FastQCFastQC Report
Mon 15 Aug 2016
SRR2921355_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921355_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3643208
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34500.051.1056371
GTATCAA64200.044.8592031
TCAACGC82650.033.9930574
CAACGCA85500.032.9747625
ATCAACG87050.032.4384083
TATCAAC87500.032.1635482
AACGCAG90500.031.044476
GTACATG124600.026.0123921
ACGCAGA110000.025.2811367
TACATGG129400.024.9397412
CGCAGAG113300.024.2091378
ACATGGG136750.023.0436923
CATGGGG115100.021.069634
GCAGAGT125700.020.9133329
GAGTACA99600.019.4199921
ATGGGGG76250.019.3916025
AGAGTAC109600.018.99752210-11
GTATATG12650.018.7842311
TACCGTA2552.656834E-718.642037
CGTATAC2603.3154356E-718.2531573
Produced by FastQC (version 0.11.5)