914966011192
94-95	23.885301994948282	25.468541221323743	26.205292431324388	24.440864352403583
96-97	23.681092071667546	25.330081220285262	26.332031937841172	24.656794770206023
98-99	23.34968438899914	25.305456069553955	26.32338390784866	25.02147563359825
100-101	23.585105652699003	25.47034748368688	26.347176635718057	24.597370227896064
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	78.0
1	68.5
2	94.0
3	140.0
4	132.0
5	161.5
6	275.5
7	494.5
8	739.5
9	872.5
10	805.0
11	697.0
12	712.0
13	786.5
14	897.0
15	1007.0
16	1127.5
17	1244.0
18	1428.0
19	1619.0
20	1741.5
21	1913.0
22	2106.0
23	2385.0
24	2741.5
25	3294.5
26	4382.5
27	6218.5
28	8732.5
29	12367.5
30	17498.0
31	24064.0
32	32892.0
33	40187.0
34	46081.5
35	53680.5
36	63322.5
37	72945.5
38	80648.5
39	88289.5
40	95216.0
41	101320.0
42	108307.5
43	116572.5
44	123979.0
45	131630.0
46	138205.0
47	144434.0
48	152376.0
49	158707.5
50	161568.5
51	160906.0
52	159827.5
53	159483.0
54	156120.5
55	148338.0
56	138532.5
57	125824.0
58	111775.5
59	96067.5
60	79373.0
61	66484.5
62	55270.0
63	43076.5
64	31660.0
65	23485.0
66	17640.5
67	13322.5
68	10356.0
69	8131.5
70	6393.5
71	4838.0
72	3579.0
73	2857.0
74	2331.0
75	1764.0
76	1309.0
77	872.0
78	571.0
79	403.0
80	284.5
81	218.0
82	172.5
83	135.0
84	105.0
85	84.5
86	76.0
87	59.0
88	48.0
89	53.0
90	51.5
91	43.5
92	29.5
93	22.5
94	24.0
95	17.0
96	14.0
97	10.0
98	5.5
99	5.5
100	6.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05212439147037446
2	0.060551030849734636
3	0.05094411299052923
4	0.05711998875716127
5	0.049763834510683995
6	0.017841418881381462
7	0.015590655268653341
8	0.020449010871737217
9	0.0206960459024025
10-11	0.022068462739431843
12-13	0.027228750046662174
14-15	0.043958511290049865
16-17	0.028930546924578558
18-19	0.02294680951513062
20-21	0.02666605914348014
22-23	0.04663472412225709
24-25	0.04147443681502676
26-27	0.030042204562572325
28-29	0.019913768305295773
30-31	0.012214509849561156
32-33	0.00938733116528071
34-35	0.009071675292763958
36-37	0.0014135893421402237
38-39	0.006203324103372632
40-41	0.0035682837762762926
42-43	0.2128481272548809
44-45	0.18769172663213302
46-47	0.19400484408246796
48-49	0.1916717354595181
50-51	0.19003855942345316
52-53	0.19441656913357677
54-55	0.19250890973010598
56-57	0.20373527945700604
58-59	0.19986506397658327
60-61	0.20443521204389098
62-63	0.20265107015575282
64-65	0.2032274852273052
66-67	0.19399111991409768
68-69	0.19264615141380892
70-71	0.17668494359915765
72-73	0.18438420205489228
74-75	0.18380778698333997
76-77	0.18232557679934827
78-79	0.20735845990676346
80-81	0.21454992413279722
82-83	0.21324612813761937
84-85	0.20849756588149784
86-87	0.2255155346606617
88-89	0.22358042692045033
90-91	0.22293539100704654
92-93	0.22676443398235838
94-95	0.23612431681089854
96-97	0.2373732161325952
98-99	0.22712126235998603
100-101	0.2370026635865973
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3643208.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.574547259483346
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.72612434274019	43.407457476653505
2	12.698978539790124	14.36877923093042
3	4.2689795420150745	7.245467545484982
4	2.031379307008018	4.596974584250465
5	1.1416282382120277	3.229355035774354
6	0.6689087798482639	2.2705926826685365
7	0.44225989589559805	1.7514457366923784
8	0.32553438958060693	1.473356856633209
9	0.2559656508689167	1.303302673069915
>10	1.3316552229935164	13.50853091387817
>50	0.07752651150267899	3.014258178345897
>100	0.02881541334886815	2.6859380766531302
>500	0.0016099453270981853	0.6327157888342932
>1k	6.342208690575287E-4	0.5118252201308013
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.7448336740586867E-5
2	0.0	0.0	0.0	0.0	2.7448336740586867E-5
3	0.0	0.0	0.0	0.0	5.4896673481173734E-5
4	0.0	0.0	0.0	2.7448336740586867E-5	1.0979334696234747E-4
5	0.0	2.7448336740586867E-5	0.0	2.7448336740586867E-5	1.0979334696234747E-4
6	0.0	2.7448336740586867E-5	0.0	2.7448336740586867E-5	1.3724168370293432E-4
7	0.0	2.7448336740586867E-5	0.0	2.7448336740586867E-5	1.646900204435212E-4
8	0.0	2.7448336740586867E-5	0.0	2.7448336740586867E-5	2.1958669392469494E-4
9	0.0	2.7448336740586867E-5	0.0	2.7448336740586867E-5	2.1958669392469494E-4
10-11	0.0	2.7448336740586867E-5	0.0	2.7448336740586867E-5	2.1958669392469494E-4
12-13	0.0	2.7448336740586867E-5	0.0	5.4896673481173734E-5	2.7448336740586865E-4
14-15	0.0	2.7448336740586867E-5	0.0	5.4896673481173734E-5	3.7055254599792267E-4
16-17	0.0	2.7448336740586867E-5	0.0	5.4896673481173734E-5	4.6662172458997674E-4
18-19	0.0	2.7448336740586867E-5	0.0	5.4896673481173734E-5	5.215183980711505E-4
20-21	0.0	2.7448336740586867E-5	0.0	8.23450102217606E-5	5.215183980711505E-4
22-23	0.0	2.7448336740586867E-5	0.0	1.3724168370293432E-4	5.764150715523242E-4
24-25	0.0	2.7448336740586867E-5	0.0	2.7448336740586865E-4	7.136567552552585E-4
26-27	0.0	2.7448336740586867E-5	0.0	5.489667348117373E-4	7.411050919958454E-4
28-29	0.0	2.7448336740586867E-5	0.0	0.0019762802453222542	7.411050919958454E-4
30-31	0.0	2.7448336740586867E-5	0.0	0.007809051802696964	7.411050919958454E-4
32-33	1.3724168370293433E-5	2.7448336740586867E-5	0.0	0.021574392678101278	8.508984389581929E-4
34-35	2.7448336740586867E-5	2.7448336740586867E-5	0.0	0.04052746919747651	9.332434491799535E-4
36-37	2.7448336740586867E-5	2.7448336740586867E-5	0.0	0.07094022630604675	9.46967617550247E-4
38-39	2.7448336740586867E-5	2.7448336740586867E-5	0.0	0.12218627100072244	9.606917859205404E-4
40-41	4.11725051108803E-5	2.7448336740586867E-5	0.0	0.1843018570446705	9.881401226611273E-4
42-43	5.4896673481173734E-5	2.7448336740586867E-5	0.0	0.24695268565506004	9.881401226611273E-4
44-45	5.4896673481173734E-5	2.7448336740586867E-5	0.0	0.32626465466698584	0.0010155884594017141
46-47	5.4896673481173734E-5	2.7448336740586867E-5	0.0	0.41707747677321744	0.001043036796142301
48-49	5.4896673481173734E-5	2.7448336740586867E-5	0.0	0.5139563812991188	0.0010567609645125943
50-51	5.4896673481173734E-5	2.7448336740586867E-5	0.0	0.6231458648531734	0.0010979334696234746
52-53	5.4896673481173734E-5	2.7448336740586867E-5	0.0	0.7378250157553452	0.001207726816585822
54-55	5.4896673481173734E-5	4.11725051108803E-5	0.0	0.8737354551263612	0.0012763476584372892
56-57	5.4896673481173734E-5	5.4896673481173734E-5	0.0	1.028063728450311	0.001331244331918463
58-59	5.4896673481173734E-5	5.4896673481173734E-5	0.0	1.2000138339617172	0.0013724168370293434
60-61	5.4896673481173734E-5	5.4896673481173734E-5	0.0	1.3814610639853666	0.0013998651737699302
62-63	5.4896673481173734E-5	5.4896673481173734E-5	0.0	1.5780186033847095	0.0015096585207322777
64-65	5.4896673481173734E-5	5.4896673481173734E-5	0.0	1.791978388277584	0.0015233826891025712
66-67	6.862084185146716E-5	5.4896673481173734E-5	0.0	2.0405779741370793	0.0015508310258431578
68-69	8.23450102217606E-5	5.4896673481173734E-5	0.0	2.316200447517682	0.0015645551942134513
70-71	8.23450102217606E-5	5.4896673481173734E-5	0.0	2.608044887911972	0.0016606243728055055
72-73	8.23450102217606E-5	5.4896673481173734E-5	0.0	2.9184855764480098	0.001962556076951961
74-75	8.23450102217606E-5	5.4896673481173734E-5	0.0	3.245244301176326	0.0020311769188034283
76-77	8.23450102217606E-5	5.4896673481173734E-5	0.0	3.6100875931322065	0.0020449010871737214
78-79	8.23450102217606E-5	5.4896673481173734E-5	0.0	4.02462609875692	0.002058625255544015
80-81	8.23450102217606E-5	5.4896673481173734E-5	0.0	4.4581039567326375	0.0020723494239143084
82-83	8.23450102217606E-5	5.4896673481173734E-5	0.0	4.916326490279995	0.0021409702657657756
84-85	8.23450102217606E-5	5.4896673481173734E-5	0.0	5.389096642299863	0.0021409702657657756
86-87	8.23450102217606E-5	5.4896673481173734E-5	0.0	5.9039176462063105	0.0021958669392469496
88-89	8.23450102217606E-5	5.4896673481173734E-5	0.0	6.458758325080534	0.0022644877810984168
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3480	0.0	57.143066	1
GTATCAA	6305	0.0	42.15331	1
TCAACGC	7635	0.0	33.940037	4
CAACGCA	7970	0.0	32.452568	5
TATCAAC	8080	0.0	32.07258	2
ATCAACG	8075	0.0	31.973127	3
AACGCAG	8475	0.0	30.67796	6
GTACATG	12720	0.0	28.729784	1
TACATGG	13040	0.0	27.18915	2
ACGCAGA	10100	0.0	25.36879	7
ACATGGG	13865	0.0	24.988014	3
CGCAGAG	10425	0.0	24.397198	8
CATGGGG	11735	0.0	23.052633	4
ATGGGGG	8030	0.0	21.69009	5
GAGTACA	9980	0.0	20.40119	1
GCAGAGT	11865	0.0	20.076464	9
GTATATA	1240	0.0	19.137142	1
AGTACAT	9830	0.0	19.120281	2
CAGAGTA	10785	0.0	18.545332	10-11
AGAGTAC	10000	0.0	17.72343	10-11
>>END_MODULE